{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T08:38:03Z","timestamp":1793522283909,"version":"4.5.0"},"reference-count":77,"publisher":"Elsevier BV","issue":"21","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T00:00:00Z","timestamp":1629849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070636"],"award-info":[{"award-number":["32070636"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82003086"],"award-info":[{"award-number":["82003086"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003347","name":"Fudan University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["20ZR1412100"],"award-info":[{"award-number":["20ZR1412100"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Cell"],"published-print":{"date-parts":[[2021,11]]},"DOI":"10.1016\/j.molcel.2021.08.029","type":"journal-article","created":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T10:34:24Z","timestamp":1631788464000},"page":"4425-4439.e6","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":128,"title":["SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape"],"prefix":"10.1016","volume":"81","author":[{"given":"Shibin","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linna","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congling","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenning","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aixia","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei Xavier","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.molcel.2021.08.029_bib1","doi-asserted-by":"crossref","first-page":"9354","DOI":"10.1038\/s41598-019-45839-z","article-title":"The ENCODE Blacklist: Identification of Problematic Regions of the Genome","volume":"9","author":"Amemiya","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.molcel.2021.08.029_bib2","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.tig.2015.05.007","article-title":"A unified architecture of transcriptional regulatory elements","volume":"31","author":"Andersson","year":"2015","journal-title":"Trends Genet."},{"key":"10.1016\/j.molcel.2021.08.029_bib3","doi-asserted-by":"crossref","first-page":"2635","DOI":"10.1101\/gad.844200","article-title":"High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in\u00a0vivo: roles in promoter proximal pausing and transcription elongation","volume":"14","author":"Andrulis","year":"2000","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2021.08.029_bib4","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.molcel.2020.02.014","article-title":"NELF Regulates a Promoter-Proximal Step Distinct from RNA Pol II Pause-Release","volume":"78","author":"Aoi","year":"2020","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib5","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.molcel.2017.02.009","article-title":"Genome-wide Analysis of RNA Polymerase II Termination at Protein-Coding Genes","volume":"66","author":"Baejen","year":"2017","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib6","doi-asserted-by":"crossref","first-page":"107917","DOI":"10.1016\/j.celrep.2020.107917","article-title":"The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts","volume":"32","author":"Beckedorff","year":"2020","journal-title":"Cell Rep."},{"key":"10.1016\/j.molcel.2021.08.029_bib7","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1038\/nsmb.3465","article-title":"Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp","volume":"24","author":"Bernecky","year":"2017","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib8","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/j.molcel.2009.10.019","article-title":"Progression through the RNA polymerase II CTD cycle","volume":"36","author":"Buratowski","year":"2009","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib9","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1101\/gad.246173.114","article-title":"Stably paused genes revealed through inhibition of transcription initiation by the TFIIH inhibitor triptolide","volume":"29","author":"Chen","year":"2015","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2021.08.029_bib10","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1126\/science.aan3269","article-title":"PAF1 regulation of promoter-proximal pause release via enhancer activation","volume":"357","author":"Chen","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.molcel.2021.08.029_bib11","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1038\/s41580-018-0010-5","article-title":"Born to run: control of transcription elongation by RNA polymerase II","volume":"19","author":"Chen","year":"2018","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib12","doi-asserted-by":"crossref","first-page":"eaba8490","DOI":"10.1126\/science.aba8490","article-title":"Structural insights into preinitiation complex assembly on core promoters","volume":"372","author":"Chen","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.molcel.2021.08.029_bib13","doi-asserted-by":"crossref","first-page":"21901","DOI":"10.1074\/jbc.M702936200","article-title":"Properties of RNA polymerase II elongation complexes before and after the P-TEFb-mediated transition into productive elongation","volume":"282","author":"Cheng","year":"2007","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.molcel.2021.08.029_bib14","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1101\/gad.325142.119","article-title":"Promoter-proximal pausing of RNA polymerase II: a nexus of gene regulation","volume":"33","author":"Core","year":"2019","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2021.08.029_bib15","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1016\/j.molcel.2019.09.031","article-title":"Control of RNA Pol II Speed by PNUTS-PP1 and Spt5 Dephosphorylation Facilitates Termination by a \u201cSitting Duck Torpedo\u201d Mechanism","volume":"76","author":"Cortazar","year":"2019","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib16","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/s41586-019-1517-4","article-title":"Organization and regulation of gene transcription","volume":"573","author":"Cramer","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2021.08.029_bib17","doi-asserted-by":"crossref","first-page":"6362","DOI":"10.1093\/nar\/gkx220","article-title":"The elongation factor Spt4\/5 regulates RNA polymerase II transcription through the nucleosome","volume":"45","author":"Crickard","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2021.08.029_bib18","doi-asserted-by":"crossref","first-page":"166657","DOI":"10.1016\/j.jmb.2020.09.016","article-title":"Mechanisms of Transcription Elongation Factor DSIF (Spt4-Spt5)","volume":"433","author":"Decker","year":"2021","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib19","doi-asserted-by":"crossref","first-page":"38755","DOI":"10.1074\/jbc.M112.412015","article-title":"Cross-talk among RNA polymerase II kinases modulates C-terminal domain phosphorylation","volume":"287","author":"Devaiah","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.molcel.2021.08.029_bib20","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.molcel.2021.08.029_bib21","doi-asserted-by":"crossref","first-page":"166897","DOI":"10.1016\/j.jmb.2021.166897","article-title":"Regulation of Promoter Proximal Pausing of RNA Polymerase II in Metazoans","volume":"433","author":"Dollinger","year":"2021","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib22","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1016\/j.molcel.2019.10.034","article-title":"The Integrator Complex Attenuates Promoter-Proximal Transcription at Protein-Coding Genes","volume":"76","author":"Elrod","year":"2019","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib23","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/j.molcel.2020.03.008","article-title":"TFIID Enables RNA Polymerase II Promoter-Proximal Pausing","volume":"78","author":"Fant","year":"2020","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib24","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1038\/s41594-021-00578-6","article-title":"Structural basis of nucleosome transcription mediated by Chd1 and FACT","volume":"28","author":"Farnung","year":"2021","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib25","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1146\/annurev-biochem-011420-095916","article-title":"Evaluating Enhancer Function and Transcription","volume":"89","author":"Field","year":"2020","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.molcel.2021.08.029_bib26","doi-asserted-by":"crossref","first-page":"e97965","DOI":"10.15252\/embj.201797965","article-title":"Regulation of RNA polymerase II processivity by Spt5 is restricted to a narrow window during elongation","volume":"37","author":"Fitz","year":"2018","journal-title":"EMBO J."},{"key":"10.1016\/j.molcel.2021.08.029_bib27","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1038\/s41588-020-0605-6","article-title":"Spt5-mediated enhancer transcription directly couples enhancer activation with physical promoter interaction","volume":"52","author":"Fitz","year":"2020","journal-title":"Nat. Genet."},{"key":"10.1016\/j.molcel.2021.08.029_bib28","doi-asserted-by":"crossref","first-page":"166876","DOI":"10.1016\/j.jmb.2021.166876","article-title":"Collaboration through chromatin: motors of transcription and chromatin structure","volume":"433","author":"Gamarra","year":"2021","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib29","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.molcel.2014.08.004","article-title":"Integrator regulates transcriptional initiation and pause release following activation","volume":"56","author":"Gardini","year":"2014","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib30","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/s41580-018-0028-8","article-title":"Eukaryotic core promoters and the functional basis of transcription initiation","volume":"19","author":"Haberle","year":"2018","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib31","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.bbagrm.2012.08.007","article-title":"The Spt4-Spt5 complex: a multi-faceted regulator of transcription elongation","volume":"1829","author":"Hartzog","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.molcel.2021.08.029_bib32","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1126\/science.aaz9761","article-title":"Structure of nucleosome-bound human BAF complex","volume":"367","author":"He","year":"2020","journal-title":"Science"},{"key":"10.1016\/j.molcel.2021.08.029_bib33","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.molcel.2013.10.001","article-title":"Stable pausing by RNA polymerase II provides an opportunity to target and integrate regulatory signals","volume":"52","author":"Henriques","year":"2013","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib34","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1101\/gad.309351.117","article-title":"Widespread transcriptional pausing and elongation control at enhancers","volume":"32","author":"Henriques","year":"2018","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2021.08.029_bib35","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.1101\/gad.200303.112","article-title":"The RNA polymerase II CTD coordinates transcription and RNA processing","volume":"26","author":"Hsin","year":"2012","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2021.08.029_bib36","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.molcel.2020.08.016","article-title":"Integrator Recruits Protein Phosphatase 2A to Prevent Pause Release and Facilitate Transcription Termination","volume":"80","author":"Huang","year":"2020","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib37","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1016\/j.molcel.2021.02.015","article-title":"Fast-acting chemical tools to delineate causality in transcriptional control","volume":"81","author":"Jaeger","year":"2021","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib38","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/nrm3953","article-title":"Getting up to speed with transcription elongation by RNA polymerase II","volume":"16","author":"Jonkers","year":"2015","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib39","doi-asserted-by":"crossref","first-page":"e02407","DOI":"10.7554\/eLife.02407","article-title":"Genome-wide dynamics of Pol II elongation and its interplay with promoter proximal pausing, chromatin, and exons","volume":"3","author":"Jonkers","year":"2014","journal-title":"eLife"},{"key":"10.1016\/j.molcel.2021.08.029_bib40","article-title":"A rapid, sensitive, scalable method for precision run-on sequencing (PRO-seq)","author":"Judd","year":"2020","journal-title":"bioRxiv"},{"key":"10.1016\/j.molcel.2021.08.029_bib41","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.celrep.2018.09.007","article-title":"Elongation\/Termination Factor Exchange Mediated by PP1 Phosphatase Orchestrates Transcription Termination","volume":"25","author":"Kecman","year":"2018","journal-title":"Cell Rep."},{"key":"10.1016\/j.molcel.2021.08.029_bib42","doi-asserted-by":"crossref","first-page":"12317","DOI":"10.1074\/jbc.M010908200","article-title":"Positive transcription elongation factor B phosphorylates hSPT5 and RNA polymerase II carboxyl-terminal domain independently of cyclin-dependent kinase-activating kinase","volume":"276","author":"Kim","year":"2001","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.molcel.2021.08.029_bib43","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1111\/j.1365-2443.2008.01273.x","article-title":"Role of human transcription elongation factor DSIF in the suppression of senescence and apoptosis","volume":"14","author":"Komori","year":"2009","journal-title":"Genes Cells"},{"key":"10.1016\/j.molcel.2021.08.029_bib44","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1126\/science.1229386","article-title":"Precise maps of RNA polymerase reveal how promoters direct initiation and pausing","volume":"339","author":"Kwak","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.molcel.2021.08.029_bib45","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.molcel.2021.08.029_bib46","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The Sequence Alignment\/Map format and SAMtools","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.molcel.2021.08.029_bib47","doi-asserted-by":"crossref","first-page":"7069","DOI":"10.1093\/nar\/gku333","article-title":"Insights into how Spt5 functions in transcription elongation and repressing transcription coupled DNA repair","volume":"42","author":"Li","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2021.08.029_bib48","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkz114","article-title":"The R package Rsubread is easier, faster, cheaper and better for alignment and quantification of RNA sequencing reads","volume":"47","author":"Liao","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2021.08.029_bib49","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib50","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.molcel.2020.12.014","article-title":"Integrator is a genome-wide attenuator of non-productive transcription","volume":"81","author":"Lykke-Andersen","year":"2021","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib51","doi-asserted-by":"crossref","first-page":"11301","DOI":"10.1073\/pnas.1000681107","article-title":"Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex","volume":"107","author":"Missra","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.molcel.2021.08.029_bib52","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1038\/s41589-018-0021-8","article-title":"The dTAG system for immediate and target-specific protein degradation","volume":"14","author":"Nabet","year":"2018","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib53","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1128\/MCB.01859-07","article-title":"P-TEFb is critical for the maturation of RNA polymerase II into productive elongation in\u00a0vivo","volume":"28","author":"Ni","year":"2008","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib54","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1016\/j.celrep.2014.10.018","article-title":"Quantitative ChIP-Seq normalization reveals global modulation of the epigenome","volume":"9","author":"Orlando","year":"2014","journal-title":"Cell Rep."},{"key":"10.1016\/j.molcel.2021.08.029_bib55","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1186\/s13059-021-02322-1","article-title":"Mechanisms of enhancer action: the known and the unknown","volume":"22","author":"Panigrahi","year":"2021","journal-title":"Genome Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib56","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1038\/s41586-018-0214-z","article-title":"A Cdk9-PP1 switch regulates the elongation-termination transition of RNA polymerase II","volume":"558","author":"Parua","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2021.08.029_bib57","doi-asserted-by":"crossref","first-page":"4338","DOI":"10.1038\/s41467-020-18173-6","article-title":"Distinct Cdk9-phosphatase switches act at the beginning and end of elongation by RNA polymerase II","volume":"11","author":"Parua","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.molcel.2021.08.029_bib58","doi-asserted-by":"crossref","first-page":"5555","DOI":"10.1074\/jbc.M116.760751","article-title":"Identification of Regions in the Spt5 Subunit of DRB Sensitivity-inducing Factor (DSIF) That Are Involved in Promoter-proximal Pausing","volume":"292","author":"Qiu","year":"2017","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.molcel.2021.08.029_bib59","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: a flexible suite of utilities for comparing genomic features","volume":"26","author":"Quinlan","year":"2010","journal-title":"Bioinformatics"},{"issue":"W1","key":"10.1016\/j.molcel.2021.08.029_bib60","doi-asserted-by":"crossref","first-page":"W160","DOI":"10.1093\/nar\/gkw257","article-title":"deepTools2: a next generation web server for deep-sequencing data analysis","volume":"44","author":"Ram\u00edrez","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2021.08.029_bib61","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1101\/gad.341545.120","article-title":"Selective inhibition of CDK7 reveals high-confidence targets and new models for TFIIH function in transcription","volume":"34","author":"Rimel","year":"2020","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2021.08.029_bib62","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/s41594-019-0287-x","article-title":"50+ years of eukaryotic transcription: an expanding universe of factors and mechanisms","volume":"26","author":"Roeder","year":"2019","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib63","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/nature10730","article-title":"Differential oestrogen receptor binding is associated with clinical outcome in breast cancer","volume":"481","author":"Ross-Innes","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2021.08.029_bib64","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1038\/nprot.2015.140","article-title":"MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems","volume":"11","author":"Sakuma","year":"2016","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.molcel.2021.08.029_bib65","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/s41588-021-00777-3","article-title":"Acute BAF perturbation causes immediate changes in chromatin accessibility","volume":"53","author":"Schick","year":"2021","journal-title":"Nat. Genet."},{"key":"10.1016\/j.molcel.2021.08.029_bib66","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1126\/science.aad9841","article-title":"TT-seq maps the human transient transcriptome","volume":"352","author":"Schwalb","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.molcel.2021.08.029_bib67","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1038\/ng.3867","article-title":"Paused RNA polymerase II inhibits new transcriptional initiation","volume":"49","author":"Shao","year":"2017","journal-title":"Nat. Genet."},{"key":"10.1016\/j.molcel.2021.08.029_bib68","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.molcel.2017.02.023","article-title":"Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation","volume":"66","author":"Shetty","year":"2017","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib69","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1101\/gad.330167.119","article-title":"The Integrator complex cleaves nascent mRNAs to attenuate transcription","volume":"33","author":"Tatomer","year":"2019","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2021.08.029_bib70","doi-asserted-by":"crossref","first-page":"3143","DOI":"10.1016\/j.cell.2021.04.022","article-title":"The PP2A-Integrator-CDK9 axis fine-tunes transcription and can be targeted therapeutically in cancer","volume":"184","author":"Vervoort","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib71","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/s41586-018-0440-4","article-title":"Structure of activated transcription complex Pol II-DSIF-PAF-SPT6","volume":"560","author":"Vos","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2021.08.029_bib72","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1101\/gad.12.3.343","article-title":"DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs","volume":"12","author":"Wada","year":"1998","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2021.08.029_bib73","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.cell.2013.03.035","article-title":"Master transcription factors and mediator establish super-enhancers at key cell identity genes","volume":"153","author":"Whyte","year":"2013","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib74","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.molcel.2005.11.024","article-title":"P-TEFb-mediated phosphorylation of hSpt5 C-terminal repeats is critical for processive transcription elongation","volume":"21","author":"Yamada","year":"2006","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2021.08.029_bib75","doi-asserted-by":"crossref","first-page":"2382","DOI":"10.1093\/bioinformatics\/btv145","article-title":"ChIPseeker: an R\/Bioconductor package for ChIP peak annotation, comparison and visualization","volume":"31","author":"Yu","year":"2015","journal-title":"Bioinformatics"},{"key":"10.1016\/j.molcel.2021.08.029_bib76","doi-asserted-by":"crossref","first-page":"R137","DOI":"10.1186\/gb-2008-9-9-r137","article-title":"Model-based analysis of ChIP-Seq (MACS)","volume":"9","author":"Zhang","year":"2008","journal-title":"Genome Biol."},{"key":"10.1016\/j.molcel.2021.08.029_bib77","doi-asserted-by":"crossref","first-page":"eabb5872","DOI":"10.1126\/science.abb5872","article-title":"Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase","volume":"370","author":"Zheng","year":"2020","journal-title":"Science"}],"container-title":["Molecular Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1097276521006961?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1097276521006961?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:40:21Z","timestamp":1761093621000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1097276521006961"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":77,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["S1097276521006961"],"URL":"https:\/\/doi.org\/10.1016\/j.molcel.2021.08.029","relation":{},"ISSN":["1097-2765"],"issn-type":[{"value":"1097-2765","type":"print"}],"subject":[],"published":{"date-parts":[[2021,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape","name":"articletitle","label":"Article Title"},{"value":"Molecular Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.molcel.2021.08.029","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}