{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:45:44Z","timestamp":1792665944530,"version":"4.5.0"},"reference-count":77,"publisher":"Elsevier BV","issue":"19","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T00:00:00Z","timestamp":1759449600000},"content-version":"vor","delay-in-days":367,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Cell"],"published-print":{"date-parts":[[2024,10]]},"DOI":"10.1016\/j.molcel.2024.07.017","type":"journal-article","created":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T10:31:00Z","timestamp":1723199460000},"page":"3758-3774.e10","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":43,"title":["Nuclear retention coupled with sequential polyadenylation dictates post-transcriptional m6A modification in the nucleus"],"prefix":"10.1016","volume":"84","author":[{"given":"Peng","family":"Tang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zonggui","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiping","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangnan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feiyan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Shan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xichen","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2102-9377","authenticated-orcid":false,"given":"Yu","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.molcel.2024.07.017_bib1","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1038\/nature21355","article-title":"m6A-dependent maternal mRNA clearance facilitates zebrafish maternal-to-zygotic transition","volume":"542","author":"Zhao","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2024.07.017_bib2","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1126\/science.aau1646","article-title":"RNA modifications modulate gene expression during development","volume":"361","author":"Frye","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.molcel.2024.07.017_bib3","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1038\/s41590-020-0650-4","article-title":"The RNA modification N6-methyladenosine as a novel regulator of the immune system","volume":"21","author":"Shulman","year":"2020","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.molcel.2024.07.017_bib4","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1038\/nature15377","article-title":"Dynamic m(6)A mRNA methylation directs translational control of heat\u00a0shock response","volume":"526","author":"Zhou","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2024.07.017_bib5","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1126\/science.aay6018","article-title":"N6-methyladenosine of chromosome-associated regulatory RNA regulates chromatin state and transcription","volume":"367","author":"Liu","year":"2020","journal-title":"Science"},{"key":"10.1016\/j.molcel.2024.07.017_bib6","doi-asserted-by":"crossref","first-page":"3356","DOI":"10.1016\/j.molcel.2021.06.023","article-title":"m6A RNA methylation regulates promoter- proximal pausing of RNA polymerase II","volume":"81","author":"Akhtar","year":"2021","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib7","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/nature20577","article-title":"m6A potentiates Sxl alternative pre-mRNA splicing for robust Drosophila sex determination","volume":"540","author":"Haussmann","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2024.07.017_bib8","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1038\/nature20568","article-title":"m6A modulates neuronal functions and sex determination in Drosophila","volume":"540","author":"Lence","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2024.07.017_bib9","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.molcel.2016.01.012","article-title":"Nuclear m6A Reader YTHDC1 Regulates mRNA Splicing","volume":"61","author":"Xiao","year":"2016","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib10","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.cell.2017.05.003","article-title":"The U6 snRNA m6A Methyltransferase METTL16 Regulates SAM Synthetase Intron Retention","volume":"169","author":"Pendleton","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib11","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1101\/gr.271635.120","article-title":"Acute depletion of METTL3 implicates N6-methyladenosine in alternative intron\/exon inclusion in the nascent transcriptome","volume":"31","author":"Wei","year":"2021","journal-title":"Genome Res."},{"key":"10.1016\/j.molcel.2024.07.017_bib12","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.molcel.2012.10.015","article-title":"ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility","volume":"49","author":"Zheng","year":"2013","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib13","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.31311","article-title":"YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs","volume":"6","author":"Roundtree","year":"2017","journal-title":"eLife"},{"key":"10.1016\/j.molcel.2024.07.017_bib14","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/nature12730","article-title":"N6-methyladenosine-dependent regulation of messenger RNA stability","volume":"505","author":"Wang","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2024.07.017_bib15","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1101\/gad.301036.117","article-title":"m6A mRNA modifications are deposited in nascent pre-mRNA and are not required for splicing but do specify cytoplasmic turnover","volume":"31","author":"Ke","year":"2017","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2024.07.017_bib16","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.stem.2014.09.019","article-title":"m(6)A RNA modification controls cell fate transition in mammalian embryonic stem cells","volume":"15","author":"Batista","year":"2014","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib17","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1126\/science.1261417","article-title":"Stem cells. m6A mRNA methylation facilitates resolution of naive pluripotency toward differentiation","volume":"347","author":"Geula","year":"2015","journal-title":"Science"},{"key":"10.1016\/j.molcel.2024.07.017_bib18","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1016\/j.cell.2020.05.012","article-title":"A Unified Model for the Function of YTHDF Proteins in Regulating m6A-Modified mRNA","volume":"181","author":"Zaccara","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib19","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1016\/j.cell.2015.05.014","article-title":"N(6)-methyladenosine Modulates Messenger RNA Translation Efficiency","volume":"161","author":"Wang","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib20","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/nature11112","article-title":"Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq","volume":"485","author":"Dominissini","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2024.07.017_bib21","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1016\/j.cell.2012.05.003","article-title":"Comprehensive analysis of mRNA methylation reveals enrichment in 3\u2032 UTRs and near stop codons","volume":"149","author":"Meyer","year":"2012","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib22","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.celrep.2014.05.048","article-title":"Perturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5\u2032 sites","volume":"8","author":"Schwartz","year":"2014","journal-title":"Cell Rep."},{"key":"10.1016\/j.molcel.2024.07.017_bib23","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1101\/gad.269415.115","article-title":"A majority of m6A residues are in the last exons, allowing the potential for 3\u2032 UTR regulation","volume":"29","author":"Ke","year":"2015","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2024.07.017_bib24","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1038\/nmeth.3898","article-title":"m(6)A-LAIC-seq reveals the census and complexity of the m(6)A epitranscriptome","volume":"13","author":"Molinie","year":"2016","journal-title":"Nat. Methods"},{"key":"10.1016\/j.molcel.2024.07.017_bib25","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/nchembio.1432","article-title":"A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation","volume":"10","author":"Liu","year":"2014","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.molcel.2024.07.017_bib26","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1038\/cr.2014.3","article-title":"Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase","volume":"24","author":"Ping","year":"2014","journal-title":"Cell Res."},{"key":"10.1016\/j.molcel.2024.07.017_bib27","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1016\/j.molcel.2018.02.015","article-title":"Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal","volume":"69","author":"Wen","year":"2018","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib28","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1038\/s41580-019-0168-5","article-title":"Reading, writing and erasing mRNA methylation","volume":"20","author":"Zaccara","year":"2019","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.molcel.2024.07.017_bib29","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.18434","article-title":"Structural insights into the molecular mechanism of the m(6)A writer complex","volume":"5","author":"\u015aled\u017a","year":"2016","journal-title":"eLife"},{"key":"10.1016\/j.molcel.2024.07.017_bib30","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.molcel.2016.05.041","article-title":"Structural Basis for Cooperative Function of Mettl3 and Mettl14 Methyltransferases","volume":"63","author":"Wang","year":"2016","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib31","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1038\/nature18298","article-title":"Structural basis of N(6)-adenosine methylation by the METTL3-METTL14 complex","volume":"534","author":"Wang","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2024.07.017_bib32","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1038\/nchembio.687","article-title":"N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO","volume":"7","author":"Jia","year":"2011","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.molcel.2024.07.017_bib33","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/j.stem.2015.09.005","article-title":"Coordination of m(6)A mRNA Methylation and Gene Transcription by ZFP217 Regulates Pluripotency and Reprogramming","volume":"17","author":"Aguilo","year":"2015","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib34","doi-asserted-by":"crossref","first-page":"5195","DOI":"10.1093\/nar\/gky156","article-title":"SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function","volume":"46","author":"Du","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2024.07.017_bib35","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.tcb.2019.02.008","article-title":"Regulation of Gene Expression by N6-methyladenosine in Cancer","volume":"29","author":"Liu","year":"2019","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.molcel.2024.07.017_bib36","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1038\/nature24678","article-title":"Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control","volume":"552","author":"Barbieri","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2024.07.017_bib37","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1038\/nature25784","article-title":"The SMAD2\/3 interactome reveals that TGFbeta controls m6A mRNA methylation in pluripotency","volume":"555","author":"Bertero","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2024.07.017_bib38","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.cell.2017.03.031","article-title":"Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation","volume":"169","author":"Slobodin","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib39","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1038\/s41586-019-1016-7","article-title":"Histone H3 trimethylation at lysine 36 guides m6A RNA modification co-transcriptionally","volume":"567","author":"Huang","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2024.07.017_bib40","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/s41594-021-00709-z","article-title":"Alternative polyadenylation by sequential activation of distal and proximal PolyA sites","volume":"29","author":"Tang","year":"2022","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.molcel.2024.07.017_bib41","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1016\/j.molcel.2019.11.017","article-title":"Splicing Kinetics and Coordination Revealed by Direct Nascent RNA Sequencing through Nanopores","volume":"77","author":"Drexler","year":"2020","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib42","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.1016\/j.molcel.2021.02.034","article-title":"POINT technology illuminates the processing of polymerase-associated intact nascent transcripts","volume":"81","author":"Sousa-Lu\u00eds","year":"2021","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib43","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1038\/nmeth.4435","article-title":"Thiol-linked alkylation of RNA to assess expression dynamics","volume":"14","author":"Herzog","year":"2017","journal-title":"Nat. Methods"},{"key":"10.1016\/j.molcel.2024.07.017_bib44","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1146\/annurev-genom-090413-025405","article-title":"The pivotal regulatory landscape of RNA modifications","volume":"15","author":"Li","year":"2014","journal-title":"Annu. Rev. Genomics Hum. Genet."},{"key":"10.1016\/j.molcel.2024.07.017_bib45","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-63355-3","article-title":"Limits in the detection of m6A changes using MeRIP\/m6A-seq","volume":"10","author":"McIntyre","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.molcel.2024.07.017_bib46","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1038\/s41587-022-01487-9","article-title":"Absolute quantification of single-base m6A methylation in the mammalian transcriptome using GLORI","volume":"41","author":"Liu","year":"2023","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.molcel.2024.07.017_bib47","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.molcel.2015.07.023","article-title":"Tracking Distinct RNA Populations Using Efficient and Reversible Covalent Chemistry","volume":"59","author":"Duffy","year":"2015","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib48","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/s13059-017-1184-4","article-title":"IRFinder: assessing the impact of intron retention on mammalian gene expression","volume":"18","author":"Middleton","year":"2017","journal-title":"Genome Biol."},{"key":"10.1016\/j.molcel.2024.07.017_bib49","doi-asserted-by":"crossref","first-page":"3429","DOI":"10.1016\/j.celrep.2018.05.077","article-title":"Transient N-6-Methyladenosine Transcriptome Sequencing Reveals a Regulatory Role of m6A in Splicing Efficiency","volume":"23","author":"Louloupi","year":"2018","journal-title":"Cell Rep."},{"key":"10.1016\/j.molcel.2024.07.017_bib50","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1101\/gr.132159.111","article-title":"The GENCODE v7 catalog of human long noncoding RNAs: analysis of their gene structure, evolution, and expression","volume":"22","author":"Derrien","year":"2012","journal-title":"Genome Res."},{"key":"10.1016\/j.molcel.2024.07.017_bib51","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1101\/gad.302604.117","article-title":"An Mtr4\/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression","volume":"31","author":"Ogami","year":"2017","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2024.07.017_bib52","doi-asserted-by":"crossref","first-page":"3386","DOI":"10.1073\/pnas.0800250105","article-title":"Splicing promotes rapid and efficient mRNA export in mammalian cells","volume":"105","author":"Valencia","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.molcel.2024.07.017_bib53","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.molcel.2022.12.026","article-title":"Exclusion of m6A from splice-site proximal regions by the exon junction complex dictates m6A topologies and mRNA stability","volume":"83","author":"Uzonyi","year":"2023","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib54","doi-asserted-by":"crossref","first-page":"7904","DOI":"10.1038\/s41467-022-35643-1","article-title":"Exon junction complex shapes the m6A epitranscriptome","volume":"13","author":"Yang","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.molcel.2024.07.017_bib55","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1126\/science.abj9090","article-title":"Exon architecture controls mRNA m6A suppression and gene expression","volume":"379","author":"He","year":"2023","journal-title":"Science"},{"key":"10.1016\/j.molcel.2024.07.017_bib56","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/s41556-018-0045-z","article-title":"Recognition of RNA N6-methyladenosine by IGF2BP proteins enhances mRNA stability and translation","volume":"20","author":"Huang","year":"2018","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.molcel.2024.07.017_bib57","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms2005","article-title":"TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export","volume":"3","author":"Viphakone","year":"2012","journal-title":"Nat. Commun."},{"key":"10.1016\/j.molcel.2024.07.017_bib58","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-020-19532-z","article-title":"The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice","volume":"11","author":"Yesbolatova","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.molcel.2024.07.017_bib59","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1101\/gr.237826.118","article-title":"A compendium of conserved cleavage and polyadenylation events in mammalian genes","volume":"28","author":"Wang","year":"2018","journal-title":"Genome Res."},{"key":"10.1016\/j.molcel.2024.07.017_bib60","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1101\/gad.3.7.1019","article-title":"Definition of an efficient synthetic poly(A) site","volume":"3","author":"Levitt","year":"1989","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2024.07.017_bib61","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/0092-8674(87)90292-3","article-title":"Position-dependent sequence elements downstream of AAUAAA are required for efficient rabbit \u03b2-globin mRNA 3\u2032 end formation","volume":"49","author":"Gil","year":"1987","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib62","doi-asserted-by":"crossref","first-page":"3899","DOI":"10.1093\/nar\/17.10.3899","article-title":"Functional analysis of point mutations in the AAUAAA motif of the SV40 late polyadenylation signal","volume":"17","author":"Wilusz","year":"1989","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2024.07.017_bib63","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/s41583-019-0244-z","article-title":"The m6A epitranscriptome: transcriptome plasticity in brain development and function","volume":"21","author":"Livneh","year":"2020","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1016\/j.molcel.2024.07.017_bib64","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.tcb.2011.03.003","article-title":"Pre-mRNA splicing: where and when in the nucleus","volume":"21","author":"Han","year":"2011","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.molcel.2024.07.017_bib65","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms1998","article-title":"Post-transcriptional spliceosomes are retained in nuclear speckles until splicing completion","volume":"3","author":"Girard","year":"2012","journal-title":"Nat. Commun."},{"key":"10.1016\/j.molcel.2024.07.017_bib66","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.cell.2012.10.007","article-title":"The cellular EJC interactome reveals higher-order mRNP structure and an EJC-SR protein nexus","volume":"151","author":"Singh","year":"2012","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib67","doi-asserted-by":"crossref","first-page":"3125","DOI":"10.1016\/j.cell.2021.03.062","article-title":"Splice site m6A methylation prevents binding of U2AF35 to inhibit RNA splicing","volume":"184","author":"Mendel","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib68","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.molcel.2019.07.005","article-title":"Regulation of Co-transcriptional Pre-mRNA Splicing by m6A through the Low-Complexity Protein hnRNPG","volume":"76","author":"Zhou","year":"2019","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib69","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1002\/cpch.12","article-title":"Enriching s4U-RNA Using Methane Thiosulfonate (MTS) Chemistry","volume":"8","author":"Duffy","year":"2016","journal-title":"Curr. Protoc. Chem. Biol."},{"key":"10.1016\/j.molcel.2024.07.017_bib70","doi-asserted-by":"crossref","DOI":"10.1002\/imt2.107","article-title":"Ultrafast one-pass FASTQ data preprocessing, quality control, and deduplication using fastp","volume":"2","author":"Chen","year":"2023","journal-title":"Imeta"},{"key":"10.1016\/j.molcel.2024.07.017_bib71","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.molcel.2024.07.017_bib72","doi-asserted-by":"crossref","DOI":"10.1093\/gigascience\/giab008","article-title":"Twelve years of SAMtools and BCFtools","volume":"10","author":"Danecek","year":"2021","journal-title":"GigaScience"},{"key":"10.1016\/j.molcel.2024.07.017_bib73","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet.journal"},{"key":"10.1016\/j.molcel.2024.07.017_bib74","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1186\/s12859-018-2579-2","article-title":"NGmerge: merging paired-end reads via novel empirically-derived models of sequencing errors","volume":"19","author":"Gaspar","year":"2018","journal-title":"BMC Bioinformatics"},{"key":"10.1016\/j.molcel.2024.07.017_bib75","doi-asserted-by":"crossref","first-page":"4494","DOI":"10.1016\/j.molcel.2023.10.040","article-title":"m(6)A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3","volume":"83","author":"Shan","year":"2023","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2024.07.017_bib76","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1038\/nmeth.2483","article-title":"Comparative analysis of RNA sequencing methods for degraded or low-input samples","volume":"10","author":"Adiconis","year":"2013","journal-title":"Nat. Methods"},{"key":"10.1016\/j.molcel.2024.07.017_bib77","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1101\/gr.202432.115","article-title":"A comprehensive analysis of 3\u2032 end sequencing data sets reveals novel polyadenylation signals and the repressive role of heterogeneous ribonucleoprotein C on cleavage and polyadenylation","volume":"26","author":"Gruber","year":"2016","journal-title":"Genome Res."}],"container-title":["Molecular Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1097276524005938?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1097276524005938?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T20:59:11Z","timestamp":1759611551000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1097276524005938"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":77,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["S1097276524005938"],"URL":"https:\/\/doi.org\/10.1016\/j.molcel.2024.07.017","relation":{},"ISSN":["1097-2765"],"issn-type":[{"value":"1097-2765","type":"print"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Nuclear retention coupled with sequential polyadenylation dictates post-transcriptional m6A modification in the nucleus","name":"articletitle","label":"Article Title"},{"value":"Molecular Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.molcel.2024.07.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier Inc.","name":"copyright","label":"Copyright"}]}}