{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:57:01Z","timestamp":1792205821708,"version":"4.4.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"22","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["22S11900200"],"award-info":[{"award-number":["22S11900200"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003347","name":"Fudan University","doi-asserted-by":"publisher","award":["yg2023-06"],"award-info":[{"award-number":["yg2023-06"]}],"id":[{"id":"10.13039\/501100003347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003347","name":"Fudan University","doi-asserted-by":"publisher","award":["yg2023-26"],"award-info":[{"award-number":["yg2023-26"]}],"id":[{"id":"10.13039\/501100003347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003347","name":"Fudan University","doi-asserted-by":"publisher","award":["2021318"],"award-info":[{"award-number":["2021318"]}],"id":[{"id":"10.13039\/501100003347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013285","name":"Program for Professor of Special Appointment Eastern Scholar at Shanghai Institutions of Higher Learning","doi-asserted-by":"publisher","award":["TP2022046"],"award-info":[{"award-number":["TP2022046"]}],"id":[{"id":"10.13039\/501100013285","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82301215"],"award-info":[{"award-number":["82301215"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271048"],"award-info":[{"award-number":["82271048"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Cell"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.molcel.2025.09.031","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T02:29:02Z","timestamp":1760063342000},"page":"4138-4151.e4","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"title":["Engineered hypercompact Fanzor-\u03c9RNA system with enhanced genome editing activity"],"prefix":"10.1016","volume":"85","author":[{"given":"Guoli","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganggang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhilin","family":"Zou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuoyu","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingyi","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyu","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongxing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhai","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingtao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhai","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.molcel.2025.09.031_bib1","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1126\/science.1225829","article-title":"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity","volume":"337","author":"Jinek","year":"2012","journal-title":"Science"},{"key":"10.1016\/j.molcel.2025.09.031_bib2","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1126\/science.1232033","article-title":"RNA-guided human genome engineering via Cas9","volume":"339","author":"Mali","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.molcel.2025.09.031_bib3","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1126\/science.1231143","article-title":"Multiplex genome engineering using CRISPR\/Cas systems","volume":"339","author":"Cong","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.molcel.2025.09.031_bib4","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.cell.2015.09.038","article-title":"Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system","volume":"163","author":"Zetsche","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2025.09.031_bib5","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1038\/s41587-018-0011-0","article-title":"Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing","volume":"37","author":"Kleinstiver","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.molcel.2025.09.031_bib6","doi-asserted-by":"crossref","first-page":"3908","DOI":"10.1038\/s41467-021-24017-8","article-title":"AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines","volume":"12","author":"Zhang","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.molcel.2025.09.031_bib7","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1038\/nmeth.3993","article-title":"A multifunctional AAV-CRISPR-Cas9 and its host response","volume":"13","author":"Chew","year":"2016","journal-title":"Nat. Methods"},{"key":"10.1016\/j.molcel.2025.09.031_bib8","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.molcel.2020.01.016","article-title":"Multiple Input Sensing and Signal Integration Using a Split Cas12a System","volume":"78","author":"Kempton","year":"2020","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2025.09.031_bib9","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1038\/nbt.3469","article-title":"A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice","volume":"34","author":"Yang","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.molcel.2025.09.031_bib10","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1038\/s41586-021-04058-1","article-title":"Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease","volume":"599","author":"Karvelis","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2025.09.031_bib11","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1126\/science.abq7220","article-title":"Structural basis for RNA-guided DNA cleavage by IscB-\u03c9RNA and mechanistic comparison with Cas9","volume":"376","author":"Schuler","year":"2022","journal-title":"Science"},{"key":"10.1016\/j.molcel.2025.09.031_bib12","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1016\/j.tibtech.2023.08.003","article-title":"Fanzor: a compact programmable RNA-guided endonuclease from eukaryotes","volume":"41","author":"Awan","year":"2023","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.molcel.2025.09.031_bib13","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1038\/s41586-023-06356-2","article-title":"Fanzor is a eukaryotic programmable RNA-guided endonuclease","volume":"620","author":"Saito","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2025.09.031_bib14","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adk0171","article-title":"Programmable RNA-guided DNA endonucleases are widespread in eukaryotes and their viruses","volume":"9","author":"Jiang","year":"2023","journal-title":"Sci. Adv."},{"key":"10.1016\/j.molcel.2025.09.031_bib15","doi-asserted-by":"crossref","first-page":"5238","DOI":"10.1016\/j.cell.2024.07.050","article-title":"Structural insights into the diversity and DNA cleavage mechanism of Fanzor","volume":"187","author":"Xu","year":"2024","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2025.09.031_bib16","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1007\/s12010-016-2122-8","article-title":"Highly Efficient and Rapid Detection of the Cleavage Activity of Cas9\/gRNA via a Fluorescent Reporter","volume":"180","author":"Yang","year":"2016","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.molcel.2025.09.031_bib17","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1038\/s41587-020-0561-9","article-title":"Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors","volume":"38","author":"Anzalone","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.molcel.2025.09.031_bib18","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-17081-y","article-title":"Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains","volume":"7","author":"Spencer","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.molcel.2025.09.031_bib19","first-page":"538","article-title":"An engineered xCas12i with high activity, high specificity, and broad PAM range","volume":"14","author":"Zhang","year":"2023","journal-title":"Protein Cell"},{"key":"10.1016\/j.molcel.2025.09.031_bib20","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1038\/s41589-023-01380-9","article-title":"An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity","volume":"19","author":"Wu","year":"2023","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.molcel.2025.09.031_bib21","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1038\/s41592-023-01898-9","article-title":"Development of miniature base editors using engineered IscB nickase","volume":"20","author":"Han","year":"2023","journal-title":"Nat. Methods"},{"key":"10.1016\/j.molcel.2025.09.031_bib22","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1038\/s41467-023-37829-7","article-title":"Engineered CRISPR-OsCas12f1 and RhCas12f1 with robust activities and expanded target range for genome editing","volume":"14","author":"Kong","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.molcel.2025.09.031_bib23","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1089\/crispr.2020.0073","article-title":"Improving FnCas12a Genome Editing by Exonuclease Fusion","volume":"3","author":"Wu","year":"2020","journal-title":"CRISPR J."},{"key":"10.1016\/j.molcel.2025.09.031_bib24","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1038\/s41467-022-28900-w","article-title":"Cas9 exo-endonuclease eliminates chromosomal translocations during genome editing","volume":"13","author":"Yin","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.molcel.2025.09.031_bib25","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1038\/nbt.4102","article-title":"Base editing with a Cpf1-cytidine deaminase fusion","volume":"36","author":"Li","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.molcel.2025.09.031_bib26","doi-asserted-by":"crossref","first-page":"6240","DOI":"10.3390\/ijms21176240","article-title":"CRISPR-Cas9 DNA Base-Editing and Prime-Editing","volume":"21","author":"Kantor","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.molcel.2025.09.031_bib27","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1038\/s41587-020-0453-z","article-title":"Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity","volume":"38","author":"Richter","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.molcel.2025.09.031_bib28","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1038\/s41592-024-02418-z","article-title":"Effective genome editing with an enhanced ISDra2 TnpB system and deep learning-predicted \u03c9RNAs","volume":"21","author":"Marquart","year":"2024","journal-title":"Nat. Methods"},{"key":"10.1016\/j.molcel.2025.09.031_bib29","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/s41587-021-01009-z","article-title":"Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus","volume":"40","author":"Kim","year":"2022","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.molcel.2025.09.031_bib30","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1038\/s41467-024-45197-z","article-title":"Engineering a transposon-associated TnpB-\u03c9RNA system for efficient gene editing and phenotypic correction of a tyrosinaemia mouse model","volume":"15","author":"Li","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.molcel.2025.09.031_bib31","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1093\/bioinformatics\/btu048","article-title":"Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases","volume":"30","author":"Bae","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.molcel.2025.09.031_bib32","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1038\/s41589-024-01669-3","article-title":"Assessing and engineering the IscB-\u03c9RNA system for programmed genome editing","volume":"20","author":"Yan","year":"2024","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.molcel.2025.09.031_bib33","doi-asserted-by":"crossref","first-page":"3128","DOI":"10.1016\/j.molcel.2024.07.007","article-title":"Engineering IscB to develop highly efficient miniature editing tools in mammalian cells and embryos","volume":"84","author":"Xue","year":"2024","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2025.09.031_bib34","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1038\/nbt.3404","article-title":"Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition","volume":"33","author":"Kleinstiver","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.molcel.2025.09.031_bib35","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1038\/nbt.3526","article-title":"Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow","volume":"34","author":"Ma","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.molcel.2025.09.031_bib36","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/nature17946","article-title":"Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage","volume":"533","author":"Komor","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2025.09.031_bib37","article-title":"Engineering eukaryotic transposon-encoded Fanzor2 system for genome editing in mammals","author":"Wei","year":"2025","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.molcel.2025.09.031_bib38","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1038\/s41587-019-0032-3","article-title":"CRISPResso2 provides accurate and rapid genome editing sequence analysis","volume":"37","author":"Clement","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.molcel.2025.09.031_bib39","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1038\/nbt.3534","article-title":"Open-source guideseq software for analysis of GUIDE-seq data","volume":"34","author":"Tsai","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.molcel.2025.09.031_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2021.101088","article-title":"PEM-seq comprehensively quantifies DNA repair outcomes during gene-editing and DSB repair","volume":"3","author":"Liu","year":"2022","journal-title":"STAR Protoc."}],"container-title":["Molecular Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1097276525008160?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1097276525008160?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T20:30:42Z","timestamp":1786998642000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1097276525008160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":40,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["S1097276525008160"],"URL":"https:\/\/doi.org\/10.1016\/j.molcel.2025.09.031","relation":{},"ISSN":["1097-2765"],"issn-type":[{"value":"1097-2765","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Engineered hypercompact Fanzor-\u03c9RNA system with enhanced genome editing activity","name":"articletitle","label":"Article Title"},{"value":"Molecular Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.molcel.2025.09.031","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}