{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T02:42:58Z","timestamp":1778726578518,"version":"3.51.4"},"reference-count":110,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,4,2]],"date-time":"2027-04-02T00:00:00Z","timestamp":1806624000000},"content-version":"am","delay-in-days":366,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Cell"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1016\/j.molcel.2026.03.003","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T14:48:20Z","timestamp":1775141300000},"page":"1345-1361.e9","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transient residence of the repulsive client Shutdown in Yb bodies plays a critical role in Piwi-piRISC biogenesis and maintaining fertility"],"prefix":"10.1016","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6637-7496","authenticated-orcid":false,"given":"Shigeki","family":"Hirakata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Fukaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aoi","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidetaka","family":"Kosako","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6149-4396","authenticated-orcid":false,"given":"Mikiko C.","family":"Siomi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.molcel.2026.03.003_bib1","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/nrm.2017.7","article-title":"Biomolecular condensates: organizers of cellular biochemistry","volume":"18","author":"Banani","year":"2017","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.molcel.2026.03.003_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12964-015-0125-7","article-title":"Phase separation in biology; functional organization of a higher order","volume":"14","author":"Mitrea","year":"2016","journal-title":"Cell Commun. Signal."},{"key":"10.1016\/j.molcel.2026.03.003_bib3","doi-asserted-by":"crossref","DOI":"10.1126\/science.aaf4382","article-title":"Liquid phase condensation in cell physiology and disease","volume":"357","author":"Shin","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.molcel.2026.03.003_bib4","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.tcb.2018.02.004","article-title":"Protein Phase Separation: A New Phase in Cell Biology","volume":"28","author":"Boeynaems","year":"2018","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.molcel.2026.03.003_bib5","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1016\/j.molcel.2022.05.018","article-title":"A conceptual framework for understanding phase separation and addressing open questions and challenges","volume":"82","author":"Mittag","year":"2022","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2026.03.003_bib6","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/j.cell.2016.06.010","article-title":"Compositional Control of Phase-Separated Cellular Bodies","volume":"166","author":"Banani","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2026.03.003_bib7","doi-asserted-by":"crossref","first-page":"14823","DOI":"10.1074\/jbc.REV119.007895","article-title":"Formation of biological condensates via phase separation: Characteristics, analytical methods, and physiological implications","volume":"294","author":"Feng","year":"2019","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.molcel.2026.03.003_bib8","doi-asserted-by":"crossref","first-page":"55","DOI":"10.3389\/fgene.2011.00055","article-title":"Gender-Specific Hierarchy in Nuage Localization of PIWI-Interacting RNA Factors in Drosophila","volume":"2","author":"Nagao","year":"2011","journal-title":"Front. Genet."},{"key":"10.1016\/j.molcel.2026.03.003_bib9","doi-asserted-by":"crossref","DOI":"10.15252\/embr.201947708","article-title":"Requirements for multivalent Yb body assembly in transposon silencing in Drosophila","volume":"20","author":"Hirakata","year":"2019","journal-title":"EMBO Rep."},{"key":"10.1016\/j.molcel.2026.03.003_bib10","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-022-11177-w","article-title":"Structure-dependent recruitment and diffusion of guest proteins in liquid droplets of FUS","volume":"12","author":"Kamagata","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.molcel.2026.03.003_bib11","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.cell.2020.03.049","article-title":"RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation","volume":"181","author":"Guill\u00e9n-Boixet","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2026.03.003_bib12","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/s41556-020-00606-5","article-title":"Phase separation of SERRATE drives dicing body assembly and promotes miRNA processing in Arabidopsis","volume":"23","author":"Xie","year":"2021","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.molcel.2026.03.003_bib13","doi-asserted-by":"crossref","DOI":"10.15252\/embr.201948769","article-title":"Armitage determines Piwi\u2013piRISC processing from precursor formation and quality control to inter-organelle translocation","volume":"21","author":"Yamashiro","year":"2020","journal-title":"EMBO Rep."},{"key":"10.1016\/j.molcel.2026.03.003_bib14","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/s41586-020-2256-2","article-title":"Composition-dependent thermodynamics of intracellular phase separation","volume":"581","author":"Riback","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2026.03.003_bib15","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1146\/annurev-genet-120417-031441","article-title":"piRNA-Guided Genome Defense: From Biogenesis to Silencing","volume":"52","author":"Czech","year":"2018","journal-title":"Annu. Rev. Genet."},{"key":"10.1016\/j.molcel.2026.03.003_bib16","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1016\/j.tig.2017.09.002","article-title":"piRNA Biogenesis in Drosophila melanogaster","volume":"33","author":"Huang","year":"2017","journal-title":"Trends Genet."},{"key":"10.1016\/j.molcel.2026.03.003_bib17","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/s41576-018-0073-3","article-title":"PIWI-interacting RNAs: small RNAs with big functions","volume":"20","author":"Ozata","year":"2019","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.molcel.2026.03.003_bib18","doi-asserted-by":"crossref","DOI":"10.1098\/rsob.180181","article-title":"Rapid evolution and conserved function of the piRNA pathway","volume":"9","author":"Parhad","year":"2019","journal-title":"Open Biol."},{"key":"10.1016\/j.molcel.2026.03.003_bib19","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s13059-020-02221-x","article-title":"Roles of piRNAs in transposon and pseudogene regulation of germline mRNAs and lncRNAs","volume":"22","author":"Wang","year":"2021","journal-title":"Genome Biol."},{"key":"10.1016\/j.molcel.2026.03.003_bib20","doi-asserted-by":"crossref","first-page":"5335","DOI":"10.1038\/s44318-024-00253-8","article-title":"PIWI-interacting RNAs: who, what, when, where, why, and how","volume":"43","author":"Haase","year":"2024","journal-title":"EMBO J."},{"key":"10.1016\/j.molcel.2026.03.003_bib21","doi-asserted-by":"crossref","first-page":"2493","DOI":"10.1101\/gad.1989510","article-title":"Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila","volume":"24","author":"Saito","year":"2010","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2026.03.003_bib22","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1083\/jcb.200903034","article-title":"The Yb protein defines a novel organelle and regulates male germline stem cell self-renewal in Drosophila melanogaster","volume":"185","author":"Szakmary","year":"2009","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.molcel.2026.03.003_bib23","doi-asserted-by":"crossref","first-page":"3301","DOI":"10.1038\/emboj.2010.212","article-title":"An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila","volume":"29","author":"Olivieri","year":"2010","journal-title":"EMBO J."},{"key":"10.1016\/j.molcel.2026.03.003_bib24","doi-asserted-by":"crossref","first-page":"3789","DOI":"10.1074\/jbc.M110.193888","article-title":"The Yb Body, a Major Site for Piwi-associated RNA Biogenesis and a Gateway for Piwi Expression and Transport to the Nucleus in Somatic Cells","volume":"286","author":"Qi","year":"2011","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.molcel.2026.03.003_bib25","first-page":"52","article-title":"Assembly and function of gonad-specific non-membranous organelles in Drosophila piRNA biogenesis","volume":"5","author":"Hirakata","year":"2019","journal-title":"Noncoding. RNA"},{"key":"10.1016\/j.molcel.2026.03.003_bib26","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1093\/genetics\/88.4.723","article-title":"Nonessential Sequences, Genes, and the Polytene Chromosome Bands of DROSOPHILA MELANOGASTER","volume":"88","author":"Young","year":"1978","journal-title":"Genetics"},{"key":"10.1016\/j.molcel.2026.03.003_bib27","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1016\/j.celrep.2019.04.029","article-title":"Distinct and Collaborative Functions of Yb and Armitage in Transposon-Targeting piRNA Biogenesis","volume":"27","author":"Ishizu","year":"2019","journal-title":"Cell Rep."},{"key":"10.1016\/j.molcel.2026.03.003_bib28","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1038\/nature11509","article-title":"Structure and function of Zucchini endoribonuclease in piRNA biogenesis","volume":"491","author":"Nishimasu","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2026.03.003_bib29","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/nature11502","article-title":"The structural biochemistry of Zucchini implicates it as a nuclease in piRNA biogenesis","volume":"491","author":"Ipsaro","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2026.03.003_bib30","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1101\/gad.325662.119","article-title":"Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery","volume":"33","author":"Munaf\u00f2","year":"2019","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2026.03.003_bib31","doi-asserted-by":"crossref","first-page":"3977","DOI":"10.1038\/emboj.2011.308","article-title":"A systematic analysis of Drosophila TUDOR domain-containing proteins identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway factors","volume":"30","author":"Handler","year":"2011","journal-title":"EMBO J."},{"key":"10.1016\/j.molcel.2026.03.003_bib32","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1016\/j.molcel.2012.07.021","article-title":"The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila","volume":"47","author":"Olivieri","year":"2012","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2026.03.003_bib33","doi-asserted-by":"crossref","first-page":"4039","DOI":"10.1242\/dev.069187","article-title":"Vreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila","volume":"138","author":"Zamparini","year":"2011","journal-title":"Development"},{"key":"10.1016\/j.molcel.2026.03.003_bib34","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.1261\/rna.034405.112","article-title":"shutdown is a component of the Drosophila piRNA biogenesis machinery","volume":"18","author":"Preall","year":"2012","journal-title":"RNA"},{"key":"10.1016\/j.molcel.2026.03.003_bib35","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1093\/genetics\/156.1.245","article-title":"The shut-down gene of Drosophila melanogaster encodes a novel FK506-binding protein essential for the formation of germline cysts during oogenesis","volume":"156","author":"Munn","year":"2000","journal-title":"Genetics"},{"key":"10.1016\/j.molcel.2026.03.003_bib36","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.1021\/bi00461a001","article-title":"The mechanism of protein folding. Implications of in vitro refolding models for de novo protein folding and translocation in the cell","volume":"29","author":"Fischer","year":"1990","journal-title":"Biochemistry"},{"key":"10.1016\/j.molcel.2026.03.003_bib37","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0968-0004(00)89037-4","article-title":"Tetratrico peptide repeat interactions: to TPR or not to TPR?","volume":"20","author":"Lamb","year":"1995","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.molcel.2026.03.003_bib38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11103-009-9547-1","article-title":"Genome-wide analysis of genes encoding FK506-binding proteins in rice","volume":"72","author":"Gollan","year":"2010","journal-title":"Plant Mol. Biol."},{"key":"10.1016\/j.molcel.2026.03.003_bib39","doi-asserted-by":"crossref","first-page":"420","DOI":"10.3181\/00379727-217-44252","article-title":"The hsp90-based chaperone system: involvement in signal transduction from a variety of hormone and growth factor receptors","volume":"217","author":"Pratt","year":"1998","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"10.1016\/j.molcel.2026.03.003_bib40","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s12192-010-0248-0","article-title":"Versatile TPR domains accommodate different modes of target protein recognition and function","volume":"16","author":"Allan","year":"2011","journal-title":"Cell Stress Chaperones"},{"key":"10.1016\/j.molcel.2026.03.003_bib41","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1038\/nature08501","article-title":"A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila","volume":"461","author":"Saito","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2026.03.003_bib42","doi-asserted-by":"crossref","first-page":"3647","DOI":"10.1016\/j.celrep.2018.05.051","article-title":"Piwi Nuclear Localization and Its Regulatory Mechanism in Drosophila Ovarian Somatic Cells","volume":"23","author":"Yashiro","year":"2018","journal-title":"Cell Rep."},{"key":"10.1016\/j.molcel.2026.03.003_bib43","doi-asserted-by":"crossref","DOI":"10.1126\/science.adq8381","article-title":"Sequence-based prediction of intermolecular interactions driven by disordered regions","volume":"388","author":"Ginell","year":"2025","journal-title":"Science"},{"key":"10.1016\/j.molcel.2026.03.003_bib44","doi-asserted-by":"crossref","first-page":"4312","DOI":"10.1016\/j.bpj.2021.08.039","article-title":"Metapredict: a fast, accurate, and easy-to-use predictor of consensus disorder and structure","volume":"120","author":"Emenecker","year":"2021","journal-title":"Biophys. J."},{"key":"10.1016\/j.molcel.2026.03.003_bib45","doi-asserted-by":"crossref","first-page":"8203","DOI":"10.1021\/acs.jpcb.9b06808","article-title":"Calculation of Second Virial Coefficients of Atomistic Proteins Using Fast Fourier Transform","volume":"123","author":"Qin","year":"2019","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.molcel.2026.03.003_bib46","doi-asserted-by":"crossref","first-page":"2657","DOI":"10.1016\/j.bpj.2010.08.020","article-title":"Diffusion and Sedimentation Interaction Parameters for Measuring the Second Virial Coefficient and Their Utility as Predictors of Protein Aggregation","volume":"99","author":"Saluja","year":"2010","journal-title":"Biophys. J."},{"key":"10.1016\/j.molcel.2026.03.003_bib47","doi-asserted-by":"crossref","first-page":"4812","DOI":"10.1002\/jps.22190","article-title":"Factors affecting the viscosity in high concentration solutions of different monoclonal antibodies","volume":"99","author":"Yadav","year":"2010","journal-title":"J. Pharm. Sci."},{"key":"10.1016\/j.molcel.2026.03.003_bib48","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/j.jmb.2005.07.038","article-title":"Identification of a universal VHH framework to graft non-canonical antigen-binding loops of camel single-domain antibodies","volume":"352","author":"Saerens","year":"2005","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.molcel.2026.03.003_bib49","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2024.114923","article-title":"Dipteran-specific Daedalus controls Zucchini endonucleolysis in piRNA biogenesis independent of exonucleases","volume":"43","author":"Koga","year":"2024","journal-title":"Cell Rep."},{"key":"10.1016\/j.molcel.2026.03.003_bib50","first-page":"1675","article-title":"Defective piRNA Processing and Azoospermia","volume":"386","author":"Li","year":"2022","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.molcel.2026.03.003_bib51","doi-asserted-by":"crossref","first-page":"11841","DOI":"10.1073\/pnas.1003953107","article-title":"Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway","volume":"107","author":"Zheng","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.molcel.2026.03.003_bib52","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1101\/gad.254631.114","article-title":"The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing","volume":"29","author":"Vourekas","year":"2015","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2026.03.003_bib53","doi-asserted-by":"crossref","DOI":"10.1002\/wrna.1682","article-title":"Unwinding the roles of RNA helicase MOV10","volume":"13","author":"Nawaz","year":"2022","journal-title":"Wiley Interdiscip. Rev. RNA"},{"key":"10.1016\/j.molcel.2026.03.003_bib55","doi-asserted-by":"crossref","first-page":"D439","DOI":"10.1093\/nar\/gkab1061","article-title":"AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models","volume":"50","author":"Varadi","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2026.03.003_bib54","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1186\/1742-4690-9-112","article-title":"Host restriction factors in retroviral infection: promises in virus-host interaction","volume":"9","author":"Zheng","year":"2012","journal-title":"Retrovirology"},{"key":"10.1016\/j.molcel.2026.03.003_bib56","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2026.03.003_bib57","doi-asserted-by":"crossref","first-page":"W122","DOI":"10.1093\/nar\/gkad472","article-title":"DDMut: predicting effects of mutations on protein stability using deep learning","volume":"51","author":"Zhou","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2026.03.003_bib58","doi-asserted-by":"crossref","first-page":"6306","DOI":"10.1074\/jbc.RA118.007222","article-title":"The role of liquid-liquid phase separation in aggregation of the TDP-43 low-complexity domain","volume":"294","author":"Babinchak","year":"2019","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.molcel.2026.03.003_bib59","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2023.113375","article-title":"Ion binding with charge inversion combined with screening modulates DEAD box helicase phase transitions","volume":"42","author":"Crabtree","year":"2023","journal-title":"Cell Rep."},{"key":"10.1016\/j.molcel.2026.03.003_bib60","doi-asserted-by":"crossref","DOI":"10.1103\/PhysRevLett.101.148101","article-title":"Reentrant condensation of proteins in solution induced by multivalent counterions","volume":"101","author":"Zhang","year":"2008","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.molcel.2026.03.003_bib61","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1016\/j.molcel.2012.07.019","article-title":"A role for Fkbp6 and the chaperone machinery in piRNA amplification and transposon silencing","volume":"47","author":"Xiol","year":"2012","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2026.03.003_bib62","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1016\/j.ajhg.2022.09.002","article-title":"The piRNA-pathway factor FKBP6 is essential for spermatogenesis but dispensable for control of meiotic LINE-1 expression in humans","volume":"109","author":"Wyrwoll","year":"2022","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.molcel.2026.03.003_bib63","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1038\/nature10672","article-title":"Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing","volume":"480","author":"Reuter","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2026.03.003_bib64","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-017-00854-4","article-title":"PNLDC1 is essential for piRNA 3\u2032 end trimming and transposon silencing during spermatogenesis in mice","volume":"8","author":"Ding","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.molcel.2026.03.003_bib65","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1007\/s11427-023-2390-5","article-title":"The PIWI-specific insertion module helps load longer piRNAs for translational activation essential for male fertility","volume":"66","author":"Wang","year":"2023","journal-title":"Sci. China Life Sci."},{"key":"10.1016\/j.molcel.2026.03.003_bib66","doi-asserted-by":"crossref","first-page":"559","DOI":"10.15252\/embj.201490062","article-title":"A Tetrahymena Hsp90 co-chaperone promotes siRNA loading by ATP-dependent and ATP-independent mechanisms","volume":"34","author":"Woehrer","year":"2015","journal-title":"EMBO J."},{"key":"10.1016\/j.molcel.2026.03.003_bib67","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1038\/nsmb.1875","article-title":"A direct role for Hsp90 in pre-RISC formation in Drosophila","volume":"17","author":"Miyoshi","year":"2010","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.molcel.2026.03.003_bib68","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.molcel.2010.05.014","article-title":"In vitro assembly of plant RNA-induced silencing complexes facilitated by molecular chaperone HSP90","volume":"39","author":"Iki","year":"2010","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2026.03.003_bib69","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.molcel.2010.05.015","article-title":"Hsc70\/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes","volume":"39","author":"Iwasaki","year":"2010","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2026.03.003_bib70","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s13072-018-0199-8","article-title":"RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5","volume":"11","author":"Okazaki","year":"2018","journal-title":"Epigenetics Chromatin"},{"key":"10.1016\/j.molcel.2026.03.003_bib71","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.celrep.2014.05.043","article-title":"Yb Integrates piRNA Intermediates and Processing Factors into Perinuclear Bodies to Enhance piRISC Assembly","volume":"8","author":"Murota","year":"2014","journal-title":"Cell Rep."},{"key":"10.1016\/j.molcel.2026.03.003_bib72","doi-asserted-by":"crossref","first-page":"2214","DOI":"10.1101\/gad.1454806","article-title":"Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome","volume":"20","author":"Saito","year":"2006","journal-title":"Genes Dev."},{"key":"10.1016\/j.molcel.2026.03.003_bib73","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1038\/ng1312","article-title":"Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome","volume":"36","author":"Parks","year":"2004","journal-title":"Nat. Genet."},{"key":"10.1016\/j.molcel.2026.03.003_bib74","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1038\/35078107","article-title":"Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells","volume":"411","author":"Elbashir","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2026.03.003_bib75","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1126\/science.1157396","article-title":"Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells","volume":"320","author":"Ghildiyal","year":"2008","journal-title":"Science"},{"key":"10.1016\/j.molcel.2026.03.003_bib76","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1016\/j.cub.2007.06.030","article-title":"The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC","volume":"17","author":"Horwich","year":"2007","journal-title":"Curr. Biol."},{"key":"10.1016\/j.molcel.2026.03.003_bib77","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/j.molcel.2014.03.017","article-title":"MOV10 Is a 5\u2032 to 3\u2032 RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3\u2032 UTRs","volume":"54","author":"Gregersen","year":"2014","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2026.03.003_bib78","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.celrep.2015.06.035","article-title":"Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb","volume":"12","author":"Ishizu","year":"2015","journal-title":"Cell Rep."},{"key":"10.1016\/j.molcel.2026.03.003_bib79","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/s41592-019-0638-x","article-title":"DIA-NN: neural networks and interference correction enable deep proteome coverage in high throughput","volume":"17","author":"Demichev","year":"2020","journal-title":"Nat. Methods"},{"key":"10.1016\/j.molcel.2026.03.003_bib80","doi-asserted-by":"crossref","first-page":"W244","DOI":"10.1093\/nar\/gki408","article-title":"The HHpred interactive server for protein homology detection and structure prediction","volume":"33","author":"S\u00f6ding","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2026.03.003_bib81","doi-asserted-by":"crossref","first-page":"W521","DOI":"10.1093\/nar\/gkae241","article-title":"The EMBL-EBI Job Dispatcher sequence analysis tools framework in 2024","volume":"52","author":"Madeira","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2026.03.003_bib82","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.molcel.2026.03.003_bib83","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1002\/cyto.a.20747","article-title":"Evaluation of intensity-based ratiometric FRET in image cytometry--approaches and a software solution","volume":"75","author":"Roszik","year":"2009","journal-title":"Cytometry A"},{"key":"10.1016\/j.molcel.2026.03.003_bib84","doi-asserted-by":"crossref","first-page":"W467","DOI":"10.1093\/nar\/gky508","article-title":"EasyFRAP-web: a web-based tool for the analysis of fluorescence recovery after photobleaching data","volume":"46","author":"Koulouras","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2026.03.003_bib85","series-title":"R: A Language and Environment for Statistical Computing","year":"2024"},{"key":"10.1016\/j.molcel.2026.03.003_bib86","series-title":"Modern Applied Statistics with S","author":"Venables","year":"2002"},{"key":"10.1016\/j.molcel.2026.03.003_bib87","doi-asserted-by":"crossref","first-page":"2351","DOI":"10.1242\/jcs.168740","article-title":"Architectures of multisubunit complexes revealed by a visible immunoprecipitation assay using fluorescent fusion proteins","volume":"128","author":"Katoh","year":"2015","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.molcel.2026.03.003_bib88","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.molcel.2018.02.029","article-title":"Visualization of Transvection in Living Drosophila Embryos","volume":"70","author":"Lim","year":"2018","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2026.03.003_bib89","doi-asserted-by":"crossref","first-page":"L99","DOI":"10.1529\/biophysj.106.096206","article-title":"Cerulean, Venus, and VenusY67C FRET reference standards","volume":"91","author":"Koushik","year":"2006","journal-title":"Biophys. J."},{"key":"10.1016\/j.molcel.2026.03.003_bib90","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2022.892138","article-title":"mCherry contains a fluorescent protein isoform that interferes with its reporter function","volume":"10","author":"Fages-Lartaud","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.molcel.2026.03.003_bib91","doi-asserted-by":"crossref","first-page":"E2967","DOI":"10.1073\/pnas.1405500111","article-title":"Optimized CRISPR\/Cas tools for efficient germline and somatic genome engineering in Drosophila","volume":"111","author":"Port","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.molcel.2026.03.003_bib92","doi-asserted-by":"crossref","first-page":"8376","DOI":"10.1073\/pnas.1810430115","article-title":"Temporal dynamics of pair-rule stripes in living Drosophila embryos","volume":"115","author":"Lim","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.molcel.2026.03.003_bib93","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1073\/pnas.81.7.1991","article-title":"Genomic sequencing","volume":"81","author":"Church","year":"1984","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.molcel.2026.03.003_bib94","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1126\/science.1134426","article-title":"P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster","volume":"314","author":"Venken","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.molcel.2026.03.003_bib95","doi-asserted-by":"crossref","first-page":"3312","DOI":"10.1073\/pnas.0611511104","article-title":"An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases","volume":"104","author":"Bischof","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.molcel.2026.03.003_bib96","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-60327-019-9_1","article-title":"Transgenesis in Drosophila melanogaster","volume":"561","author":"Ringrose","year":"2009","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.molcel.2026.03.003_bib97","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1534\/genetics.113.160713","article-title":"Highly specific and efficient CRISPR\/Cas9-catalyzed homology-directed repair in Drosophila","volume":"196","author":"Gratz","year":"2014","journal-title":"Genetics"},{"key":"10.1016\/j.molcel.2026.03.003_bib98","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-024-52389-0","article-title":"Mettl1-dependent m7G tRNA modification is essential for maintaining spermatogenesis and fertility in Drosophila melanogaster","volume":"15","author":"Kaneko","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.molcel.2026.03.003_bib99","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1038\/nprot.2008.67","article-title":"Improved northern blot method for enhanced detection of small RNA","volume":"3","author":"Pall","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.molcel.2026.03.003_bib100","doi-asserted-by":"crossref","first-page":"D609","DOI":"10.1093\/nar\/gkae1010","article-title":"UniProt: the Universal Protein Knowledgebase in 2025","volume":"53","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2026.03.003_bib101","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/S0168-9525(00)02024-2","article-title":"EMBOSS: the European Molecular Biology Open Software Suite","volume":"16","author":"Rice","year":"2000","journal-title":"Trends Genet."},{"key":"10.1016\/j.molcel.2026.03.003_bib102","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmb.2023.167995","article-title":"CHARMM-GUI PDB Manipulator: Various PDB Structural Modifications for Biomolecular Modeling and Simulation","volume":"435","author":"Park","year":"2023","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.molcel.2026.03.003_bib103","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/s41592-023-02159-5","article-title":"Direct prediction of intrinsically disordered protein conformational properties from sequence","volume":"21","author":"Lotthammer","year":"2024","journal-title":"Nat. Methods"},{"key":"10.1016\/j.molcel.2026.03.003_bib104","doi-asserted-by":"crossref","first-page":"W320","DOI":"10.1093\/nar\/gku316","article-title":"Deciphering key features in protein structures with the new ENDscript server","volume":"42","author":"Robert","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molcel.2026.03.003_bib105","first-page":"353","article-title":"Cell lines derived from late embryonic stages of Drosophila melanogaster","volume":"27","author":"Schneider","year":"1972","journal-title":"J. Embryol. Exp. Morphol."},{"key":"10.1016\/j.molcel.2026.03.003_bib106","doi-asserted-by":"crossref","first-page":"4067","DOI":"10.1016\/j.bpj.2021.08.007","article-title":"Probing interactions of therapeutic antibodies with serum via second virial coefficient measurements","volume":"120","author":"Larsen","year":"2021","journal-title":"Biophys. J."},{"key":"10.1016\/j.molcel.2026.03.003_bib107","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.molcel.2016.06.008","article-title":"Piwi Modulates Chromatin Accessibility by Regulating Multiple Factors Including Histone H1 to Repress Transposons","volume":"63","author":"Iwasaki","year":"2016","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2026.03.003_bib108","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.molcel.2026.03.003_bib109","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-023-30098-w","article-title":"Pixel-by-pixel autofluorescence corrected FRET in fluorescence microscopy improves accuracy for samples with spatially varied autofluorescence to signal ratio","volume":"13","author":"Rebenku","year":"2023","journal-title":"Sci. Rep."},{"key":"10.1016\/j.molcel.2026.03.003_bib110","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/nmeth.3901","article-title":"The Perseus computational platform for comprehensive analysis of (prote)omics data","volume":"13","author":"Tyanova","year":"2016","journal-title":"Nat. Methods"}],"container-title":["Molecular Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1097276526001590?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1097276526001590?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T04:14:31Z","timestamp":1777608871000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1097276526001590"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":110,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["S1097276526001590"],"URL":"https:\/\/doi.org\/10.1016\/j.molcel.2026.03.003","relation":{},"ISSN":["1097-2765"],"issn-type":[{"value":"1097-2765","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Transient residence of the repulsive client Shutdown in Yb bodies plays a critical role in Piwi-piRISC biogenesis and maintaining fertility","name":"articletitle","label":"Article Title"},{"value":"Molecular Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.molcel.2026.03.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}