{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:12:59Z","timestamp":1759104779705},"reference-count":49,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Immunology"],"published-print":{"date-parts":[[2012,6]]},"DOI":"10.1016\/j.molimm.2012.03.015","type":"journal-article","created":{"date-parts":[[2012,4,7]],"date-time":"2012-04-07T12:51:50Z","timestamp":1333803110000},"page":"188-196","source":"Crossref","is-referenced-by-count":22,"title":["A novel disulfide-stabilized single-chain variable antibody fragment against rabies virus G protein with enhanced in vivo neutralizing potency"],"prefix":"10.1016","volume":"51","author":[{"given":"Ye","family":"Duan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tie-jun","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-lai","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruo-sen","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua-fei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-jia","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang-hui","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-ge","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Kong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.molimm.2012.03.015_bib0005","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1038\/294275a0","article-title":"Structure of the glycoprotein gene in rabies virus","volume":"294","author":"Anilionis","year":"1981","journal-title":"Nature"},{"key":"10.1016\/j.molimm.2012.03.015_bib0010","doi-asserted-by":"crossref","first-page":"9062","DOI":"10.1128\/JVI.79.14.9062-9068.2005","article-title":"Novel human monoclonal antibody combination effectively neutralizing natural rabies virus variants and individual in vitro escape mutants","volume":"79","author":"Bakker","year":"2005","journal-title":"J. Virol."},{"key":"10.1016\/j.molimm.2012.03.015_bib0015","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/0092-8674(91)90532-4","article-title":"Identification of a protein required for disulfide bond formation in vivo","volume":"67","author":"Bardwell","year":"1991","journal-title":"Cell"},{"key":"10.1016\/j.molimm.2012.03.015_bib0020","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0022-1759(87)90187-6","article-title":"Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay","volume":"100","author":"Beatty","year":"1987","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.molimm.2012.03.015_bib0025","doi-asserted-by":"crossref","first-page":"7538","DOI":"10.1073\/pnas.90.16.7538","article-title":"A recombinant immunotoxin containing a disulfide-stabilized Fv fragment","volume":"90","author":"Brinkmann","year":"1993","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.molimm.2012.03.015_bib0030","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/j.micinf.2008.02.008","article-title":"Novel human 3-domain disulfide-stabilized antibody fragment against glycoprotein of rabies virus","volume":"10","author":"Cai","year":"2008","journal-title":"Microbes and Infection"},{"key":"10.1016\/j.molimm.2012.03.015_bib0035","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0022-1759(99)00223-9","article-title":"The development of monoclonal human rabies virus-neutralizing antibodies as a substitute for pooled human immune globulin in the prophylactic treatment of rabies virus exposure","volume":"235","author":"Champion","year":"2000","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.molimm.2012.03.015_bib0040","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1007\/BF01314961","article-title":"The glycoprotein G of rhabdoviruses","volume":"140","author":"Coll","year":"1995","journal-title":"Arch. Virol."},{"key":"10.1016\/j.molimm.2012.03.015_bib0045","first-page":"3969","article-title":"Pharmacokinetics of monoclonal immunoglobulin G1, F(ab\u2032)2, and Fab\u2032 in mice","volume":"46","author":"Covell","year":"1986","journal-title":"Cancer Res."},{"key":"10.1016\/j.molimm.2012.03.015_bib0050","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1146\/annurev.iy.01.040183.000511","article-title":"Structural basis of antibody function","volume":"1","author":"Davies","year":"1983","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.molimm.2012.03.015_bib0055","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1146\/annurev.med.58.061705.145053","article-title":"A human monoclonal antibody cocktail as a novel component of rabies postexposure prophylaxis","volume":"58","author":"de Kruif","year":"2007","journal-title":"Annual Review of Medicine"},{"key":"10.1016\/j.molimm.2012.03.015_bib0060","doi-asserted-by":"crossref","first-page":"3087","DOI":"10.1128\/JVI.64.6.3087-3090.1990","article-title":"Biological characterization of human monoclonal antibodies to rabies virus","volume":"64","author":"Dietzschold","year":"1990","journal-title":"J. Virol."},{"key":"10.1016\/j.molimm.2012.03.015_bib0065","doi-asserted-by":"crossref","DOI":"10.1021\/jp200060q","article-title":"Thermal stability and structural variations of survivin and its deletants in aqueous solution as revealed by spectroscopy","author":"Gao","year":"2011","journal-title":"The Journal of Physical Chemistry B"},{"key":"10.1016\/j.molimm.2012.03.015_bib0070","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1086\/500470","article-title":"Comparison of an anti-rabies human monoclonal antibody combination with human polyclonal anti-rabies immune globulin","volume":"193","author":"Goudsmit","year":"2006","journal-title":"Journal of Infectious Diseases"},{"key":"10.1016\/j.molimm.2012.03.015_bib0075","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1016\/j.bbapap.2008.07.005","article-title":"Thermal unfolding studies of a phytocyanin","volume":"1784","author":"Guzzi","year":"2008","journal-title":"Biochimica et Biophysica Acta (BBA)-Proteins & Proteomics"},{"key":"10.1016\/j.molimm.2012.03.015_bib0080","doi-asserted-by":"crossref","first-page":"3834","DOI":"10.1016\/S0264-410X(01)00135-9","article-title":"Experimental utility of rabies virus-neutralizing human monoclonal antibodies in post-exposure prophylaxis","volume":"19","author":"Hanlon","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.molimm.2012.03.015_bib0085","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1080\/01621459.1958.10501452","article-title":"Nonparametric estimation from incomplete observations","volume":"53","author":"Kaplan","year":"1958","journal-title":"Journal of the American Statistical Association"},{"key":"10.1016\/j.molimm.2012.03.015_bib0090","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1007\/BF01863914","article-title":"Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche","volume":"162","author":"Karber","year":"1931","journal-title":"Naunyn Schmiedebergs Arch. Pharmacol."},{"key":"10.1016\/j.molimm.2012.03.015_bib0095","first-page":"6176","article-title":"Improved tumor targeting with chemically cross-linked recombinant antibody fragments","volume":"54","author":"King","year":"1994","journal-title":"Cancer Res."},{"key":"10.1016\/j.molimm.2012.03.015_bib0100","first-page":"360","article-title":"Re-evaluating the burden of rabies in Africa and Asia","volume":"83","author":"Knobel","year":"2005","journal-title":"Bull. World Health Organ."},{"key":"10.1016\/j.molimm.2012.03.015_bib0105","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1182\/blood.V83.2.426.426","article-title":"Recombinant immunotoxins containing anti-Tac (Fv) and derivatives of Pseudomonas exotoxin produce complete regression in mice of an interleukin-2 receptor-expressing human carcinoma","volume":"83","author":"Kreitman","year":"1994","journal-title":"Blood"},{"key":"10.1016\/j.molimm.2012.03.015_bib0110","series-title":"Instant Notes in Immunology","author":"Lydyard","year":"1999"},{"key":"10.1016\/j.molimm.2012.03.015_bib0115","series-title":"Laboratory Techniques in Rabies","author":"Meslin","year":"1996"},{"key":"10.1016\/j.molimm.2012.03.015_bib0120","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1177\/019262339902700507","article-title":"Comparisons of the intraocular tissue distribution, pharmacokinetics, and safety of 125I-labeled full-length and Fab antibodies in rhesus monkeys following intravitreal administration","volume":"27","author":"Mordenti","year":"1999","journal-title":"Toxicol. Pathol."},{"key":"10.1016\/j.molimm.2012.03.015_bib0125","doi-asserted-by":"crossref","first-page":"3026","DOI":"10.1016\/j.vaccine.2004.11.071","article-title":"Production of rabies neutralizing antibody in hen's eggs using a part of the G protein expressed in Escherichia coli","volume":"23","author":"Motoi","year":"2005","journal-title":"Vaccine"},{"key":"10.1016\/j.molimm.2012.03.015_bib0130","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.ijid.2006.09.013","article-title":"Development and evaluation of a competitive ELISA for estimation of rabies neutralizing antibodies after post-exposure rabies vaccination in humans","volume":"11","author":"Muhamuda","year":"2007","journal-title":"Int. J. Infect. Dis."},{"key":"10.1016\/j.molimm.2012.03.015_bib0135","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0166-0934(97)00099-2","article-title":"Phage-displayed and soluble mouse scFv fragments neutralize rabies virus","volume":"67","author":"Muller","year":"1997","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.molimm.2012.03.015_bib0140","first-page":"1536","article-title":"Pharmacokinetics and biodistribution of engineered single-chain antibody constructs of MAb CC49 in colon carcinoma xenografts","volume":"40","author":"Pavlinkova","year":"1999","journal-title":"J. Nucl. Med."},{"key":"10.1016\/j.molimm.2012.03.015_bib0145","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/S0264-410X(85)90224-5","article-title":"Rabies immunosome (subunit vaccine) structure and immunogenicity. Pre- and post-exposure protection studies","volume":"3","author":"Perrin","year":"1985","journal-title":"Vaccine"},{"key":"10.1016\/j.molimm.2012.03.015_bib0150","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1086\/375247","article-title":"Development of a cocktail of recombinant-expressed human rabies virus-neutralizing monoclonal antibodies for postexposure prophylaxis of rabies","volume":"188","author":"Prosniak","year":"2003","journal-title":"Journal of Infectious Diseases"},{"key":"10.1016\/j.molimm.2012.03.015_bib0155","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1096\/fasebj.9.1.7821762","article-title":"Single-chain Fvs","volume":"9","author":"Raag","year":"1995","journal-title":"FASEB Journal"},{"key":"10.1016\/j.molimm.2012.03.015_bib0160","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1046\/j.1365-2249.2001.01515.x","article-title":"Selection of single chain variable fragments (scFv) against the glycoprotein antigen of the rabies virus from a human synthetic scFv phage display library and their fusion with the Fc region of human IgG1","volume":"125","author":"Ray","year":"2001","journal-title":"Clinical & Experimental Immunology"},{"key":"10.1016\/j.molimm.2012.03.015_bib0165","doi-asserted-by":"crossref","first-page":"18327","DOI":"10.1016\/S0021-9258(17)32310-4","article-title":"Improved binding and antitumor activity of a recombinant anti-erbB2 immunotoxin by disulfide stabilization of the Fv fragment","volume":"269","author":"Reiter","year":"1994","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/j.molimm.2012.03.015_bib0170","doi-asserted-by":"crossref","first-page":"5451","DOI":"10.1021\/bi00184a014","article-title":"Stabilization of the Fv fragments in recombinant immunotoxins by disulfide bonds engineered into conserved framework regions","volume":"33","author":"Reiter","year":"1994","journal-title":"Biochemistry"},{"key":"10.1016\/j.molimm.2012.03.015_bib0175","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1093\/protein\/7.5.697","article-title":"Engineering interchain disulfide bonds into conserved framework regions of Fv fragments: improved biochemical characteristics of recombinant immunotoxins containing disulfide-stabilized Fv","volume":"7","author":"Reiter","year":"1994","journal-title":"Protein Eng."},{"key":"10.1016\/j.molimm.2012.03.015_bib0180","first-page":"245","article-title":"Antibody engineering of recombinant Fv immunotoxins for improved targeting of cancer: disulfide-stabilized Fv immunotoxins","volume":"2","author":"Reiter","year":"1996","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.molimm.2012.03.015_bib0185","doi-asserted-by":"crossref","first-page":"2626","DOI":"10.1056\/NEJMcp042140","article-title":"Clinical practice. Prophylaxis against rabies","volume":"351","author":"Rupprecht","year":"2004","journal-title":"New England Journal of Medicine"},{"key":"10.1016\/j.molimm.2012.03.015_bib0190","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1172\/JCI114260","article-title":"Use of mouse anti-rabies monoclonal antibodies in postexposure treatment of rabies","volume":"84","author":"Schumacher","year":"1989","journal-title":"Journal of Clinical Investigation"},{"key":"10.1016\/j.molimm.2012.03.015_bib0195","first-page":"1","article-title":"Effective protection of monkeys against death from street virus by post-exposure administration of tissue-culture rabies vaccine","volume":"45","author":"Sikes","year":"1971","journal-title":"Bull. World Health Organ."},{"key":"10.1016\/j.molimm.2012.03.015_bib0200","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1126\/science.3285470","article-title":"Assembly of a functional immunoglobulin Fv fragment in Escherichia coli","volume":"240","author":"Skerra","year":"1988","journal-title":"Science"},{"key":"10.1016\/j.molimm.2012.03.015_bib0205","first-page":"535","article-title":"A rapid reproducible test for determining rabies neutralizing antibody","volume":"48","author":"Smith","year":"1973","journal-title":"Bull. World Health Organ."},{"key":"10.1016\/j.molimm.2012.03.015_bib0210","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/j.2044-8295.1908.tb00176.x","article-title":"The method of \u2018right and wrong cases\u2019 (\u2018constant stimuli\u2019) without Gauss's formulae","volume":"2","author":"Spearman","year":"1908","journal-title":"British Journal of Psychology, 1904\u20131920"},{"key":"10.1016\/j.molimm.2012.03.015_bib0215","series-title":"Laboratory Techniques in Rabies","article-title":"Characteristics and molecular biology of the rabies virus","author":"Tordo","year":"1996"},{"key":"10.1016\/j.molimm.2012.03.015_bib0220","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0168-1656(00)00244-3","article-title":"Protein folding and stability investigated by fluorescence, circular dichroism (CD), and nuclear magnetic resonance (NMR) spectroscopy: the flavodoxin story","volume":"79","author":"van Mierlo","year":"2000","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.molimm.2012.03.015_bib0225","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/S0140-6736(04)15792-9","article-title":"Rabies and other lyssavirus diseases","volume":"363","author":"Warrell","year":"2004","journal-title":"Lancet"},{"key":"10.1016\/j.molimm.2012.03.015_bib0230","unstructured":"WHO, 1957. World Health Organization Expert Committee on Rabies. World Health Organization technical report, ser. 121, World Health Organization, Geneva."},{"key":"10.1016\/j.molimm.2012.03.015_bib0235","doi-asserted-by":"crossref","first-page":"269","DOI":"10.4049\/jimmunol.110.1.269","article-title":"Antigenic properties of rabies virus components","volume":"110","author":"Wiktor","year":"1973","journal-title":"Journal of Immunology"},{"key":"10.1016\/j.molimm.2012.03.015_bib0240","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1580\/1080-6032(2000)011[0031:RU]2.3.CO;2","article-title":"Rabies update","volume":"11","author":"Wilkerson","year":"2000","journal-title":"Wilderness & Environmental Medicine"},{"key":"10.1016\/j.molimm.2012.03.015_bib0245","first-page":"156","article-title":"Affinity maturation of a human single-chain Fv by phage display","volume":"21","author":"Xiaoping","year":"2001","journal-title":"Chinese Journal of Microbiology and Immunology"}],"container-title":["Molecular Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0161589012001915?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0161589012001915?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T21:04:18Z","timestamp":1687381458000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0161589012001915"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["S0161589012001915"],"URL":"https:\/\/doi.org\/10.1016\/j.molimm.2012.03.015","relation":{},"ISSN":["0161-5890"],"issn-type":[{"value":"0161-5890","type":"print"}],"subject":[],"published":{"date-parts":[[2012,6]]}}}