{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:07:33Z","timestamp":1782173253827,"version":"3.54.5"},"reference-count":25,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100000185","name":"Defense Advanced Research Projects Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000185","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Immunology"],"published-print":{"date-parts":[[2013,1]]},"DOI":"10.1016\/j.molimm.2012.07.010","type":"journal-article","created":{"date-parts":[[2012,8,13]],"date-time":"2012-08-13T16:42:28Z","timestamp":1344876148000},"page":"118-125","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"title":["Selection and evaluation of single domain antibodies toward MS2 phage and coat protein"],"prefix":"10.1016","volume":"53","author":[{"given":"Jinny L.","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Zabetakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen R.","family":"Goldman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George P.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.molimm.2012.07.010_bib0005","doi-asserted-by":"crossref","first-page":"7202","DOI":"10.1021\/ac100961x","article-title":"Binding kinetics of antiricin single domain antibodies and improved detection using a B chain specific binder","volume":"82","author":"Anderson","year":"2010","journal-title":"Analytical Chemistry"},{"key":"10.1016\/j.molimm.2012.07.010_bib0010","doi-asserted-by":"crossref","first-page":"9604","DOI":"10.1021\/ac8019398","article-title":"Development of antiricin single domain antibodies toward detection and therapeutic reagents","volume":"80","author":"Anderson","year":"2008","journal-title":"Analytical Chemistry"},{"key":"10.1016\/j.molimm.2012.07.010_bib0015","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S0014-5793(97)01062-4","article-title":"Selection and identification of single domain antibody fragments from camel heavy-chain antibodies","volume":"414","author":"Arbabi Ghahroudi","year":"1997","journal-title":"FEBS Letters"},{"key":"10.1016\/j.molimm.2012.07.010_bib0020","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1038\/89281","article-title":"Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS)","volume":"19","author":"Chen","year":"2001","journal-title":"Nature Biotechnology"},{"key":"10.1016\/j.molimm.2012.07.010_bib0025","first-page":"99","article-title":"Bacteriophage Ms2 RNA: nucleotide sequence of the end of the a protein gene and the intercistronic region","volume":"241","author":"Contreras","year":"1973","journal-title":"Nature: New Biology"},{"key":"10.1016\/j.molimm.2012.07.010_bib0030","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0008818","article-title":"Llama single domain antibodies specific for the 7 botulinum neurotoxin serotypes as heptaplex immunoreagents","volume":"5","author":"Conway","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.molimm.2012.07.010_bib0035","doi-asserted-by":"crossref","first-page":"10881","DOI":"10.1093\/nar\/16.22.10881","article-title":"Multiple sequence alignment with hierarchical-clustering","volume":"16","author":"Corpet","year":"1988","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/j.molimm.2012.07.010_bib0040","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1110\/ps.34602","article-title":"Single-domain antibody fragments with high conformational stability","volume":"11","author":"Dumoulin","year":"2002","journal-title":"Protein Science"},{"issue":"24","key":"10.1016\/j.molimm.2012.07.010_bib0125","doi-asserted-by":"crossref","first-page":"8245","DOI":"10.1021\/ac0610053","article-title":"Facile generation of heat stable antiviral and antitoxin single domain antibodies from a semi-synthetic llama library","volume":"78","author":"Goldman","year":"2006","journal-title":"Analytical Chemistry"},{"key":"10.1016\/j.molimm.2012.07.010_bib0045","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/1472-6750-11-86","article-title":"Isolation of a highly thermal stable lama single domain antibody specific for Staphylococcus aureus enterotoxin B","volume":"11","author":"Graef","year":"2011","journal-title":"BMC Biotechnology"},{"key":"10.1016\/j.molimm.2012.07.010_bib0050","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1002\/j.1460-2075.1993.tb05706.x","article-title":"Human anti-self antibodies with high specificity from phage display libraries","volume":"12","author":"Griffiths","year":"1993","journal-title":"EMBO Journal"},{"key":"10.1016\/j.molimm.2012.07.010_bib0055","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1038\/363446a0","article-title":"Naturally occurring antibodies devoid of light chains","volume":"363","author":"Hamers-Casterman","year":"1993","journal-title":"Nature"},{"key":"10.1016\/j.molimm.2012.07.010_bib0060","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0022-1759(03)00100-5","article-title":"Isolation and expression of recombinant antibody fragments to the biological warfare pathogen Brucella melitensis","volume":"276","author":"Hayhurst","year":"2003","journal-title":"Journal of Immunological Methods"},{"key":"10.1016\/j.molimm.2012.07.010_bib0065","doi-asserted-by":"crossref","first-page":"3256","DOI":"10.4049\/jimmunol.174.6.3256","article-title":"Protein conformation significantly influences immune responses to prion protein","volume":"174","author":"Khalili-Shirazi","year":"2005","journal-title":"Journal of Immunology"},{"key":"10.1016\/j.molimm.2012.07.010_bib0070","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1016\/j.molimm.2006.07.299","article-title":"Selection of cholera toxin specific IgNAR single-domain antibodies from a naive shark library","volume":"44","author":"Liu","year":"2007","journal-title":"Molecular Immunology"},{"key":"10.1016\/j.molimm.2012.07.010_bib0075","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1038\/237082a0","article-title":"Nucleotide sequence of the gene coding for the bacteriophage MS2 coat protein","volume":"237","author":"Min Jou","year":"1972","journal-title":"Nature"},{"key":"10.1016\/j.molimm.2012.07.010_bib0080","first-page":"277","article-title":"Single domain camel antibodies: current status","volume":"74","author":"Muyldermans","year":"2001","journal-title":"Journal of Biotechnology"},{"key":"10.1016\/j.molimm.2012.07.010_bib0085","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0969-2126(01)00156-3","article-title":"Crystal structure of the MS2 coat protein dimer: implications for RNA binding and virus assembly","volume":"3","author":"Ni","year":"1995","journal-title":"Structure"},{"key":"10.1016\/j.molimm.2012.07.010_bib0090","series-title":"DTIC","article-title":"Next generation recombinant antibodies and antigens for the detection of biological threat agents and simulants","author":"O\u2019Connell","year":"2002"},{"key":"10.1016\/j.molimm.2012.07.010_bib0095","doi-asserted-by":"crossref","first-page":"5684","DOI":"10.1016\/S0021-9258(19)39417-7","article-title":"Translational repression by bacteriophage MS2 coat protein expressed from a plasmid. A system for genetic analysis of a protein\u2013RNA interaction","volume":"265","author":"Peabody","year":"1990","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/j.molimm.2012.07.010_bib0100","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1093\/nar\/20.7.1649","article-title":"Control of translational repression by protein\u2013protein interactions","volume":"20","author":"Peabody","year":"1992","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/j.molimm.2012.07.010_bib0105","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.jmb.2008.04.049","article-title":"Immunogenic display of diverse peptides on virus-like particles of RNA phage MS2","volume":"380","author":"Peabody","year":"2008","journal-title":"Journal of Molecular Biology"},{"key":"10.1016\/j.molimm.2012.07.010_bib0110","doi-asserted-by":"crossref","first-page":"S213","DOI":"10.1086\/520586","article-title":"Rapid assembly of sensitive antigen-capture assays for Marburg virus, using in vitro selection of llama single-domain antibodies, at biosafety level 4","volume":"196","author":"Sherwood","year":"2007","journal-title":"Journal of Infectious Diseases"},{"key":"10.1016\/j.molimm.2012.07.010_bib0115","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/345036a0","article-title":"The three-dimensional structure of the bacterial virus MS2","volume":"345","author":"Valegard","year":"1990","journal-title":"Nature"},{"key":"10.1016\/j.molimm.2012.07.010_bib0120","doi-asserted-by":"crossref","first-page":"e32801","DOI":"10.1371\/journal.pone.0032801","article-title":"Rugged single domain antibody detection elements for Bacillus anthracis spores and vegetative cells","volume":"7","author":"Walper","year":"2012","journal-title":"PLoS One"}],"container-title":["Molecular Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0161589012003574?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0161589012003574?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T13:27:32Z","timestamp":1758979652000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0161589012003574"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1]]},"references-count":25,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["S0161589012003574"],"URL":"https:\/\/doi.org\/10.1016\/j.molimm.2012.07.010","relation":{},"ISSN":["0161-5890"],"issn-type":[{"value":"0161-5890","type":"print"}],"subject":[],"published":{"date-parts":[[2013,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Selection and evaluation of single domain antibodies toward MS2 phage and coat protein","name":"articletitle","label":"Article Title"},{"value":"Molecular Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.molimm.2012.07.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}