{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:09:04Z","timestamp":1789186144930,"version":"build-2803163510"},"reference-count":34,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100017515","name":"Heidelberger Stiftung Chirurgie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017515","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010345","name":"B. Braun-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010345","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Immunology"],"published-print":{"date-parts":[[2013,12]]},"DOI":"10.1016\/j.molimm.2013.06.021","type":"journal-article","created":{"date-parts":[[2013,8,2]],"date-time":"2013-08-02T06:09:10Z","timestamp":1375423750000},"page":"649-659","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"title":["Gender-specific immunological effects of the phosphodiesterase 5 inhibitor sildenafil in healthy mice"],"prefix":"10.1016","volume":"56","author":[{"given":"Svetlana","family":"Karakhanova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhui","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Link","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Soltek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"von Ahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktor","family":"Umansky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Werner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandr V.","family":"Bazhin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.molimm.2013.06.021_bib0005","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1158\/1541-7786.MCR-07-0140","article-title":"Visible light modulates the expression of cancer-retina antigens","volume":"6","author":"Bazhin","year":"2008","journal-title":"Molecular Cancer Research"},{"issue":"Suppl. 1","key":"10.1016\/j.molimm.2013.06.021_bib0010","first-page":"S252","article-title":"Phosphodiesterase inhibitors","volume":"147","author":"Boswell-Smith","year":"2006","journal-title":"British Journal of Pharmacology"},{"issue":"2 (Suppl. 2)","key":"10.1016\/j.molimm.2013.06.021_bib0015","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/S0090-4295(02)01686-2","article-title":"Phosphodiesterase type 5 as a pharmacologic target in erectile dysfunction","volume":"60","author":"Corbin","year":"2002","journal-title":"Urology"},{"key":"10.1016\/j.molimm.2013.06.021_bib0020","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1111\/j.1749-6632.1999.tb07667.x","article-title":"Sex hormones modulate inflammatory mediators produced by macrophages","volume":"876","author":"D\u2019Agostino","year":"1999","journal-title":"Annals of the New York Academy of Sciences"},{"issue":"12","key":"10.1016\/j.molimm.2013.06.021_bib0025","doi-asserted-by":"crossref","first-page":"4574","DOI":"10.1182\/blood-2008-04-148692","article-title":"Estrogen inhibits dendritic cell maturation to RNA viruses","volume":"112","author":"Escribese","year":"2008","journal-title":"Blood"},{"issue":"9","key":"10.1016\/j.molimm.2013.06.021_bib0030","first-page":"737","article-title":"The X-files in immunity: sex-based differences predispose immune responses","volume":"8","author":"Fish","year":"2008","journal-title":"Nature Reviews"},{"issue":"3","key":"10.1016\/j.molimm.2013.06.021_bib0035","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1124\/pr.110.002907","article-title":"cGMP-dependent protein kinases and cGMP phosphodiesterases in nitric oxide and cGMP action","volume":"62","author":"Francis","year":"2010","journal-title":"Pharmacological Reviews"},{"issue":"3","key":"10.1016\/j.molimm.2013.06.021_bib0040","first-page":"162","article-title":"Myeloid-derived suppressor cells as regulators of the immune system","volume":"9","author":"Gabrilovich","year":"2009","journal-title":"Nature Reviews"},{"issue":"1","key":"10.1016\/j.molimm.2013.06.021_bib0045","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/0192-0561(88)90145-2","article-title":"Effect of sex hormones on NK and ADCC activity of mice","volume":"10","author":"Hou","year":"1988","journal-title":"International Journal of Immunopharmacology"},{"issue":"2","key":"10.1016\/j.molimm.2013.06.021_bib0050","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0006-2952(92)90010-G","article-title":"Effect of age and gender on the activity of human hepatic CYP3A","volume":"44","author":"Hunt","year":"1992","journal-title":"Biochemical Pharmacology"},{"issue":"3","key":"10.1016\/j.molimm.2013.06.021_bib0055","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1046\/j.1365-2125.2001.00318.x","article-title":"Identification of the cytochrome P450 enzymes involved in the N-demethylation of sildenafil","volume":"51","author":"Hyland","year":"2001","journal-title":"British Journal of Clinical Pharmacology"},{"issue":"4","key":"10.1016\/j.molimm.2013.06.021_bib0060","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1210\/en.2005-0648","article-title":"Sex-dependent effect of melatonin on systemic erythematosus lupus developed in Mrl\/Mpj-Faslpr mice: it ameliorates the disease course in females, whereas it exacerbates it in males","volume":"147","author":"Jimenez-Caliani","year":"2006","journal-title":"Endocrinology"},{"issue":"6","key":"10.1016\/j.molimm.2013.06.021_bib0065","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1210\/en.2011-2120","article-title":"Immune cells have sex and so should journal articles","volume":"153","author":"Klein","year":"2012","journal-title":"Endocrinology"},{"issue":"8","key":"10.1016\/j.molimm.2013.06.021_bib0070","first-page":"594","article-title":"The X chromosome in immune functions: when a chromosome makes the difference","volume":"10","author":"Libert","year":"2010","journal-title":"Nature Reviews"},{"issue":"5","key":"10.1016\/j.molimm.2013.06.021_bib0075","doi-asserted-by":"crossref","first-page":"2747","DOI":"10.4049\/jimmunol.1000496","article-title":"B7-H1-dependent sex-related differences in tumor immunity and immunotherapy responses","volume":"185","author":"Lin","year":"2010","journal-title":"Journal of Immunology"},{"issue":"41","key":"10.1016\/j.molimm.2013.06.021_bib0080","doi-asserted-by":"crossref","first-page":"17111","DOI":"10.1073\/pnas.1108121108","article-title":"Chronic inflammation promotes myeloid-derived suppressor cell activation blocking antitumor immunity in transgenic mouse melanoma model","volume":"108","author":"Meyer","year":"2011","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"3","key":"10.1016\/j.molimm.2013.06.021_bib0085","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1161\/01.RES.0000256354.95791.f1","article-title":"Overview of PDEs and their regulation","volume":"100","author":"Omori","year":"2007","journal-title":"Circulation Research"},{"issue":"3","key":"10.1016\/j.molimm.2013.06.021_bib0090","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.4049\/jimmunol.172.3.1426","article-title":"Estrogen preferentially promotes the differentiation of CD11c+ CD11b(intermediate) dendritic cells from bone marrow precursors","volume":"172","author":"Paharkova-Vatchkova","year":"2004","journal-title":"Journal of Immunology"},{"issue":"26","key":"10.1016\/j.molimm.2013.06.021_bib0095","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.2174\/092986706778742927","article-title":"Cardioprotection with sildenafil: implications for clinical practice","volume":"13","author":"Raja","year":"2006","journal-title":"Current Medicinal Chemistry"},{"issue":"3","key":"10.1016\/j.molimm.2013.06.021_bib0100","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1095\/biolreprod.107.063545","article-title":"Testosterone reduces macrophage expression in the mouse of toll-like receptor 4, a trigger for inflammation and innate immunity","volume":"78","author":"Rettew","year":"2008","journal-title":"Biology of Reproduction"},{"issue":"27","key":"10.1016\/j.molimm.2013.06.021_bib0105","doi-asserted-by":"crossref","first-page":"4640","DOI":"10.2174\/092986712803306475","article-title":"Therapeutic strategies in pulmonary hypertension of the newborn: where are we now?","volume":"19","author":"Sanchez Luna","year":"2012","journal-title":"Current Medicinal Chemistry"},{"issue":"1","key":"10.1016\/j.molimm.2013.06.021_bib0110","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1182\/blood-2002-01-0075","article-title":"Sildenafil and vardenafil, types 5 and 6 phosphodiesterase inhibitors, induce caspase-dependent apoptosis of B-chronic lymphocytic leukemia cells","volume":"101","author":"Sarfati","year":"2003","journal-title":"Blood"},{"issue":"4","key":"10.1016\/j.molimm.2013.06.021_bib0115","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1517\/17425255.4.4.413","article-title":"Sex-specific differences in CYP450 isoforms in humans","volume":"4","author":"Scandlyn","year":"2008","journal-title":"Expert Opinion on Drug Metabolism & Toxicology"},{"issue":"2","key":"10.1016\/j.molimm.2013.06.021_bib0120","doi-asserted-by":"crossref","first-page":"222","DOI":"10.2174\/156802610790411009","article-title":"Phosphodiesterase inhibitors as potential cognition enhancing agents","volume":"10","author":"Schmidt","year":"2010","journal-title":"Current Topics in Medicinal Chemistry"},{"issue":"6","key":"10.1016\/j.molimm.2013.06.021_bib0125","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nature Protocols"},{"issue":"12","key":"10.1016\/j.molimm.2013.06.021_bib0130","doi-asserted-by":"crossref","first-page":"2691","DOI":"10.1084\/jem.20061104","article-title":"Phosphodiesterase-5 inhibition augments endogenous antitumor immunity by reducing myeloid-derived suppressor cell function","volume":"203","author":"Serafini","year":"2006","journal-title":"Journal of Experimental Medicine"},{"key":"10.1016\/j.molimm.2013.06.021_bib0135","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/ijc.27990","article-title":"Low-dose gemcitabine depletes regulatory T cells and improves survival in the orthotopic Panc02 model of pancreatic cancer","volume":"133","author":"Shevchenko","year":"2012","journal-title":"International Journal of Cancer"},{"issue":"2","key":"10.1016\/j.molimm.2013.06.021_bib0140","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1016\/S0021-9258(19)77423-7","article-title":"Fractionation and characterization of a cyclic adenine ribonucleotide formed by tissue particles","volume":"232","author":"Sutherland","year":"1958","journal-title":"Journal of Biological Chemistry"},{"issue":"3","key":"10.1016\/j.molimm.2013.06.021_bib0145","doi-asserted-by":"crossref","first-page":"507","DOI":"10.3109\/08923970903359850","article-title":"The effects of selective and nonselective phosphodiesterase inhibitors on phagocytic cells in mice","volume":"32","author":"Szczypka","year":"2010","journal-title":"Immunopharmacology and Immunotoxicology"},{"issue":"6","key":"10.1016\/j.molimm.2013.06.021_bib0150","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1016\/S1734-1140(10)70377-7","article-title":"Modulating effects of nonselective and selective phosphodiesterase inhibitors on lymphocyte subsets and humoral immune response in mice","volume":"62","author":"Szczypka","year":"2010","journal-title":"Pharmacological Reports"},{"issue":"3","key":"10.1016\/j.molimm.2013.06.021_bib0155","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1038\/hr.2011.189","article-title":"Sex-related differences in pharmacokinetics and pharmacodynamics of anti-hypertensive drugs","volume":"35","author":"Ueno","year":"2012","journal-title":"Hypertension Research"},{"issue":"2","key":"10.1016\/j.molimm.2013.06.021_bib0160","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1124\/mol.109.056705","article-title":"Sex differences in the expression of hepatic drug metabolizing enzymes","volume":"76","author":"Waxman","year":"2009","journal-title":"Molecular Pharmacology"},{"issue":"1","key":"10.1016\/j.molimm.2013.06.021_bib0165","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.molimm.2012.04.010","article-title":"In vivo immunoregulatory properties of the novel mitochondria-targeted antioxidant SkQ1","volume":"52","author":"Yang","year":"2012","journal-title":"Molecular Immunology"},{"issue":"4","key":"10.1016\/j.molimm.2013.06.021_bib0170","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1161\/01.STR.0000158923.19956.73","article-title":"Functional recovery in aged and young rats after embolic stroke: treatment with a phosphodiesterase type 5 inhibitor","volume":"36","author":"Zhang","year":"2005","journal-title":"Stroke"}],"container-title":["Molecular Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0161589013004471?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0161589013004471?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T13:27:49Z","timestamp":1758979669000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0161589013004471"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":34,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["S0161589013004471"],"URL":"https:\/\/doi.org\/10.1016\/j.molimm.2013.06.021","relation":{},"ISSN":["0161-5890"],"issn-type":[{"value":"0161-5890","type":"print"}],"subject":[],"published":{"date-parts":[[2013,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Gender-specific immunological effects of the phosphodiesterase 5 inhibitor sildenafil in healthy mice","name":"articletitle","label":"Article Title"},{"value":"Molecular Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.molimm.2013.06.021","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}