{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:45:35Z","timestamp":1792183535847,"version":"4.4.0"},"reference-count":41,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31060335"],"award-info":[{"award-number":["31060335"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Technologies Research and Development Program of China","award":["2012BAD12B07-4"],"award-info":[{"award-number":["2012BAD12B07-4"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Immunology"],"published-print":{"date-parts":[[2015,12]]},"DOI":"10.1016\/j.molimm.2015.10.016","type":"journal-article","created":{"date-parts":[[2015,11,14]],"date-time":"2015-11-14T22:30:53Z","timestamp":1447540253000},"page":"684-691","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"PC","title":["Immunogenicity and protective efficacy of a recombinant adenoviral based vaccine expressing heat-stable enterotoxin (STa) and K99 adhesion antigen of enterotoxigenic Escherichia coli in mice"],"prefix":"10.1016","volume":"68","author":[{"given":"Guangcun","family":"Deng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaowen","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meihui","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujiong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.molimm.2015.10.016_bib0005","article-title":"Vaccines for preventing enterotoxigenic E. coli (ETEC) diarrhoea","volume":"7","author":"Ahmed","year":"2013","journal-title":"Cochrane Database Syst. Rev."},{"key":"10.1016\/j.molimm.2015.10.016_bib0010","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1517\/14712598.2015.993374","article-title":"Adenoviruses as gene\/vaccine delivery vectors: promises and pitfalls","author":"Appaiahgari","year":"2015","journal-title":"Expert Opin. Biol. Ther."},{"key":"10.1016\/j.molimm.2015.10.016_bib0015","first-page":"15","article-title":"Vaccines for enterotoxigenic E. coli: current status","author":"Boedeker","year":"2005","journal-title":"Curr. Opin. Gastroenterol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0020","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1111\/j.1348-0421.2005.tb03687.x","article-title":"Lactobacillus acidophilus expressing recombinant K99 adhesive fimbriae has an inhibitory effect on adhesion of enterotoxigenic E. coli","author":"Chu","year":"2005","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0025","doi-asserted-by":"crossref","first-page":"71","DOI":"10.4161\/gmic.19182","article-title":"Infection strategies of enteric pathogenic E. coli","author":"Clements","year":"2012","journal-title":"Gut Microbes"},{"key":"10.1016\/j.molimm.2015.10.016_bib0030","doi-asserted-by":"crossref","first-page":"4492","DOI":"10.1128\/IAI.68.8.4492-4497.2000","article-title":"Safety and immunogenicity of two different lots of the oral, killed enterotoxigenic E. coli-cholera toxin B subunit vaccine in Israeli young adults","author":"Cohen","year":"2000","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0035","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1128\/IAI.33.3.877-883.1981","article-title":"Purification, characterization, and partial covalent structure of E. coli adhesive antigen K99","author":"de Graaf","year":"1981","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0040","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1128\/IAI.36.2.751-758.1982","article-title":"Production, purification, and characterization of the fimbrial adhesive antigen F41 isolated from calf enteropathogenic E. coli strain B41M","author":"de Graaf","year":"1982","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0045","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1079\/AHR2005105","article-title":"E. coli in postweaning diarrhea in pigs: an update on bacterial types, pathogenesis, and prevention strategies","author":"Fairbrother","year":"2005","journal-title":"Anim. Health Res. Rev."},{"key":"10.1016\/j.molimm.2015.10.016_bib0050","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1016\/S0264-410X(00)00264-4","article-title":"Microencapsulated enterotoxigenic E. coli and detached fimbriae for peroral vaccination of pigs","author":"Felder","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.molimm.2015.10.016_bib0055","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1586\/14760584.2014.905745","article-title":"Novel antigens for enterotoxigenic E. coli vaccines","author":"Fleckenstein","year":"2014","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.molimm.2015.10.016_bib0060","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1128\/MMBR.46.2.129-161.1982","article-title":"Host-specific fimbrial adhesins of noninvasive enterotoxigenic E. coli strains","author":"Gaastra","year":"1982","journal-title":"Microbiol. Rev."},{"key":"10.1016\/j.molimm.2015.10.016_bib0065","doi-asserted-by":"crossref","first-page":"2732","DOI":"10.1016\/j.vaccine.2005.10.014","article-title":"A review of vaccine research and development: human enteric infections","author":"Girard","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.molimm.2015.10.016_bib0070","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1007\/s10529-009-9966-8","article-title":"Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice","author":"Hu","year":"2009","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.molimm.2015.10.016_bib0075","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1128\/JB.146.2.784-789.1981","article-title":"E. coli 987P pilus: purification and partial characterization","author":"Isaacson","year":"1981","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0080","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s11248-006-0010-7","article-title":"Glycosylated F4 (K88) fimbrial adhesin FaeG expressed in barley endosperm induces ETEC-neutralizing antibodies in mice","author":"Joensuu","year":"2006","journal-title":"Transgenic Res."},{"key":"10.1016\/j.molimm.2015.10.016_bib0085","doi-asserted-by":"crossref","first-page":"2609","DOI":"10.2174\/15680266113136660186","article-title":"Mucosal vaccines: where do we stand","author":"Kraehenbuhl","year":"2013","journal-title":"Curr. Top Med. Chem."},{"key":"10.1016\/j.molimm.2015.10.016_bib0090","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.1128\/jb.172.5.2427-2432.1990","article-title":"Secretion of methanol-insoluble heat-stable enterotoxin (STB): energy-and secA-dependent conversion of pre-STB to an intermediate indistinguishable from the extracellular toxin","author":"Kupersztoch","year":"1990","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0095","doi-asserted-by":"crossref","first-page":"e2705","DOI":"10.1371\/journal.pntd.0002705","article-title":"Estimating diarrheal illness and deaths attributable to Shigellae and enterotoxigenic E. coli among older children, adolescents, and adults in South Asia and Africa","author":"Lamberti","year":"2014","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.molimm.2015.10.016_bib0100","doi-asserted-by":"crossref","first-page":"e72788","DOI":"10.1371\/journal.pone.0072788","article-title":"Global causes of diarrheal disease mortality in children <5 years of age: a systematic review","author":"Lanata","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.molimm.2015.10.016_bib0105","doi-asserted-by":"crossref","first-page":"4491","DOI":"10.1128\/AEM.02672-08","article-title":"Induction of immune responses in mice after oral immunization with recombinant Lactobacillus casei strains expressing enterotoxigenic E. coli F41 fimbrial protein","author":"Liu","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0110","doi-asserted-by":"crossref","first-page":"4002","DOI":"10.1128\/IAI.00165-11","article-title":"Heat-labile- and heat-stable-toxoid fusions (LTR(1)(9)(2)G-STaP(1)(3)F) of human enterotoxigenic E. coli elicit neutralizing antitoxin antibodies","author":"Liu","year":"2011","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0115","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1128\/CMR.11.1.142","article-title":"Diarrheagenic E. coli","author":"Nataro","year":"1998","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.molimm.2015.10.016_bib0120","doi-asserted-by":"crossref","first-page":"e96896","DOI":"10.1371\/journal.pone.0096896","article-title":"Molecular characterization of enterotoxigenic E. coli strains isolated from diarrheal patients in Korea during 2003\u20132011","author":"Oh","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.molimm.2015.10.016_bib0125","doi-asserted-by":"crossref","first-page":"2394","DOI":"10.1016\/S0264-410X(03)00077-X","article-title":"Safety and immunogenicity of an oral, inactivated enterotoxigenic E. coli plus cholera toxin B subunit vaccine in Bangladeshi children 18\u201336 months of age","author":"Qadri","year":"2003","journal-title":"Vaccine"},{"key":"10.1016\/j.molimm.2015.10.016_bib0130","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1128\/CMR.18.3.465-483.2005","article-title":"Enterotoxigenic E. coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention","author":"Qadri","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.molimm.2015.10.016_bib0135","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1128\/IAI.01394-13","article-title":"Characterization of heat-stable (STa) toxoids of enterotoxigenic E. coli fused to double mutant heat-labile toxin peptide in inducing neutralizing Anti-STa antibodies","author":"Ruan","year":"2014","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0140","first-page":"337","article-title":"Avirulent K88 (F4)+ E. coli strains constructed to express modified enterotoxins protect young piglets from challenge with a virulent enterotoxigenic E. coli strain that expresses the same adhesion and enterotoxins","author":"Santiago-Mateo","year":"2012","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0145","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1086\/314543","article-title":"Oral, inactivated, whole cell enterotoxigenic E. coli plus cholera toxin B subunit vaccine: results of the initial evaluation in children. PRIDE study Group","author":"Savarino","year":"1999","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.molimm.2015.10.016_bib0150","doi-asserted-by":"crossref","first-page":"609","DOI":"10.4269\/ajtmh.2009.80.609","article-title":"Global etiology of travelers\u2019 diarrhea: systematic review from 1973 to the present","author":"Shah","year":"2009","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.molimm.2015.10.016_bib0155","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.vetimm.2003.08.003","article-title":"Enteric-coated pellets of F4 fimbriae for oral vaccination of suckling piglets against enterotoxigenic E. coli infections","author":"Snoeck","year":"2003","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0160","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1586\/erv.12.12","article-title":"Recent progress toward an enterotoxigenic E. coli vaccine","author":"Svennerholm","year":"2012","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.molimm.2015.10.016_bib0165","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1586\/14760584.7.6.795","article-title":"Vaccines against enterotoxigenic E. coli","author":"Svennerholm","year":"2008","journal-title":"Expert. Rev. Vaccines"},{"key":"10.1016\/j.molimm.2015.10.016_bib0170","first-page":"223","article-title":"Preparation and titration of CsCl-banded adenovirus stocks","author":"Tollefson","year":"2007","journal-title":"Methods Mol. Med."},{"key":"10.1016\/j.molimm.2015.10.016_bib0175","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1089\/hum.2014.007","article-title":"Challenges in manufacturing adenoviral vectors for global vaccine product deployment","author":"Vellinga","year":"2014","journal-title":"Hum. Gene. Ther."},{"key":"10.1016\/j.molimm.2015.10.016_bib0180","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.jconrel.2005.02.007","article-title":"Fimbriae of enterotoxigenic E. coli function as a mucosal carrier for a coupled heterologous antigen","author":"Verdonck","year":"2005","journal-title":"J. Control. Release"},{"key":"10.1016\/j.molimm.2015.10.016_bib0185","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.actatropica.2014.05.001","article-title":"Intranasal immunisation of the recombinant Toxoplasma gondii receptor for activated C kinase 1 partly protects mice against T. gondii infection","author":"Wang","year":"2014","journal-title":"Acta. Trop."},{"key":"10.1016\/j.molimm.2015.10.016_bib0190","doi-asserted-by":"crossref","first-page":"4113","DOI":"10.1016\/j.vaccine.2009.05.088","article-title":"Immunogenicity and protective efficacy of orally or intranasally administered recombinant Lactobacillus casei expressing ETEC K99","author":"Wei","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.molimm.2015.10.016_bib0195","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1007\/s00253-010-2991-7","article-title":"Protection of mice against enterotoxigenic E. coli by immunization with a polyvalent enterotoxin comprising a combination of LTB, STa, and STb","author":"You","year":"2011","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0200","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1128\/CVI.00095-10","article-title":"Genetic fusions of heat-labile toxoid (LT) and heat-stable toxin b (STb) of porcine enterotoxigenic E. coli elicit protective anti-LT and anti-STb antibodies","author":"Zhang","year":"2010","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.molimm.2015.10.016_bib0205","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1586\/erv.12.37","article-title":"Progress and hurdles in the development of vaccines against enterotoxigenic E. coli in humans","author":"Zhang","year":"2012","journal-title":"Expert. Rev. Vaccines"}],"container-title":["Molecular Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016158901530105X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016158901530105X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T11:22:43Z","timestamp":1599823363000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016158901530105X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12]]},"references-count":41,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["S016158901530105X"],"URL":"https:\/\/doi.org\/10.1016\/j.molimm.2015.10.016","relation":{},"ISSN":["0161-5890"],"issn-type":[{"value":"0161-5890","type":"print"}],"subject":[],"published":{"date-parts":[[2015,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Immunogenicity and protective efficacy of a recombinant adenoviral based vaccine expressing heat-stable enterotoxin (STa) and K99 adhesion antigen of enterotoxigenic Escherichia coli in mice","name":"articletitle","label":"Article Title"},{"value":"Molecular Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.molimm.2015.10.016","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}