{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T13:46:12Z","timestamp":1789911972476,"version":"4.0.1"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T00:00:00Z","timestamp":1543622400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, Brazil (CNPq)"},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior, Brazil (CAPES)"},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais, Brazil (FAPEMIG)"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Immunology"],"published-print":{"date-parts":[[2018,12]]},"DOI":"10.1016\/j.molimm.2018.10.019","type":"journal-article","created":{"date-parts":[[2018,11,3]],"date-time":"2018-11-03T03:00:25Z","timestamp":1541214025000},"page":"20-26","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":18,"special_numbering":"C","title":["Identification of a linear B-cell epitope in the catalytic domain of bothropasin, a metalloproteinase from Bothrops jararaca snake venom"],"prefix":"10.1016","volume":"104","author":[{"given":"Denis A.","family":"Molina Molina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Guerra-Duarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayane L.","family":"Naves de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Costal-Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovana Reis de","family":"\u00c1vila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanete T.","family":"Soccol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo A.","family":"Machado-de-\u00c1vila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Ch\u00e1vez-Ol\u00f3rtegui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"17","key":"10.1016\/j.molimm.2018.10.019_bib0005","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molimm.2018.10.019_bib0010","doi-asserted-by":"crossref","first-page":"5101","DOI":"10.1111\/j.1742-4658.2005.04945.x","article-title":"Protein database searches using compositionally adjusted substitution matrices","volume":"272","author":"Altschul","year":"2005","journal-title":"FEBS J."},{"key":"10.1016\/j.molimm.2018.10.019_bib0015","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.toxicon.2008.12.004","article-title":"Antigenic, microbicidal and antiparasitic properties of an l-amino acid oxidase isolated from Bothrops jararaca snake venom","volume":"53","author":"Ciscotto","year":"2009","journal-title":"Toxicon"},{"key":"10.1016\/j.molimm.2018.10.019_bib0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.jprot.2009.01.020","article-title":"Antidotes against venomous animals: state of the art and prospectives","author":"Espino-Solis","year":"2009","journal-title":"J. Proteomics"},{"key":"10.1016\/j.molimm.2018.10.019_bib0025","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.toxicon.2016.09.010","article-title":"Neutralization of toxicological activities of medically-relevant Bothrops snake venoms and relevant toxins by two polyvalent bothropic antivenoms produced in Peru and Brazil","volume":"122","author":"Estevao-Costa","year":"2016","journal-title":"Toxicon"},{"key":"10.1016\/j.molimm.2018.10.019_bib0030","doi-asserted-by":"crossref","first-page":"4201","DOI":"10.1016\/j.vaccine.2009.04.038","article-title":"An in vivo protective response against toxic effects of the dermonecrotic protein from Loxosceles intermedia spider venom elicited by synthetic epitopes","volume":"27","author":"Felicori","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.molimm.2018.10.019_bib0035","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1016\/j.toxicon.2010.07.014","article-title":"Immunochemical and biological characterization of monoclonal antibodies against BaP1, a metalloproteinase from Bothrops asper snake venom","volume":"56","author":"Fernandes","year":"2010","journal-title":"Toxicon"},{"key":"10.1016\/j.molimm.2018.10.019_bib0040","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1016\/j.toxicon.2006.07.040","article-title":"Antibodies against synthetic epitopes inhibit the enzymatic activity of mutalysin II, a metalloproteinase from bushmaster snake venom","volume":"48","author":"Ferreira","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.molimm.2018.10.019_bib0045","doi-asserted-by":"crossref","DOI":"10.3390\/toxins9080239","article-title":"Insights into the mechanisms involved in strong hemorrhage and dermonecrosis induced by atroxlysin-Ia, a PI-class snake venom metalloproteinase","volume":"9","author":"Freitas-de-Sousa","year":"2017","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.molimm.2018.10.019_bib0050","first-page":"315","article-title":"Automated multiple peptide synthesis","volume":"5","author":"Gausepohl","year":"1992","journal-title":"Pept. Res."},{"key":"10.1016\/j.molimm.2018.10.019_bib0055","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1016\/j.jprot.2011.06.019","article-title":"Research strategies to improve snakebite treatment: challenges and progress","volume":"74","author":"Harrison","year":"2011","journal-title":"J. Proteomics"},{"key":"10.1016\/j.molimm.2018.10.019_bib0060","doi-asserted-by":"crossref","first-page":"4544","DOI":"10.1111\/j.1742-4658.2011.08115.x","article-title":"Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis","volume":"278","author":"Kang","year":"2011","journal-title":"FEBS J."},{"key":"10.1016\/j.molimm.2018.10.019_bib0065","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0022-1759(02)00140-0","article-title":"Application of the Spot method to the identification of peptides and amino acids from the antibody paratope that contribute to antigen binding","volume":"267","author":"Laune","year":"2002","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.molimm.2018.10.019_bib0070","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.toxicon.2018.03.004","article-title":"Pros and cons of different therapeutic antibody formats for recombinant antivenom development","volume":"146","author":"Laustsen","year":"2018","journal-title":"Toxicon"},{"key":"10.1016\/j.molimm.2018.10.019_bib0075","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2018.00653","article-title":"Recombinant protein containing B-cell epitopes of different Loxosceles spider toxins generates neutralizing antibodies in immunized rabbits","volume":"9","author":"Lima","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.molimm.2018.10.019_bib0080","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.toxicon.2004.04.015","article-title":"Molecular characterization of protective antibodies raised in mice by Tityus serrulatus scorpion venom toxins conjugated to bovine serum albumin","volume":"44","author":"Machado De Avila","year":"2004","journal-title":"Toxicon"},{"key":"10.1016\/j.molimm.2018.10.019_bib0085","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1016\/j.peptides.2011.06.028","article-title":"Mimotopes of mutalysin-II from Lachesis muta snake venom induce hemorrhage inhibitory antibodies upon vaccination of rabbits","volume":"32","author":"Machado De Avila","year":"2011","journal-title":"Peptides"},{"key":"10.1016\/j.molimm.2018.10.019_bib0090","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/0041-0101(82)90098-8","article-title":"Isolation and characterization of a proteolytic enzyme from the venom of the snake Bothrops jararaca (Jararaca)","volume":"20","author":"Mandelbaum","year":"1982","journal-title":"Toxicon"},{"key":"10.1016\/j.molimm.2018.10.019_bib0095","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1016\/j.vaccine.2013.03.048","article-title":"Generation and characterization of a recombinant chimeric protein (rCpLi) consisting of B-cell epitopes of a dermonecrotic protein from Loxosceles intermedia spider venom","volume":"31","author":"Mendes","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.molimm.2018.10.019_bib0100","series-title":". Guia de Vigil\u00e2ncia Epidemiol\u00f3gica, S\u00e9rie A. Normas e Manuais T\u00e9cnicos","author":"Minist\u00e9rio da Sa\u00fade","year":"2009"},{"key":"10.1016\/j.molimm.2018.10.019_bib0105","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.toxicon.2007.10.009","article-title":"Neutralization of hemorrhagic snake venom metalloproteinase HR1a from protobothrops flavoviridis by human monoclonal antibody","volume":"51","author":"Morine","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.molimm.2018.10.019_bib0110","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/j.toxicon.2008.08.021","article-title":"The three-dimensional structure of bothropasin, the main hemorrhagic factor from Bothrops jararaca venom: insights for a new classification of snake venom metalloprotease subgroups","volume":"52","author":"Muniz","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.molimm.2018.10.019_bib0115","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.4269\/ajtmh.1985.34.1167","article-title":"Isolation and characterization of hemorrhagic toxin g from the venom of Crotalus atrox (western diamondback rattlesnake)","volume":"34","author":"Nikai","year":"1985","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.molimm.2018.10.019_bib0120","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1160\/TH09-12-0855","article-title":"New insights into the structural elements involved in the skin haemorrhage induced by snake venom metalloproteinases","volume":"104","author":"Oliveira","year":"2010","journal-title":"Thromb. Haemost."},{"key":"10.1016\/j.molimm.2018.10.019_bib0125","doi-asserted-by":"crossref","first-page":"D343","DOI":"10.1093\/nar\/gkv1118","article-title":"Twenty years of the MEROPS database of proteolytic enzymes, their substrates and inhibitors","volume":"44","author":"Rawlings","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.molimm.2018.10.019_bib0130","doi-asserted-by":"crossref","first-page":"1680","DOI":"10.1016\/j.vaccine.2016.02.035","article-title":"Identification of protective B-cell epitopes of Atroxlysin-I: a metalloproteinase from Bothrops atrox snake venom","volume":"34","author":"Schneider","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.molimm.2018.10.019_bib0135","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.toxicon.2017.07.009","article-title":"Peptides derived from plasma proteins released by bothropasin, a metalloprotease present in the Bothrops jararaca venom","volume":"137","author":"Silva","year":"2017","journal-title":"Toxicon"},{"key":"10.1016\/j.molimm.2018.10.019_bib0140","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.toxicon.2004.12.006","article-title":"The improvement of the therapeutic anti-Lachesis muta serum production in horses","volume":"45","author":"Stephano","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.molimm.2018.10.019_bib0145","doi-asserted-by":"crossref","first-page":"0832","DOI":"10.1371\/journal.pmed.0030184","article-title":"Bioinformatics and multiepitope DNA immunization to design rational snake antivenom","volume":"3","author":"Wagstaff","year":"2006","journal-title":"PLoS Med."},{"key":"10.1016\/j.molimm.2018.10.019_bib0150","series-title":"The Venomous Reptiles of the Western Hemisfephere","article-title":"Snakebites in Central and South America: epidemiology, clinical features, and clinical management","author":"Warrell","year":"2004"},{"key":"10.1016\/j.molimm.2018.10.019_bib0155","series-title":"Post ECBS Version Expert Committee on Biological Standardization Geneva, 17 to 21 October 2016 WHO Guidelines for the Production, Control and Regulation of Snake Antivenom Immunoglobulins","first-page":"1","author":"WHO","year":"2016"}],"container-title":["Molecular Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0161589018307296?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0161589018307296?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,11,24]],"date-time":"2018-11-24T04:09:51Z","timestamp":1543032591000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0161589018307296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12]]},"references-count":31,"alternative-id":["S0161589018307296"],"URL":"https:\/\/doi.org\/10.1016\/j.molimm.2018.10.019","relation":{},"ISSN":["0161-5890"],"issn-type":[{"value":"0161-5890","type":"print"}],"subject":[],"published":{"date-parts":[[2018,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification of a linear B-cell epitope in the catalytic domain of bothropasin, a metalloproteinase from Bothrops jararaca snake venom","name":"articletitle","label":"Article Title"},{"value":"Molecular Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.molimm.2018.10.019","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}