{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T16:09:54Z","timestamp":1764432594465},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,6,20]],"date-time":"2007-06-20T00:00:00Z","timestamp":1182297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gap junctions are plasma membrane channels between neighboring cells. We previously described a powerful technique where gap junctional, intercellular communication (GJIC) of adherent cells can be examined by in situ electroporation on a slide, part of which is coated with electrically conductive and transparent indium\u2010tin oxide. An electric pulse is applied through an electrode placed on the cells in the presence of the tracking dye, Lucifer yellow (LY). The pulse causes LY's penetration into the cells growing on the conductive part of the slide, and the subsequent migration of the dye to the non\u2010electroporated cells growing on the non\u2010conductive area is microscopically observed under fluorescence illumination. Although this technique is adequate for a number of cell lines, the turbulence generated as the electrode is removed can cause cell detachment, which makes GJIC examination problematic. In this communication, we describe a slide configuration where junctional communication can be examined in the absence of an upper electrode: Cells are grown on two co\u2010planar electrodes separated by a barrier which diverts the electric field, rendering it vertical to the cell layer. The elimination of an upper electrode is especially valuable for the electroporation of sensitive cells, such as terminally differentiated adipocytes. This technique can also be used for the introduction of other non\u2010permeant molecules such as peptides or siRNA, followed by examination of the cellular phenotype or gene expression levels in situ.<\/jats:p>","DOI":"10.1016\/j.molonc.2007.06.002","type":"journal-article","created":{"date-parts":[[2007,6,21]],"date-time":"2007-06-21T11:08:39Z","timestamp":1182424119000},"page":"226-231","source":"Crossref","is-referenced-by-count":14,"title":["Examination of gap junctional, intercellular communication by <i>in situ<\/i> electroporation on two co\u2010planar indium\u2010tin oxide electrodes"],"prefix":"10.1002","volume":"1","author":[{"given":"Aikaterini","family":"Anagnostopoulou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adina","family":"Vultur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Firth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leda","family":"Raptis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2005.10.047"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.100.1.265"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(90)91210-J"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1996.15.443"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(96)04187-0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1998.17.265"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(87)90014-0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(93)81531-5"},{"key":"e_1_2_6_10_1","first-page":"490","article-title":"A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer","volume":"18","author":"Goldberg G.S.","year":"1995","journal-title":"Biotechniques"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1994.13.963"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1997.3646"},{"key":"e_1_2_6_13_1","first-page":"11","article-title":"Rasleu61 blocks differentiation of transformable 3T3 L1 and C3HT1\/2-derived preadipocytes in a dose- and time-dependent manner","volume":"8","author":"Raptis L.","year":"1997","journal-title":"Cell Growth Differ"},{"key":"e_1_2_6_14_1","first-page":"293","article-title":"Specific inhibition of growth factor-stimulated ERK1\/2 activation in intact cells by electroporation of a Grb2-SH2 binding peptide","volume":"11","author":"Raptis L.","year":"2000","journal-title":"Cell Growth Differ"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.2144\/00292bm04"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012164730-8\/50116-7"},{"key":"e_1_2_6_17_1","first-page":"639","article-title":"A functional assay for intercellular, junctional communication in cultured human lung carcinoma cells","volume":"78","author":"Tomai E.","year":"1998","journal-title":"Lab. Invest."},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-5002(99)00016-1"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2005.08.012"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/104454903321112479"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3961495"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2000.4919"}],"container-title":["Molecular Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.molonc.2007.06.002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.molonc.2007.06.002","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/api.elsevier.com\/content\/article\/PII:S1574789107000415?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/api.elsevier.com\/content\/article\/PII:S1574789107000415?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.molonc.2007.06.002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T22:11:59Z","timestamp":1695507119000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.molonc.2007.06.002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,20]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1016\/j.molonc.2007.06.002"],"URL":"https:\/\/doi.org\/10.1016\/j.molonc.2007.06.002","archive":["Portico"],"relation":{},"ISSN":["1574-7891","1878-0261"],"issn-type":[{"value":"1574-7891","type":"print"},{"value":"1878-0261","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,20]]}}}