{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:51:01Z","timestamp":1791355861322,"version":"4.3.1"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,4,25]],"date-time":"2013-04-25T00:00:00Z","timestamp":1366848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"German Jos\u00e9 Carreras Leukemia Foundation","award":["R06\/22"],"award-info":[{"award-number":["R06\/22"]}]},{"name":"German Jos\u00e9 Carreras Leukemia Foundation","award":["R10\/37f"],"award-info":[{"award-number":["R10\/37f"]}]},{"DOI":"10.13039\/501100005972","name":"Deutsche Krebshilfe","doi-asserted-by":"crossref","award":["10-1988-Bu1"],"award-info":[{"award-number":["10-1988-Bu1"]}],"id":[{"id":"10.13039\/501100005972","id-type":"DOI","asserted-by":"crossref"}]},{"name":"DFG","award":["2453\/1-1"],"award-info":[{"award-number":["2453\/1-1"]}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p>The chromosomal translocation t(8;14)(q24;q32) with juxtaposition of MYC to enhancer elements in the immunoglobulin heavy chain (IGH) gene locus is the genetic hallmark of the majority of Burkitt lymphoma and a subset of Diffuse large B\u2010cell lymphoma patients. Around 3% of adult B\u2010lineage acute lymphoblastic leukemia (ALL) patients show this aberration. Flow cytometry mostly reveals a \u201cmature B\u2010ALL\u201d or \u201cBurkitt\u2010type\u201d ALL immunophenotype. Using long\u2010distance PCR for t(8;14)\/MYC\u2010IGH fusion, we investigated bone marrow, peripheral blood and a few other samples with suspected Burkitt\u2010ALL or mature B\u2010ALL and identified 133 MYC\u2010IGH\u2010positive cases. The location of the chromosomal breaks in the IGH joining and the 8 different switch regions was determined using a set of long\u2010distance PCRs. The chromosomal breakpoints with the adjacent MYC regions on 8q24 were characterized by direct sequencing in 49 cases. The distribution of chromosomal breaks among the IGH joining and switch regions was the following: JH 23.3%, M 21.8%, G1 15.0%, G2 7.5%, G3 3.8%, G4 4.5%, A1 12.8%, A2 3.8%, E 7.5%. Two breakpoint clusters near MYC were delineated. There was no clear correlation between the degree of somatic hypermutation and the chromosomal break locations. Epstein Barr virus was detected in 5 cases (4%). This detailed and extensive molecular analysis illustrates the molecular complexity of the MYC\u2010IGH translocations and the detected distribution of breakpoints provides additional evidence that this translocation results from failed switch and VDJ recombinations. This study may serve as a model for the analysis of other IGH translocations in B\u2010cell lymphoma.<\/jats:p>","DOI":"10.1016\/j.molonc.2013.04.006","type":"journal-article","created":{"date-parts":[[2013,4,25]],"date-time":"2013-04-25T18:03:41Z","timestamp":1366913021000},"page":"850-858","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Erroneous class switching and false VDJ recombination: Molecular dissection of t(8;14)\/MYC\u2010IGH translocations in\u00a0Burkitt\u2010type lymphoblastic leukemia\/B\u2010cell lymphoma"],"prefix":"10.1002","volume":"7","author":[{"given":"Thomas","family":"Burmeister","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mara","family":"Molkentin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Schwartz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"G\u00f6kbuget","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"Hoelzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eckhard","family":"Thiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Reinhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,4,25]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"759","article-title":"Ongoing mutations in the N-terminal domain of c-Myc affect transactivation in Burkitt's lymphoma cell lines","volume":"9","author":"Albert T.","year":"1994","journal-title":"Oncogene"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2003.00278.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65463-6"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-01-0168"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/45.2.309"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403847"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404753"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/8.1.115"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2004.02084.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa055759"},{"key":"e_1_2_8_12_1","first-page":"181","article-title":"Burkitt lymphoma","author":"Diebold J.","year":"2001","journal-title":"Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208746"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08633"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-02-412791"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.09007.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03845"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.2.495.bloodjournal872495"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa055351"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2006.05.017"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204640"},{"key":"e_1_2_8_22_1","unstructured":"2001 Academic Press London M.-P. 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