{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:27:31Z","timestamp":1791073651188,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,8,19]],"date-time":"2013-08-19T00:00:00Z","timestamp":1376870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:p>Targeting tumor marker genes by RNA trans\u2010splicing is a promising means to induce tumor cell\u2010specific death. Using a screening system we designed RNA trans\u2010splicing molecules (RTM) specifically binding the pre\u2010mRNA of SLCO1B3, a marker gene in epidermolysis bullosa associated squamous cell carcinoma (EB\u2010SCC). Specific trans\u2010splicing, results in the fusion of the endogenous target mRNA of SLCO1B3 and the coding sequence of the suicide gene, provided by the RTM. SLCO1B3\u2010specific RTMs containing HSV\u2010tk were analyzed regarding their trans\u2010splicing potential in a heterologous context using a SLCO1B3 expressing minigene (SLCO1B3\u2010MG). Expression of the chimeric SLCO1B3\u2010tk was detected by semi\u2010quantitative RT\u2010PCR and Western blot analysis. Cell viability and apoptosis assays confirmed that the RTMs induced suicide gene\u2010mediated apoptosis in SLCO1B3\u2010MG expressing cells. The lead RTM also showed its potential to facilitate a trans\u2010splicing reaction into the endogenous SLCO1B3 pre\u2010mRNA in EB\u2010SCC cells resulting in tk\u2010mediated apoptosis. We assume that the pre\u2010selection of RTMs by our inducible cell\u2010death system accelerates the design of optimal RTMs capable to induce tumor specific cell death in skin cancer cells.<\/jats:p>","DOI":"10.1016\/j.molonc.2013.08.005","type":"journal-article","created":{"date-parts":[[2013,8,19]],"date-time":"2013-08-19T18:20:31Z","timestamp":1376936431000},"page":"1056-1068","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The design and optimization of RNA <i>trans<\/i>\u2010splicing molecules for skin cancer therapy"],"prefix":"10.1002","volume":"7","author":[{"given":"Christina","family":"Gruber","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Koller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva M.","family":"Murauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Hainzl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemens","family":"H\u00fcttner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Kocher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew P.","family":"South","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut","family":"Hintner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johann W.","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,8,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.04.023"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt108"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-227-8_30"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm900"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4489-09.2010"},{"key":"e_1_2_8_7_1","first-page":"S55","article-title":"SLCO1B3 expression in recessive dystrophic epidermolysis bullosa associated squamous cell carcinoma is reduced by recombinant type VII collagen","volume":"129","author":"Cole C.L.","year":"2009","journal-title":"J.\u00a0Invest Dermatol."},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0625.2003.120105.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5716"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566523033347426"},{"key":"e_1_2_8_11_1","first-page":"5274","article-title":"The \u201cbystander effect\u201d: tumor regression when a fraction of the tumor mass is genetically modified","volume":"53","author":"Freeman S.M.","year":"1993","journal-title":"Cancer Res."},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-10-0669"},{"key":"e_1_2_8_13_1","first-page":"2697","article-title":"Herpes simplex virus thymidine kinase\/ganciclovir-mediated apoptotic death of bystander cells","volume":"56","author":"Hamel W.","year":"1996","journal-title":"Cancer Res."},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-010-8941-0"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2004.08.002"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00430.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/104303401750298580"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr465"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-1984"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2005.16.1116"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.249"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/hgtb.2012.180"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2007.00570.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.01.115"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.254.65.1"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mt.6300096"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.2217\/14622416.8.7.787"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200940221"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/6986"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(75)80001-8"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/cgt.2011.60"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2008.173"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)00731-7"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq405"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.101"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.260"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.180"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/cgt.2009.19"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.09.008"}],"container-title":["Molecular Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.molonc.2013.08.005","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.molonc.2013.08.005","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/api.elsevier.com\/content\/article\/PII:S1574789113001166?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/api.elsevier.com\/content\/article\/PII:S1574789113001166?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.molonc.2013.08.005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T22:01:58Z","timestamp":1695506518000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.molonc.2013.08.005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,19]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["10.1016\/j.molonc.2013.08.005"],"URL":"https:\/\/doi.org\/10.1016\/j.molonc.2013.08.005","archive":["Portico"],"relation":{},"ISSN":["1574-7891","1878-0261"],"issn-type":[{"value":"1574-7891","type":"print"},{"value":"1878-0261","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,19]]},"assertion":[{"value":"2013-07-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}