{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T10:44:40Z","timestamp":1789209880665,"version":"build-2803163510"},"reference-count":56,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T00:00:00Z","timestamp":1474329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Identification of specific risk groups for recurrence after surgery for isolated colorectal liver metastases (CRLM) remains challenging due to the heterogeneity of the disease. Classical clinicopathologic parameters have limited prognostic value. The aim of this study was to identify a gene expression signature measured in CRLM discriminating early from late recurrence after partial hepatectomy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>CRLM from two patient groups were collected: I) with recurrent disease \u226412 months after surgery (N\u00a0=\u00a033), and II) without recurrences and disease free for \u226536 months (N\u00a0=\u00a030). The patients were clinically homogeneous; all had a low clinical risk score (0\u20132) and did not receive (neo\u2010) adjuvant chemotherapy. Total RNA was hybridised to Illumina arrays, and processed for analysis. A leave\u2010one\u2010out cross validation (LOOCV) analysis was performed to identify a prognostic gene expression signature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>LOOCV yielded an 11\u2010gene profile with prognostic value in relation to recurrent disease \u226412 months after partial hepatectomy. This signature had a sensitivity of 81.8%, with a specificity of 66.7% for predicting recurrences (\u226412 months) versus no recurrences for at least 36 months after surgery (X2 P\u00a0&lt;\u00a00.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The current study yielded an 11\u2010gene signature at mRNA level in CRLM discriminating early from late or no relapse after partial hepatectomy.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.molonc.2016.09.002","type":"journal-article","created":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T05:01:01Z","timestamp":1474347661000},"page":"1542-1550","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["mRNA expression profiles of colorectal liver metastases as a novel biomarker for early recurrence after partial hepatectomy"],"prefix":"10.1002","volume":"10","author":[{"given":"E.P.","family":"van der Stok","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Smid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.M.","family":"Sieuwerts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.B.","family":"Vermeulen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sleijfer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Ayez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.J.","family":"Gr\u00fcnhagen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.W.M.","family":"Martens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Verhoef","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(09)70314-6"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-011-2114-4"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejso.2015.04.012"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-015-1199-8"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-15-1071"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.23424"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4212"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-015-4370-1"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-010-1409-1"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3181b4539b"},{"issue":"11","key":"e_1_2_10_12_1","first-page":"490","article-title":"[Improvement of 5 year survival rate after liver resection for colorectal metastases between 1984\u20132006]","volume":"153","author":"Dols L.F.","year":"2009","journal-title":"Verbetering in 5-jaarsoverleving na leverresectie voor colorectale metastasen tussen 1984\u20132006. Ned Tijdschr Geneeskd"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn224"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-015-9715-4"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2167504"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199909000-00004"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.096156"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3967"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0804525"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081680"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.28347"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199912303412702"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2008.01845.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjq039"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0105747"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30089"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004320000199"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamasurg.2015.0313"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3261"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.20344\/amp.4816"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27987"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2015.01.001"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0144097"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00534-002-0778-7"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2014.4"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19960401)77:7<1254::AID-CNCR5>3.0.CO;2-I"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20000715)89:2<267::AID-CNCR10>3.0.CO;2-1"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa041588"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2015.12.015"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjsurg.2013.05.015"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soc.2008.02.009"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.17.6453"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6605659"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-14-174"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.2217\/epi.14.14"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e31815aa2c2"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3175"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0049442"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006225"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3971"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21262"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-012-9469-1"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-12-3931"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-1318.2010.02539.x"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3182a5025a"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.966"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3180603039"}],"container-title":["Molecular Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.molonc.2016.09.002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.molonc.2016.09.002","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/api.elsevier.com\/content\/article\/PII:S1574789116301016?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/api.elsevier.com\/content\/article\/PII:S1574789116301016?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.molonc.2016.09.002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T23:30:41Z","timestamp":1695511841000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.molonc.2016.09.002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,20]]},"references-count":56,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1016\/j.molonc.2016.09.002"],"URL":"https:\/\/doi.org\/10.1016\/j.molonc.2016.09.002","archive":["Portico"],"relation":{},"ISSN":["1574-7891","1878-0261"],"issn-type":[{"value":"1574-7891","type":"print"},{"value":"1878-0261","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,20]]},"assertion":[{"value":"2016-06-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-09-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-09-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}