{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:03:06Z","timestamp":1789383786786,"version":"build-2803163510"},"reference-count":49,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Secretariat of Science Humanities Technology and Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013704","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda, Paraguay","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013704","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006087","name":"National Autonomous University of Mexico Directorate General of Academic Staff Affairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["molpharm.aspetjournals.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Pharmacology"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.molpha.2026.100143","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T15:14:52Z","timestamp":1784906092000},"page":"100143","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Roles of the phosphorylation sites of distinct \u03b11A-adrenergic receptor domains"],"prefix":"10.1016","volume":"108","author":[{"given":"Roc\u00edo","family":"Alc\u00e1ntara-Hern\u00e1ndez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Hern\u00e1ndez-Espinosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Garc\u00eda-Merino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5235-0657","authenticated-orcid":false,"given":"J. Adolfo","family":"Garc\u00eda-S\u00e1inz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.molpha.2026.100143_bib1","series-title":"Goodman and Gilman\u015b the Pharmacological Basis of Therapeutics","first-page":"31","article-title":"Pharmacodynamics: molecular mechanisms of drug action","author":"Blumenthal","year":"2018"},{"issue":"1","key":"10.1016\/j.molpha.2026.100143_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0014-2999(99)00896-1","article-title":"Alpha 1-adrenoceptors: function and phosphorylation","volume":"389","author":"Garc\u00eda-S\u00e1inz","year":"2000","journal-title":"Eur J Pharmacol"},{"issue":"14","key":"10.1016\/j.molpha.2026.100143_bib3","doi-asserted-by":"crossref","first-page":"2343","DOI":"10.1111\/bph.14617","article-title":"Updates in the function and regulation of \u03b11-adrenoceptors","volume":"176","author":"Akinaga","year":"2019","journal-title":"Br J Pharmacol"},{"issue":"6","key":"10.1016\/j.molpha.2026.100143_bib4","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/S0898-6568(02)00151-1","article-title":"G protein-coupled receptor cross-talk: pivotal roles of protein phosphorylation and protein-protein interactions","volume":"15","author":"V\u00e1zquez-Prado","year":"2003","journal-title":"Cell Signal"},{"issue":"3","key":"10.1016\/j.molpha.2026.100143_bib5","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1124\/molpharm.121.000429","article-title":"Roles of receptor phosphorylation and Rab proteins in G protein-coupled receptor function and trafficking","volume":"101","author":"Mart\u00ednez-Morales","year":"2022","journal-title":"Mol Pharmacol"},{"issue":"5","key":"10.1016\/j.molpha.2026.100143_bib6","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.arcmed.2022.06.008","article-title":"Cell trafficking and function of G protein-coupled receptors","volume":"53","author":"Mart\u00ednez-Morales","year":"2022","journal-title":"Arch Med Res"},{"key":"10.1016\/j.molpha.2026.100143_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.pharmthera.2025.108877","article-title":"The many facets of biased signaling: mechanisms and possible therapeutic implications","volume":"272","author":"Solis","year":"2025","journal-title":"Pharmacol Ther"},{"issue":"8","key":"10.1016\/j.molpha.2026.100143_bib8","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.tips.2008.05.006","article-title":"Location, location, location\u2026site-specific GPCR phosphorylation offers a mechanism for cell-type-specific signalling","volume":"29","author":"Tobin","year":"2008","journal-title":"Trends Pharmacol Sci"},{"issue":"5","key":"10.1016\/j.molpha.2026.100143_bib9","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1124\/mol.115.101949","article-title":"Distinct phosphorylation clusters determine the signaling outcome of free fatty acid receptor 4\/G protein-coupled receptor 120","volume":"89","author":"Prihandoko","year":"2016","journal-title":"Mol Pharmacol"},{"issue":"185","key":"10.1016\/j.molpha.2026.100143_bib10","doi-asserted-by":"crossref","DOI":"10.1126\/scisignal.2001707","article-title":"Distinct phosphorylation sites on the \u03b2(2)-adrenergic receptor establish a barcode that encodes differential functions of \u03b2-arrestin","volume":"4","author":"Nobles","year":"2011","journal-title":"Sci Signal"},{"issue":"1","key":"10.1016\/j.molpha.2026.100143_bib11","doi-asserted-by":"crossref","DOI":"10.1038\/srep22495","article-title":"Distinct phosphorylation sites on the ghrelin receptor, GHSR1a, establish a code that determines the functions of \u00df-arrestins","volume":"6","author":"Bouzo-Lorenzo","year":"2016","journal-title":"Sci Rep"},{"issue":"9","key":"10.1016\/j.molpha.2026.100143_bib12","doi-asserted-by":"crossref","first-page":"6553","DOI":"10.1074\/jbc.275.9.6553","article-title":"Norepinephrine- and phorbol ester-induced phosphorylation of \u03b1(1a)-adrenergic receptors. Functional aspects","volume":"275","author":"V\u00e1zquez-Prado","year":"2000","journal-title":"J Biol Chem"},{"issue":"5\u20136","key":"10.1016\/j.molpha.2026.100143_bib13","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/s00210-010-0569-7","article-title":"Roles of the \u03b11A-adrenergic receptor carboxyl tail in protein kinase C-induced phosphorylation and desensitization","volume":"382","author":"Cabrera-Wrooman","year":"2010","journal-title":"Naunyn Schmiedeberg Arch Pharmacol"},{"issue":"11","key":"10.1016\/j.molpha.2026.100143_bib14","doi-asserted-by":"crossref","first-page":"9570","DOI":"10.1074\/jbc.M111762200","article-title":"Acute agonist-mediated desensitization of the human \u03b11a-adrenergic receptor is primarily independent of carboxyl terminus regulation: implications for regulation of \u03b11aAR splice variants","volume":"277","author":"Price","year":"2002","journal-title":"J Biol Chem"},{"issue":"1","key":"10.1016\/j.molpha.2026.100143_bib15","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0026-895X(25)09135-7","article-title":"Effect of phorbol myristate acetate on alpha 1-adrenergic action in cells expressing recombinant alpha 1-adrenoceptor subtypes","volume":"50","author":"V\u00e1zquez-Prado","year":"1996","journal-title":"Mol Pharmacol"},{"issue":"6","key":"10.1016\/j.molpha.2026.100143_bib16","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1016\/j.bbamcr.2018.03.006","article-title":"Different phosphorylation patterns regulate \u03b11D-adrenoceptor signaling and desensitization","volume":"1865","author":"Alfonzo-M\u00e9ndez","year":"2018","journal-title":"Biochim Biophys Acta"},{"issue":"12","key":"10.1016\/j.molpha.2026.100143_bib17","doi-asserted-by":"crossref","first-page":"2378","DOI":"10.1016\/j.bbamcr.2017.09.002","article-title":"Noradrenaline, oxymetazoline and phorbol myristate acetate induce distinct functional actions and phosphorylation patterns of \u03b11A-adrenergic receptors","volume":"1864","author":"Alc\u00e1ntara-Hern\u00e1ndez","year":"2017","journal-title":"Biochim Biophys Acta"},{"issue":"10","key":"10.1016\/j.molpha.2026.100143_bib18","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1016\/j.bbamcr.2019.07.006","article-title":"Sites phosphorylated in human \u03b11B-adrenoceptors in response to noradrenaline and phorbol myristate acetate","volume":"1866","author":"Hern\u00e1ndez-Espinosa","year":"2019","journal-title":"Biochim Biophys Acta"},{"issue":"23","key":"10.1016\/j.molpha.2026.100143_bib19","doi-asserted-by":"crossref","DOI":"10.3390\/ijms242316963","article-title":"Roles of the \u03b1(1B)-adrenergic receptor phosphorylation domains in signaling and internalization","volume":"24","author":"Hern\u00e1ndez-Espinosa","year":"2023","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.molpha.2026.100143_bib20","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.cellsig.2018.11.003","article-title":"Distinct phosphorylation sites\/clusters in the carboxyl terminus regulate \u03b11D-adrenergic receptor subcellular localization and signaling","volume":"53","author":"Carmona-Rosas","year":"2019","journal-title":"Cell Signal"},{"key":"10.1016\/j.molpha.2026.100143_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejphar.2019.172846","article-title":"Roles of the G protein-coupled receptor kinase 2 and Rab5 in \u03b11B-adrenergic receptor function and internalization","volume":"867","author":"Hern\u00e1ndez-Espinosa","year":"2020","journal-title":"Eur J Pharmacol"},{"key":"10.1016\/j.molpha.2026.100143_bib22","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/978-1-61779-126-0_2","article-title":"Using the Flp-In T-Rex system to regulate GPCR expression","volume":"746","author":"Ward","year":"2011","journal-title":"Methods Mol Biol"},{"issue":"5259","key":"10.1016\/j.molpha.2026.100143_bib23","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"issue":"6","key":"10.1016\/j.molpha.2026.100143_bib24","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1016\/S0021-9258(19)83641-4","article-title":"A new generation of Ca2+ indicators with greatly improved fluorescence properties","volume":"260","author":"Grynkiewicz","year":"1985","journal-title":"J Biol Chem"},{"key":"10.1016\/j.molpha.2026.100143_bib25","author":"Rasband"},{"issue":"3","key":"10.1016\/j.molpha.2026.100143_bib26","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0121165","article-title":"\u03b11B-adrenergic receptors differentially associate with Rab proteins during homologous and heterologous desensitization","volume":"10","author":"Castillo-Badillo","year":"2015","journal-title":"PLoS One"},{"issue":"10","key":"10.1016\/j.molpha.2026.100143_bib27","doi-asserted-by":"crossref","first-page":"5508","DOI":"10.3390\/ijms25105508","article-title":"LPA3 receptor phosphorylation sites: roles in signaling and internalization","volume":"25","author":"Sol\u00eds","year":"2024","journal-title":"Int J Mol Sci"},{"issue":"1","key":"10.1016\/j.molpha.2026.100143_bib28","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1002\/0471142727.mb1415s102","article-title":"Basic image analysis and manipulation in ImageJ","volume":"102","author":"Hartig","year":"2013","journal-title":"Curr Protoc Mol Biol"},{"issue":"7","key":"10.1016\/j.molpha.2026.100143_bib29","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat Methods"},{"issue":"2","key":"10.1016\/j.molpha.2026.100143_bib30","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1074\/jbc.273.2.685","article-title":"Essential role for G protein-coupled receptor endocytosis in the activation of mitogen-activated protein kinase","volume":"273","author":"Daaka","year":"1998","journal-title":"J Biol Chem"},{"issue":"6966","key":"10.1016\/j.molpha.2026.100143_bib31","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1038\/nature02158","article-title":"Protein kinase C switches the Raf kinase inhibitor from Raf-1 to GRK-2","volume":"426","author":"Lorenz","year":"2003","journal-title":"Nature"},{"issue":"6434","key":"10.1016\/j.molpha.2026.100143_bib32","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1038\/364249a0","article-title":"Protein kinase C alpha activates RAF-1 by direct phosphorylation","volume":"364","author":"Kolch","year":"1993","journal-title":"Nature"},{"issue":"13","key":"10.1016\/j.molpha.2026.100143_bib33","doi-asserted-by":"crossref","first-page":"11506","DOI":"10.1074\/jbc.M110.154526","article-title":"Differential G-protein-coupled receptor phosphorylation provides evidence for a signaling bar code","volume":"286","author":"Butcher","year":"2011","journal-title":"J Biol Chem"},{"issue":"36","key":"10.1016\/j.molpha.2026.100143_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/S0021-9258(17)31728-3","article-title":"Truncation of the receptor carboxyl terminus impairs agonist-dependent phosphorylation and desensitization of the alpha 1B-adrenergic receptor","volume":"269","author":"Lattion","year":"1994","journal-title":"J Biol Chem"},{"issue":"45","key":"10.1016\/j.molpha.2026.100143_bib35","doi-asserted-by":"crossref","first-page":"28712","DOI":"10.1074\/jbc.272.45.28712","article-title":"Characterization of the phosphorylation sites involved in G protein-coupled receptor kinase- and protein kinase C-mediated desensitization of the alpha1B-adrenergic receptor","volume":"272","author":"Diviani","year":"1997","journal-title":"J Biol Chem"},{"issue":"30","key":"10.1016\/j.molpha.2026.100143_bib36","doi-asserted-by":"crossref","first-page":"18677","DOI":"10.1074\/jbc.273.30.18677","article-title":"G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization","volume":"273","author":"Lefkowitz","year":"1998","journal-title":"J Biol Chem"},{"issue":"3","key":"10.1016\/j.molpha.2026.100143_bib37","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1242\/jcs.115.3.455","article-title":"The role of beta-arrestins in the termination and transduction of G-protein-coupled receptor signals","volume":"115","author":"Luttrell","year":"2002","journal-title":"J Cell Sci"},{"issue":"3","key":"10.1016\/j.molpha.2026.100143_bib38","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.pharmthera.2005.09.008","article-title":"The structural basis of arrestin-mediated regulation of G-protein-coupled receptors","volume":"110","author":"Gurevich","year":"2006","journal-title":"Pharmacol Ther"},{"issue":"9","key":"10.1016\/j.molpha.2026.100143_bib39","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.tips.2011.05.002","article-title":"\u03b2-Arrestin-mediated receptor trafficking and signal transduction","volume":"32","author":"Shenoy","year":"2011","journal-title":"Trends Pharmacol Sci"},{"issue":"4","key":"10.1016\/j.molpha.2026.100143_bib40","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/j.cell.2016.07.004","article-title":"GPCR-G protein-\u03b2-arrestin super-complex mediates sustained G protein signaling","volume":"166","author":"Thomsen","year":"2016","journal-title":"Cell"},{"issue":"549","key":"10.1016\/j.molpha.2026.100143_bib41","doi-asserted-by":"crossref","DOI":"10.1126\/scisignal.aav1646","article-title":"Arrestins and G proteins in cellular signaling: the coin has two sides","volume":"11","author":"Gurevich","year":"2018","journal-title":"Sci Signal"},{"issue":"22","key":"10.1016\/j.molpha.2026.100143_bib42","doi-asserted-by":"crossref","first-page":"4413","DOI":"10.1007\/s00018-019-03272-5","article-title":"Plethora of functions packed into 45 kDa arrestins: biological implications and possible therapeutic strategies","volume":"76","author":"Gurevich","year":"2019","journal-title":"Cell Mol Life Sci"},{"key":"10.1016\/j.molpha.2026.100143_bib43","doi-asserted-by":"crossref","first-page":"125","DOI":"10.3389\/fphar.2019.00125","article-title":"GPCR signaling regulation: the role of GRKs and arrestins","volume":"10","author":"Gurevich","year":"2019","journal-title":"Front Pharmacol"},{"key":"10.1016\/j.molpha.2026.100143_bib44","article-title":"\u03b2-Adrenergic receptor signaling mediated by \u03b2-arrestins and its potential role in heart failure","volume":"37","author":"Nibley","year":"2024","journal-title":"Curr Opin Physiol"},{"issue":"3","key":"10.1016\/j.molpha.2026.100143_bib45","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.cell.2017.07.002","article-title":"Identification of phosphorylation codes for arrestin recruitment by G protein-coupled receptors","volume":"170","author":"Zhou","year":"2017","journal-title":"Cell"},{"issue":"5","key":"10.1016\/j.molpha.2026.100143_bib46","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1083\/jcb.200210140","article-title":"Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations","volume":"160","author":"Sekar","year":"2003","journal-title":"J Cell Biol"},{"key":"10.1016\/j.molpha.2026.100143_bib47","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ceb.2018.11.001","article-title":"Localised GPCR signalling as revealed by FRET biosensors","volume":"57","author":"Halls","year":"2019","journal-title":"Curr Opin Cell Biol"},{"issue":"37","key":"10.1016\/j.molpha.2026.100143_bib48","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abb8368","article-title":"Distinct phosphorylation sites in a prototypical GPCR differently orchestrate \u03b2-arrestin interaction, trafficking, and signaling","volume":"6","author":"Dwivedi-Agnihotri","year":"2020","journal-title":"Sci Adv"},{"issue":"29","key":"10.1016\/j.molpha.2026.100143_bib49","doi-asserted-by":"crossref","first-page":"12011","DOI":"10.1073\/pnas.0704849104","article-title":"Functional specialization of beta-arrestin interactions revealed by proteomic analysis","volume":"104","author":"Xiao","year":"2007","journal-title":"Proc Natl Acad Sci USA"}],"container-title":["Molecular Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0026895X2600043X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0026895X2600043X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T10:10:33Z","timestamp":1789380633000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0026895X2600043X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":49,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["S0026895X2600043X"],"URL":"https:\/\/doi.org\/10.1016\/j.molpha.2026.100143","relation":{},"ISSN":["0026-895X"],"issn-type":[{"value":"0026-895X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Roles of the phosphorylation sites of distinct \u03b11A-adrenergic receptor domains","name":"articletitle","label":"Article Title"},{"value":"Molecular Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.molpha.2026.100143","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 American Society for Pharmacology and Experimental Therapeutics. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"100143"}}