{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T02:39:58Z","timestamp":1767148798771,"version":"build-2238731810"},"reference-count":92,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Health and Environmental Sciences Institute. Washington, DC, USA"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Mutation Research\/Genetic Toxicology and Environmental Mutagenesis"],"published-print":{"date-parts":[[2018,8]]},"DOI":"10.1016\/j.mrgentox.2018.05.021","type":"journal-article","created":{"date-parts":[[2018,5,29]],"date-time":"2018-05-29T13:01:37Z","timestamp":1527598897000},"page":"7-18","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"special_numbering":"C","title":["Identifying germ cell mutagens using OECD test guideline 488 (transgenic rodent somatic and germ cell gene mutation assays) and integration with somatic cell testing"],"prefix":"10.1016","volume":"832-833","author":[{"given":"Francesco","family":"Marchetti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilyn J.","family":"Aardema","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol","family":"Beevers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8021-1005","authenticated-orcid":false,"given":"Jan","family":"van Benthem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Godschalk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carole L.","family":"Yauk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Young","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George R.","family":"Douglas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mrgentox.2018.05.021_bib0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.mrgentox.2018.05.020","article-title":"Simulation of mouse and rat spermatogenesis to inform genotoxicity testing using OECD test guideline 488","author":"Marchetti","year":"2018","journal-title":"Mutat. Res.Genet. Toxicol. Environ. Mutagenesis"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0010","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1002\/em.21685","article-title":"Declaring the existence of human germ-cell mutagens","volume":"53","author":"DeMarini","year":"2012","journal-title":"Environ. Mol. Mutagen"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0015","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.mrgentox.2015.01.008","article-title":"Approaches for identifying germ cell mutagens: report of the 2013 IWGT workshop on germ cell assays","volume":"783","author":"Yauk","year":"2015","journal-title":"Mutat. Res. Genet. Toxicol. Environ. Mutagen"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0020","series-title":"Test No. 488: Transgenic Rodent Somatic and Germ Cell Gene Mutation Assay, OECD Guidelines for Testing of Chemicals, Section 4","author":"OECD","year":"2013"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mrrev.2005.04.002","article-title":"Detailed review of transgenic rodent mutation assays","volume":"590","author":"Lambert","year":"2005","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0030","series-title":"Globally Harmonized System of Classification and Labelling of Chemicals (GHS)","doi-asserted-by":"crossref","DOI":"10.18356\/dbde9a22-en","author":"Nations","year":"2013"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0035","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.mrgentox.2003.07.004","article-title":"In vivo transgenic mutation assays","volume":"540","author":"Thybaud","year":"2003","journal-title":"Mutat. Res. Genet. Toxicol. Environ. Mutagenesis"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0040","series-title":"Detailed Review Paper on Transgenic Rodent Mutations Assay Series on Testing and Assessment, Paris","author":"OECD","year":"2009"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0045","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1002\/dvg.20258","article-title":"From primordial germ cell to primordial follicle: mammalian female germ cell development","volume":"44","author":"Pepling","year":"2006","journal-title":"Genesis"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0050","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/em.10131","article-title":"Treatment and sampling protocols for transgenic mutation assays","volume":"41","author":"Heddle","year":"2003","journal-title":"Environ. Mol. Mutagen"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0055","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0027-5107(96)00029-2","article-title":"Mutagenicity of anticancer drugs in mammalian germ cells","volume":"355","author":"Witt","year":"1996","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0060","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1002\/bdrc.20040","article-title":"Mechanisms and consequences of paternally-transmitted chromosomal abnormalities","volume":"75","author":"Marchetti","year":"2005","journal-title":"Birth Defects Res. C Embryo Today"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0065","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1051\/rnd:19840709","article-title":"Autoradiographic investigation of sperm transit through the male mouse genital tract after tritiated thymidine incorporation","volume":"24","author":"Dadoune","year":"1984","journal-title":"Reprod. Nutr. Dev."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0070","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0011349","article-title":"Environmental exposure of the mouse germ line: DNA adducts in spermatozoa and formation of de novo mutations during spermatogenesis","volume":"5","author":"Olsen","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0075","article-title":"Early-life exposure to benzo[a]pyrene increases mutant frequency in spermatogenic cells in adulthood","volume":"9","author":"Xu","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0080","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1093\/toxsci\/kfw088","article-title":"Benzo(a)pyrene Is mutagenic in mouse spermatogonial stem cells and dividing spermatogonia","volume":"152","author":"O\u2019Brien","year":"2016","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0085","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S1383-5718(96)00117-9","article-title":"Toward an understanding of the use of transgenic mice for the detection of gene mutations in germ cells","volume":"388","author":"Douglas","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0090","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S1383-5718(96)00108-8","article-title":"Preliminary results of ethylnitrosourea, isopropyl methanesulphonate and methyl methanesulphonate activity in the testis and epididymal spermatozoa of muta mice","volume":"388","author":"Mattison","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0095","doi-asserted-by":"crossref","first-page":"7485","DOI":"10.1073\/pnas.92.16.7485","article-title":"Temporal and molecular characteristics of mutations induced by ethylnitrosourea in germ cells isolated from seminiferous tubules and in spermatozoa of lacZ transgenic mice","volume":"92","author":"Douglas","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0100","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1093\/mutage\/10.3.209","article-title":"Germ cell mutagenesis in lambda lacZ transgenic mice treated with ethylnitrosourea; Comparison with specific-locus test","volume":"10","author":"van Delft","year":"1995","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0105","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S1383-5718(96)00121-0","article-title":"Mutations induced in male germ cells after treatment of transgenic mice with ethylnitrosourea","volume":"388","author":"Katoh","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0110","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S1383-5718(96)00123-4","article-title":"Sources of variability in data from a positive selection lacZ transgenic mouse mutation assay: an interlaboratory study","volume":"388","author":"Piegorsch","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0115","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1002\/em.21932","article-title":"Sublinear response in lacZ mutant frequency of Muta mouse spermatogonial stem cells after low dose subchronic exposure to N-ethyl-N-nitrosourea","volume":"56","author":"O\u2019Brien","year":"2015","journal-title":"Environ. Mol. Mutagen"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0120","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s41021-016-0035-y","article-title":"Estimation of the frequency of inherited germline mutations by whole exome sequencing in ethyl nitrosourea-treated and untreated gpt delta mice","volume":"38","author":"Masumura","year":"2016","journal-title":"Genes Environ."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0125","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0165-1218(94)90020-5","article-title":"Studies on mutations in male germ cells of transgenic mice following exposure to isopropyl methanesulfonate, ethylnitrosourea or X-ray","volume":"341","author":"Katoh","year":"1994","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0130","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1530\/rep.0.1210347","article-title":"Proliferation and differentiation of spermatogonial stem cells","volume":"121","author":"de Rooij","year":"2001","journal-title":"Reproduction"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0135","doi-asserted-by":"crossref","first-page":"11391","DOI":"10.1073\/pnas.190330997","article-title":"Proliferation is necessary for both repair and mutation in transgenic mouse cells","volume":"97","author":"Bielas","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0140","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1530\/rep.0.1210031","article-title":"DNA repair mechanisms and gametogenesis","volume":"121","author":"Baarends","year":"2001","journal-title":"Reproduction"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0145","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.taap.2005.01.060","article-title":"How do male germ cells handle DNA damage?","volume":"207","author":"Olsen","year":"2005","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0150","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s10709-004-1443-7","article-title":"Effects of male germ-cell stage on the frequency, nature, and spectrum of induced specific-locus mutations in the mouse","volume":"122","author":"Russell","year":"2004","journal-title":"Genetica"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0155","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1093\/mutage\/13.1.51","article-title":"Lack of mutagenicity of levofloxacin in lacZ transgenic mice","volume":"13","author":"Itoh","year":"1998","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0160","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S1383-5718(96)00107-6","article-title":"Mutation assays in male germ cells from transgenic mice: overview of study and conclusions","volume":"388","author":"Ashby","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0165","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S1383-5718(96)00116-7","article-title":"Evaluation of lacI mutation in germ cells and micronuclei in peripheral blood after treatment of male lacI transgenic mice with ethylnitrosourea, isopropylmethane sulfonate or methylmethane sulfonate","volume":"388","author":"Gorelick","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0170","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S1383-5718(96)00111-8","article-title":"Effect of ethylnitrosourea and methyl methanesulfonate on mutation frequency in muta mouse germ cells (seminiferous tubule cells and epididymis spermatozoa)","volume":"388","author":"Renault","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0175","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S1383-5718(96)00112-X","article-title":"Ethyl nitrosourea and methyl methanesulfonate mutagenicity in sperm and testicular germ cells of lacZ transgenic mice (Muta Mouse)","volume":"388","author":"Suzuki","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0180","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1002\/(SICI)1098-2280(1999)34:2\/3<221::AID-EM23>3.0.CO;2-E","article-title":"Effects of O (6)-alkylguanine-DNA alkyltransferase deficiency in Escherichia coli as the host for the detection of mutations in lacI transgenic mice","volume":"34","author":"Sui","year":"1999","journal-title":"Environ. Mol. Mutagen"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0185","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S1383-5718(96)00115-5","article-title":"The activity of ENU, iPMS and MMS in male Mouse germ cells using the Muta Mouse positive selection transgenic mutation assay","volume":"388","author":"Tinwell","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0190","doi-asserted-by":"crossref","DOI":"10.3791\/51576-v","article-title":"Transgenic rodent assay for quantifying male germ cell mutant frequency","author":"O\u2019Brien","year":"2014","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0195","doi-asserted-by":"crossref","first-page":"7958","DOI":"10.1073\/pnas.88.18.7958","article-title":"Spectra of spontaneous and mutagen-induced mutations in the lacI gene in transgenic mice","volume":"88","author":"Kohler","year":"1991","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0200","doi-asserted-by":"crossref","first-page":"6564","DOI":"10.1073\/pnas.91.14.6564","article-title":"Characterization of mutations induced by ethylnitrosourea in seminiferous tubule germ cells of transgenic B6C3F1 mice","volume":"91","author":"Provost","year":"1994","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0205","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S1383-5718(96)00109-X","article-title":"Evaluation of the transgenic Lambda\/LacI mouse model as a short-term predictor of heritable risk","volume":"388","author":"Provost","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0210","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S1383-5718(96)00110-6","article-title":"Evaluation of spontaneous and chemical-induced lacI mutations in germ cells from lambda\/lacI transgenic mice","volume":"388","author":"Putman","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0215","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S1383-5718(96)00114-3","article-title":"Analysis of the mutagenic potential of ENU and MMS in germ cells of male C57BL\/6 lacI transgenic mice","volume":"388","author":"Winegar","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0220","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1093\/toxsci\/kfq190","article-title":"Mutagenicity of acrylamide and glycidamide in the testes of big blue mice","volume":"117","author":"Wang","year":"2010","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0225","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1093\/mutage\/ger014","article-title":"Acrylamide genotoxicity in young versus adult gpt delta male rats","volume":"26","author":"Koyama","year":"2011","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0230","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0050465","article-title":"The effects of Di-(2-ethylhexyl)-phthalate exposure on fertilization and embryonic development in vitro and testicular genomic mutation in vivo","volume":"7","author":"Huang","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0235","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1093\/mutage\/geh017","article-title":"In vivo mutagenicity and mutation spectrum in the bone marrow and testes of B6C3F1 lacI transgenic mice following inhalation exposure to ethylene oxide","volume":"19","author":"Recio","year":"2004","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0240","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1093\/toxsci\/kfq325","article-title":"Evaluation of benzo(a)pyrene-induced gene mutations in male germ cells","volume":"119","author":"Verhofstad","year":"2011","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0245","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/0027-5107(93)90159-D","article-title":"A quantitative study of spermatogonial multiplication and stem cell renewal in the C3H\/101 F1 hybrid mouse","volume":"290","author":"Tegelenbosch","year":"1993","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0250","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0027-5107(95)00223-5","article-title":"Comparison of the duration of spermatogenesis between male rodents and humans","volume":"352","author":"Adler","year":"1996","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0255","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/0014-4827(73)90507-7","article-title":"Cellular composition of fractions of mouse testis cells following velocity sedimentation separation","volume":"79","author":"Meistrich","year":"1973","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0260","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1002\/jcp.1040860119","article-title":"Centrifugal elutriation: separation of spermatogenic cells on the basis of sedimentation velocity","volume":"86","author":"Grabske","year":"1975","journal-title":"J. Cell Physiol"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0265","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/0076-6879(93)25009-Q","article-title":"Purification, culture, and fractionation of spermatogenic cells","volume":"225","author":"Bellve","year":"1993","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0270","doi-asserted-by":"crossref","first-page":"10015","DOI":"10.1073\/pnas.95.17.10015","article-title":"Mutation frequency declines during spermatogenesis in young mice but increases in old mice","volume":"95","author":"Walter","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0275","doi-asserted-by":"crossref","DOI":"10.3791\/2602","article-title":"Flow cytometry purification of mouse meiotic cells","author":"Getun","year":"2011","journal-title":"J. Vis. Exp."},{"issue":"744","key":"10.1016\/j.mrgentox.2018.05.021_bib0280","article-title":"Flow cytometry of murine spermatocytes","volume":"72","author":"Gaysinskaya","year":"2015","journal-title":"Curr. Protoc. Cytom."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0285","series-title":"Test 474: Mammalian Erythrocyte Micronucleus Test, OECD Guidelines for Testing of Chemicals, Section 4","author":"OECD","year":"2016"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0290","first-page":"56274","article-title":"Guidelines for reproductive toxicity risk assessment","volume":"61","author":"EPA","year":"1996","journal-title":"Fed. Regist."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0295","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0378-4274(83)90038-3","article-title":"Testes weight reflect ethylnitrosourea induced histopathology in mice","volume":"17","author":"Rodriguez","year":"1983","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0300","article-title":"Somatic and germ cell mutant analysis in the Big blue\u00ae transgenic mouse mutation assay with N-Ethyl-N-nitrosourea (ENU)","volume":"138","author":"Young","year":"2014","journal-title":"Toxicol.: Suppl. Toxicol. Sci."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0305","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.yrtph.2009.06.001","article-title":"Practical issues on the application of the GHS classification criteria for germ cell mutagens","volume":"55","author":"Morita","year":"2009","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0310","doi-asserted-by":"crossref","first-page":"213","DOI":"10.2486\/indhealth.38.213","article-title":"Examination of lacZ mutant induction in the liver and testis of Muta mouse following injection of halogenated aliphatic hydrocarbons classified as human carcinogens","volume":"38","author":"Hachiya","year":"2000","journal-title":"Ind. Health"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0315","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1093\/mutage\/13.6.607","article-title":"Mutation studies in lacI transgenic mice after exposure to radiation or cyclophosphamide","volume":"13","author":"Hoyes","year":"1998","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0320","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1620\/tjem.202.93","article-title":"Absence of mutagenic effects of 2.45 GHz radiofrequency exposure in spleen, liver, brain, and testis of lacZ-transgenic mouse exposed in utero","volume":"202","author":"Ono","year":"2004","journal-title":"Tohoku J. Exp. Med."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0325","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S1383-5718(98)00029-1","article-title":"Antimutagenic structural modification of quinoline assessed by an in vivo mutagenesis assay using lacZ-transgenic mice","volume":"414","author":"Miyata","year":"1998","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0330","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1002\/em.20014","article-title":"DNA adducts and mutagenic specificity of the ubiquitous environmental pollutant 3-nitrobenzanthrone in Muta Mouse","volume":"43","author":"Arlt","year":"2004","journal-title":"Environ. Mol. Mutagen"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0335","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S1383-5718(99)00105-9","article-title":"Mutagenicity of 4-nitroquinoline 1-oxide in the MutaMouse","volume":"444","author":"Nakajima","year":"1999","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0340","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/S1383-5718(99)00063-7","article-title":"Induction of lacZ mutation by 7,12-dimethylbenz[a]anthracene in various tissues of transgenic mice","volume":"444","author":"Hachiya","year":"1999","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0345","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S1383-5718(00)00103-0","article-title":"Mutagenic properties of a nitrofuran, 7-methoxy-2-nitronaphtho[2, 1-b]furan (R7000), in lacI transgenic mice","volume":"470","author":"Quillardet","year":"2000","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0350","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1093\/toxsci\/kfq069","article-title":"The genotoxicity of acrylamide and glycidamide in big blue rats","volume":"115","author":"Mei","year":"2010","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0355","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S1383-5718(01)00350-3","article-title":"Mutagenicity of aristolochic acid in the lambda\/lacZ transgenic mouse (MutaMouse)","volume":"515","author":"Kohara","year":"2002","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0360","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1093\/mutage\/8.1.7","article-title":"Detection of chemical mutagens using muta mouse: a transgenic mouse model","volume":"8","author":"Hoorn","year":"1993","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0365","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1002\/(SICI)1098-2280(1996)28:4<348::AID-EM8>3.0.CO;2-7","article-title":"Organ variation in the mutagenicity of dimethylnitrosamine in Big Blue mice","volume":"28","author":"Suzuki","year":"1996","journal-title":"Environ. Mol. Mutagen"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0370","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S1383-5718(99)00062-5","article-title":"N-nitrosodi-n-propylamine induces organ specific mutagenesis with specific expression times in lacZ transgenic mice","volume":"444","author":"Itoh","year":"1999","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0375","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S1383-5718(97)00063-6","article-title":"Assessment of the in vivo mutagenicity of ethylene oxide in the tissues of B6C3F1 lacI transgenic mice following inhalation exposure","volume":"391","author":"Sisk","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0380","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.mrgentox.2012.01.003","article-title":"Ionizing radiation-induced mutant frequencies increase transiently in male germ cells of older mice","volume":"744","author":"Xu","year":"2012","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0385","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1002\/em.10108","article-title":"Heavy-ion-induced mutations in the gpt delta transgenic mouse: comparison of mutation spectra induced by heavy-ion, X-ray, and gamma-ray radiation","volume":"40","author":"Masumura","year":"2002","journal-title":"Environ. Mol. Mutagen"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0390","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1002\/(SICI)1098-2280(1999)34:2\/3<216::AID-EM22>3.0.CO;2-3","article-title":"Quantitative correlation between radiation-induced mutagenesis in endogenous genes and transgenes of mouse spermatogonial stem cells","volume":"34","author":"Martus","year":"1999","journal-title":"Environ. Mol. Mutagen"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0395","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S1383-5718(96)00118-0","article-title":"Detection of the two germ cell mutagens ENU and iPMS using the LacZ\/transgenic mouse mutation assay","volume":"388","author":"Liegibel","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0400","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1093\/toxsci\/kfh241","article-title":"No-Observed effect levels for carcinogenicity and for in vivo mutagenicity of a genotoxic carcinogen","volume":"81","author":"Hoshi","year":"2004","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0405","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S1383-5718(96)00120-9","article-title":"Germ cell mutagenesis in lacZ transgenic mice treated with methyl methanesulfonate","volume":"388","author":"Itoh","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0410","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S1383-5718(96)00119-2","article-title":"The detection of gene mutation in the tubular sperm of Muta Mice following a single intraperitoneal treatment with methyl methanesulphonate or ethylnitrosourea","volume":"388","author":"Brooks","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0415","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0027-5107(93)90215-2","article-title":"Transgenic systems for in vivo mutation analysis","volume":"288","author":"Provost","year":"1993","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0420","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S1383-5718(96)00113-1","article-title":"Germ-cell mutagenesis in lambda lacZ transgenic mice treated with ethylating and methylating agents: comparison with specific-locus test","volume":"388","author":"van Delft","year":"1997","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0425","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S1383-5718(99)00061-3","article-title":"Mutation induction by N-propyl-N-nitrosourea in eight MutaMouse organs","volume":"444","author":"Hara","year":"1999","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0430","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S1383-5718(00)00120-0","article-title":"Genotoxicity of o-aminoazotoluene (AAT) determined by the Ames test, the in vitro chromosomal aberration test, and the transgenic mouse gene mutation assay","volume":"471","author":"Ohsawa","year":"2000","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0435","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S0304-3835(99)00132-9","article-title":"Mutagenicity of 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP) in the new gpt delta transgenic mouse","volume":"143","author":"Masumura","year":"1999","journal-title":"Cancer Lett."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0440","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/S1383-5718(99)00060-1","article-title":"Procarbazine genotoxicity in the MutaMouse; strong clastogenicity and organ-specific induction of lacZ mutations","volume":"444","author":"Suzuki","year":"1999","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0445","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1002\/(SICI)1098-2280(1999)34:2\/3<190::AID-EM18>3.0.CO;2-#","article-title":"Evidence for the lack of base-change and small-deletion mutation induction by trichloroethylene in lacZ transgenic mice","volume":"34","author":"Douglas","year":"1999","journal-title":"Environ. Mol. Mutagen"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0450","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1002\/(SICI)1098-2280(1999)34:2\/3<195::AID-EM19>3.0.CO;2-I","article-title":"Spontaneous mutation frequency and pattern in Big Blue mice fed a vitamin E-supplemented diet","volume":"34","author":"Moore","year":"1999","journal-title":"Environ. Mol. Mutagen"},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0455","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1080\/09553000310001596931","article-title":"Molecular nature of mutations induced by irradiation with repeated low doses of X-rays in spleen, liver, brain and testis of lacZ-transgenic mice","volume":"79","author":"Ono","year":"2003","journal-title":"Int. J. Radiat. Biol."},{"key":"10.1016\/j.mrgentox.2018.05.021_bib0460","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.mrgentox.2016.09.009","article-title":"Dose-dependent de novo germline mutations detected by whole-exome sequencing in progeny of ENU-treated male gpt delta mice","volume":"810","author":"Masumura","year":"2016","journal-title":"Mutat. Res."}],"updated-by":[{"DOI":"10.1016\/j.mrgentox.2019.05.018","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T00:00:00Z","timestamp":1564617600000}}],"container-title":["Mutation Research\/Genetic Toxicology and Environmental Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1383571818301487?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1383571818301487?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T10:02:29Z","timestamp":1761300149000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1383571818301487"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8]]},"references-count":92,"alternative-id":["S1383571818301487"],"URL":"https:\/\/doi.org\/10.1016\/j.mrgentox.2018.05.021","relation":{},"ISSN":["1383-5718"],"issn-type":[{"value":"1383-5718","type":"print"}],"subject":[],"published":{"date-parts":[[2018,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identifying germ cell mutagens using OECD test guideline 488 (transgenic rodent somatic and germ cell gene mutation assays) and integration with somatic cell testing","name":"articletitle","label":"Article Title"},{"value":"Mutation Research\/Genetic Toxicology and Environmental Mutagenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mrgentox.2018.05.021","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Crown Copyright \u00a9 2018 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}