{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T21:20:57Z","timestamp":1781904057889,"version":"3.54.5"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"bilateral German-Croatian"},{"name":"Cost Action hCOMET"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Mutation Research\/Genetic Toxicology and Environmental Mutagenesis"],"published-print":{"date-parts":[[2019,7]]},"DOI":"10.1016\/j.mrgentox.2019.03.009","type":"journal-article","created":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T13:16:57Z","timestamp":1553779017000},"page":"3-10","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"special_numbering":"C","title":["Alkaline comet assay results on fresh and one-year frozen whole blood in small volume without cryo-protection in a group of people with different health status"],"prefix":"10.1016","volume":"843","author":[{"given":"Mirta","family":"Mili\u0107","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"O\u017evald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1382-5581","authenticated-orcid":false,"given":"Ivana","family":"Vinkovi\u0107 Vr\u010dek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7365-0627","authenticated-orcid":false,"given":"Marijana","family":"Vu\u010di\u0107 Lovren\u010di\u0107","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vi\u0161nja","family":"Ore\u0161\u010danin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3833-6717","authenticated-orcid":false,"given":"Stefano","family":"Bonassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilio Rojas","family":"Del Castillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mrgentox.2019.03.009_bib0005","doi-asserted-by":"crossref","DOI":"10.1093\/mutage\/geu061","article-title":"Worldwide interest in the comet assay: a bibliometric study","volume":"30","author":"Neri","year":"2015","journal-title":"Mutagenesis."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/mutage\/gex046","article-title":"The comet assay: ready for 30 more years","volume":"33","author":"Moller","year":"2018","journal-title":"Mutagenesis."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0015","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1385\/MB:26:3:249","article-title":"The comet assay for DNA damage and repair: principles, applications, and limitations","volume":"26","author":"Collins","year":"2004","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0020","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.mrgentox.2007.10.004","article-title":"Quantification of DNA repair capacity in whole blood of patients with head and neck cancer and healthy donors by comet assay","volume":"650","author":"Saha","year":"2008","journal-title":"Mutat. Res. Genet. Toxicol. Environ. Mutagen."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0025","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S1383-5718(03)00090-1","article-title":"Measurement of DNA breaks and oxidative damage in polymorphonuclear and mononuclear white blood cells: a novel approach using the comet assay","volume":"538","author":"Giovannelli","year":"2003","journal-title":"Mutat. Res. Genet. Toxicol. Environ. Mutagen."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0030","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1093\/mutage\/gew021","article-title":"The impact of lymphocyte isolation on induced DNA damage in human blood samples measured by the comet assay","volume":"31","author":"Bausinger","year":"2016","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0035","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.mrgentox.2006.03.002","article-title":"Reliable Comet assay measurements for detecting DNA damage induced by ionising radiation and chemicals","volume":"605","author":"Kumaravel","year":"2006","journal-title":"Mutat. Res. Genet. Toxicol. Environ. Mutagen."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0040","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1007\/s10517-008-0048-z","article-title":"Spontaneous DNA damage in peripheral blood leukocytes from donors of different age","volume":"145","author":"Sirota","year":"2008","journal-title":"Bull. Exp. Biol. Med."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0045","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0921-8777(00)00013-6","article-title":"Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subjects","volume":"460","author":"Hannon-Fletcher","year":"2000","journal-title":"Mutat. Res."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0050","series-title":"Chronic Professional Exposure to Low Doses of Ionizing Radiation","first-page":"437","article-title":"The Influence of Individual Genome Sensitivity in DNA Damage Repair Assessment","author":"Milic","year":"2011"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0055","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.mrgentox.2003.11.004","article-title":"Assessment of DNA damage by comet assay on frozen total blood: method and evaluation in smokers and non-smokers","volume":"558","author":"Hininger","year":"2004","journal-title":"Mutat. Res. Genet. Toxicol. Environ. Mutagen."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0060","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.dnarep.2018.01.005","article-title":"DNA damage in blood cells in relation to chemotherapy and nutritional status in colorectal cancer patients\u2014a pilot study","volume":"63","author":"Kv\u00e6rner","year":"2018","journal-title":"DNA Rep. (Amst)."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0065","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1002\/cbf.3016","article-title":"Leucocytes isolated from simply frozen whole blood can be used in human biomonitoring for DNA damage measurement with the comet assay","volume":"32","author":"Akor-Dewu","year":"2014","journal-title":"Cell Biochem. Funct."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0070","doi-asserted-by":"crossref","DOI":"10.1093\/mutage\/17.3.211","article-title":"Cryopreserved versus freshly isolated lymphocytes in human biomonitoring: endogenous and induced DNA damage, antioxidant status and repair capability","volume":"17","author":"Duthie","year":"2002","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0075","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/j.freeradbiomed.2011.05.020","article-title":"Simplified method for the collection, storage, and comet assay analysis of DNA damage in whole blood","volume":"51","author":"Al-Salmani","year":"2011","journal-title":"Free Radic. Biol. Med."},{"issue":"6","key":"10.1016\/j.mrgentox.2019.03.009_bib0080","first-page":"540","article-title":"Effect of storage on the suitability of whole blood samples to analysis by Comet assay","volume":"24","author":"Al-Salmani","year":"2009","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0085","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1093\/mutage\/gex034","article-title":"The comet assay in human biomonitoring: cryopreservation of whole blood and comparison with isolated mononuclear cells","volume":"33","author":"Koppen","year":"2018","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0090","doi-asserted-by":"crossref","DOI":"10.1096\/fasebj.24.1_supplement.535.2","article-title":"DNA damage determined by Single cell gel electrophoresis using cryopreserved lymphocyte, frozen whole blood in EDTA tube or DNA stabilized tube","volume":"24","author":"Ronchi","year":"2010","journal-title":"FASEB J."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0095","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1093\/mutage\/geu058","article-title":"Comparison of DNA damage by the comet assay in fresh versus cryopreserved peripheral blood mononuclear cells obtained following dietary intervention","volume":"30","author":"Del Bo\u2019","year":"2015","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0100","article-title":"The comet assay in human biomonitoring: cryopreservation of whole blood and comparison with isolated mononuclear cells","author":"Koppen","year":"2017","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0105","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1093\/carcin\/23.12.2129","article-title":"Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: an approach to consensus","volume":"23","author":"E. (European S.C. On O.D. Damage)","year":"2002","journal-title":"Carcinogenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0110","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1089\/bio.2011.0024","article-title":"Cryopreservation and storage effects on cell numbers and DNA damage in human lymphocytes","volume":"9","author":"Ho","year":"2011","journal-title":"Biopreserv. Biobank."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0115","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fgene.2014.00359","article-title":"Controlling variation in the comet assay","volume":"5","author":"Collins","year":"2014","journal-title":"Front. Genet."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0120","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0162082","article-title":"Oxidative stress, DNA Damage and DNA repair in female patients with diabetes mellitus type 2","volume":"11","author":"Grindel","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0125","first-page":"249","article-title":"Evaluation of in vitro genotoxic activity of bleomycin and mitomycin C in human lymphocytes using the alkaline comet assay","volume":"55","author":"Milic","year":"2004","journal-title":"Arh. Hig. Rada Toksikol."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0130","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1093\/mutage\/gex023","article-title":"Seasonal variations as predictive factors of the comet assay parameters: a retrospective study","volume":"33","author":"Geric","year":"2018","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0135","doi-asserted-by":"crossref","DOI":"10.1093\/mutage\/gep067","article-title":"Assessment and reduction of comet assay variation in relation to DNA damage: studies from the European Comet Assay Validation Group","author":"M\u00f8ller","year":"2010","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0140","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1093\/mutage\/gen015","article-title":"Statistical issues in the use of the comet assay","volume":"23","author":"Lovell","year":"2008","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0145","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.mrgentox.2014.09.006","article-title":"Critical issues with the in vivo comet assay: a report of the comet assay working group in the 6th International Workshop on Genotoxicity Testing (IWGT)","volume":"783","author":"Speit","year":"2015","journal-title":"Mutat. Res. Genet. Toxicol. Environ. Mutagen."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0150","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1002\/em.20694","article-title":"Comparison of Comet assay dose-response for ethyl methanesulfonate using freshly prepared versus cryopreserved tissues","volume":"53","author":"Recio","year":"2012","journal-title":"Environ. Mol. Mutagen."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0155","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1093\/mutage\/gep048","article-title":"Variation in the measurement of DNA damage by comet assay measured by the ECVAG y inter-laboratory validation trial","volume":"25","author":"Forchhammer","year":"2017","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0160","doi-asserted-by":"crossref","DOI":"10.1093\/mutage\/get001","article-title":"An ECVAG inter-laboratory validation study of the comet assay: inter-laboratory and intra-laboratory variations of DNA strand breaks and FPG-sensitive sites in human mononuclear cells","author":"Ersson","year":"2013","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0165","doi-asserted-by":"crossref","DOI":"10.1093\/mutage\/gep055","article-title":"An ECVAG trial on assessment of oxidative damage to DNA measured by the comet assay","author":"Johansson","year":"2010","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0170","doi-asserted-by":"crossref","DOI":"10.1016\/j.mrrev.2016.05.004","article-title":"Frequency of micronuclei and other biomarkers of DNA damage in populations exposed to dusts, asbestos and other fibers. A systematic review","volume":"770","author":"Bonassi","year":"2016","journal-title":"Mutat. Res. Rev. Mutat. Res."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0175","article-title":"How to best freeze liver samples to perform the in vivo mammalian alkaline comet assay","author":"Enciso Gadea","year":"2015","journal-title":"Front. Genet.Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0180","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1007\/s003940050006","article-title":"Recovery of human lymphocytes from oxidative DNA damage; the apparent enhancement of DNA repair by carotenoids is probably simply an antioxidant effect","volume":"39","author":"Torbergsen","year":"2000","journal-title":"Eur. J. Nutr."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0185","series-title":"DNA Comet Assay To Identify Different Freezing Temperatures of Irradiated Liver Chicken","author":"Duarte","year":"2009"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0190","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1093\/mutage\/gem018","article-title":"Suitability of cryopreserved isolated lymphocytes for the analysis of micronuclei with the cytokinesis-block method","volume":"22","author":"Zijno","year":"2007","journal-title":"Mutagenesis"},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0195","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1111\/j.1742-7843.2006.pto_167.x","article-title":"The alkaline comet assay: towards validation in biomonitoring of DNA damaging exposures","volume":"98","author":"Moller","year":"2006","journal-title":"Basic Clin. Pharmacol. Toxicol."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0200","first-page":"P33","article-title":"Impact of logistical variation in sample handling on comet results in human leukocytes","volume":"47","author":"Toraason","year":"2006","journal-title":"37th Annu. Meet. Abstr. Environ. Mutagen Soc."},{"key":"10.1016\/j.mrgentox.2019.03.009_bib0205","doi-asserted-by":"crossref","DOI":"10.1016\/j.mrfmmm.2014.11.009","article-title":"DNA damage in non-communicable diseases: a clinical and epidemiological perspective","volume":"776","author":"Milic","year":"2015","journal-title":"Mutat. Res. Fundam. Mol. Mech. Mutagen."}],"container-title":["Mutation Research\/Genetic Toxicology and Environmental Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1383571818303036?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1383571818303036?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T10:03:44Z","timestamp":1761300224000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1383571818303036"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7]]},"references-count":41,"alternative-id":["S1383571818303036"],"URL":"https:\/\/doi.org\/10.1016\/j.mrgentox.2019.03.009","relation":{},"ISSN":["1383-5718"],"issn-type":[{"value":"1383-5718","type":"print"}],"subject":[],"published":{"date-parts":[[2019,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Alkaline comet assay results on fresh and one-year frozen whole blood in small volume without cryo-protection in a group of people with different health status","name":"articletitle","label":"Article Title"},{"value":"Mutation Research\/Genetic Toxicology and Environmental Mutagenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mrgentox.2019.03.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}