{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T16:00:19Z","timestamp":1781193619805,"version":"3.54.1"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Fundacao para a Ciencia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BTM-SAL\/28882\/2017"],"award-info":[{"award-number":["PTDC\/BTM-SAL\/28882\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000021"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000021"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biomaterials Advances"],"published-print":{"date-parts":[[2022,3]]},"DOI":"10.1016\/j.msec.2021.112585","type":"journal-article","created":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T23:15:43Z","timestamp":1638486943000},"page":"112585","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":26,"special_numbering":"C","title":["Microfluidic-assisted electrospinning, an alternative to coaxial, as a controlled dual drug release system to treat inflammatory arthritic diseases"],"prefix":"10.1016","volume":"134","author":[{"given":"Filipa","family":"Vasconcelos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Bonani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catarina S.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Motta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Migliaresi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albino","family":"Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuno M.","family":"Neves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.msec.2021.112585_bb0005","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1016\/S0140-6736(16)30173-8","article-title":"Rheumatoid arthritis","volume":"388","author":"Smolen","year":"2016","journal-title":"Lancet"},{"key":"10.1016\/j.msec.2021.112585_bb0010","doi-asserted-by":"crossref","DOI":"10.1186\/ar4174","article-title":"Use of NSAIDs in treating patients with arthritis","volume":"15","author":"Crofford","year":"2013","journal-title":"Arthritis Res. Ther."},{"key":"10.1016\/j.msec.2021.112585_bb0015","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1136\/rmdopen-2017-000536","article-title":"Glucocorticoids in rheumatoid arthritis: current status and future studies","volume":"6","author":"Hua","year":"2020","journal-title":"RMD Open"},{"key":"10.1016\/j.msec.2021.112585_bb0020","article-title":"Rheumatoid arthritis: pathological mechanisms and modern pharmacologic therapies","volume":"6","author":"Guo","year":"2018","journal-title":"Bone Res. Rev."},{"key":"10.1016\/j.msec.2021.112585_bb0025","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s00296-016-3621-1","article-title":"Oral to subcutaneous methotrexate dose-conversion strategy in the treatment of rheumatoid arthritis","volume":"37","author":"Schiff","year":"2017","journal-title":"Rheumatol. Int."},{"key":"10.1016\/j.msec.2021.112585_bb0030","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.cyto.2017.04.012","article-title":"Immunomodulation of autoimmune arthritis by pro-inflammatory cytokines","volume":"98","author":"Kim","year":"2017","journal-title":"Cytokine"},{"key":"10.1016\/j.msec.2021.112585_bb0035","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1089\/ten.teb.2009.0536","article-title":"Impact of biological agents and tissue engineering approaches on the treatment of rheumatic diseases","volume":"16","author":"Da Silva","year":"2010","journal-title":"Tissue Eng. - Part B Rev."},{"key":"10.1016\/j.msec.2021.112585_bb0040","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1002\/smll.200801602","article-title":"Protein-based nanomedicine platforms for drug delivery","volume":"5","author":"MaHam","year":"2009","journal-title":"Small"},{"key":"10.1016\/j.msec.2021.112585_bb0045","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/s13770-018-00176-6","article-title":"Perspective on intra-articular injection cell therapy for osteoarthritis treatment","volume":"16","author":"Il Im","year":"2019","journal-title":"Tissue Eng.Regen. Med."},{"key":"10.1016\/j.msec.2021.112585_bb0050","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.ejpb.2009.06.009","article-title":"Intra-articular drug delivery systems for the treatment of rheumatic diseases: a review of the factors influencing their performance","volume":"73","author":"Butoescu","year":"2009","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"10.1016\/j.msec.2021.112585_bb0055","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/j.actbio.2008.11.006","article-title":"Preparation and characterization of starch-poly-\u03b5-caprolactone microparticles incorporating bioactive agents for drug delivery and tissue engineering applications","volume":"5","author":"Balmayor","year":"2009","journal-title":"Acta Biomater."},{"key":"10.1016\/j.msec.2021.112585_bb0060","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1023\/A:1015123024113","article-title":"Prolonged anti-inflammatory action of DL-lactide\/glycolide copolymer nanospheres containing betamethasone sodium phosphate for an intra-articular delivery system in antigen-induced arthritic rabbit","volume":"19","author":"Horisawa","year":"2002","journal-title":"Pharm. Res."},{"key":"10.1016\/j.msec.2021.112585_bb0065","doi-asserted-by":"crossref","first-page":"15172","DOI":"10.1039\/C4RA11438H","article-title":"Microfluidic spinning of fibrous alginate carrier having highly enhanced drug loading capability and delayed release profile","volume":"5","author":"Ahn","year":"2015","journal-title":"RSC Adv."},{"key":"10.1016\/j.msec.2021.112585_bb0070","doi-asserted-by":"crossref","first-page":"25712","DOI":"10.1039\/C9RA05012D","article-title":"Electrospun fibers and their application in drug controlled release, biological dressings, tissue repair, and enzyme immobilization","volume":"9","author":"Sun","year":"2019","journal-title":"RSC Adv."},{"key":"10.1016\/j.msec.2021.112585_bb0075","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1039\/b415094e","article-title":"Electrospinning of nanofibers with core-sheath, hollow, or porous structures","volume":"15","author":"McCann","year":"2005","journal-title":"J. Mater. Chem."},{"key":"10.1016\/j.msec.2021.112585_bb0080","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1021\/bm050743i","article-title":"Coaxial electrospinning of (fluorescein isothiocyanate-conjugated bovine serum albumin)-encapsulated poly(\u03b5-caprolactone) nanofibers for sustained release","volume":"7","author":"Zhang","year":"2006","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.msec.2021.112585_bb0085","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/967295","article-title":"Coaxial electrospinning with mixed solvents: from flat to round eudragit l100 nanofibers for better colon-targeted sustained drug release profiles","volume":"2014","author":"Yu","year":"2014","journal-title":"J. Nanomater."},{"key":"10.1016\/j.msec.2021.112585_bb0090","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.ijpharm.2018.12.026","article-title":"Controlled dual drug release by coaxial electrospun fibers \u2013 impact of the core fluid on drug encapsulation and release","volume":"556","author":"Wang","year":"2019","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.msec.2021.112585_bb0095","doi-asserted-by":"crossref","first-page":"6372","DOI":"10.1021\/acsnano.8b06032","article-title":"Controlled co-delivery of growth factors through layer-by-layer assembly of core shell nanofibers for improving bone regeneration","volume":"13","author":"Cheng","year":"2019","journal-title":"ACS Nano"},{"key":"10.1016\/j.msec.2021.112585_bb0100","doi-asserted-by":"crossref","DOI":"10.3390\/pharmaceutics11070305","article-title":"Drug delivery applications of core-sheath nanofibers prepared by coaxial electrospinning: a review","volume":"11","author":"Pant","year":"2019","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.msec.2021.112585_bb0105","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.biomaterials.2016.10.040","article-title":"Electrospinning versus microfluidic spinning of functional fibers for biomedical applications","volume":"114","author":"Cheng","year":"2017","journal-title":"Biomater. Rev."},{"key":"10.1016\/j.msec.2021.112585_bb0110","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.camwa.2012.01.062","article-title":"Modelling and simulation of processes in microfluidic devices for biomedical applications","volume":"64","author":"Cimr\u00e1k","year":"2012","journal-title":"Comput. Math. Appl."},{"key":"10.1016\/j.msec.2021.112585_bb0115","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1021\/acsabm.9b00853","article-title":"Micro fluidic methods for fabrication and engineering of nanoparticle drug delivery systems","volume":"3","author":"Zhang","year":"2020","journal-title":"ACS Appl. Bio Mater."},{"key":"10.1016\/j.msec.2021.112585_bb0120","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.addr.2011.11.013","article-title":"Microfabrication technologies for oral drug delivery","volume":"64","author":"Sant","year":"2012","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.msec.2021.112585_bb0125","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1088\/0957-4484\/7\/3\/009","article-title":"Nanometre diameter fibres of polymer, produced by electrospinning","volume":"7","author":"Reneker","year":"1999","journal-title":"Nanotechnology"},{"key":"10.1016\/j.msec.2021.112585_bb0130","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/nano9040567","article-title":"Biofunctional nanofibrous substrate for local TNF-capturing as a strategy to control inflammation in arthritic joints","volume":"9","author":"Bacelo","year":"2019","journal-title":"Nanomaterials."},{"key":"10.1016\/j.msec.2021.112585_bb0135","first-page":"2288","article-title":"A single dose, placebo controlled study of the fully human anti-tumor necrosis factor-\u03b1 antibody adalimumab (D2E7) in patients with rheumatoid arthritis","volume":"29","author":"Den Broeder","year":"2002","journal-title":"J. Rheumatol."},{"key":"10.1016\/j.msec.2021.112585_bb0140","first-page":"181","article-title":"Conditioned medium as a strategy for human stem cells chondrogenic differentiation","volume":"12","author":"Alves da Silva","year":"2013","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"10.1016\/j.msec.2021.112585_bb0145","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1136\/rmdopen-2016-000287","article-title":"Long-term study of the impact of methotrexate on serum cytokines and lymphocyte subsets in patients with active rheumatoid arthritis: correlation with pharmacokinetic measures","volume":"2","author":"Kremer","year":"2016","journal-title":"RMD Open"},{"key":"10.1016\/j.msec.2021.112585_bb0150","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1159\/000493390","article-title":"Rheumatoid arthritis: a brief overview of the treatment","volume":"27","author":"Bullock","year":"2019","journal-title":"Med. Princ. Pract."},{"key":"10.1016\/j.msec.2021.112585_bb0155","doi-asserted-by":"crossref","first-page":"3","DOI":"10.2217\/pgs-2019-0154","article-title":"Pharmacogenetics of methotrexate response in rheumatoid arthritis: an update","volume":"21","author":"Ling","year":"2019","journal-title":"Pharmacogenomics"},{"issue":"2015","key":"10.1016\/j.msec.2021.112585_bb0160","first-page":"279","article-title":"Update of the consensus statement of the Spanish Society of Rheumatology on the use of biological therapies in rheumatoid arthritis","volume":"11","author":"Sanmart\u00ed","year":"2014","journal-title":"Reumatol. Clin."},{"key":"10.1016\/j.msec.2021.112585_bb0165","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/art.39480","article-title":"American College of Rheumatology guideline for the treatment of rheumatoid arthritis","volume":"68","author":"Singh","year":"2016","journal-title":"Arthritis Rheumatol."},{"key":"10.1016\/j.msec.2021.112585_bb0170","first-page":"S95","article-title":"Side effects and management of side effects of methotrexate in rheumatoid arthritis","volume":"28","year":"2010","journal-title":"Clin. Exp. Rheumatol."},{"key":"10.1016\/j.msec.2021.112585_bb0175","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/j.progpolymsci.2010.04.002","article-title":"The return of a forgotten polymer - polycaprolactone in the 21st century","volume":"35","author":"Woodruff","year":"2010","journal-title":"Prog. Polym. Sci."},{"key":"10.1016\/j.msec.2021.112585_bb0180","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/ma13173853","article-title":"Electrospinning of PCL-based blends: processing optimization for their scalable production","volume":"13","author":"Arrieta","year":"2020","journal-title":"Materials (Basel)."},{"key":"10.1016\/j.msec.2021.112585_bb0185","doi-asserted-by":"crossref","first-page":"9265","DOI":"10.1016\/S0032-3861(00)00287-1","article-title":"Effect of crosslinking on the mechanical and thermal properties of poly(vinyl alcohol)","volume":"41","author":"Krumova","year":"2000","journal-title":"Polymer (Guildf)"},{"key":"10.1016\/j.msec.2021.112585_bb0190","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.actbio.2011.08.015","article-title":"Mechanical testing of electrospun PCL fibers","volume":"8","author":"Croisier","year":"2012","journal-title":"Acta Biomater."},{"key":"10.1016\/j.msec.2021.112585_bb0195","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.promfg.2015.07.098","article-title":"Influence of polyvinyl alcohol molecular weight on the electrospun nanofiber mechanical properties","volume":"2","author":"Ngadiman","year":"2015","journal-title":"Procedia Manuf."},{"key":"10.1016\/j.msec.2021.112585_bb0200","article-title":"The stiffness of living tissues and its implications for tissue engineering","author":"Guimar\u00e3es","year":"2020","journal-title":"Nat. Rev."},{"key":"10.1016\/j.msec.2021.112585_bb0205","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.jmbbm.2016.01.015","article-title":"Mechanical properties of normal and osteoarthritic human articular cartilage","volume":"61","author":"Robinson","year":"2016","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"10.1016\/j.msec.2021.112585_bb0210","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1093\/rheumatology\/kem279","article-title":"Recent insights in the pharmacological actions of methotrexate in the treatment of rheumatoid arthritis","volume":"47","author":"Wessels","year":"2008","journal-title":"Rheumatology"},{"key":"10.1016\/j.msec.2021.112585_bb0215","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1093\/rheumatology\/keg323","article-title":"Inhibition of cytokine production by methotrexate.Studies in healthy volunteers and patients with rheumatoid arthritis","volume":"42","author":"Gerards","year":"2003","journal-title":"Rheumatology"},{"key":"10.1016\/j.msec.2021.112585_bb0220","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/ijms19030768","article-title":"Structural biology of the TNF\u03b1 antagonists used in the treatment of rheumatoid arthritis","volume":"19","author":"Lim","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.msec.2021.112585_bb0225","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/327369","article-title":"Morphological characterization of nanofibers: methods and application in practice","volume":"2012","author":"\u0160irc","year":"2012","journal-title":"J. Nanomater."},{"key":"10.1016\/j.msec.2021.112585_bb0230","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1002\/smll.200801750","article-title":"Encapsulation of multiple biological compounds within a single electrospun fiber","volume":"5","author":"Dong","year":"2009","journal-title":"Small"},{"key":"10.1016\/j.msec.2021.112585_bb0235","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1002\/jbm.b.30510","article-title":"Modulation of protein release from biodegradable core \u2013 shell structured fibers prepared by coaxial electrospinning","author":"Jiang","year":"2006","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater.2"},{"key":"10.1016\/j.msec.2021.112585_bb0240","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.jconrel.2010.03.014","article-title":"Release pro file characteristics of biodegradable-polymer-coated drug particles fabricated by dual-capillary electrospray","volume":"145","author":"Lee","year":"2010","journal-title":"J. Control. Release"},{"key":"10.1016\/j.msec.2021.112585_bb0245","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.intimp.2014.08.002","article-title":"THP-1 cell line: an in vitro cell model for immune modulation approach","volume":"23","author":"Chanput","year":"2014","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.msec.2021.112585_bb0250","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1080\/21691401.2018.1460373","article-title":"Recent approaches for targeted drug delivery in rheumatoid arthritis diagnosis and treatment","volume":"46","author":"Pirmardvand Chegini","year":"2018","journal-title":"Artif. Cells Nanomed. Biotechnol."}],"container-title":["Biomaterials Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0928493121007256?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0928493121007256?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T21:07:46Z","timestamp":1760648866000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0928493121007256"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3]]},"references-count":50,"alternative-id":["S0928493121007256"],"URL":"https:\/\/doi.org\/10.1016\/j.msec.2021.112585","relation":{},"ISSN":["2772-9508"],"issn-type":[{"value":"2772-9508","type":"print"}],"subject":[],"published":{"date-parts":[[2022,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Microfluidic-assisted electrospinning, an alternative to coaxial, as a controlled dual drug release system to treat inflammatory arthritic diseases","name":"articletitle","label":"Article Title"},{"value":"Biomaterials Advances","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.msec.2021.112585","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"112585"}}