{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:12:09Z","timestamp":1792721529501,"version":"4.5.0"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81801012"],"award-info":[{"award-number":["81801012"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Materials Today Bio"],"published-print":{"date-parts":[[2022,6]]},"DOI":"10.1016\/j.mtbio.2022.100275","type":"journal-article","created":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T20:33:33Z","timestamp":1651523613000},"page":"100275","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Multi-omics analysis of oral bacterial biofilm on titanium oxide nanostructure modified implant surface: In vivo sequencing-based pilot study in beagle dogs"],"prefix":"10.1016","volume":"15","author":[{"given":"Hanyu","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruogu","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengchuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiucheng","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feilong","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9532-3598","authenticated-orcid":false,"given":"Xiaolin","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2000","key":"10.1016\/j.mtbio.2022.100275_bib1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/prd.12335","article-title":"Breaking the wave of peri-implantitis","volume":"84","author":"Fu","year":"2020","journal-title":"Periodontol"},{"issue":"Suppl 16","key":"10.1016\/j.mtbio.2022.100275_bib2","first-page":"S158","article-title":"Peri-implant health and disease. A systematic review of current epidemiology","volume":"42","author":"Derks","year":"2015","journal-title":"J.\u00a0Clin. Periodontol."},{"issue":"Suppl 11","key":"10.1016\/j.mtbio.2022.100275_bib3","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1111\/clr.12636","article-title":"Risk indicators for peri-implantitis. A narrative review","volume":"26","author":"Renvert","year":"2015","journal-title":"Clin. Oral Implants Res."},{"issue":"2000","key":"10.1016\/j.mtbio.2022.100275_bib4","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/prd.12280","article-title":"Biofilm as a risk factor in implant treatment","volume":"81","author":"Daubert","year":"2019","journal-title":"Periodontol"},{"issue":"Suppl 1","key":"10.1016\/j.mtbio.2022.100275_bib5","first-page":"S286","article-title":"Peri-implant diseases and conditions: consensus report of workgroup 4 of the 2017 World Workshop on the classification of periodontal and peri-implant diseases and conditions","volume":"89","author":"Berglundh","year":"2018","journal-title":"J.\u00a0Periodontol."},{"key":"10.1016\/j.mtbio.2022.100275_bib6","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/jbm.a.30722","article-title":"Significantly accelerated osteoblast cell growth on aligned TiO2 nanotubes","volume":"78","author":"Oh","year":"2006","journal-title":"J.\u00a0Biomed. Mater. Res."},{"key":"10.1016\/j.mtbio.2022.100275_bib7","first-page":"81","article-title":"Osteoblast behaviors on titania nanotube and mesopore layers","volume":"4","author":"Zhang","year":"2017","journal-title":"Regen Biomater"},{"key":"10.1016\/j.mtbio.2022.100275_bib8","doi-asserted-by":"crossref","first-page":"2130","DOI":"10.1073\/pnas.0813200106","article-title":"Stem cell fate dictated solely by altered nanotube dimension","volume":"106","author":"Oh","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mtbio.2022.100275_bib9","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1002\/jbm.a.32463","article-title":"Titanium dioxide nanotubes enhance bone bonding in vivo","volume":"92","author":"Bjursten","year":"2010","journal-title":"J.\u00a0Biomed. Mater. Res."},{"key":"10.1016\/j.mtbio.2022.100275_bib10","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.msec.2014.01.014","article-title":"Tantalum coating on TiO2 nanotubes induces superior rate of matrix mineralization and osteofunctionality in human osteoblasts","volume":"37","author":"Frandsen","year":"2014","journal-title":"Mater Sci Eng C Mater Biol Appl"},{"key":"10.1016\/j.mtbio.2022.100275_bib11","first-page":"3093","article-title":"Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1\/2 cell adhesion","volume":"8","author":"Peng","year":"2013","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.mtbio.2022.100275_bib12","doi-asserted-by":"crossref","first-page":"17080","DOI":"10.1021\/acsami.6b03152","article-title":"Antibacterial behavior of additively manufactured porous titanium with nanotubular surfaces releasing silver ions","volume":"8","author":"Amin Yavari","year":"2016","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.mtbio.2022.100275_bib13","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.2147\/IJN.S119750","article-title":"Decreased bacterial growth on titanium nanoscale topographies created by ion beam assisted evaporation","volume":"12","author":"Stolzoff","year":"2017","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.mtbio.2022.100275_bib14","doi-asserted-by":"crossref","first-page":"7217","DOI":"10.2147\/IJN.S216175","article-title":"Enhanced antibacterial properties of orthopedic implants by titanium nanotube surface modification: a review of current techniques","volume":"14","author":"Li","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.mtbio.2022.100275_bib15","doi-asserted-by":"crossref","DOI":"10.1002\/biot.202000116","article-title":"Surface engineering of biomaterials in orthopedic and dental implants: strategies to improve osteointegration, bacteriostatic and bactericidal activities","volume":"16","author":"Liu","year":"2021","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.mtbio.2022.100275_bib16","doi-asserted-by":"crossref","first-page":"5391","DOI":"10.3390\/ma14185391","article-title":"Electrophoretic deposition of biocompatible and bioactive hydroxyapatite-based coatings on titanium","volume":"14","author":"Djo\u0161i\u0107","year":"2021","journal-title":"Materials"},{"key":"10.1016\/j.mtbio.2022.100275_bib17","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.tibtech.2013.01.017","article-title":"Antibacterial surfaces: the quest for a new generation of biomaterials","volume":"31","author":"Hasan","year":"2013","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.mtbio.2022.100275_bib18","doi-asserted-by":"crossref","first-page":"17915","DOI":"10.1007\/s10853-021-06404-0","article-title":"Antimicrobial surfaces: a review of synthetic approaches, applicability and outlook","volume":"56","author":"Mahanta","year":"2021","journal-title":"J.\u00a0Mater. Sci."},{"key":"10.1016\/j.mtbio.2022.100275_bib19","doi-asserted-by":"crossref","first-page":"334","DOI":"10.3390\/ma10040334","article-title":"Calcium phosphate bioceramics: a review of their history, structure, properties, coating technologies and biomedical applications","volume":"10","author":"Eliaz","year":"2017","journal-title":"Materials"},{"key":"10.1016\/j.mtbio.2022.100275_bib20","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.actbio.2013.10.036","article-title":"Significance of calcium phosphate coatings for the enhancement of new bone osteogenesis--a review","volume":"10","author":"Surmenev","year":"2014","journal-title":"Acta Biomater."},{"key":"10.1016\/j.mtbio.2022.100275_bib21","first-page":"196","article-title":"Biofunctionalization of metallic implants by calcium phosphate coatings","volume":"4","author":"Su","year":"2019","journal-title":"Bioact. Mater."},{"key":"10.1016\/j.mtbio.2022.100275_bib22","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1002\/jbm.a.32862","article-title":"Antibacterial coatings of fluoridated hydroxyapatite for percutaneous implants","volume":"95","author":"Ge","year":"2010","journal-title":"J.\u00a0Biomed. Mater. Res."},{"key":"10.1016\/j.mtbio.2022.100275_bib23","article-title":"Implant-bone-interface: Reviewing the impact of titanium surface modifications on osteogenic processes in vitro and in vivo","volume":"7","author":"Stich","year":"2021","journal-title":"Bioeng Transl Med"},{"key":"10.1016\/j.mtbio.2022.100275_bib24","doi-asserted-by":"crossref","DOI":"10.1088\/1748-605X\/aac1a3","article-title":"Enhanced antibacterial efficacy of selective laser melting titanium surface with nanophase calcium phosphate embedded to TiO2 nanotubes","volume":"13","author":"Hu","year":"2018","journal-title":"Biomed. Mater."},{"key":"10.1016\/j.mtbio.2022.100275_bib25","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.msec.2018.12.045","article-title":"Functionalization of titania nanotubes with electrophoretically deposited silver and calcium phosphate nanoparticles: structure, composition and antibacterial assay","volume":"97","author":"Chernozem","year":"2019","journal-title":"Mater Sci Eng C Mater Biol Appl"},{"key":"10.1016\/j.mtbio.2022.100275_bib26","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1007\/s00203-018-1505-3","article-title":"Insights into the human oral microbiome","volume":"200","author":"Verma","year":"2018","journal-title":"Arch. Microbiol."},{"issue":"Suppl 1","key":"10.1016\/j.mtbio.2022.100275_bib27","first-page":"5","article-title":"Metagenomics, metatranscriptomics, and metabolomics approaches for microbiome analysis","volume":"12","author":"Aguiar-Pulido","year":"2016","journal-title":"Evol Bioinform Online"},{"issue":"2000","key":"10.1016\/j.mtbio.2022.100275_bib28","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1111\/j.1600-0757.1994.tb00008.x","article-title":"Clinical parameters for the evaluation of dental implants","volume":"4","author":"Mombelli","year":"1994","journal-title":"Periodontol"},{"key":"10.1016\/j.mtbio.2022.100275_bib29","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1111\/j.1600-051X.1986.tb00852.x","article-title":"Bleeding on probing. A predictor for the progression of periodontal disease?","volume":"13","author":"Lang","year":"1986","journal-title":"J.\u00a0Clin. Periodontol."},{"key":"10.1016\/j.mtbio.2022.100275_bib30","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1111\/j.1600-0765.1967.tb01887.x","article-title":"Errors in the clinical assessment of periodontal destruction","volume":"2","author":"Glavind","year":"1967","journal-title":"J.\u00a0Periodontal. Res."},{"key":"10.1016\/j.mtbio.2022.100275_bib31","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat. Methods"},{"key":"10.1016\/j.mtbio.2022.100275_bib32","doi-asserted-by":"crossref","first-page":"7537","DOI":"10.1128\/AEM.01541-09","article-title":"Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities","volume":"75","author":"Schloss","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mtbio.2022.100275_bib33","doi-asserted-by":"crossref","first-page":"1966","DOI":"10.1093\/bioinformatics\/btp336","article-title":"SOAP2: an improved ultrafast tool for short read alignment","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.mtbio.2022.100275_bib34","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-Seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mtbio.2022.100275_bib35","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1101\/gr.124321.111","article-title":"Differential expression in RNA-seq: a matter of depth","volume":"21","author":"Tarazona","year":"2011","journal-title":"Genome Res."},{"key":"10.1016\/j.mtbio.2022.100275_bib36","doi-asserted-by":"crossref","first-page":"R14","DOI":"10.1186\/gb-2010-11-2-r14","article-title":"Gene ontology analysis for RNA-seq: accounting for selection bias","volume":"11","author":"Young","year":"2010","journal-title":"Genome Biol."},{"key":"10.1016\/j.mtbio.2022.100275_bib37","doi-asserted-by":"crossref","first-page":"3787","DOI":"10.1093\/bioinformatics\/bti430","article-title":"Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary","volume":"21","author":"Mao","year":"2005","journal-title":"Bioinformatics"},{"key":"10.1016\/j.mtbio.2022.100275_bib38","series-title":"RStudio: Integrated Development Environment for R","author":"Team","year":"2015"},{"key":"10.1016\/j.mtbio.2022.100275_bib39","doi-asserted-by":"crossref","first-page":"105100","DOI":"10.1016\/j.micpath.2021.105100","article-title":"The temporal shift of peri-implant microbiota during the biofilm formation and maturation in a canine model","volume":"158","author":"Jiang","year":"2021","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.mtbio.2022.100275_bib40","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1186\/s12866-020-01982-6","article-title":"Oral microbial profile variation during canine ligature-induced peri-implantitis development","volume":"20","author":"Qiao","year":"2020","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.mtbio.2022.100275_bib41","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1111\/jcpe.12788","article-title":"Exploring the microbiome of healthy and diseased peri-implant sites using Illumina sequencing","volume":"44","author":"Sanz-Martin","year":"2017","journal-title":"J.\u00a0Clin. Periodontol."},{"key":"10.1016\/j.mtbio.2022.100275_bib42","doi-asserted-by":"crossref","first-page":"661","DOI":"10.3390\/microorganisms8050661","article-title":"The microbiome of peri-implantitis: a systematic review and meta-analysis","volume":"8","author":"Sahrmann","year":"2020","journal-title":"Microorganisms"},{"key":"10.1016\/j.mtbio.2022.100275_bib43","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1111\/jopr.13270","article-title":"Microbiological profile and human immune response associated with peri-implantitis: a systematic review","volume":"30","author":"Kensara","year":"2021","journal-title":"J.\u00a0Prosthodont."},{"key":"10.1016\/j.mtbio.2022.100275_bib44","doi-asserted-by":"crossref","first-page":"3157","DOI":"10.3390\/ijms19103157","article-title":"Influence of dental prosthesis and restorative materials interface on oral biofilms","volume":"19","author":"Hao","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.mtbio.2022.100275_bib45","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1111\/jre.12248","article-title":"Bacterial invasion and persistence: critical events in the pathogenesis of periodontitis?","volume":"50","author":"Ji","year":"2015","journal-title":"J.\u00a0Periodontal. Res."},{"key":"10.1016\/j.mtbio.2022.100275_bib46","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/jcpe.13046","article-title":"An appraisal of the role of specific bacteria in the initial pathogenesis of periodontitis","volume":"46","author":"Bartold","year":"2019","journal-title":"J.\u00a0Clin. Periodontol."},{"key":"10.1016\/j.mtbio.2022.100275_bib47","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.it.2017.08.005","article-title":"Tissue-specific immunity at the oral mucosal barrier","volume":"39","author":"Moutsopoulos","year":"2018","journal-title":"Trends Immunol."},{"issue":"2000","key":"10.1016\/j.mtbio.2022.100275_bib48","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1111\/prd.12150","article-title":"Comparison of peri-implant and periodontal marginal soft tissues in health and disease","volume":"76","author":"Ivanovski","year":"2018","journal-title":"Periodontol"},{"key":"10.1016\/j.mtbio.2022.100275_bib49","doi-asserted-by":"crossref","first-page":"5045","DOI":"10.2147\/IJN.S166661","article-title":"Micro-\/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human gingival fibroblasts and human gingival epithelial cells","volume":"13","author":"Xu","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.mtbio.2022.100275_bib50","doi-asserted-by":"crossref","first-page":"7185","DOI":"10.2147\/IJN.S239550","article-title":"Magnesium-doped nanostructured titanium surface modulates macrophage-mediated inflammatory response for ameliorative osseointegration","volume":"15","author":"Qiao","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.mtbio.2022.100275_bib51","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1002\/JPER.19-0634","article-title":"Peri-implant mucositis sites with suppuration have higher microbial risk than sites without suppuration","volume":"91","author":"Wang","year":"2020","journal-title":"J.\u00a0Periodontol."}],"container-title":["Materials Today Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2590006422000734?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2590006422000734?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:20:36Z","timestamp":1762906836000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2590006422000734"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6]]},"references-count":51,"alternative-id":["S2590006422000734"],"URL":"https:\/\/doi.org\/10.1016\/j.mtbio.2022.100275","relation":{},"ISSN":["2590-0064"],"issn-type":[{"value":"2590-0064","type":"print"}],"subject":[],"published":{"date-parts":[[2022,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Multi-omics analysis of oral bacterial biofilm on titanium oxide nanostructure modified implant surface: In vivo sequencing-based pilot study in beagle dogs","name":"articletitle","label":"Article Title"},{"value":"Materials Today Bio","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mtbio.2022.100275","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"100275"}}