{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:28:54Z","timestamp":1787027334527,"version":"build-2736575974"},"reference-count":69,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018806","name":"Department of Science and Technology of Hubei Province","doi-asserted-by":"publisher","award":["Q20241001"],"award-info":[{"award-number":["Q20241001"]}],"id":[{"id":"10.13039\/501100018806","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017589","name":"Hubei University","doi-asserted-by":"publisher","award":["NYWSWZX2025-2027-03"],"award-info":[{"award-number":["NYWSWZX2025-2027-03"]}],"id":[{"id":"10.13039\/501100017589","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017589","name":"Hubei University","doi-asserted-by":"publisher","award":["NYWSWZX2025-2027-10"],"award-info":[{"award-number":["NYWSWZX2025-2027-10"]}],"id":[{"id":"10.13039\/501100017589","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019937","name":"Hubei University State Key Laboratory of Biocatalysis and Enzyme Engineering","doi-asserted-by":"publisher","award":["SKLBEE2022007"],"award-info":[{"award-number":["SKLBEE2022007"]}],"id":[{"id":"10.13039\/501100019937","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010888","name":"Wuhan Municipal Science and Technology Innovation Bureau","doi-asserted-by":"publisher","award":["2025020602030105"],"award-info":[{"award-number":["2025020602030105"]}],"id":[{"id":"10.13039\/501100010888","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012554","name":"Hubei Provincial Department of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012554","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018526","name":"Key Research and Development Program of Wuhu","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018526","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Materials Today Bio"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.mtbio.2026.103336","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T06:21:48Z","timestamp":1780986108000},"page":"103336","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["De novo design of dual-target mini-binders simultaneously neutralizing Streptococcus equi subspecies zooepidemicus M-like protein and host TNFR1 confers complete protection against lethal infection"],"prefix":"10.1016","volume":"39","author":[{"given":"Ke","family":"Ming","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Banbin","family":"Xing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiting","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiujuan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"Geng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxia","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Weng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2445-6171","authenticated-orcid":false,"given":"Zigong","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"6515","key":"10.1016\/j.mtbio.2026.103336_bib1","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1126\/science.abd9909","article-title":"De novo design of picomolar SARS-CoV-2 miniprotein inhibitors","volume":"370","author":"Cao","year":"2020","journal-title":"Science"},{"issue":"16","key":"10.1016\/j.mtbio.2026.103336_bib2","doi-asserted-by":"crossref","first-page":"4305","DOI":"10.1016\/j.cell.2024.05.052","article-title":"Preclinical proof of principle for orally delivered Th17 antagonist miniproteins","volume":"187","author":"Berger","year":"2024","journal-title":"Cell"},{"issue":"7674","key":"10.1016\/j.mtbio.2026.103336_bib3","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1038\/nature23912","article-title":"Massively parallel de novo protein design for targeted therapeutics","volume":"550","author":"Chevalier","year":"2017","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.mtbio.2026.103336_bib4","doi-asserted-by":"crossref","first-page":"7064","DOI":"10.1038\/s41467-024-50919-4","article-title":"De novo design of miniprotein antagonists of cytokine storm inducers","volume":"15","author":"Huang","year":"2024","journal-title":"Nat. Commun."},{"issue":"6758","key":"10.1016\/j.mtbio.2026.103336_bib5","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1126\/science.adv0422","article-title":"De novo-designed pMHC binders facilitate T cell-mediated cytotoxicity toward cancer cells","volume":"389","author":"Johansen","year":"2025","journal-title":"Science"},{"issue":"Pt 2","key":"10.1016\/j.mtbio.2026.103336_bib6","article-title":"De novo design mini-binder proteins targeting the capsid-forming domain of type 2 PRRSV nucleocapsid protein to inhibit PRRSV replication in vitro","volume":"323","author":"Liu","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"issue":"1","key":"10.1016\/j.mtbio.2026.103336_bib7","doi-asserted-by":"crossref","first-page":"8521","DOI":"10.1038\/s41467-024-52582-1","article-title":"De novo design of mini-protein binders broadly neutralizing Clostridioides difficile toxin B variants","volume":"15","author":"Lv","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mtbio.2026.103336_bib8","article-title":"De Novo Design of Miniprotein Inhibitors of Bacterial Adhesins","author":"Chazin-Gray","year":"2025","journal-title":"bioRxiv"},{"issue":"52","key":"10.1016\/j.mtbio.2026.103336_bib9","doi-asserted-by":"crossref","DOI":"10.1002\/ange.202516058","article-title":"De Novo Design of High-Affinity Miniprotein Binders Targeting Francisella Tularensis Virulence Factor","volume":"137","author":"Gokce-Alpkilic","year":"2025","journal-title":"Angew. Chem."},{"issue":"7910","key":"10.1016\/j.mtbio.2026.103336_bib10","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1038\/s41586-022-04654-9","article-title":"Design of protein-binding proteins from the target structure alone","volume":"605","author":"Cao","year":"2022","journal-title":"Nature"},{"issue":"7976","key":"10.1016\/j.mtbio.2026.103336_bib11","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1038\/s41586-023-06415-8","article-title":"De novo design of protein structure and function with RFdiffusion","volume":"620","author":"Watson","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.mtbio.2026.103336_bib12","doi-asserted-by":"crossref","DOI":"10.4028\/b-XDTcg5","article-title":"Engineered dual-function antibody-like proteins to combat SARS-CoV-2-Induced immune dysregulation and inflammation","author":"Wang","year":"2025","journal-title":"Adv. Sci. (Weinh)"},{"issue":"8","key":"10.1016\/j.mtbio.2026.103336_bib13","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/s41573-019-0028-1","article-title":"Bispecific antibodies: a mechanistic review of the pipeline","volume":"18","author":"Labrijn","year":"2019","journal-title":"Nat. Rev. Drug Discov."},{"issue":"2","key":"10.1016\/j.mtbio.2026.103336_bib14","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.molimm.2015.01.003","article-title":"Alternative molecular formats and therapeutic applications for bispecific antibodies","volume":"67","author":"Spiess","year":"2015","journal-title":"Mol. Immunol."},{"issue":"24","key":"10.1016\/j.mtbio.2026.103336_bib15","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2219435120","article-title":"Membrane vesicle delivery of a streptococcal M protein disrupts the blood\u2013brain barrier by inducing autophagic endothelial cell death","volume":"120","author":"Pan","year":"2023","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"6","key":"10.1016\/j.mtbio.2026.103336_bib16","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.01742-22","article-title":"Protection efficacy of monoclonal antibodies targeting different regions of specific SzM protein from swine-isolated Streptococcus equi ssp. zooepidemicus strains","volume":"10","author":"Song","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.mtbio.2026.103336_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2025.140803","article-title":"Mini-binders targeting Streptococcus equi ssp. zooepidemicus M-like protein inhibit the bacterial adhesion and exert protective effects in vivo","volume":"304","author":"Ming","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.mtbio.2026.103336_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2025.142293","article-title":"De novo designed mini-binders targeting glyceraldehyde-3-phosphate dehydrogenase of Streptococcus equi ssp. zooepidemicus provided partial protection in mice model of infection","volume":"307","author":"Liu","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"issue":"7","key":"10.1016\/j.mtbio.2026.103336_bib19","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.3201\/eid1907.121365","article-title":"Transmission of Streptococcus equi subspecies zooepidemicus infection from horses to humans","volume":"19","author":"Pelkonen","year":"2013","journal-title":"Emerg. Infect. Dis."},{"issue":"1","key":"10.1016\/j.mtbio.2026.103336_bib20","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/1471-2334-6-36","article-title":"An outbreak of Streptococcus equi subspecies zooepidemicusassociated with consumption of fresh goat cheese","volume":"6","author":"Kuusi","year":"2006","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.mtbio.2026.103336_bib21","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.vetmic.2017.06.020","article-title":"Diversity of Streptococcus equi subsp. zooepidemicus strains isolated from the Spanish sheep and goat population and the identification, function and prevalence of a novel arbutin utilisation system","volume":"207","author":"Steward","year":"2017","journal-title":"Vet. Microbiol."},{"issue":"9","key":"10.1016\/j.mtbio.2026.103336_bib22","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1128\/jcm.34.9.2201-2204.1996","article-title":"Identification and molecular characterization of serological group C streptococci isolated from diseased pigs and monkeys in Indonesia","volume":"34","author":"Soedarmanto","year":"1996","journal-title":"J. Clin. Microbiol."},{"issue":"3","key":"10.1016\/j.mtbio.2026.103336_bib23","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000346","article-title":"Genomic evidence for the evolution of streptococcus equi: host restriction, increased virulence, and genetic exchange with human pathogens","volume":"5","author":"Holden","year":"2009","journal-title":"PLoS Pathog."},{"issue":"4","key":"10.1016\/j.mtbio.2026.103336_bib24","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1007\/s10096-006-0119-x","article-title":"Outbreak of Streptococcus equi subsp. zooepidemicus infections on the island of Gran Canaria associated with the consumption of inadequately pasteurized cheese","volume":"25","author":"Bordes-Ben\u00edtez","year":"2006","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mtbio.2026.103336_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.onehlt.2025.101063","article-title":"Occupational exposure and human carriage of Streptococcus equi subsp. zooepidemicus resulting in multiple livestock outbreaks","volume":"20","author":"Costa","year":"2025","journal-title":"One Health"},{"issue":"10","key":"10.1016\/j.mtbio.2026.103336_bib26","doi-asserted-by":"crossref","first-page":"2522","DOI":"10.3201\/eid2610.191485","article-title":"Streptococcus equi subspecies zooepidemicus and sudden deaths in Swine, Canada","volume":"26","author":"Costa","year":"2020","journal-title":"Emerg. Infect. Dis."},{"issue":"6","key":"10.1016\/j.mtbio.2026.103336_bib27","doi-asserted-by":"crossref","first-page":"2797","DOI":"10.1111\/tbed.13645","article-title":"Genetic characterization of Streptococcus equi subspecies zooepidemicus associated with high swine mortality in the United States","volume":"67","author":"Chen","year":"2020","journal-title":"Transbound. Emerg. Dis."},{"issue":"1","key":"10.1016\/j.mtbio.2026.103336_bib28","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1021\/acs.jproteome.6b00571","article-title":"Virulent and vaccine strains of Streptococcus equi ssp. zooepidemicus have different influences on phagocytosis and cytokine secretion of macrophages","volume":"16","author":"Jie","year":"2017","journal-title":"J. Proteome Res."},{"issue":"1","key":"10.1016\/j.mtbio.2026.103336_bib29","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.vetmic.2008.12.015","article-title":"Characterization of Streptococcus suis isolates from the diseased pigs in China between 2003 and 2007","volume":"137","author":"Wei","year":"2009","journal-title":"Vet. Microbiol."},{"issue":"6","key":"10.1016\/j.mtbio.2026.103336_bib30","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.tim.2010.02.007","article-title":"The streptococcal M protein: a highly versatile molecule","volume":"18","author":"Smeesters","year":"2010","journal-title":"Trends Microbiol."},{"issue":"3","key":"10.1016\/j.mtbio.2026.103336_bib31","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S0092-8674(04)00057-1","article-title":"A classical bacterial virulence determinant, forms complexes with fibrinogen that induce vascular leakage","volume":"116","author":"Herwald","year":"2004","journal-title":"Cell"},{"issue":"10","key":"10.1016\/j.mtbio.2026.103336_bib32","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1038\/s41564-017-0005-6","article-title":"Group A streptococcal M protein activates the NLRP3 inflammasome","volume":"2","author":"Valderrama","year":"2017","journal-title":"Nat. Microbiol."},{"issue":"2","key":"10.1016\/j.mtbio.2026.103336_bib33","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.4049\/jimmunol.177.2.1221","article-title":"Streptococcal M protein: a multipotent and powerful inducer of Inflammation1","volume":"177","author":"Pahlman","year":"2006","journal-title":"J. Immunol."},{"issue":"1","key":"10.1016\/j.mtbio.2026.103336_bib34","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00559-19","article-title":"Prominent binding of human and equine fibrinogen to Streptococcus equi subsp. zooepidemicus is mediated by specific SzM types and is a distinct phenotype of zoonotic isolates","volume":"88","author":"Bergmann","year":"2019","journal-title":"Infect. Immun."},{"issue":"2","key":"10.1016\/j.mtbio.2026.103336_bib35","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1128\/CMR.00101-13","article-title":"Disease manifestations and pathogenic mechanisms of group A streptococcus","volume":"27","author":"Walker Mark","year":"2014","journal-title":"Clin. Microbiol. Rev."},{"issue":"3","key":"10.1016\/j.mtbio.2026.103336_bib36","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1128\/CMR.00104-12","article-title":"Staphylococcal and streptococcal superantigen exotoxins","volume":"26","author":"Spaulding Adam","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"issue":"9","key":"10.1016\/j.mtbio.2026.103336_bib37","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1038\/nri1184","article-title":"Signalling pathways of the TNF superfamily: a double-edged sword","volume":"3","author":"Aggarwal","year":"2003","journal-title":"Nat. Rev. Immunol."},{"issue":"5","key":"10.1016\/j.mtbio.2026.103336_bib38","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1084\/jem.170.5.1627","article-title":"Antibodies to cachectin\/tumor necrosis factor reduce interleukin 1 beta and interleukin 6 appearance during lethal bacteremia","volume":"170","author":"Fong","year":"1989","journal-title":"J. Exp. Med."},{"issue":"1","key":"10.1016\/j.mtbio.2026.103336_bib39","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1093\/intimm\/dxu102","article-title":"Anti-TNF therapy: past, present and future","volume":"27","author":"Monaco","year":"2015","journal-title":"Int. Immunol."},{"key":"10.1016\/j.mtbio.2026.103336_bib40","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1146\/annurev.iy.07.040189.003205","article-title":"The biology of cachectin\/TNF--a primary mediator of the host response","volume":"7","author":"Beutler","year":"1989","journal-title":"Annu. Rev. Immunol."},{"issue":"9","key":"10.1016\/j.mtbio.2026.103336_bib41","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/S0962-8924(01)02064-5","article-title":"Signal transduction by tumor necrosis factor and its relatives","volume":"11","author":"Baud","year":"2001","journal-title":"Trends Cell. Biol."},{"issue":"1","key":"10.1016\/j.mtbio.2026.103336_bib42","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3390\/antib4010048","article-title":"Targeting sTNF\/TNFR1 signaling as a new therapeutic strategy","volume":"4","author":"Fischer","year":"2015","journal-title":"Antibodies"},{"issue":"2","key":"10.1016\/j.mtbio.2026.103336_bib43","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/j.cyto.2012.11.016","article-title":"Tumor Necrosis Factor gene polymorphism results in high TNF level in sepsis and septic shock","volume":"61","author":"Kothari","year":"2013","journal-title":"Cytokine"},{"issue":"8529","key":"10.1016\/j.mtbio.2026.103336_bib44","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/S0140-6736(87)91728-4","article-title":"Association between tumour necrosis factor in serum and fatal outcome in patients with meningococcal disease","volume":"329","author":"Waage","year":"1987","journal-title":"Lancet"},{"key":"10.1016\/j.mtbio.2026.103336_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2024.139403","article-title":"Design of minibinder proteins specific to TNFR1","volume":"293","author":"Weng","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"issue":"8","key":"10.1016\/j.mtbio.2026.103336_bib46","doi-asserted-by":"crossref","first-page":"3696","DOI":"10.1021\/acs.jctc.5b00255","article-title":"ff14SB: improving the accuracy of protein side chain and backbone parameters from ff99SB","volume":"11","author":"Maier","year":"2015","journal-title":"J. Chem. Theor. Comput."},{"issue":"20","key":"10.1016\/j.mtbio.2026.103336_bib47","doi-asserted-by":"crossref","first-page":"10096","DOI":"10.1063\/1.1808117","article-title":"A modified TIP3P water potential for simulation with Ewald summation","volume":"121","author":"Price","year":"2004","journal-title":"J. Chem. Phys."},{"issue":"10","key":"10.1016\/j.mtbio.2026.103336_bib48","doi-asserted-by":"crossref","first-page":"6281","DOI":"10.1021\/acs.jctc.1c00645","article-title":"gmx_MMPBSA: a new tool to perform end-state free energy calculations with GROMACS","volume":"17","author":"Vald\u00e9s-Tresanco","year":"2021","journal-title":"J. Chem. Theor. Comput."},{"issue":"9","key":"10.1016\/j.mtbio.2026.103336_bib49","doi-asserted-by":"crossref","first-page":"3314","DOI":"10.1021\/ct300418h","article-title":"MMPBSA.py: an efficient program for end-state free energy calculations","volume":"8","author":"Miller","year":"2012","journal-title":"J. Chem. Theor. Comput."},{"issue":"6615","key":"10.1016\/j.mtbio.2026.103336_bib50","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1126\/science.add2187","article-title":"Robust deep learning\u2013based protein sequence design using ProteinMPNN","volume":"378","author":"Dauparas","year":"2022","journal-title":"Science"},{"issue":"6","key":"10.1016\/j.mtbio.2026.103336_bib51","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1038\/s41592-022-01488-1","article-title":"ColabFold: making protein folding accessible to all","volume":"19","author":"Mirdita","year":"2022","journal-title":"Nat. Methods"},{"issue":"1","key":"10.1016\/j.mtbio.2026.103336_bib52","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1038\/s41467-023-38328-5","article-title":"Improving de novo protein binder design with deep learning","volume":"14","author":"Bennett","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mtbio.2026.103336_bib53","article-title":"HyperMPNN-A general strategy to design thermostable proteins learned from hyperthermophiles","author":"Ertelt","year":"2024","journal-title":"bioRxiv"},{"issue":"7","key":"10.1016\/j.mtbio.2026.103336_bib54","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1136\/annrheumdis-2020-217871","article-title":"Characteristics associated with hospitalisation for COVID-19 in people with rheumatic disease: data from the COVID-19 Global Rheumatology Alliance physician-reported registry","volume":"79","author":"Gianfrancesco","year":"2020","journal-title":"Ann. Rheum. Dis."},{"issue":"6","key":"10.1016\/j.mtbio.2026.103336_bib55","doi-asserted-by":"crossref","DOI":"10.1186\/cc13171","article-title":"Inhibition of the inflammatory cytokine tumor necrosis factor-alpha with etanercept provides protection against lethal H1N1 influenza infection in mice","volume":"17","author":"Shi","year":"2013","journal-title":"Crit. Care"},{"issue":"11","key":"10.1016\/j.mtbio.2026.103336_bib56","doi-asserted-by":"crossref","first-page":"4220","DOI":"10.4049\/jimmunol.136.11.4220","article-title":"Stimulation of neutrophils by tumor necrosis factor","volume":"136","author":"Klebanoff","year":"1986","journal-title":"J. Immunol."},{"issue":"9","key":"10.1016\/j.mtbio.2026.103336_bib57","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1016\/j.vaccine.2008.12.047","article-title":"Identification and characterization of a novel protective antigen, Enolase of Streptococcus suis serotype 2","volume":"27","author":"Zhang","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.mtbio.2026.103336_bib58","doi-asserted-by":"crossref","first-page":"465","DOI":"10.3389\/fcimb.2017.00465","article-title":"Streptococcus equi subsp. zooepidemicus invades and survives in epithelial cells","volume":"7","author":"Skive","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"16","key":"10.1016\/j.mtbio.2026.103336_bib59","doi-asserted-by":"crossref","first-page":"fnw164","DOI":"10.1093\/femsle\/fnw164","article-title":"Capsule of Streptococcus equi subsp. zooepidemicus hampers the adherence and invasion of epithelial and endothelial cells and is attenuated during internalization","volume":"363","author":"Xu","year":"2016","journal-title":"FEMS Microbiol. Lett."},{"issue":"2","key":"10.1016\/j.mtbio.2026.103336_bib60","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1128\/iai.62.2.442-448.1994","article-title":"M protein mediates streptococcal adhesion to HEp-2 cells","volume":"62","author":"Wang","year":"1994","journal-title":"Infect. Immun."},{"issue":"7","key":"10.1016\/j.mtbio.2026.103336_bib61","doi-asserted-by":"crossref","first-page":"2830","DOI":"10.1073\/pnas.88.7.2830","article-title":"Cloning and expression of cDNAs for two distinct murine tumor necrosis factor receptors demonstrate one receptor is species specific","volume":"88","author":"Lewis","year":"1991","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"1\u20132","key":"10.1016\/j.mtbio.2026.103336_bib62","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/0022-1759(84)90147-9","article-title":"Comparison of in vitro cell cytotoxic assays for tumor necrosis factor","volume":"68","author":"Flick","year":"1984","journal-title":"J. Immunol. Methods"},{"issue":"10","key":"10.1016\/j.mtbio.2026.103336_bib63","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1006\/cyto.1998.0492","article-title":"Modes of L929 cell death induced by TNF-alpha and other cytotoxic agents","volume":"11","author":"Humphreys","year":"1999","journal-title":"Cytokine"},{"issue":"1","key":"10.1016\/j.mtbio.2026.103336_bib64","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s12967-025-06122-0","article-title":"Exploring TNFR1: from discovery to targeted therapy development","volume":"23","author":"Li","year":"2025","journal-title":"J. Transl. Med."},{"issue":"5","key":"10.1016\/j.mtbio.2026.103336_bib65","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.4049\/jimmunol.141.5.1670","article-title":"Effect of cytokines on polymorphonuclear neutrophil infiltration in the mouse. Prostaglandin- and leukotriene-independent induction of infiltration by IL-1 and tumor necrosis factor","volume":"141","author":"Sayers","year":"1988","journal-title":"J. Immunol."},{"issue":"1","key":"10.1016\/j.mtbio.2026.103336_bib66","doi-asserted-by":"crossref","first-page":"5660","DOI":"10.1038\/s41467-023-41272-z","article-title":"De novo design of highly selective miniprotein inhibitors of integrins \u03b1v\u03b26 and \u03b1v\u03b28","volume":"14","author":"Roy","year":"2023","journal-title":"Nat. Commun."},{"issue":"4","key":"10.1016\/j.mtbio.2026.103336_bib67","doi-asserted-by":"crossref","first-page":"2399","DOI":"10.1007\/s10989-020-10033-7","article-title":"Prolonged benefit of reltecimod despite short plasma half-life","volume":"26","author":"Edgar","year":"2020","journal-title":"Int. J. Pept. Res. Therapeut."},{"issue":"19","key":"10.1016\/j.mtbio.2026.103336_bib68","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1001\/jama.295.19.2275","article-title":"Anti-TNF antibody therapy in rheumatoid arthritis and the risk of serious infections and malignancies - systematic review and meta-analysis of rare harmful effects in randomized controlled trials","volume":"295","author":"Bongartz","year":"2006","journal-title":"JAMA, J. Am. Med. Assoc."},{"issue":"4","key":"10.1016\/j.mtbio.2026.103336_bib69","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1016\/j.jaci.2016.06.033","article-title":"Interleukins (from IL-1 to IL-38), interferons, transforming growth factor \u03b2, and TNF-\u03b1: receptors, functions, and roles in diseases","volume":"138","author":"Akdis","year":"2016","journal-title":"J. Allergy Clin. Immunol."}],"container-title":["Materials Today Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2590006426005818?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2590006426005818?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:54:39Z","timestamp":1787025279000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2590006426005818"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":69,"alternative-id":["S2590006426005818"],"URL":"https:\/\/doi.org\/10.1016\/j.mtbio.2026.103336","relation":{},"ISSN":["2590-0064"],"issn-type":[{"value":"2590-0064","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"De novo design of dual-target mini-binders simultaneously neutralizing Streptococcus equi subspecies zooepidemicus M-like protein and host TNFR1 confers complete protection against lethal infection","name":"articletitle","label":"Article Title"},{"value":"Materials Today Bio","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mtbio.2026.103336","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"103336"}}