{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T21:58:15Z","timestamp":1792706295828,"version":"4.5.0"},"reference-count":82,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100011102","name":"European Union Seventh Framework Programme","doi-asserted-by":"publisher","award":["FP7\/2007-2013"],"award-info":[{"award-number":["FP7\/2007-2013"]}],"id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Neurobiology of Disease"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1016\/j.nbd.2017.09.004","type":"journal-article","created":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T03:45:42Z","timestamp":1504755942000},"page":"225-237","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":37,"special_numbering":"C","title":["mTOR-related neuropathology in mutant tsc2 zebrafish: Phenotypic, transcriptomic and pharmacological analysis"],"prefix":"10.1016","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6559-0572","authenticated-orcid":false,"given":"Chlo\u00eb","family":"Scheldeman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James D.","family":"Mills","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Siekierska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Serra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dani\u00eblle","family":"Copmans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anand M.","family":"Iyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin J.","family":"Whalley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Maes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna C.","family":"Jansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lieven","family":"Lagae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Aronica","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9989-9567","authenticated-orcid":false,"given":"Peter A.M.","family":"de Witte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.nbd.2017.09.004_bb0005","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1002\/ana.23943","article-title":"TORC1-dependent epilepsy caused by acute biallelic Tsc1 deletion in adult mice","volume":"74","author":"Abs","year":"2013","journal-title":"Ann. Neurol."},{"issue":"9","key":"10.1016\/j.nbd.2017.09.004_bb0010","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1038\/nrn3531","article-title":"Nuclear calcium signalling in the regulation of brain function","volume":"14","author":"Bading","year":"2013","journal-title":"Nat. Rev. Neurosci."},{"issue":"7422","key":"10.1016\/j.nbd.2017.09.004_bb0015","doi-asserted-by":"crossref","first-page":"114-8","DOI":"10.1038\/nature11537","article-title":"In vivo genome editing using a high-efficiency TALEN system","volume":"491","author":"Bedell","year":"2012","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.nbd.2017.09.004_bb0020","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/35036035","article-title":"The versatility and universality of calcium signalling","volume":"1","author":"Berridge","year":"2000","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"9869","key":"10.1016\/j.nbd.2017.09.004_bb0025","doi-asserted-by":"crossref","first-page":"817-24","DOI":"10.1016\/S0140-6736(12)61767-X","article-title":"Everolimus for angiomyolipoma associated with tuberous sclerosis complex or sporadic lymphangioleiomyomatosis (EXIST-2): a multicentre, randomised, double-blind, placebo-controlled trial","volume":"381","author":"Bissler","year":"2013","journal-title":"Lancet (London, England)."},{"key":"10.1016\/j.nbd.2017.09.004_bb0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.eplepsyres.2007.10.002","article-title":"Inflammatory processes in cortical tubers and subependymal giant cell tumors of tuberous sclerosis complex","volume":"78","author":"Boer","year":"2008","journal-title":"Epilepsy Res."},{"issue":"4","key":"10.1016\/j.nbd.2017.09.004_bb0035","doi-asserted-by":"crossref","first-page":"704-19","DOI":"10.1111\/j.1750-3639.2009.00341.x","article-title":"Gene expression analysis of tuberous sclerosis complex cortical tubers reveals increased expression of adhesion and inflammatory factors","volume":"20","author":"Boer","year":"2010","journal-title":"Brain pathology (Zurich, Switzerland)"},{"issue":"2","key":"10.1016\/j.nbd.2017.09.004_bb0040","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1003279","article-title":"Autophagy induction is a tor- and Tp53-independent cell survival response in a zebrafish model of disrupted ribosome biogenesis","volume":"9","author":"Boglev","year":"2013","journal-title":"PLoS Genet."},{"issue":"15","key":"10.1016\/j.nbd.2017.09.004_bb0045","doi-asserted-by":"crossref","first-page":"2114-20","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"issue":"6548","key":"10.1016\/j.nbd.2017.09.004_bb0050","doi-asserted-by":"crossref","first-page":"441-6","DOI":"10.1038\/377441a0","article-title":"Control of p70 s6 kinase by kinase activity of FRAP in vivo","volume":"377","author":"Brown","year":"1995","journal-title":"Nature"},{"issue":"11","key":"10.1016\/j.nbd.2017.09.004_bb0055","doi-asserted-by":"crossref","first-page":"740-4","DOI":"10.1136\/jmedgenet-2013-101680","article-title":"Disruption of TBC1D7, a subunit of the TSC1-TSC2 protein complex, in intellectual disability and megalencephaly","volume":"50","author":"Capo-Chichi","year":"2013","journal-title":"J. Med. Genet."},{"issue":"8","key":"10.1016\/j.nbd.2017.09.004_bb0060","doi-asserted-by":"crossref","first-page":"1227-33","DOI":"10.1002\/glia.22318","article-title":"Astrocyte calcium signaling and epilepsy","volume":"60","author":"Carmignoto","year":"2012","journal-title":"Glia"},{"issue":"12","key":"10.1016\/j.nbd.2017.09.004_bb0065","doi-asserted-by":"crossref","first-page":"1236-42","DOI":"10.1093\/jnen\/63.12.1236","article-title":"Pathogenesis of tuberous sclerosis subependymal giant cell astrocytomas: biallelic inactivation of TSC1 or TSC2 leads to mTOR activation","volume":"63","author":"Chan","year":"2004","journal-title":"J. Neuropathol. Exp. Neurol."},{"issue":"27","key":"10.1016\/j.nbd.2017.09.004_bb0070","doi-asserted-by":"crossref","first-page":"25485-90","DOI":"10.1074\/jbc.M501707200","article-title":"Phosphorylation of mammalian target of rapamycin (mTOR) at Ser-2448 is mediated by p70S6 kinase","volume":"280","author":"Chiang","year":"2005","journal-title":"J. Biol. Chem."},{"issue":"10","key":"10.1016\/j.nbd.2017.09.004_bb0075","doi-asserted-by":"crossref","first-page":"2366-82","DOI":"10.1038\/nprot.2007.324","article-title":"Integration of biological networks and gene expression data using cytoscape","volume":"2","author":"Cline","year":"2007","journal-title":"Nat. Protoc."},{"issue":"5","key":"10.1016\/j.nbd.2017.09.004_bb0080","doi-asserted-by":"crossref","first-page":"427-36","DOI":"10.1177\/1087057115618348","article-title":"A KNIME-based analysis of the zebrafish Photomotor response clusters the phenotypes of 14 classes of neuroactive molecules","volume":"21","author":"Copmans","year":"2016","journal-title":"J. Biomol. Screen."},{"issue":"12","key":"10.1016\/j.nbd.2017.09.004_bb0085","doi-asserted-by":"crossref","first-page":"734-42","DOI":"10.1016\/j.molmed.2011.07.008","article-title":"mTOR: a pathogenic signaling pathway in developmental brain malformations","volume":"17","author":"Crino","year":"2011","journal-title":"Trends Mol. Med."},{"issue":"13","key":"10.1016\/j.nbd.2017.09.004_bb0090","doi-asserted-by":"crossref","first-page":"1345-56","DOI":"10.1056\/NEJMra055323","article-title":"The tuberous sclerosis complex","volume":"355","author":"Crino","year":"2006","journal-title":"N. Engl. J. Med."},{"issue":"21","key":"10.1016\/j.nbd.2017.09.004_bb0095","doi-asserted-by":"crossref","first-page":"1716-23","DOI":"10.1212\/WNL.0b013e3181e04325","article-title":"Biallelic TSC gene inactivation in tuberous sclerosis complex","volume":"74","author":"Crino","year":"2010","journal-title":"Neurology"},{"issue":"7","key":"10.1016\/j.nbd.2017.09.004_bb0100","doi-asserted-by":"crossref","first-page":"733-45","DOI":"10.1016\/S1474-4422(15)00069-1","article-title":"Neurological and neuropsychiatric aspects of tuberous sclerosis complex","volume":"14","author":"Curatolo","year":"2015","journal-title":"The Lancet Neurology."},{"issue":"10","key":"10.1016\/j.nbd.2017.09.004_bb0105","doi-asserted-by":"crossref","first-page":"1771-80","DOI":"10.1007\/s00467-014-3027-9","article-title":"Tuberous sclerosis complex: the past and the future","volume":"30","author":"De Waele","year":"2015","journal-title":"Pediatr. Nephrol."},{"issue":"16","key":"10.1016\/j.nbd.2017.09.004_bb0110","doi-asserted-by":"crossref","first-page":"3439-40","DOI":"10.1093\/bioinformatics\/bti525","article-title":"BioMart and Bioconductor: a powerful link between biological databases and microarray data analysis","volume":"21","author":"Durinck","year":"2005","journal-title":"Bioinformatics"},{"key":"10.1016\/j.nbd.2017.09.004_bb0115","doi-asserted-by":"crossref","DOI":"10.1038\/sj.onc.1207542","article-title":"Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression","volume":"23","author":"Fingar","year":"2004","journal-title":"Oncogene"},{"key":"10.1016\/j.nbd.2017.09.004_bb0120","doi-asserted-by":"crossref","DOI":"10.1016\/S0140-6736(12)61134-9","article-title":"Efficacy and safety of everolimus for subependymal giant cell astrocytomas associated with tuberous sclerosis complex (EXIST-1): a multicentre, randomised, placebo-controlled phase 3 trial","volume":"381","author":"Franz","year":"2013","journal-title":"Lancet"},{"key":"10.1016\/j.nbd.2017.09.004_bb0125","doi-asserted-by":"crossref","DOI":"10.1016\/S0140-6736(16)31419-2","article-title":"Adjunctive everolimus therapy for treatment-resistant focal-onset seizures associated with tuberous sclerosis (EXIST-3): a phase 3, randomised, double-blind, placebo-controlled study","author":"French","year":"2016","journal-title":"Lancet (London, England)."},{"issue":"43","key":"10.1016\/j.nbd.2017.09.004_bb0130","doi-asserted-by":"crossref","first-page":"11083-110","DOI":"10.1523\/JNEUROSCI.2766-06.2006","article-title":"Potential new antiepileptogenic targets indicated by microarray analysis in a rat model for temporal lobe epilepsy","volume":"26","author":"Gorter","year":"2006","journal-title":"J. Neurosci."},{"issue":"10","key":"10.1016\/j.nbd.2017.09.004_bb0135","first-page":"1935-8","article-title":"Hemimegalencephaly and focal megalencephaly in tuberous sclerosis complex","volume":"19","author":"Griffiths","year":"1998","journal-title":"Am. J. Neuroradiol."},{"issue":"7","key":"10.1016\/j.nbd.2017.09.004_bb0140","doi-asserted-by":"crossref","first-page":"715-26","DOI":"10.1517\/17460440902988464","article-title":"Using zebrafish to assess the impact of drugs on neural development and function","volume":"4","author":"Guo","year":"2009","journal-title":"Expert Opin. Drug Discovery"},{"issue":"6","key":"10.1016\/j.nbd.2017.09.004_bb0145","first-page":"1639-47","article-title":"Loss of tuberin in both subependymal giant cell astrocytomas and angiomyolipomas supports a two-hit model for the pathogenesis of tuberous sclerosis tumors","volume":"151","author":"Henske","year":"1997","journal-title":"Am. J. Pathol."},{"issue":"28","key":"10.1016\/j.nbd.2017.09.004_bb0150","doi-asserted-by":"crossref","first-page":"26089-93","DOI":"10.1074\/jbc.M504045200","article-title":"Identification of S6 kinase 1 as a novel mammalian target of rapamycin (mTOR)-phosphorylating kinase","volume":"280","author":"Holz","year":"2005","journal-title":"J. Biol. Chem."},{"issue":"3\u20134","key":"10.1016\/j.nbd.2017.09.004_bb0155","first-page":"144-8","article-title":"Zebrafish as a model for studying genetic aspects of epilepsy","volume":"3","author":"Hortopan","year":"2010","journal-title":"Dis. Model. Mech."},{"issue":"7","key":"10.1016\/j.nbd.2017.09.004_bb0160","doi-asserted-by":"crossref","first-page":"1981-91","DOI":"10.1091\/mbc.e08-12-1248","article-title":"Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy","volume":"20","author":"Hosokawa","year":"2009","journal-title":"Mol. Biol. Cell"},{"issue":"7446","key":"10.1016\/j.nbd.2017.09.004_bb0165","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nature12111","article-title":"The zebrafish reference genome sequence and its relationship to the human genome","volume":"496","author":"Howe","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.nbd.2017.09.004_bb0170","doi-asserted-by":"crossref","first-page":"229-41","DOI":"10.1111\/j.0105-2896.2005.00310.x","article-title":"The lysosomal cysteine proteases in MHC class II antigen presentation","volume":"207","author":"Hsing","year":"2005","journal-title":"Immunol. Rev."},{"issue":"1","key":"10.1016\/j.nbd.2017.09.004_bb0175","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources","volume":"4","author":"Huang da","year":"2009","journal-title":"Nat. Protoc."},{"issue":"2","key":"10.1016\/j.nbd.2017.09.004_bb0180","doi-asserted-by":"crossref","first-page":"179-90","DOI":"10.1042\/BJ20080281","article-title":"The TSC1\u2013TSC2 complex: a molecular switchboard controlling cell growth","volume":"412","author":"Huang","year":"2008","journal-title":"The Biochemical journal."},{"key":"10.1016\/j.nbd.2017.09.004_bb0185","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s12989-016-0129-5","article-title":"Extracellular cathepsin S and intracellular caspase 1 activation are surrogate biomarkers of particulate-induced lysosomal disruption in macrophages","volume":"13","author":"Hughes","year":"2016","journal-title":"Part Fibre Toxicol."},{"issue":"Pt 6","key":"10.1016\/j.nbd.2017.09.004_bb0190","first-page":"1613-28","article-title":"PI3K\/AKT pathway mutations cause a spectrum of brain malformations from megalencephaly to focal cortical dysplasia","volume":"138","author":"Jansen","year":"2015","journal-title":"Brain"},{"issue":"11","key":"10.1016\/j.nbd.2017.09.004_bb0195","doi-asserted-by":"crossref","first-page":"1017-27","DOI":"10.1515\/BC.2004.132","article-title":"Cysteine cathepsins in human cancer","volume":"385","author":"Jedeszko","year":"2004","journal-title":"Biol. Chem."},{"issue":"5","key":"10.1016\/j.nbd.2017.09.004_bb0200","doi-asserted-by":"crossref","first-page":"389-94","DOI":"10.1007\/s10916-006-9023-2","article-title":"Support vector machine-based feature selection for classification of liver fibrosis grade in chronic hepatitis C","volume":"30","author":"Jiang","year":"2006","journal-title":"J. Med. Syst."},{"issue":"2","key":"10.1016\/j.nbd.2017.09.004_bb0205","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.tips.2013.12.002","article-title":"Zebrafish as an emerging model for studying complex brain disorders","volume":"35","author":"Kalueff","year":"2014","journal-title":"Trends Pharmacol. Sci."},{"issue":"2","key":"10.1016\/j.nbd.2017.09.004_bb0210","doi-asserted-by":"crossref","first-page":"255-67","DOI":"10.1242\/dmm.005587","article-title":"Zebrafish model of tuberous sclerosis complex reveals cell-autonomous and non-cell-autonomous functions of mutant tuberin","volume":"4","author":"Kim","year":"2011","journal-title":"Dis. Model. Mech."},{"issue":"4","key":"10.1016\/j.nbd.2017.09.004_bb0215","doi-asserted-by":"crossref","first-page":"R36","DOI":"10.1186\/gb-2013-14-4-r36","article-title":"TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions","volume":"14","author":"Kim","year":"2013","journal-title":"Genome Biol."},{"issue":"1","key":"10.1016\/j.nbd.2017.09.004_bb0220","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/15548627.2015.1100356","article-title":"Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)","volume":"12","author":"Klionsky","year":"2016","journal-title":"Autophagy"},{"issue":"3","key":"10.1016\/j.nbd.2017.09.004_bb0225","doi-asserted-by":"crossref","first-page":"121-6","DOI":"10.2165\/11634580-000000000-00000","article-title":"Topical rapamycin therapy to alleviate the cutaneous manifestations of tuberous sclerosis complex: a double-blind, randomized, controlled trial to evaluate the safety and efficacy of topically applied rapamycin","volume":"12","author":"Koenig","year":"2012","journal-title":"Drugs in R&D."},{"issue":"3","key":"10.1016\/j.nbd.2017.09.004_bb0230","doi-asserted-by":"crossref","first-page":"231-7","DOI":"10.1038\/nchembio.307","article-title":"Rapid behavior\u2014based identification of neuroactive small molecules in the zebrafish","volume":"6","author":"Kokel","year":"2010","journal-title":"Nat. Chem. Biol."},{"issue":"9","key":"10.1016\/j.nbd.2017.09.004_bb0235","doi-asserted-by":"crossref","first-page":"3834-43","DOI":"10.1523\/JNEUROSCI.3689-12.2013","article-title":"Identification of nonvisual photomotor response cells in the vertebrate hindbrain","volume":"33","author":"Kokel","year":"2013","journal-title":"J. Neurosci."},{"key":"10.1016\/j.nbd.2017.09.004_bb0240","doi-asserted-by":"crossref","first-page":"W345-9","DOI":"10.1093\/nar\/gkm391","article-title":"CPC: assess the protein-coding potential of transcripts using sequence features and support vector machine","volume":"35","author":"Kong","year":"2007","journal-title":"Nucleic Acids Res."},{"issue":"8","key":"10.1016\/j.nbd.2017.09.004_bb0245","doi-asserted-by":"crossref","first-page":"941-5","DOI":"10.1038\/ng.2329","article-title":"De novo somatic mutations in components of the PI3K-AKT3-mTOR pathway cause hemimegalencephaly","volume":"44","author":"Lee","year":"2012","journal-title":"Nat. Genet."},{"issue":"4","key":"10.1016\/j.nbd.2017.09.004_bb0250","first-page":"848-60","article-title":"The lysosomal cysteine protease, cathepsin S, is increased in Alzheimer's disease and down syndrome brain. An immunocytochemical study","volume":"146","author":"Lemere","year":"1995","journal-title":"Am. J. Pathol."},{"issue":"1","key":"10.1016\/j.nbd.2017.09.004_bb0255","doi-asserted-by":"crossref","first-page":"148-59","DOI":"10.1152\/jn.00839.2011","article-title":"Touch responsiveness in zebrafish requires voltage-gated calcium channel 2.1b","volume":"108","author":"Low","year":"2012","journal-title":"J. Neurophysiol."},{"key":"10.1016\/j.nbd.2017.09.004_bb0260","doi-asserted-by":"crossref","first-page":"4563","DOI":"10.1038\/ncomms5563","article-title":"Selective suppression of excessive GluN2C expression rescues early epilepsy in a tuberous sclerosis murine model","volume":"5","author":"Lozovaya","year":"2014","journal-title":"Nat. Commun."},{"issue":"5","key":"10.1016\/j.nbd.2017.09.004_bb0265","doi-asserted-by":"crossref","first-page":"307-18","DOI":"10.1038\/nrm2672","article-title":"Molecular mechanisms of mTOR-mediated translational control","volume":"10","author":"Ma","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"4","key":"10.1016\/j.nbd.2017.09.004_bb0270","doi-asserted-by":"crossref","first-page":"443-6","DOI":"10.1177\/088307389400900424","article-title":"Unilateral megalencephaly and tuberous sclerosis: related disorders?","volume":"9","author":"Maloof","year":"1994","journal-title":"J. Child Neurol."},{"issue":"5","key":"10.1016\/j.nbd.2017.09.004_bb0275","doi-asserted-by":"crossref","first-page":"685-93","DOI":"10.1007\/s00401-012-0950-3","article-title":"Cytoarchitectural alterations are widespread in cerebral cortex in tuberous sclerosis complex","volume":"123","author":"Marcotte","year":"2012","journal-title":"Acta Neuropathol."},{"issue":"2","key":"10.1016\/j.nbd.2017.09.004_bb0280","doi-asserted-by":"crossref","first-page":"129-33","DOI":"10.2350\/06-04-0081.1","article-title":"Sudden unexpected death in childhood associated with cardiac rhabdomyoma, involuting adrenal ganglioneuroma, and megalencephaly: another expression of tuberous sclerosis?","volume":"10","author":"Masoumi","year":"2007","journal-title":"Pediatric and developmental pathology: the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society."},{"issue":"2","key":"10.1016\/j.nbd.2017.09.004_bb0285","doi-asserted-by":"crossref","first-page":"216-20","DOI":"10.1038\/79951","article-title":"Effective targeted gene \u2018knockdown\u2019 in zebrafish","volume":"26","author":"Nasevicius","year":"2000","journal-title":"Nat. Genet."},{"issue":"1","key":"10.1016\/j.nbd.2017.09.004_bb0290","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/cga.12079","article-title":"Zebrafish as a systems toxicology model for developmental neurotoxicity testing","volume":"55","author":"Nishimura","year":"2015","journal-title":"Congenital Anomalies."},{"issue":"10","key":"10.1016\/j.nbd.2017.09.004_bb0295","doi-asserted-by":"crossref","first-page":"1602-10","DOI":"10.1111\/epi.12761","article-title":"Interictal high frequency oscillations correlating with seizure outcome in patients with widespread epileptic networks in tuberous sclerosis complex","volume":"55","author":"Okanishi","year":"2014","journal-title":"Epilepsia"},{"key":"10.1016\/j.nbd.2017.09.004_bb0300","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0091-679X(04)77003-X","article-title":"Behavioral screening assays in zebrafish","volume":"77","author":"Orger","year":"2004","journal-title":"Methods Cell Biol."},{"issue":"1","key":"10.1016\/j.nbd.2017.09.004_bb0305","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/j.1750-3639.2012.00616.x","article-title":"Fetal brain lesions in tuberous sclerosis complex: TORC1 activation and inflammation","volume":"23","author":"Prabowo","year":"2013","journal-title":"Brain pathology (Zurich, Switzerland)"},{"issue":"6","key":"10.1016\/j.nbd.2017.09.004_bb0310","doi-asserted-by":"crossref","first-page":"1096-105","DOI":"10.1111\/j.1750-3639.2010.00416.x","article-title":"Analysis of TSC cortical tubers by deep sequencing of TSC1, TSC2 and KRAS demonstrates that small second-hit mutations in these genes are rare events","volume":"20","author":"Qin","year":"2010","journal-title":"Brain Pathol."},{"issue":"16","key":"10.1016\/j.nbd.2017.09.004_bb0315","doi-asserted-by":"crossref","first-page":"8591-601","DOI":"10.1074\/jbc.M115.701870","article-title":"Structural basis of the interaction between tuberous sclerosis complex 1 (TSC1) and Tre2-Bub2-Cdc16 domain family member 7 (TBC1D7)","volume":"291","author":"Qin","year":"2016","journal-title":"J. Biol. Chem."},{"issue":"1","key":"10.1016\/j.nbd.2017.09.004_bb0320","doi-asserted-by":"crossref","first-page":"62-6","DOI":"10.1016\/S0304-3940(02)01423-4","article-title":"Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data","volume":"339","author":"Ramakers","year":"2003","journal-title":"Neurosci. Lett."},{"issue":"6","key":"10.1016\/j.nbd.2017.09.004_bb0325","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkp045","article-title":"Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data","volume":"37","author":"Ruijter","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.nbd.2017.09.004_bb0330","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/B978-0-444-53613-6.00004-6","article-title":"Development of motor rhythms in zebrafish embryos","volume":"187","author":"Saint-Amant","year":"2010","journal-title":"Prog. Brain Res."},{"key":"10.1016\/j.nbd.2017.09.004_bb0335","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/1471-213X-11-71","article-title":"Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a\u2212\/\u2212 mutants","volume":"11","author":"Sapetto-Rebow","year":"2011","journal-title":"BMC Dev. Biol."},{"issue":"3","key":"10.1016\/j.nbd.2017.09.004_bb0340","doi-asserted-by":"crossref","first-page":"155-62","DOI":"10.1038\/nrm3757","article-title":"Making new contacts: the mTOR network in metabolism and signalling crosstalk","volume":"15","author":"Shimobayashi","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"2","key":"10.1016\/j.nbd.2017.09.004_bb0345","doi-asserted-by":"crossref","first-page":"385-93","DOI":"10.1007\/s00441-014-1849-1","article-title":"Calcium signaling and epilepsy","volume":"357","author":"Steinlein","year":"2014","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.nbd.2017.09.004_bb0350","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.semcdb.2016.01.040","article-title":"The role of mTOR signalling in neurogenesis, insights from tuberous sclerosis complex","volume":"52","author":"Tee","year":"2016","journal-title":"Semin. Cell Dev. Biol."},{"issue":"3","key":"10.1016\/j.nbd.2017.09.004_bb0355","doi-asserted-by":"crossref","first-page":"562-78","DOI":"10.1038\/nprot.2012.016","article-title":"Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks","volume":"7","author":"Trapnell","year":"2012","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.nbd.2017.09.004_bb0360","doi-asserted-by":"crossref","first-page":"172-80","DOI":"10.1016\/j.ymeth.2014.12.004","article-title":"Autophagy in zebrafish","volume":"75","author":"Varga","year":"2015","journal-title":"Methods"},{"issue":"11","key":"10.1016\/j.nbd.2017.09.004_bb0365","doi-asserted-by":"crossref","first-page":"1724-43","DOI":"10.1111\/j.1528-1167.2005.00298.x","article-title":"Brain inflammation in epilepsy: experimental and clinical evidence","volume":"46","author":"Vezzani","year":"2005","journal-title":"Epilepsia"},{"issue":"D1","key":"10.1016\/j.nbd.2017.09.004_bb0370","doi-asserted-by":"crossref","first-page":"D1036-44","DOI":"10.1093\/nar\/gkv1165","article-title":"DGIdb 2.0: Mining clinically relevant drug-gene interactions","volume":"44","author":"Wagner","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.nbd.2017.09.004_bb0375","doi-asserted-by":"crossref","DOI":"10.1093\/hmg\/ddp025","article-title":"Loss of Tsc2 in radial glia models the brain pathology of tuberous sclerosis complex in the mouse","volume":"18","author":"Way","year":"2009","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.nbd.2017.09.004_bb0380","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.brainres.2008.07.067","article-title":"Upregulation of cathepsin S in the aging and pathological nervous system of mice","volume":"1232","author":"Wendt","year":"2008","journal-title":"Brain Res."},{"issue":"2","key":"10.1016\/j.nbd.2017.09.004_bb0385","doi-asserted-by":"crossref","first-page":"1746-63","DOI":"10.1111\/ejn.12932","article-title":"Methods for studying the zebrafish brain: past, present and future","volume":"42","author":"Wyatt","year":"2015","journal-title":"Eur. J. Neurosci."},{"issue":"3","key":"10.1016\/j.nbd.2017.09.004_bb0390","doi-asserted-by":"crossref","first-page":"445-54","DOI":"10.1093\/hmg\/ddq491","article-title":"Tsc2 Gene inactivation causes a more severe epilepsy phenotype than Tsc1 inactivation in a mouse model of tuberous sclerosis complex","volume":"20","author":"Zeng","year":"2011","journal-title":"Hum. Mol. Genet."},{"issue":"8","key":"10.1016\/j.nbd.2017.09.004_bb0395","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1000604","article-title":"Nuclear calcium signaling controls expression of a large gene pool: identification of a gene program for acquired neuroprotection induced by synaptic activity","volume":"5","author":"Zhang","year":"2009","journal-title":"PLoS Genet."},{"issue":"5","key":"10.1016\/j.nbd.2017.09.004_bb0400","article-title":"Pharmacological characterization of an antisense knockdown zebrafish model of Dravet syndrome: inhibition of epileptic seizures by the serotonin agonist fenfluramine","volume":"10","author":"Zhang","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.nbd.2017.09.004_bb0405","doi-asserted-by":"crossref","DOI":"10.1016\/j.nbd.2015.04.016","article-title":"Inflammatory mechanisms contribute to the neurological manifestations of tuberous sclerosis complex","volume":"80","author":"Zhang","year":"2015","journal-title":"Neurobiol. Dis."},{"issue":"1","key":"10.1016\/j.nbd.2017.09.004_bb0410","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/nrd1606","article-title":"In vivo drug discovery in the zebrafish","volume":"4","author":"Zon","year":"2005","journal-title":"Nat. Rev. Drug Discov."}],"container-title":["Neurobiology of Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969996117302127?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969996117302127?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T16:28:59Z","timestamp":1750868939000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0969996117302127"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":82,"alternative-id":["S0969996117302127"],"URL":"https:\/\/doi.org\/10.1016\/j.nbd.2017.09.004","relation":{},"ISSN":["0969-9961"],"issn-type":[{"value":"0969-9961","type":"print"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"mTOR-related neuropathology in mutant tsc2 zebrafish: Phenotypic, transcriptomic and pharmacological analysis","name":"articletitle","label":"Article Title"},{"value":"Neurobiology of Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.nbd.2017.09.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}