{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T14:29:48Z","timestamp":1792074588491,"version":"4.4.0"},"reference-count":94,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:00:00Z","timestamp":1758844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["U01-AG082665"],"award-info":[{"award-number":["U01-AG082665"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["U01-AG058654"],"award-info":[{"award-number":["U01-AG058654"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["U01-AG062602"],"award-info":[{"award-number":["U01-AG062602"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01-AG048927"],"award-info":[{"award-number":["R01-AG048927"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["P30-AG072978"],"award-info":[{"award-number":["P30-AG072978"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["U01-AG0772577"],"award-info":[{"award-number":["U01-AG0772577"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["U01-081230"],"award-info":[{"award-number":["U01-081230"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Neurobiology of Disease"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.nbd.2025.107128","type":"journal-article","created":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T22:29:56Z","timestamp":1759012196000},"page":"107128","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Single nucleus RNA sequencing unveils relationship between microglia and endothelial cells in mixed Alzheimer's disease and vascular pathology"],"prefix":"10.1016","volume":"216","author":[{"given":"Oluwatosin A.","family":"Olayinka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"O\u2019Neill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny A.","family":"Empawi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Payton","family":"Bock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junming","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Rickner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thor D.","family":"Stein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Wolozin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay A.","family":"Farrer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.nbd.2025.107128_bb0005","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1161\/CIRCULATIONAHA.108.841502","article-title":"Response gene to complement 32, a novel hypoxia-regulated angiogenic inhibitor","volume":"120","author":"An","year":"2009","journal-title":"Circulation"},{"key":"10.1016\/j.nbd.2025.107128_bb0010","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/s41590-018-0276-y","article-title":"Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage","volume":"20","author":"Aran","year":"2019","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.nbd.2025.107128_bb0015","series-title":"SingleR: Reference-Based Single-Cell RNA-Seq Annotation","author":"Aran","year":"2023"},{"key":"10.1016\/j.nbd.2025.107128_bb0020","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s00401-020-02255-2","article-title":"Neuropathological consensus criteria for the evaluation of Lewy pathology in post-mortem brains: a multi-Centre study","volume":"141","author":"Attems","year":"2021","journal-title":"Acta Neuropathol. (Berl.)"},{"key":"10.1016\/j.nbd.2025.107128_bb0025","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1093\/bioinformatics\/btz698","article-title":"Scds: computational annotation of doublets in single-cell RNA sequencing data","volume":"36","author":"Bais","year":"2020","journal-title":"Bioinforma. Oxf. Engl."},{"key":"10.1016\/j.nbd.2025.107128_bb0030","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1038\/s44161-021-00014-4","article-title":"Blood-brain barrier link to human cognitive impairment and Alzheimer's disease","volume":"1","author":"Barisano","year":"2022","journal-title":"Nat. Cardiovasc. Res."},{"key":"10.1016\/j.nbd.2025.107128_bb0035","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1038\/s41588-022-01024-z","article-title":"New insights into the genetic etiology of Alzheimer's disease and related dementias","volume":"54","author":"Bellenguez","year":"2022","journal-title":"Nat. Genet."},{"key":"10.1016\/j.nbd.2025.107128_bb0040","doi-asserted-by":"crossref","first-page":"5289","DOI":"10.1038\/s41467-021-25590-8","article-title":"Capillary-associated microglia regulate vascular structure and function through PANX1-P2RY12 coupling in mice","volume":"12","author":"Bisht","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.nbd.2025.107128_bb0045","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1111\/epi.12220","article-title":"International consensus classification of hippocampal sclerosis in temporal lobe epilepsy: A task force report from the ILAE commission on diagnostic methods","volume":"54","author":"Bl\u00fcmcke","year":"2013","journal-title":"Epilepsia"},{"key":"10.1016\/j.nbd.2025.107128_bb0050","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.nbd.2025.107128_bb0055","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1002\/ana.25123","article-title":"Person-specific contribution of neuropathologies to cognitive loss in old age","volume":"83","author":"Boyle","year":"2018","journal-title":"Ann. Neurol."},{"key":"10.1016\/j.nbd.2025.107128_bb0060","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s00401-018-1928-6","article-title":"Dissecting the genetic relationship between cardiovascular risk factors and Alzheimer's disease","volume":"137","author":"Broce","year":"2019","journal-title":"Acta Neuropathol. (Berl.)"},{"key":"10.1016\/j.nbd.2025.107128_bb0065","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/nbt.4096","article-title":"Integrating single-cell transcriptomic data across different conditions, technologies, and species","volume":"36","author":"Butler","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.nbd.2025.107128_bb0070","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1080\/19336918.2019.1629224","article-title":"Sirtuin 3 promotes microglia migration by upregulating CX3CR1","volume":"13","author":"Cao","year":"2019","journal-title":"Cell Adhes. Migr."},{"key":"10.1016\/j.nbd.2025.107128_bb0075","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1186\/s13578-020-00474-4","article-title":"Interaction of tau with the chemokine receptor, CX3CR1 and its effect on microglial activation, migration and proliferation","volume":"10","author":"Chidambaram","year":"2020","journal-title":"Cell Biosci."},{"key":"10.1016\/j.nbd.2025.107128_bb0080","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1186\/1476-4598-9-138","article-title":"Anti-angiogenic SPARC peptides inhibit progression of neuroblastoma tumors","volume":"9","author":"Chlenski","year":"2010","journal-title":"Mol. Cancer"},{"key":"10.1016\/j.nbd.2025.107128_bb0085","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.neuron.2019.05.013","article-title":"Regional heterogeneity in gene expression, regulation, and coherence in the frontal cortex and Hippocampus across development and schizophrenia","volume":"103","author":"Collado-Torres","year":"2019","journal-title":"Neuron"},{"key":"10.1016\/j.nbd.2025.107128_bb0090","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.nbd.2013.02.003","article-title":"Blockage of CR1 prevents activation of rodent microglia","volume":"54","author":"Crehan","year":"2013","journal-title":"Neurobiol. Dis."},{"key":"10.1016\/j.nbd.2025.107128_bb0095","doi-asserted-by":"crossref","DOI":"10.1084\/jem.20211071","article-title":"Microglia modulate blood flow, neurovascular coupling, and hypoperfusion via purinergic actions","volume":"219","author":"Cs\u00e1sz\u00e1r","year":"2022","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.nbd.2025.107128_bb0100","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/j.cell.2018.05.003","article-title":"Disease-associated microglia: a universal immune sensor of neurodegeneration","volume":"173","author":"Deczkowska","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.nbd.2025.107128_bb0105","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/s41587-020-0465-8","article-title":"Systematic comparison of single-cell and single-nucleus RNA-sequencing methods","volume":"38","author":"Ding","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.nbd.2025.107128_bb0110","doi-asserted-by":"crossref","first-page":"894","DOI":"10.2174\/1567205013666160129095924","article-title":"Glial cells - the key elements of Alzheimer's disease","volume":"13","author":"Dzamba","year":"2016","journal-title":"Curr. Alzheimer Res."},{"key":"10.1016\/j.nbd.2025.107128_bb0115","doi-asserted-by":"crossref","DOI":"10.1007\/s10456-023-09870-z","article-title":"The vascular gene Apold1 is dispensable for normal development but controls angiogenesis under pathological conditions","author":"Fan","year":"2023","journal-title":"Angiogenesis"},{"key":"10.1016\/j.nbd.2025.107128_bb0120","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1007\/s00401-021-02343-x","article-title":"Cross-platform transcriptional profiling identifies common and distinct molecular pathologies in Lewy body diseases","volume":"142","author":"Feleke","year":"2021","journal-title":"Acta Neuropathol. (Berl.)"},{"key":"10.1016\/j.nbd.2025.107128_bb0125","doi-asserted-by":"crossref","first-page":"2593","DOI":"10.1007\/s12035-021-02641-1","article-title":"ADAMTS9-AS2 promotes angiogenesis of brain microvascular endothelial cells through regulating miR-185-5p\/IGFBP-2 Axis in ischemic stroke","volume":"59","author":"Feng","year":"2022","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.nbd.2025.107128_bb0130","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1186\/s13059-015-0844-5","article-title":"MAST: a flexible statistical framework for assessing transcriptional changes and characterizing heterogeneity in single-cell RNA sequencing data","volume":"16","author":"Finak","year":"2015","journal-title":"Genome Biol."},{"key":"10.1016\/j.nbd.2025.107128_bb0135","series-title":"Cell-Subtype Specific Effects of Genetic Variation in the Aging and Alzheimer Cortex","author":"Fujita","year":"2022"},{"key":"10.1016\/j.nbd.2025.107128_bb0140","doi-asserted-by":"crossref","DOI":"10.3389\/fnins.2022.949129","article-title":"Contribution of blood-brain barrier-related blood-borne factors for Alzheimer's disease vs. vascular dementia diagnosis: a pilot study","volume":"16","author":"Gong","year":"2022","journal-title":"Front. Neurosci."},{"key":"10.1016\/j.nbd.2025.107128_bb0145","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1001\/jama.2017.3090","article-title":"Association between midlife vascular risk factors and estimated brain amyloid deposition","volume":"317","author":"Gottesman","year":"2017","journal-title":"JAMA"},{"key":"10.1016\/j.nbd.2025.107128_bb0150","doi-asserted-by":"crossref","first-page":"19802","DOI":"10.1073\/pnas.1319700110","article-title":"RNA-sequencing from single nuclei","volume":"110","author":"Grindberg","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.nbd.2025.107128_bb0155","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1038\/s41593-019-0539-4","article-title":"A single-cell atlas of entorhinal cortex from individuals with Alzheimer's disease reveals cell-type-specific gene expression regulation","volume":"22","author":"Grubman","year":"2019","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.nbd.2025.107128_bb0160","doi-asserted-by":"crossref","first-page":"7551","DOI":"10.1021\/bi401034q","article-title":"Mechanistic basis for the potent anti-angiogenic activity of semaphorin 3F","volume":"52","author":"Guo","year":"2013","journal-title":"Biochemistry"},{"key":"10.1016\/j.nbd.2025.107128_bb0165","doi-asserted-by":"crossref","DOI":"10.1093\/neuonc\/noad179.0385","article-title":"DDEL-06. Microglia repair the disrupted blood-brain barrier following low-intensity pulsed ultrasound through p2ry12 signaling","volume":"25","author":"Habashy","year":"2023","journal-title":"Neuro-Oncology"},{"key":"10.1016\/j.nbd.2025.107128_bb0170","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/s41586-024-07185-7","article-title":"APOE4\/4 is linked to damaging lipid droplets in Alzheimer's disease microglia","volume":"628","author":"Haney","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.nbd.2025.107128_bb0175","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1083\/jcb.201709069","article-title":"Microglia in Alzheimer's disease","volume":"217","author":"Hansen","year":"2018","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.nbd.2025.107128_bb0180","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1038\/nn1805","article-title":"The P2Y12 receptor regulates microglial activation by extracellular nucleotides","volume":"9","author":"Haynes","year":"2006","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.nbd.2025.107128_bb0185","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1038\/s41467-022-29212-9","article-title":"Comprehensive generation, visualization, and reporting of quality control metrics for single-cell RNA sequencing data","volume":"13","author":"Hong","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.nbd.2025.107128_bb0190","first-page":"15","article-title":"Blood\u2013brain barrier breakdown: an emerging biomarker of cognitive impairment in normal aging and dementia","author":"Hussain","year":"2021","journal-title":"Front. Neurosci."},{"key":"10.1016\/j.nbd.2025.107128_bb0195","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1038\/s41588-018-0311-9","article-title":"Genome-wide meta-analysis identifies new loci and functional pathways influencing Alzheimer's disease risk","volume":"51","author":"Jansen","year":"2019","journal-title":"Nat. Genet."},{"key":"10.1016\/j.nbd.2025.107128_bb0200","doi-asserted-by":"crossref","first-page":"2507","DOI":"10.1182\/blood.V128.22.2507.2507","article-title":"Fractalkine\/CX3CR1 signaling promotes angiogenic potentials in CX3CR1 expressing monocytes","volume":"128","author":"Kim","year":"2016","journal-title":"Blood"},{"key":"10.1016\/j.nbd.2025.107128_bb0205","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/19768354.2018.1508498","article-title":"Exploring glia to better understand Alzheimer's disease","volume":"22","author":"Kim","year":"2018","journal-title":"Anim. Cells Syst."},{"key":"10.1016\/j.nbd.2025.107128_bb0210","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.2353\/ajpath.2010.090655","article-title":"ADAMTS9 is a cell-autonomously acting, anti-angiogenic metalloprotease expressed by microvascular endothelial cells","volume":"176","author":"Koo","year":"2010","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.nbd.2025.107128_bb0215","series-title":"StatPearls","article-title":"Alzheimer disease","author":"Kumar","year":"2023"},{"key":"10.1016\/j.nbd.2025.107128_bb0220","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1038\/s41588-019-0358-2","article-title":"Genetic meta-analysis of diagnosed Alzheimer's disease identifies new risk loci and implicates A\u03b2, tau, immunity and lipid processing","volume":"51","author":"Kunkle","year":"2019","journal-title":"Nat. Genet."},{"key":"10.1016\/j.nbd.2025.107128_bb0225","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1186\/1471-2105-9-559","article-title":"WGCNA: an R package for weighted correlation network analysis","volume":"9","author":"Langfelder","year":"2008","journal-title":"BMC Bioinform."},{"key":"10.1016\/j.nbd.2025.107128_bb0230","doi-asserted-by":"crossref","first-page":"25800","DOI":"10.1073\/pnas.2008762117","article-title":"Single-nucleus transcriptome analysis reveals dysregulation of angiogenic endothelial cells and neuroprotective glia in Alzheimer's disease","volume":"117","author":"Lau","year":"2020","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.nbd.2025.107128_bb0235","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1000217","article-title":"Inferring pathway activity toward precise disease classification","volume":"4","author":"Lee","year":"2008","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.nbd.2025.107128_bb0240","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2023.112196","article-title":"APOE modulates microglial immunometabolism in response to age, amyloid pathology, and inflammatory challenge","volume":"42","author":"Lee","year":"2023","journal-title":"Cell Rep."},{"key":"10.1016\/j.nbd.2025.107128_bb0245","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/s13024-017-0192-x","article-title":"Glial contributions to neurodegeneration in tauopathies","volume":"12","author":"Leyns","year":"2017","journal-title":"Mol. Neurodegener."},{"key":"10.1016\/j.nbd.2025.107128_bb0250","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1186\/1471-2105-12-323","article-title":"RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome","volume":"12","author":"Li","year":"2011","journal-title":"BMC Bioinform."},{"key":"10.1016\/j.nbd.2025.107128_bb0255","doi-asserted-by":"crossref","first-page":"5567","DOI":"10.1158\/0008-5472.CAN-09-4510","article-title":"Extracellular protease ADAMTS9 suppresses esophageal and nasopharyngeal carcinoma tumor formation by inhibiting angiogenesis","volume":"70","author":"Lo","year":"2010","journal-title":"Cancer Res."},{"key":"10.1016\/j.nbd.2025.107128_bb0260","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1073\/pnas.1520398113","article-title":"Purinergic receptor P2RY12-dependent microglial closure of the injured blood-brain barrier","volume":"113","author":"Lou","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.nbd.2025.107128_bb0265","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1186\/s12915-021-01071-8","article-title":"Single-cell spatial transcriptomic analysis reveals common and divergent features of developing postnatal granule cerebellar cells and medulloblastoma","volume":"19","author":"Luo","year":"2021","journal-title":"BMC Biol."},{"key":"10.1016\/j.nbd.2025.107128_bb0270","doi-asserted-by":"crossref","DOI":"10.1093\/eurjpc\/zwaf129","article-title":"Cardiovascular diseases and risk of dementia in the general population","author":"Luo","year":"2025","journal-title":"Eur. J. Prev. Cardiol."},{"key":"10.1016\/j.nbd.2025.107128_bb0275","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1111\/j.1471-4159.2009.06253.x","article-title":"Fractalkine-induced activation of the phosphatidylinositol-3 kinase pathway attentuates microglial activation in vivo and in vitro","volume":"110","author":"Lyons","year":"2009","journal-title":"J. Neurochem."},{"key":"10.1016\/j.nbd.2025.107128_bb0280","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1038\/s41586-019-1195-2","article-title":"Single-cell transcriptomic analysis of Alzheimer's disease","volume":"570","author":"Mathys","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.nbd.2025.107128_bb0285","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1038\/s41586-024-07606-7","article-title":"Single-cell multiregion dissection of Alzheimer's disease","volume":"632","author":"Mathys","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.nbd.2025.107128_bb0290","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1002\/glia.23097","article-title":"P2Y12 receptor is expressed on human microglia under physiological conditions throughout development and is sensitive to neuroinflammatory diseases","volume":"65","author":"Mildner","year":"2017","journal-title":"Glia"},{"key":"10.1016\/j.nbd.2025.107128_bb0295","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.1111\/jcmm.17315","article-title":"Single-nucleus transcriptome analysis reveals disease- and regeneration-associated endothelial cells in white matter vascular dementia","volume":"26","author":"Mitroi","year":"2022","journal-title":"J. Cell. Mol. Med."},{"key":"10.1016\/j.nbd.2025.107128_bb0300","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1038\/s41588-021-00894-z","article-title":"Single-nucleus chromatin accessibility and transcriptomic characterization of Alzheimer's disease","volume":"53","author":"Morabito","year":"2021","journal-title":"Nat. Genet."},{"key":"10.1016\/j.nbd.2025.107128_bb0305","doi-asserted-by":"crossref","first-page":"1546","DOI":"10.1212\/WNL.51.6.1546","article-title":"Frontotemporal lobar degeneration: a consensus on clinical diagnostic criteria","volume":"51","author":"Neary","year":"1998","journal-title":"Neurology"},{"key":"10.1016\/j.nbd.2025.107128_bb0310","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a006718","article-title":"Semaphorins in angiogenesis and tumor progression","volume":"2","author":"Neufeld","year":"2012","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"10.1016\/j.nbd.2025.107128_bb0315","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1097\/00005072-199911000-00004","article-title":"Application of the National Institute on Aging (NIA)-Reagan institute criteria for the neuropathological diagnosis of Alzheimer disease","volume":"58","author":"Newell","year":"1999","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"10.1016\/j.nbd.2025.107128_bb0320","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s12974-019-1399-2","article-title":"Glial activation and inflammation along the Alzheimer's disease continuum","volume":"16","author":"Nordengen","year":"2019","journal-title":"J. Neuroinflammation"},{"key":"10.1016\/j.nbd.2025.107128_bb0325","doi-asserted-by":"crossref","first-page":"6129","DOI":"10.1038\/s41467-020-19737-2","article-title":"Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease","volume":"11","author":"Olah","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.nbd.2025.107128_bb0330","doi-asserted-by":"crossref","first-page":"W193","DOI":"10.1093\/nar\/gkm226","article-title":"G:profiler\u2014a web-based toolset for functional profiling of gene lists from large-scale experiments","volume":"35","author":"Reimand","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.nbd.2025.107128_bb0335","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0145584","article-title":"Serglycin in quiescent and proliferating primary endothelial cells","volume":"10","author":"Reine","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.nbd.2025.107128_bb0340","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.nbd.2025.107128_bb0345","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/cr.2011.198","article-title":"Semaphorin signaling in angiogenesis, lymphangiogenesis and cancer","volume":"22","author":"Sakurai","year":"2012","journal-title":"Cell Res."},{"key":"10.1016\/j.nbd.2025.107128_bb0350","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.jalz.2009.04.1233","article-title":"Cerebral blood flow in ischemic vascular dementia and Alzheimer's disease, measured by arterial spin-labeling magnetic resonance imaging","volume":"5","author":"Schuff","year":"2009","journal-title":"Alzheimers Dement. J. Alzheimers Assoc."},{"key":"10.1016\/j.nbd.2025.107128_bb0355","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1084\/jem.20171529","article-title":"Humanized TREM2 mice reveal microglia-intrinsic and -extrinsic effects of R47H polymorphism","volume":"215","author":"Song","year":"2018","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.nbd.2025.107128_bb0360","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1002\/alz.12343","article-title":"Association of cardiovascular risk burden with risk of dementia and brain pathologies: a population-based cohort study","volume":"17","author":"Song","year":"2021","journal-title":"Alzheimers Dement."},{"key":"10.1016\/j.nbd.2025.107128_bb0365","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1073\/pnas.1714446115","article-title":"Context-dependent functions of angiopoietin 2 are determined by the endothelial phosphatase VEPTP","volume":"115","author":"Souma","year":"2018","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.nbd.2025.107128_bb0370","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bbi.2016.07.003","article-title":"Inflammation in Alzheimer's disease: lessons learned from microglia-depletion models","volume":"61","author":"Spangenberg","year":"2017","journal-title":"Brain Behav. Immun."},{"key":"10.1016\/j.nbd.2025.107128_bb0375","series-title":"STAR: Ultrafast Universal RNA-Seq Aligner","year":"2025"},{"key":"10.1016\/j.nbd.2025.107128_bb0380","doi-asserted-by":"crossref","first-page":"772","DOI":"10.3324\/haematol.2022.280816","article-title":"APOLD1 loss causes endothelial dysfunction involving cell junctions, cytoskeletal architecture, and Weibel-Palade bodies, while disrupting hemostasis","volume":"108","author":"Stritt","year":"2023","journal-title":"Haematologica"},{"key":"10.1016\/j.nbd.2025.107128_bb0385","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1093\/jnci\/djab183","article-title":"SRGN-triggered aggressive and immunosuppressive phenotype in a subset of TTF-1-negative lung adenocarcinomas","volume":"114","author":"Tanaka","year":"2022","journal-title":"J. Natl. Cancer Inst."},{"key":"10.1016\/j.nbd.2025.107128_bb0390","article-title":"Mediators of cerebral hypoperfusion and blood-brain barrier leakiness in Alzheimer's disease, vascular dementia and mixed dementia","volume":"31","author":"Tayler","year":"2021","journal-title":"Brain Pathol. Zurich Switz."},{"key":"10.1016\/j.nbd.2025.107128_bb0395","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2020.108189","article-title":"Single-nucleus RNA-Seq is not suitable for detection of microglial activation genes in humans","volume":"32","author":"Thrupp","year":"2020","journal-title":"Cell Rep."},{"key":"10.1016\/j.nbd.2025.107128_bb0400","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/s41380-018-0036-2","article-title":"The role of adult hippocampal neurogenesis in brain health and disease","volume":"24","author":"Toda","year":"2019","journal-title":"Mol. Psychiatry"},{"key":"10.1016\/j.nbd.2025.107128_bb0405","doi-asserted-by":"crossref","DOI":"10.1016\/j.arr.2022.101622","article-title":"Glial cells in Alzheimer's disease: from neuropathological changes to therapeutic implications","volume":"78","author":"Uddin","year":"2022","journal-title":"Ageing Res. Rev."},{"key":"10.1016\/j.nbd.2025.107128_bb0410","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1111\/neup.12262","article-title":"Blood-brain barrier damage in vascular dementia","volume":"36","author":"Ueno","year":"2016","journal-title":"Neuropathol. Off. J. Jpn. Soc. Neuropathol."},{"key":"10.1016\/j.nbd.2025.107128_bb0415","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1161\/CIRCRESAHA.120.318380","article-title":"Perivascular macrophages regulate blood flow following tissue damage","volume":"128","author":"V\u00e5gesj\u00f6","year":"2021","journal-title":"Circ. Res."},{"key":"10.1016\/j.nbd.2025.107128_bb0420","first-page":"84","article-title":"Vascular endothelial protein tyrosine phosphatase regulates endothelial function","volume":"36","author":"Vestweber","year":"2021","journal-title":"Physiol. Bethesda Md."},{"key":"10.1016\/j.nbd.2025.107128_bb0425","doi-asserted-by":"crossref","DOI":"10.3389\/fnsyn.2021.773590","article-title":"Microglia heterogeneity in Alzheimer's disease: insights from single-cell technologies","volume":"13","author":"Wang","year":"2021","journal-title":"Front. Synaptic Neurosci."},{"key":"10.1016\/j.nbd.2025.107128_bb0430","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1038\/s41588-021-00921-z","article-title":"A genome-wide association study with 1,126,563 individuals identifies new risk loci for Alzheimer's disease","volume":"53","author":"Wightman","year":"2021","journal-title":"Nat. Genet."},{"key":"10.1016\/j.nbd.2025.107128_bb0435","first-page":"7","article-title":"Microglia, seen from the CX3CR1 angle","author":"Wolf","year":"2013","journal-title":"Front. Cell. Neurosci."},{"key":"10.1016\/j.nbd.2025.107128_bb0440","doi-asserted-by":"crossref","DOI":"10.1161\/CIRCULATIONAHA.117.027448","article-title":"Cerebral perfusion and the risk of dementia","author":"Wolters","year":"2017","journal-title":"Circulation"},{"key":"10.1016\/j.nbd.2025.107128_bb0445","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1681\/ASN.2018090912","article-title":"Advantages of single-nucleus over single-cell RNA sequencing of adult kidney: rare cell types and novel cell states revealed in fibrosis","volume":"30","author":"Wu","year":"2019","journal-title":"J. Am. Soc. Nephrol. JASN"},{"key":"10.1016\/j.nbd.2025.107128_bb0450","doi-asserted-by":"crossref","DOI":"10.1002\/ctm2.1031","article-title":"Serglycin-induced interleukin-1\u03b2 from oesophageal cancer cells upregulate hepatocyte growth factor in fibroblasts to promote tumour angiogenesis and growth","volume":"12","author":"Yan","year":"2022","journal-title":"Clin. Transl. Med."},{"key":"10.1016\/j.nbd.2025.107128_bb0455","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s13059-020-1950-6","article-title":"Decontamination of ambient RNA in single-cell RNA-seq with DecontX","volume":"21","author":"Yang","year":"2020","journal-title":"Genome Biol."},{"key":"10.1016\/j.nbd.2025.107128_bb0460","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1038\/s41586-021-04369-3","article-title":"A human brain vascular atlas reveals diverse mediators of Alzheimer's risk","volume":"603","author":"Yang","year":"2022","journal-title":"Nature"},{"key":"10.1016\/j.nbd.2025.107128_bb0465","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1038\/s41591-019-0695-9","article-title":"Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease","volume":"26","author":"Zhou","year":"2020","journal-title":"Nat. Med."},{"key":"10.1016\/j.nbd.2025.107128_bb0470","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1002\/(SICI)1098-1136(20000215)29:4<305::AID-GLIA2>3.0.CO;2-V","article-title":"Fractalkine modulates TNF-alpha secretion and neurotoxicity induced by microglial activation","volume":"29","author":"Zujovic","year":"2000","journal-title":"Glia"}],"container-title":["Neurobiology of Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969996125003456?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969996125003456?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T21:02:58Z","timestamp":1778792578000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0969996125003456"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":94,"alternative-id":["S0969996125003456"],"URL":"https:\/\/doi.org\/10.1016\/j.nbd.2025.107128","relation":{},"ISSN":["0969-9961"],"issn-type":[{"value":"0969-9961","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Single nucleus RNA sequencing unveils relationship between microglia and endothelial cells in mixed Alzheimer's disease and vascular pathology","name":"articletitle","label":"Article Title"},{"value":"Neurobiology of Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.nbd.2025.107128","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"107128"}}