{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:39:59Z","timestamp":1789180799225,"version":"build-2803163510"},"reference-count":45,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["New Biotechnology"],"published-print":{"date-parts":[[2012,11]]},"DOI":"10.1016\/j.nbt.2012.07.006","type":"journal-article","created":{"date-parts":[[2012,7,23]],"date-time":"2012-07-23T21:02:01Z","timestamp":1343077321000},"page":"23-32","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":27,"title":["Field distribution and activity of chlorinated solvents degrading bacteria by combining CARD-FISH and real time PCR"],"prefix":"10.1016","volume":"30","author":[{"given":"B.","family":"Matturro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Aulenta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Majone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Petrangeli","family":"Papini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Tandoi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Rossetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.nbt.2012.07.006_bib0005","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1146\/annurev.micro.58.030603.123600","article-title":"Anaerobic microbial dehalogenation","volume":"58","author":"Smidt","year":"2004","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0010","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1128\/AEM.69.2.996-1003.2003","article-title":"Complete detoxification of vinyl chloride by anaerobic enrichment culture and identification of the reductively dechlorinating population as \u201cDehalococcoides\u201d species","volume":"69","author":"He","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0015","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1126\/science.276.5318.1568","article-title":"Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene","volume":"276","author":"Maymo-Gatell","year":"1997","journal-title":"Science"},{"key":"10.1016\/j.nbt.2012.07.006_bib0020","doi-asserted-by":"crossref","first-page":"4880","DOI":"10.1128\/AEM.70.8.4880-4888.2004","article-title":"Molecular identification of the catabolic vinyl chloride reductase from \u201cDehalococcoides\u201d sp. strain VS and its environmental distribution","volume":"70","author":"Muller","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0025","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1128\/AEM.72.3.1980-1987.2006","article-title":"Quantitative PCR confirms purity of strain GT, a novel trichloroethene (TCE)-to-ethene-respiring \u201cDehalococcoides\u201d isolate","volume":"72","author":"Sung","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0030","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1038\/35046063","article-title":"Bacterial dehalorespiration with chlorinated benzenes","volume":"408","author":"Adrian","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.nbt.2012.07.006_bib0035","doi-asserted-by":"crossref","first-page":"3866","DOI":"10.1128\/AEM.71.7.3866-3871.2005","article-title":"An internal reference technique for accurately quantifying specific mRNAs by real-time PCR with application to the tceA reductive dehalogenase gene","volume":"71","author":"Johnson","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0040","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0167-7012(01)00370-0","article-title":"Enumeration of total bacteria and bacteria with genes for proteolytic activity in pure cultures and in environmental samples by quantitative PCR mediated amplification","volume":"49","author":"Bach","year":"2002","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.nbt.2012.07.006_bib0045","first-page":"13","article-title":"Analysis of specific bacteria from environmental samples using a quantitative polymerase chain reaction","volume":"4","author":"Brunk","year":"2002","journal-title":"Curr. Issues Mol. Biol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0050","doi-asserted-by":"crossref","first-page":"1442","DOI":"10.1111\/j.1462-2920.2005.00830.x","article-title":"Isolation and characterization of \u201cDehalococcoides\u201d sp. strain FL2, a trichloroethene (TCE) and 1,2-dichloroethene-respiring anaerobe","volume":"7","author":"He","year":"2005","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0055","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1111\/j.1198-743X.2004.00722.x","article-title":"Real-time PCR in the microbiology laboratory","volume":"10","author":"Mackay","year":"2004","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.nbt.2012.07.006_bib0060","doi-asserted-by":"crossref","first-page":"5141","DOI":"10.1128\/AEM.66.12.5141-5147.2000","article-title":"Trichloroethene reductive dehalogenase from \u201cDehalococcoides\u201d ethenogenes: sequence of tceA and substrate range characterization","volume":"66","author":"Magnuson","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0065","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1126\/science.1102226","article-title":"Genome sequence of the PCE-dechlorinating bacterium \u201cDehalococcoides\u201d ethenogenes","volume":"307","author":"Seshadri","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.nbt.2012.07.006_bib0070","first-page":"347","article-title":"Molecular techniques in the biotechnological fight against halogenated compounds in anoxic environments","volume":"3","author":"Ding","year":"2011","journal-title":"Microbial Biotechnol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0075","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mimet.2007.11.005","article-title":"Real-time PCR quantification of \u201cDehalococcoides\u201d populations: methods and applications","volume":"72","author":"Cupples","year":"2008","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.nbt.2012.07.006_bib0080","doi-asserted-by":"crossref","first-page":"2765","DOI":"10.1128\/AEM.72.4.2765-2774.2006","article-title":"Quantitative PCR targeting 16S rRNA and reductive dehalogenase genes simultaneously monitors multiple \u201cDehalococcoides\u201d strains","volume":"72","author":"Ritalahti","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0085","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1080\/10889860802477218","article-title":"Detection and quantification of Dehalococcoides-related bacteria in a chlorinated ethene-contaminated aquifer undergoing natural attenuation","volume":"12","author":"B\u00fcrgmann","year":"2008","journal-title":"Biorem. J."},{"key":"10.1016\/j.nbt.2012.07.006_bib0090","doi-asserted-by":"crossref","first-page":"4301","DOI":"10.1021\/es803308q","article-title":"Assessment of in situ reductive dechlorination using compound-specific stable isotopes, functional gene PCR, and geochemical data","volume":"43","author":"Carre\u00f3n-Diazconti","year":"2009","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0095","doi-asserted-by":"crossref","first-page":"2728","DOI":"10.1128\/AEM.02199-07","article-title":"Transcripts to assess the in situ physiology of \u201cDehalococcoides\u201d spp. in a trichloroethene-contaminated groundwater site","volume":"74","author":"Lee","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0100","doi-asserted-by":"crossref","first-page":"3131","DOI":"10.1016\/j.watres.2006.05.030","article-title":"Relationship between \u201cDehalococcoides\u201d DNA in ground water and rates of reductive dechlorination at field scale","volume":"40","author":"Lu","year":"2006","journal-title":"Water Res."},{"key":"10.1016\/j.nbt.2012.07.006_bib0105","doi-asserted-by":"crossref","first-page":"5127","DOI":"10.1021\/es100408r","article-title":"Comparing on-site to off-site biomass collection for \u201cDehalococcoides\u201d biomarker gene quantification to predict in situ chlorinated ethene detoxification potential","volume":"13","author":"Ritalahti","year":"2010","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0110","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1007\/s00253-003-1503-4","article-title":"Detection and quantitative estimation of \u201cDehalococcoides\u201d spp. in a dechlorinating bioreactor by a combination of fluorescent in situ hybridisation (FISH) and kinetic analysis","volume":"64","author":"Aulenta","year":"2004","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0115","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.syapm.2007.11.001","article-title":"Improved quantification of \u201cDehalococcoides\u201d species by fluorescence in situ hybridization and catalyzed reporter deposition","volume":"31","author":"Fazi","year":"2008","journal-title":"Syst. Appl. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0120","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1002\/bit.21776","article-title":"Structure analysis and performance of a microbial community from a contaminated aquifer involved in the complete reductive dechlorination of 1,1,2,2-tetrachloroethane to ethene","volume":"100","author":"Rossetti","year":"2008","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.nbt.2012.07.006_bib0125","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1099\/mic.0.27819-0","article-title":"A PCR-based specific assay reveals a population of bacteria within the Chloroflexi associated with the reductive dehalogenation of polychlorinated biphenyls","volume":"151","author":"Watts","year":"2005","journal-title":"Microbiology"},{"key":"10.1016\/j.nbt.2012.07.006_bib0130","first-page":"1513","article-title":"Modelling the limit of detection in real-time quantitative PCR","volume":"226","author":"Burns","year":"2008","journal-title":"Res. Technol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0135","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/nrmicro1888","article-title":"Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques","volume":"6","author":"Amann","year":"2008","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0140","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1002\/bit.20569","article-title":"Comparative study of methanol, butyrate, and hydrogen as electron donors for long-term dechlorination of tetrachloroethene in mixed anaerobic cultures","volume":"91","author":"Aulenta","year":"2005","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.nbt.2012.07.006_bib0145","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1002\/bit.22234","article-title":"Microbial reductive dechlorination of trichloroethene to ethene with electrodes serving as electron donors without the external addition of redox mediators","volume":"103","author":"Aulenta","year":"2009","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.nbt.2012.07.006_bib0150","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1016\/j.bios.2009.12.033","article-title":"Characterization of an electro-active biocathode capable of dechlorinating trichloroethene and cis-dichloroethene to ethane","volume":"25","author":"Aulenta","year":"2010","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.nbt.2012.07.006_bib0155","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.watres.2005.07.010","article-title":"Identification of microorganisms involved in reductive dehalogenation of chlorinated ethenes in an anaerobic microbial community","volume":"39","author":"Yang","year":"2005","journal-title":"Water Res."},{"key":"10.1016\/j.nbt.2012.07.006_bib0160","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s10532-005-4218-7","article-title":"Anaerobic bioremediation of groundwater containing a mixture of 1,1,2,2-tetrachloroethane and chloroethenes","volume":"17","author":"Aulenta","year":"2006","journal-title":"Biodegradation"},{"key":"10.1016\/j.nbt.2012.07.006_bib0165","doi-asserted-by":"crossref","first-page":"3009","DOI":"10.1128\/AEM.02958-06","article-title":"Microbial reductive dechlorination of Aroclor 1260 in Baltimore harbor sediment microcosms is catalyzed by three phylotypes within the phylum Chloroflexi","volume":"73","author":"Fagervold","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0170","doi-asserted-by":"crossref","first-page":"D800","DOI":"10.1093\/nar\/gkl856","article-title":"probeBase \u2013 an online resource for rRNA-targeted oligonucleotide probes: new features 2007","volume":"35","author":"Loy","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.nbt.2012.07.006_bib0175","doi-asserted-by":"crossref","first-page":"4715","DOI":"10.1128\/AEM.65.11.4715-4724.1999","article-title":"Evaluation and optimization of DNA extraction and purification procedures for soil and sediment samples","volume":"65","author":"Miller","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0180","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0580-9517(01)30046-6","article-title":"Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes","volume":"30","author":"Pernthaler","year":"2001","journal-title":"Methods Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0185","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.watres.2006.09.019","article-title":"Relevance of side reactions in anaerobic reductive dechlorination microcosms amended with different electron donors","volume":"41","author":"Aulenta","year":"2007","journal-title":"Water Res."},{"key":"10.1016\/j.nbt.2012.07.006_bib0190","doi-asserted-by":"crossref","first-page":"5943","DOI":"10.1128\/AEM.00961-08","article-title":"Graphite electrode as a sole electron donor for reductive dechlorination of tetrachloroethene by Geobacter lovleyi","volume":"74","author":"Strycharz","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0195","doi-asserted-by":"crossref","first-page":"2775","DOI":"10.1128\/AEM.72.4.2775-2782.2006","article-title":"Geobacter lovleyi sp. nov. strain SZ, a novel metal-reducing and tetrachloroethene \u2013 dechlorinating bacterium","volume":"72","author":"Sung","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0200","doi-asserted-by":"crossref","first-page":"6812","DOI":"10.1021\/ie070048m","article-title":"Field study in situ bioremediation of a chlorinated solvent source zone","volume":"46","author":"Aulenta","year":"2007","journal-title":"Ind. Eng. Chem. Res."},{"key":"10.1016\/j.nbt.2012.07.006_bib41","doi-asserted-by":"crossref","first-page":"7560","DOI":"10.1128\/AEM.01938-09","article-title":"Detection and quantification of Dehalogenimonas and \u201cDehalococcoides\u201d populations via PCR-based protocols targeting 16S rRNA genes","volume":"23","author":"Yan","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0205","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1093\/nar\/29.1.181","article-title":"Rrndb: the ribosomal RNA operon copy number databases","volume":"29","author":"Klappenbach","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.nbt.2012.07.006_bib0210","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.mimet.2004.02.003","article-title":"Development of a real-time PCR method for quantification of the three genera Dehalobacter, Dehalococcoides, and Desulfitobacterium in microbial communities","volume":"57","author":"Smits","year":"2004","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.nbt.2012.07.006_bib0215","doi-asserted-by":"crossref","first-page":"6161","DOI":"10.1128\/AEM.01070-06","article-title":"Reductive dehalogenase gene expression as a biomarker for physiological activity of \u201cDehalococcoides\u201d spp.","volume":"72","author":"Lee","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.nbt.2012.07.006_bib0220","doi-asserted-by":"crossref","first-page":"7145","DOI":"10.1128\/AEM.71.11.7145-7151.2005","article-title":"Transcriptional expression of the tceA gene in a Dehalococcoides-containing microbial enrichment","volume":"71","author":"Johnson","year":"2005","journal-title":"Appl. Environ. Microbiol."}],"container-title":["New Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1871678412001392?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1871678412001392?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T09:52:44Z","timestamp":1761731564000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1871678412001392"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["S1871678412001392"],"URL":"https:\/\/doi.org\/10.1016\/j.nbt.2012.07.006","relation":{},"ISSN":["1871-6784"],"issn-type":[{"value":"1871-6784","type":"print"}],"subject":[],"published":{"date-parts":[[2012,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Field distribution and activity of chlorinated solvents degrading bacteria by combining CARD-FISH and real time PCR","name":"articletitle","label":"Article Title"},{"value":"New Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.nbt.2012.07.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2012 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}