{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T06:20:06Z","timestamp":1777702806217,"version":"3.51.4"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Council for Innovation, Science and Enterprise of the Regional Government of Andalusia","award":["D.P12-TEP-725"],"award-info":[{"award-number":["D.P12-TEP-725"]}]},{"name":"Andalusian Research Groups","award":["CTS-569"],"award-info":[{"award-number":["CTS-569"]}]},{"name":"Andalusian Research Groups","award":["TEP-105"],"award-info":[{"award-number":["TEP-105"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["New Biotechnology"],"published-print":{"date-parts":[[2019,5]]},"DOI":"10.1016\/j.nbt.2019.01.002","type":"journal-article","created":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T23:03:26Z","timestamp":1546988606000},"page":"9-19","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Overexpression of the nitroreductase NfsB in an E. coli strain as a whole-cell biocatalyst for the production of chlorinated analogues of the natural herbicide DIBOA"],"prefix":"10.1016","volume":"50","author":[{"given":"Maria Elena","family":"de la Calle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gema","family":"Cabrera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Domingo","family":"Cantero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4459-4627","authenticated-orcid":false,"given":"Antonio","family":"Valle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Bolivar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.nbt.2019.01.002_bib0005","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1002\/ps.1015","article-title":"Herbicide-resistant crops and weed resistance to herbicides","volume":"61","author":"Owen","year":"2005","journal-title":"Pest Manag Sci"},{"key":"10.1016\/j.nbt.2019.01.002_bib0010","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1046\/j.1365-3180.2000.00166.x","article-title":"EWRS and the challenges for weed research at the start of a new millennium","volume":"40","author":"Kropff","year":"2000","journal-title":"Weed Res"},{"key":"10.1016\/j.nbt.2019.01.002_bib0015","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1046\/j.1365-3180.2000.00174.x","article-title":"The role of ecology in the development of weed management systems: an outlook","volume":"40","author":"Mortensen","year":"2000","journal-title":"Weed Res"},{"key":"10.1016\/j.nbt.2019.01.002_bib0020","doi-asserted-by":"crossref","first-page":"2512","DOI":"10.1021\/jf9903051","article-title":"Search for a standard phytotoxic bioassay for allelochemicals. Selection of standard target species","volume":"48","author":"Mac\u00edas","year":"2000","journal-title":"J Agric Food Chem"},{"key":"10.1016\/j.nbt.2019.01.002_bib0025","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1039\/b700682a","article-title":"Rediscovering the bioactivity and ecological role of 1,4-benzoxazinones","volume":"26","author":"Mac\u00edas","year":"2009","journal-title":"Nat Prod Rep"},{"key":"10.1016\/j.nbt.2019.01.002_bib0030","series-title":"Natural products for pest management","first-page":"2","author":"Rimando","year":"2006"},{"key":"10.1016\/j.nbt.2019.01.002_bib0035","doi-asserted-by":"crossref","first-page":"9843","DOI":"10.1021\/jf062709g","article-title":"New herbicide models from benzoxazinones: Aromatic ring functionalization effects","volume":"54","author":"Mac\u00edas","year":"2006","journal-title":"J Agric Food Chem"},{"key":"10.1016\/j.nbt.2019.01.002_bib0040","doi-asserted-by":"crossref","first-page":"6402","DOI":"10.1021\/jf0488514","article-title":"Degradation studies on benzoxazinoids. soil degradation dynamics of 2,4-dihydroxy-7-methoxy-(2 H)-1,4-benzoxazin-3(4 H)-one (DIMBOA) and its degradation products, phytotoxic allelochemicals from Gramineae","volume":"52","author":"Mac\u00edas","year":"2004","journal-title":"J Agric Food Chem"},{"key":"10.1016\/j.nbt.2019.01.002_bib0045","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.3390\/toxins2081943","article-title":"Natural toxins for use in pest management","volume":"2","author":"Duke","year":"2010","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.nbt.2019.01.002_bib0050","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1080\/07352689991309414","article-title":"Roles of allelopathy in plant biodiversity and sustainable agriculture","volume":"18","author":"Chou","year":"1999","journal-title":"CRC Crit Rev Plant Sci"},{"key":"10.1016\/j.nbt.2019.01.002_bib0055","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1046\/j.1365-3180.2000.00161.x","article-title":"Natural products as sources of herbicides: current status and future trends","volume":"40","author":"Duke","year":"2000","journal-title":"Weed Res"},{"key":"10.1016\/j.nbt.2019.01.002_bib0060","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1021\/jf050896x","article-title":"Isolation and synthesis of allelochemicals from Gramineae: benzoxazinones and related compounds","volume":"54","author":"Mac\u00edas","year":"2006","journal-title":"J Agric Food Chem"},{"key":"10.1016\/j.nbt.2019.01.002_bib0065","doi-asserted-by":"crossref","first-page":"9357","DOI":"10.1021\/jf062168v","article-title":"Optimization of benzoxazinones as natural herbicide models by lipophilicity enhancement","volume":"54","author":"Mac\u00edas","year":"2006","journal-title":"J Agric Food Chem"},{"key":"10.1016\/j.nbt.2019.01.002_bib0070","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1300\/J144v04n02_08","article-title":"The use of allelopathic studies in the search for natural herbicides","volume":"4","author":"Mac\u00edas","year":"2001","journal-title":"J Crop Prod Process"},{"key":"10.1016\/j.nbt.2019.01.002_bib0075","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1021\/jo00005a025","article-title":"Analogs of the cyclic hydroxamic acid 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3-one (DIMBOA): decomposition to benzoxazolinones and reaction with beta-mercaptoethanol","volume":"56","author":"Atkinson","year":"1991","journal-title":"J Org Chem"},{"key":"10.1016\/j.nbt.2019.01.002_bib0080","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.copbio.2016.05.005","article-title":"Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale","volume":"42","author":"Wachtmeister","year":"2016","journal-title":"Curr Opin Biotechnol"},{"key":"10.1016\/j.nbt.2019.01.002_bib0085","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.biotechadv.2010.09.001","article-title":"Enzymatic and whole cell catalysis: finding new strategies for old processes","volume":"29","author":"de Carvalho","year":"2011","journal-title":"Biotechnol Adv"},{"key":"10.1016\/j.nbt.2019.01.002_bib0090","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.procbio.2010.09.011","article-title":"Biotransformation of ethyl 2-(2`-nitrophenoxy)acetate to benzohydroxamic acid (D-DIBOA) by Escherichia coli","volume":"46","author":"Valle","year":"2011","journal-title":"Process Biochem"},{"key":"10.1016\/j.nbt.2019.01.002_bib0095","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s00253-011-3787-0","volume":"94","author":"Valle","year":"2012","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.nbt.2019.01.002_bib0100","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1111\/j.1574-6976.2008.00107.x","article-title":"Reduction of polynitroaromatic compounds: the bacterial nitroreductases","volume":"32","author":"Rold\u00e1n","year":"2008","journal-title":"FEMS Microbiol Rev"},{"key":"10.1016\/j.nbt.2019.01.002_bib0105","series-title":"Molecular cloning : a laboratory manual","author":"Green","year":"2001"},{"key":"10.1016\/j.nbt.2019.01.002_bib0110","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J Biol Chem"},{"key":"10.1016\/j.nbt.2019.01.002_bib0115","series-title":"Standard methods for the examination of water and wastewater","article-title":"American public health association","author":"Greenberg","year":"1992"},{"key":"10.1016\/j.nbt.2019.01.002_bib0120","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1002\/bmb.2005.49403306399","article-title":"SigrafW: an easy-to-use program for fitting enzyme kinetic data","volume":"33","author":"Leone","year":"2005","journal-title":"Biochem Mol Biol Educ"},{"key":"10.1016\/j.nbt.2019.01.002_bib0125","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1016\/S0040-4020(02)00052-2","article-title":"Allelochemicals as leads for new herbicides and agrochemicals","volume":"58","author":"Vyvyan","year":"2002","journal-title":"Tetrahedron"},{"key":"10.1016\/j.nbt.2019.01.002_bib0130","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1021\/op4001675","article-title":"advances in the process development of biocatalytic processes","volume":"17","author":"Tufvesson","year":"2013","journal-title":"Org Process Res Dev"},{"key":"10.1016\/j.nbt.2019.01.002_bib0135","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1186\/s12934-017-0732-7","article-title":"Engineering microbial cell factories for the production of plant natural products: from design principles to industrial-scale production","volume":"16","author":"Liu","year":"2017","journal-title":"Microb Cell Fact"},{"key":"10.1016\/j.nbt.2019.01.002_bib0140","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.tibtech.2008.03.004","article-title":"New opportunities for biocatalysis: making pharmaceutical processes greener","volume":"26","author":"Woodley","year":"2008","journal-title":"Trends Biotechnol"},{"key":"10.1016\/j.nbt.2019.01.002_bib0145","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1111\/j.1462-2920.2007.01272.x","article-title":"Escherichia coli has multiple enzymes that attack TNT and release nitrogen for growth","volume":"9","author":"Gonz\u00e1lez-P\u00e9rez","year":"2007","journal-title":"Environ Microbiol"},{"key":"10.1016\/j.nbt.2019.01.002_bib0150","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1080\/10242420500090169","article-title":"Biodegradation of 2,4,6-trinitrotoluene (TNT): an enzymatic perspective","volume":"23","author":"Nyanhongo","year":"2005","journal-title":"Biocatal Biotransformation"},{"key":"10.1016\/j.nbt.2019.01.002_bib0155","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0168-1656(01)00422-9","article-title":"Xenobiotics in the environment: present and future strategies to obviate the problem of biological persistence","volume":"94","author":"Rieger","year":"2002","journal-title":"J Biotechnol"},{"key":"10.1016\/j.nbt.2019.01.002_bib0160","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1006\/jmbi.2001.4653","article-title":"The structure of Escherichia coli nitroreductase complexed with nicotinic acid: three crystal forms at 1.7 A, 1.8 A and 2.4 A resolution","volume":"309","author":"Lovering","year":"2001","journal-title":"J Mol Biol"}],"container-title":["New Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S187167841830195X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S187167841830195X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T10:01:53Z","timestamp":1761732113000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S187167841830195X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5]]},"references-count":32,"alternative-id":["S187167841830195X"],"URL":"https:\/\/doi.org\/10.1016\/j.nbt.2019.01.002","relation":{},"ISSN":["1871-6784"],"issn-type":[{"value":"1871-6784","type":"print"}],"subject":[],"published":{"date-parts":[[2019,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Overexpression of the nitroreductase NfsB in an E. coli strain as a whole-cell biocatalyst for the production of chlorinated analogues of the natural herbicide DIBOA","name":"articletitle","label":"Article Title"},{"value":"New Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.nbt.2019.01.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}