{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:59:37Z","timestamp":1791302377227,"version":"4.1.0"},"reference-count":86,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["331065168"],"award-info":[{"award-number":["331065168"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["New Biotechnology"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.nbt.2026.08.002","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T15:14:36Z","timestamp":1785942876000},"page":"51-64","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["An integrated automated pipeline for cloning, transient expression, and purification augmenting the plant cell pack platform for biopharmaceutical screening"],"prefix":"10.1016","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-9446-3912","authenticated-orcid":false,"given":"Monique","family":"Schulze","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Opdensteinen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandor","family":"Albert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niklas","family":"Gabriel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Csaba Matyas","family":"Demeter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Buyel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Nausch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.nbt.2026.08.002_bib1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1208\/s12249-024-02802-0","article-title":"A comprehensive analysis of biopharmaceutical products listed in the FDA's Purple Book","volume":"25","author":"Chen","year":"2024","journal-title":"AAPS PharmSciTech"},{"key":"10.1016\/j.nbt.2026.08.002_bib2","doi-asserted-by":"crossref","DOI":"10.37349\/eds.2025.100880","article-title":"What are biopharmaceuticals?","author":"Pande","year":"2025","journal-title":"Explor Drug Sci"},{"issue":"1","key":"10.1016\/j.nbt.2026.08.002_bib3","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/cbdd.12055","article-title":"The future of peptide-based drugs","volume":"81","author":"Craik","year":"2013","journal-title":"Chem Biol Drug Des"},{"issue":"8","key":"10.1016\/j.nbt.2026.08.002_bib4","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202409604","article-title":"Defining structure\u2013function relationships of amphiphilic excipients enables rational design of ultra-stable biopharmaceuticals","volume":"12","author":"Prossnitz","year":"2025","journal-title":"Adv Sci"},{"issue":"7976","key":"10.1016\/j.nbt.2026.08.002_bib5","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1038\/s41586-023-06415-8","article-title":"De novo design of protein structure and function with RFdiffusion","volume":"620","author":"Watson","year":"2023","journal-title":"Nature"},{"issue":"6615","key":"10.1016\/j.nbt.2026.08.002_bib6","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1126\/science.add2187","article-title":"Robust deep learning\u2013based protein sequence design using ProteinMPNN","volume":"378","author":"Dauparas","year":"2022","journal-title":"Science"},{"issue":"19","key":"10.1016\/j.nbt.2026.08.002_bib7","article-title":"Boltz-1: democratizing biomolecular interaction modeling","volume":"11","author":"Wohlwend","year":"2024","journal-title":"bioRxiv"},{"issue":"10","key":"10.1016\/j.nbt.2026.08.002_bib8","article-title":"Chai-1: decoding the molecular interactions of life","volume":"10","author":"Chai Discovery Team","year":"2024","journal-title":"bioRxiv"},{"issue":"1","key":"10.1016\/j.nbt.2026.08.002_bib9","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1038\/s42003-024-07113-5","article-title":"The Nobel Prize in Chemistry: past, present, and future of AI in biology","volume":"7","author":"Abriata","year":"2024","journal-title":"Commun Biol"},{"issue":"8016","key":"10.1016\/j.nbt.2026.08.002_bib10","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.nbt.2026.08.002_bib11","doi-asserted-by":"crossref","DOI":"10.3389\/fbinf.2025.1533983","article-title":"Unlocking the potential of in silico approaches in designing antibodies against SARS-CoV-2","volume":"5","author":"Subramaniam","year":"2025","journal-title":"Front Bioinform"},{"key":"10.1016\/j.nbt.2026.08.002_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.addr.2022.114143","article-title":"Tools for computational design and high-throughput screening of therapeutic enzymes","volume":"183","author":"Vasina","year":"2022","journal-title":"Adv Drug Deliv Rev"},{"key":"10.1016\/j.nbt.2026.08.002_bib13","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/978-1-60327-058-8_10","article-title":"Automated recombinant protein expression screening in Escherichia coli","volume":"426","author":"Busso","year":"2008","journal-title":"Methods Mol Biol"},{"issue":"1","key":"10.1016\/j.nbt.2026.08.002_bib14","doi-asserted-by":"crossref","first-page":"2697","DOI":"10.1038\/s41467-022-30355-y","article-title":"PlasmidMaker is a versatile, automated, and high-throughput end-to-end platform for plasmid construction","volume":"13","author":"Enghiad","year":"2022","journal-title":"Nat Commun"},{"issue":"12","key":"10.1016\/j.nbt.2026.08.002_bib15","doi-asserted-by":"crossref","first-page":"2624","DOI":"10.1002\/bit.25663","article-title":"Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using Pichia pastoris","volume":"112","author":"Shah","year":"2015","journal-title":"Biotechnol Bioeng"},{"key":"10.1016\/j.nbt.2026.08.002_bib16","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/978-1-4939-9024-5_7","article-title":"High-throughput screening and selection of Pichia pastoris strains","volume":"1923","author":"Weis","year":"2019","journal-title":"Methods Mol Biol"},{"issue":"6","key":"10.1016\/j.nbt.2026.08.002_bib17","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1002\/btpr.2721","article-title":"High-throughput screening of antibody-expressing CHO clones using an automated shaken deep-well system","volume":"34","author":"Wang","year":"2018","journal-title":"Biotechnol Prog"},{"issue":"1","key":"10.1016\/j.nbt.2026.08.002_bib18","doi-asserted-by":"crossref","DOI":"10.1080\/19420862.2021.1955433","article-title":"An end-to-end automated platform process for high-throughput engineering of next-generation multi-specific antibody therapeutics","volume":"13","author":"Furtmann","year":"2021","journal-title":"MAbs"},{"issue":"9","key":"10.1016\/j.nbt.2026.08.002_bib19","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.1002\/bit.25601","article-title":"Optimization and automation of an end-to-end high-throughput microscale transient protein production process","volume":"112","author":"Bos","year":"2015","journal-title":"Biotechnol Bioeng"},{"key":"10.1016\/j.nbt.2026.08.002_bib20","series-title":"Recombinant protein expression in mammalian cells: methods and protocols","first-page":"179","article-title":"High-throughput transfection of HEK293 cells for transient protein production","author":"Arena","year":"2018"},{"key":"10.1016\/j.nbt.2026.08.002_bib21","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/978-1-4939-2205-5_11","article-title":"Transient expression in HEK293 cells: an alternative to Escherichia coli for the production of secreted and intracellular mammalian proteins","volume":"1258","author":"Nettleship","year":"2015","journal-title":"Methods Mol Biol"},{"issue":"3","key":"10.1016\/j.nbt.2026.08.002_bib22","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1007\/s00253-011-3758-5","article-title":"CHO cells in biotechnology for production of recombinant proteins: current state and further potential","volume":"93","author":"Kim","year":"2012","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.nbt.2026.08.002_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2024.108403","article-title":"Towards a seamless product and process development workflow for recombinant proteins produced by plant molecular farming","volume":"75","author":"Buyel","year":"2024","journal-title":"Biotechnol Adv"},{"key":"10.1016\/j.nbt.2026.08.002_bib24","series-title":"Biopharmaceutical manufacturing: progress, trends and challenges","first-page":"241","article-title":"Production of complex proteins in plants: from farming to manufacturing","author":"Nausch","year":"2023"},{"key":"10.1016\/j.nbt.2026.08.002_bib25","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1146\/annurev-genet-120215-035320","article-title":"Integration of Agrobacterium T-DNA into the plant genome","volume":"51","author":"Gelvin","year":"2017","journal-title":"Annu Rev Genet"},{"issue":"16","key":"10.1016\/j.nbt.2026.08.002_bib26","doi-asserted-by":"crossref","first-page":"6297","DOI":"10.1073\/pnas.0304346101","article-title":"A natural variant of a host RNA-dependent RNA polymerase is associated with increased susceptibility to viruses by Nicotiana benthamiana","volume":"101","author":"Yang","year":"2004","journal-title":"Proc Natl Acad Sci USA"},{"issue":"8","key":"10.1016\/j.nbt.2026.08.002_bib27","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1080\/19420862.2016.1227901","article-title":"Techno-economic analysis of a transient plant-based platform for monoclonal antibody production","volume":"8","author":"Nandi","year":"2016","journal-title":"MAbs"},{"issue":"12","key":"10.1016\/j.nbt.2026.08.002_bib28","doi-asserted-by":"crossref","first-page":"2420","DOI":"10.1093\/annonc\/mdq256","article-title":"Rapid, high-yield production in plants of individualized idiotype vaccines for non-Hodgkin's lymphoma","volume":"21","author":"Bendandi","year":"2010","journal-title":"Ann Oncol"},{"issue":"4","key":"10.1016\/j.nbt.2026.08.002_bib29","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1104\/pp.114.250720","article-title":"Proteolytic and N-glycan processing of human \u03b11-antitrypsin expressed in Nicotiana benthamiana","volume":"166","author":"Castilho","year":"2014","journal-title":"Plant Physiol"},{"issue":"5","key":"10.1016\/j.nbt.2026.08.002_bib30","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1111\/j.1467-7652.2009.00496.x","article-title":"The production of hemagglutinin-based virus-like particles in plants: a rapid, efficient and safe response to pandemic influenza","volume":"8","author":"D'Aoust","year":"2010","journal-title":"Plant Biotechnol J"},{"issue":"10","key":"10.1016\/j.nbt.2026.08.002_bib31","first-page":"567","article-title":"Tobacco against Ebola virus disease","volume":"72","author":"Budzianowski","year":"2015","journal-title":"Przegl Lek"},{"issue":"22","key":"10.1016\/j.nbt.2026.08.002_bib32","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1056\/NEJMoa2201300","article-title":"Efficacy and safety of a recombinant plant-based adjuvanted Covid-19 vaccine","volume":"386","author":"Hager","year":"2022","journal-title":"N Engl J Med"},{"issue":"2","key":"10.1016\/j.nbt.2026.08.002_bib33","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.biotechadv.2011.08.007","article-title":"GMP issues for recombinant plant-derived pharmaceutical proteins","volume":"30","author":"Fischer","year":"2012","journal-title":"Biotechnol Adv"},{"issue":"3","key":"10.1016\/j.nbt.2026.08.002_bib34","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1021\/bp990042v","article-title":"Design of mixing systems for plant cell suspensions in stirred reactors","volume":"15","author":"Doran","year":"1999","journal-title":"Biotechnol Prog"},{"issue":"3","key":"10.1016\/j.nbt.2026.08.002_bib35","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.bej.2009.02.008","article-title":"Bioreactor engineering for recombinant protein production in plant cell suspension cultures","volume":"45","author":"Huang","year":"2009","journal-title":"Biochem Eng J"},{"key":"10.1016\/j.nbt.2026.08.002_bib36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0074-7696(08)62452-3","article-title":"Tobacco BY-2 cell line as the \u201cHeLa\u201d cell in the cell biology of higher plants","volume":"132","author":"Nagata","year":"1992","journal-title":"Int Rev Cytol"},{"key":"10.1016\/j.nbt.2026.08.002_bib37","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3389\/fpls.2018.00045","article-title":"Tobacco BY-2 media component optimization for a cost-efficient recombinant protein production","volume":"9","author":"H\u00e4kkinen","year":"2018","journal-title":"Front Plant Sci"},{"key":"10.1016\/j.nbt.2026.08.002_bib38","doi-asserted-by":"crossref","first-page":"720","DOI":"10.3389\/fpls.2019.00720","article-title":"Critical analysis of the commercial potential of plants for the production of recombinant proteins","volume":"10","author":"Schillberg","year":"2019","journal-title":"Front Plant Sci"},{"key":"10.1016\/j.nbt.2026.08.002_bib39","doi-asserted-by":"crossref","first-page":"297","DOI":"10.3389\/fpls.2016.00297","article-title":"Putting the spotlight back on plant suspension cultures","volume":"7","author":"Santos","year":"2016","journal-title":"Front Plant Sci"},{"key":"10.1016\/j.nbt.2026.08.002_bib40","first-page":"S20","article-title":"Investigation of the metabolism of Nicotiana tabacum cells using the respiratory monitoring system (RAMOS)","volume":"45","author":"Da Ullisch","year":"2009","journal-title":"Vitr Cell Dev Biol Anim"},{"key":"10.1016\/j.nbt.2026.08.002_bib41","article-title":"Quantitative transient protein expression in plant tissue culture","author":"Curtis","year":"2000","journal-title":"US Pat US6740526B1"},{"key":"10.1016\/j.nbt.2026.08.002_bib42","article-title":"Method for the generation and cultivation of a plant cell pack","author":"Rademacher","year":"2013","journal-title":"WO Pat WO2013113504A1"},{"issue":"8","key":"10.1016\/j.nbt.2026.08.002_bib43","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1111\/pbi.13081","article-title":"Plant cell packs: a scalable platform for recombinant protein production and metabolic engineering","volume":"17","author":"Rademacher","year":"2019","journal-title":"Plant Biotechnol J"},{"key":"10.1016\/j.nbt.2026.08.002_bib44","doi-asserted-by":"crossref","first-page":"393","DOI":"10.3389\/fbioe.2020.00393","article-title":"Robot cookies\u2014plant cell packs as an automated high-throughput screening platform based on transient expression","volume":"8","author":"Gengenbach","year":"2020","journal-title":"Front Bioeng Biotechnol"},{"issue":"1","key":"10.1016\/j.nbt.2026.08.002_bib45","doi-asserted-by":"crossref","DOI":"10.1080\/21655979.2023.2244235","article-title":"Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia","volume":"14","author":"Kn\u00f6dler","year":"2023","journal-title":"Bioengineered"},{"issue":"9","key":"10.1016\/j.nbt.2026.08.002_bib46","doi-asserted-by":"crossref","first-page":"2236","DOI":"10.1002\/bit.27076","article-title":"Comparison of microbial and transient expression (tobacco plants and plant-cell packs) for the production and purification of the anticancer mistletoe lectin viscumin","volume":"116","author":"Gengenbach","year":"2019","journal-title":"Biotechnol Bioeng"},{"issue":"1\u20132","key":"10.1016\/j.nbt.2026.08.002_bib47","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s11103-013-0049-9","article-title":"Plant species and organ influence the structure and subcellular localization of recombinant glycoproteins","volume":"83","author":"Arcalis","year":"2013","journal-title":"Plant Mol Biol"},{"issue":"7","key":"10.1016\/j.nbt.2026.08.002_bib48","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1111\/j.1467-7652.2008.00354.x","article-title":"Considerations for extraction of monoclonal antibodies targeted to different subcellular compartments in transgenic tobacco plants","volume":"6","author":"Hassan","year":"2008","journal-title":"Plant Biotechnol J"},{"issue":"2","key":"10.1016\/j.nbt.2026.08.002_bib49","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1002\/bit.24715","article-title":"Predictive models for the accumulation of a fluorescent marker protein in tobacco leaves according to the promoter\/5\u2032UTR combination","volume":"110","author":"Buyel","year":"2013","journal-title":"Biotechnol Bioeng"},{"issue":"2","key":"10.1016\/j.nbt.2026.08.002_bib50","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.biotechadv.2009.11.008","article-title":"Tobacco, a highly efficient green bioreactor for production of therapeutic proteins","volume":"28","author":"Tremblay","year":"2010","journal-title":"Biotechnol Adv"},{"issue":"3","key":"10.1016\/j.nbt.2026.08.002_bib51","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.biotechadv.2011.01.002","article-title":"Towards high-yield production of pharmaceutical proteins with plant cell suspension cultures","volume":"29","author":"Xu","year":"2011","journal-title":"Biotechnol Adv"},{"issue":"1\u20132","key":"10.1016\/j.nbt.2026.08.002_bib52","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s11103-018-0726-9","article-title":"Expression of VGRNb-PE immunotoxin in transplastomic lettuce (Lactuca sativa L.)","volume":"97","author":"Mirzaee","year":"2018","journal-title":"Plant Mol Biol"},{"key":"10.1016\/j.nbt.2026.08.002_bib53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jbiotec.2024.05.003","article-title":"Optimizing interleukin-6 and -8 expression, clarification and purification in plant cell packs and plants for application in advanced therapy medicinal products and cellular agriculture","volume":"390","author":"Opdensteinen","year":"2024","journal-title":"J Biotechnol"},{"issue":"5","key":"10.1016\/j.nbt.2026.08.002_bib54","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1007\/s00425-015-2377-2","article-title":"Synthetic promoters in planta","volume":"242","author":"Dey","year":"2015","journal-title":"Planta"},{"issue":"Pt 5","key":"10.1016\/j.nbt.2026.08.002_bib55","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1099\/vir.0.82718-0","article-title":"Optimization of human papillomavirus type 16 L1 expression in plants","volume":"88","author":"Maclean","year":"2007","journal-title":"J Gen Virol"},{"issue":"2","key":"10.1016\/j.nbt.2026.08.002_bib56","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/j.1467-7652.2007.00306.x","article-title":"Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1","volume":"6","author":"Rademacher","year":"2008","journal-title":"Plant Biotechnol J"},{"key":"10.1016\/j.nbt.2026.08.002_bib57","unstructured":"Gengenbach B.B. Rational and model-based characterization of heterologous gene expression in biological systems [dissertation]. Aachen: RWTH Aachen University; 2021."},{"key":"10.1016\/j.nbt.2026.08.002_bib58","unstructured":"Opdensteinen P. Assessment of a novel high-throughput process development platform for biopharmaceutical protein production [dissertation]. Aachen: RWTH Aachen University; 2023."},{"issue":"1","key":"10.1016\/j.nbt.2026.08.002_bib59","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1093\/oxfordjournals.pcp.a028913","article-title":"Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants","volume":"37","author":"Mitsuhara","year":"1996","journal-title":"Plant Cell Physiol"},{"issue":"9","key":"10.1016\/j.nbt.2026.08.002_bib60","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.1073\/pnas.80.9.2591","article-title":"UV-induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense","volume":"80","author":"Kreuzaler","year":"1983","journal-title":"Proc Natl Acad Sci USA"},{"issue":"8","key":"10.1016\/j.nbt.2026.08.002_bib61","doi-asserted-by":"crossref","first-page":"3257","DOI":"10.1093\/nar\/15.8.3257","article-title":"The 5\u2032 leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo","volume":"15","author":"Gallie","year":"1987","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"10.1016\/j.nbt.2026.08.002_bib62","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1128\/jvi.64.4.1590-1597.1990","article-title":"Cap-independent enhancement of translation by a plant potyvirus 5\u2032 nontranslated region","volume":"64","author":"Carrington","year":"1990","journal-title":"J Virol"},{"key":"10.1016\/j.nbt.2026.08.002_bib63","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.pep.2018.06.003","article-title":"Expression and purification of human phosphatase and actin regulator 1 (PHACTR1) in plant-based systems","volume":"151","author":"Gengenbach","year":"2018","journal-title":"Protein Expr Purif"},{"issue":"3","key":"10.1016\/j.nbt.2026.08.002_bib64","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1073\/pnas.85.3.846","article-title":"rbcS genes in Solanum tuberosum: conservation of transit peptide and exon shuffling during evolution","volume":"85","author":"Wolter","year":"1988","journal-title":"Proc Natl Acad Sci USA"},{"issue":"1","key":"10.1016\/j.nbt.2026.08.002_bib65","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/BF01682088","article-title":"Reduced virus infectivity in Nicotiana tabacum secreting a TMV-specific full-size antibody","volume":"1","author":"Voss","year":"1995","journal-title":"Mol Breed"},{"issue":"5","key":"10.1016\/j.nbt.2026.08.002_bib66","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1016\/0092-8674(87)90086-9","article-title":"A C-terminal signal prevents secretion of luminal ER proteins","volume":"48","author":"Munro","year":"1987","journal-title":"Cell"},{"issue":"22","key":"10.1016\/j.nbt.2026.08.002_bib67","doi-asserted-by":"crossref","first-page":"10362","DOI":"10.1073\/pnas.88.22.10362","article-title":"A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole","volume":"88","author":"Neuhaus","year":"1991","journal-title":"Proc Natl Acad Sci USA"},{"issue":"3","key":"10.1016\/j.nbt.2026.08.002_bib68","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s11693-010-9062-3","article-title":"The GeneOptimizer algorithm: using a sliding window approach to cope with the vast sequence space in multiparameter DNA sequence optimization","volume":"4","author":"Raab","year":"2010","journal-title":"Syst Synth Biol"},{"issue":"1","key":"10.1016\/j.nbt.2026.08.002_bib69","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.seppur.2009.04.016","article-title":"Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt","volume":"68","author":"Luechau","year":"2009","journal-title":"Sep Purif Technol"},{"issue":"4","key":"10.1016\/j.nbt.2026.08.002_bib70","doi-asserted-by":"crossref","DOI":"10.1002\/biot.201600721","article-title":"Animal component-free Agrobacterium tumefaciens cultivation media for better GMP compliance increases biomass yield and pharmaceutical protein expression in Nicotiana benthamiana","volume":"12","author":"Houdelet","year":"2017","journal-title":"Biotechnol J"},{"issue":"3","key":"10.1016\/j.nbt.2026.08.002_bib71","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1007\/BF00331014","article-title":"The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector","volume":"204","author":"Koncz","year":"1986","journal-title":"Mol Gen Genet"},{"key":"10.1016\/j.nbt.2026.08.002_bib72","first-page":"405","article-title":"Electroporation protocols for Agrobacterium","volume":"44","author":"Main","year":"1995","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.nbt.2026.08.002_bib73","series-title":"Agrobacterium protocols","first-page":"43","article-title":"Three methods for the introduction of foreign DNA into Agrobacterium","author":"Wise","year":"2006"},{"issue":"4","key":"10.1016\/j.nbt.2026.08.002_bib74","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1002\/btpr.2290","article-title":"Statistical analysis of data from limiting dilution cloning to assess monoclonality in generating manufacturing cell lines","volume":"32","author":"Quiroz","year":"2016","journal-title":"Biotechnol Prog"},{"key":"10.1016\/j.nbt.2026.08.002_bib75","doi-asserted-by":"crossref","DOI":"10.3389\/fceng.2021.737010","article-title":"Nicotiana spp. for the expression and purification of functional IgG3 antibodies directed against the Staphylococcus aureus alpha toxin","volume":"3","author":"Opdensteinen","year":"2021","journal-title":"Front Chem Eng"},{"issue":"6","key":"10.1016\/j.nbt.2026.08.002_bib76","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1021\/bc00030a023","article-title":"Preparation and characterization of a bifunctional fusion enzyme composed of UDP-galactose 4-epimerase and galactose-1-P uridylyltransferase","volume":"5","author":"Tamada","year":"1994","journal-title":"Bioconjug Chem"},{"issue":"1","key":"10.1016\/j.nbt.2026.08.002_bib77","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jbiotec.2008.07.1816","article-title":"A high-throughput and single-tube recombination of crude PCR products using a DNA polymerase inhibitor and type IIS restriction enzyme","volume":"137","author":"Kotera","year":"2008","journal-title":"J Biotechnol"},{"issue":"4","key":"10.1016\/j.nbt.2026.08.002_bib78","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1002\/bit.25836","article-title":"Enhanced purification of plasmid DNA isoforms by exploiting ionic strength effects during ultrafiltration","volume":"113","author":"Li","year":"2016","journal-title":"Biotechnol Bioeng"},{"issue":"1","key":"10.1016\/j.nbt.2026.08.002_bib79","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.biotechadv.2014.11.005","article-title":"Synthetic fusion protein design and applications","volume":"33","author":"Yu","year":"2015","journal-title":"Biotechnol Adv"},{"issue":"5","key":"10.1016\/j.nbt.2026.08.002_bib80","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1021\/acssynbio.4c00006","article-title":"Synthetic genomics: repurposing biological systems for applications in engineering biology","volume":"13","author":"Fu","year":"2024","journal-title":"ACS Synth Biol"},{"issue":"2","key":"10.1016\/j.nbt.2026.08.002_bib81","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/BF00267408","article-title":"Transfection and transformation of Agrobacterium tumefaciens","volume":"163","author":"Holsters","year":"1978","journal-title":"Mol Gen Genet"},{"key":"10.1016\/j.nbt.2026.08.002_bib82","series-title":"Cancer cell culture: methods and protocols","first-page":"93","article-title":"Isolation and culture of melanoma cell lines","author":"Riker","year":"2004"},{"key":"10.1016\/j.nbt.2026.08.002_bib83","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/0076-6879(86)21039-3","article-title":"Poisson statistical analysis of repetitive subcloning by the limiting dilution technique as a way of assessing hybridoma monoclonality","volume":"121","author":"Coller","year":"1986","journal-title":"Methods Enzymol"},{"issue":"191","key":"10.1016\/j.nbt.2026.08.002_bib84","doi-asserted-by":"crossref","DOI":"10.3791\/64298","article-title":"Fast colony forming unit counting in 96-well plate format applied to the Drosophila microbiome","author":"Dodge","year":"2023","journal-title":"J Vis Exp"},{"issue":"7","key":"10.1016\/j.nbt.2026.08.002_bib85","doi-asserted-by":"crossref","first-page":"2227","DOI":"10.1021\/acssynbio.4c00298","article-title":"AutoBioTech\u2014a versatile biofoundry for automated strain engineering","volume":"13","author":"Rosch","year":"2024","journal-title":"ACS Synth Biol"},{"key":"10.1016\/j.nbt.2026.08.002_bib86","unstructured":"Singer Instruments. ROTOR+ PIXL sales brochure. Watchet (UK): Singer Instruments; 2021. Available from: https:\/\/www.singerinstruments.com\/wp-content\/uploads\/2022\/03\/ROTOR-PIXL-Sales-brochure-2021_Digital_4mb.pdf."}],"container-title":["New Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1871678426000981?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1871678426000981?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:37:53Z","timestamp":1791301073000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1871678426000981"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":86,"alternative-id":["S1871678426000981"],"URL":"https:\/\/doi.org\/10.1016\/j.nbt.2026.08.002","relation":{},"ISSN":["1871-6784"],"issn-type":[{"value":"1871-6784","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An integrated automated pipeline for cloning, transient expression, and purification augmenting the plant cell pack platform for biopharmaceutical screening","name":"articletitle","label":"Article Title"},{"value":"New Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.nbt.2026.08.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}