{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:58:30Z","timestamp":1787169510673,"version":"build-2736575974"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Neuroscience Letters"],"published-print":{"date-parts":[[2025,1]]},"DOI":"10.1016\/j.neulet.2024.138038","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T12:19:46Z","timestamp":1731413986000},"page":"138038","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Optimized primary dorsal root ganglion cell culture protocol for reliable K+ current patch-clamp recordings"],"prefix":"10.1016","volume":"844","author":[{"given":"Feyza Alyu","family":"Altinok","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilhem","family":"Dallali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abderaouf","family":"Boubekka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Hasan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf","family":"Ozturk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.neulet.2024.138038_b0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.ynpai.2021.100063","article-title":"Cellular models of pain: new technologies and their potential to progress preclinical research","volume":"10","author":"Chrysostomidou","year":"2021","journal-title":"Neurobiol. Pain."},{"issue":"12","key":"10.1016\/j.neulet.2024.138038_b0010","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0168228","article-title":"Primary postnatal dorsal root ganglion culture from conventionally slaughtered calves","volume":"11","author":"Fadda","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.neulet.2024.138038_b0015","doi-asserted-by":"crossref","DOI":"10.1177\/17448069231197102","article-title":"Compartmentalized primary cultures of dorsal root ganglion neurons to model peripheral pathophysiological conditions","volume":"19","author":"Giorgi","year":"2023","journal-title":"Mol. Pain."},{"key":"10.1016\/j.neulet.2024.138038_b0020","first-page":"e57569","article-title":"Dorsal root ganglia isolation and primary culture to study neurotransmitter release","volume":"140","author":"Lin","year":"2018","journal-title":"J. vis. Exp."},{"key":"10.1016\/j.neulet.2024.138038_b0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.jneumeth.2024.110143","article-title":"Electrophysiological properties of dorsal root ganglion neurons cultured on 3D silicon micro-pillar substrates","volume":"407","author":"Marciu\u0161","year":"2024","journal-title":"J. Neurosci. Methods."},{"issue":"10","key":"10.1016\/j.neulet.2024.138038_b0030","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1517\/17460440903266829","article-title":"Dorsal root ganglia sensory neuronal cultures: a tool for drug discovery for peripheral neuropathies","volume":"4","author":"Melli","year":"2009","journal-title":"Expert Opin. Drug Discov."},{"issue":"7","key":"10.1016\/j.neulet.2024.138038_b0035","doi-asserted-by":"crossref","first-page":"3591","DOI":"10.3390\/ijms25073591","article-title":"Mechanisms of action of dorsal root ganglion stimulation","volume":"25","author":"Abd-Elsayed","year":"2024","journal-title":"Int. J. Mol. Sci."},{"issue":"6242","key":"10.1016\/j.neulet.2024.138038_b0040","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.1126\/science.aab2374","article-title":"Promoting an open research culture","volume":"348","author":"Nosek","year":"2015","journal-title":"Science"},{"issue":"2","key":"10.1016\/j.neulet.2024.138038_b0045","first-page":"101","article-title":"Antibiotic supplements affect electrophysiological properties and excitability of rat hippocampal pyramidal neurons in primary culture","volume":"17","author":"Bahrami","year":"2013","journal-title":"Iranian Biomed. J."},{"issue":"1","key":"10.1016\/j.neulet.2024.138038_b0050","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1146\/annurev.bi.49.070180.005215","article-title":"Collagenases","volume":"49","author":"Harper","year":"1980","journal-title":"Annu. Rev. Biochem."},{"issue":"1","key":"10.1016\/j.neulet.2024.138038_b0055","doi-asserted-by":"crossref","first-page":"5713","DOI":"10.1038\/s41598-022-09605-y","article-title":"Different methods of detaching adherent cells and their effects on the cell surface expression of Fas receptor and Fas ligand","volume":"12","author":"Lai","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.neulet.2024.138038_b0060","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.yjmcc.2021.08.008","article-title":"Bottom-up proteomic analysis of human adult cardiac tissue and isolated cardiomyocytes","volume":"162","author":"Wojtkiewicz","year":"2022","journal-title":"J. Mol. Cell. Cardiol."},{"issue":"2","key":"10.1016\/j.neulet.2024.138038_b0065","doi-asserted-by":"crossref","first-page":"e1063","DOI":"10.1002\/term.2432","article-title":"Control of osmotic pressure to improve cell viability in cell\u2010laden tissue engineering constructs","volume":"12","author":"Carvalho","year":"2018","journal-title":"J. Tissue Eng. Reg. Med."},{"issue":"4","key":"10.1016\/j.neulet.2024.138038_b0070","first-page":"95","article-title":"Evaluation of PBS treatment and PEI coating effects on surface morphology and cellular response of 3D-printed alginate scaffolds","volume":"8","author":"Garc\u00eda","year":"2017","journal-title":"J. Funct. Biomater."},{"issue":"1","key":"10.1016\/j.neulet.2024.138038_b0075","doi-asserted-by":"crossref","DOI":"10.1002\/9780471729259.mca04es11","article-title":"Growth and maintenance of Vero cell lines","volume":"11","author":"Ammerman","year":"2008","journal-title":"Curr. Protoc. Microbiol."},{"issue":"4","key":"10.1016\/j.neulet.2024.138038_b0080","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1111\/j.1745-7254.2008.00777.x","article-title":"Electrophysiological characterization of a novel Kv channel blocker N,N\u2032\u2010[oxybis(2,1\u2010ethanediyloxy\u20102,1\u2010ethanediyl)] bis(4\u2010methyl)\u2010benzenesulfonamide found in virtual screening","volume":"29","author":"Gao","year":"2008","journal-title":"Acta Pharm. Sinica"},{"issue":"2","key":"10.1016\/j.neulet.2024.138038_b0085","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2021.100439","article-title":"Combining whole-cell patch clamp and dye loading in acute brain slices with bulk RNA sequencing in embryonic to aged mice","volume":"2","author":"Kamen","year":"2021","journal-title":"STAR Protoc."},{"issue":"1","key":"10.1016\/j.neulet.2024.138038_b0090","first-page":"71","article-title":"Electrophoretic behavior of charge regulated zwitter ionic buffers in covalently and dynamically coated fused silica capillaries","volume":"52","author":"Al-Ghobashy","year":"2014","journal-title":"Bull. Fac. Pharm. Cairo Univ."},{"key":"10.1016\/j.neulet.2024.138038_b0095","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4939-9077-1_9","article-title":"Whole-cell patch-clamp electrophysiology to study ionotropic glutamatergic receptors and their roles in addiction","volume":"107\u2013135","author":"Leyrer-Jackson","year":"2019","journal-title":"Glutamate Receptors: Methods and Protocols"},{"issue":"1","key":"10.1016\/j.neulet.2024.138038_b0100","article-title":"Cellular stress responses: cell survival and cell death","volume":"2010","author":"Fulda","year":"2010","journal-title":"Int. J. Cell Biol."},{"issue":"11","key":"10.1016\/j.neulet.2024.138038_b0105","doi-asserted-by":"crossref","first-page":"3893","DOI":"10.1529\/biophysj.106.099119","article-title":"Ionic requirements for membrane-glass adhesion and gigaseal formation in patch-clamp recording","volume":"92","author":"Priel","year":"2007","journal-title":"Biophys. J."},{"issue":"1","key":"10.1016\/j.neulet.2024.138038_b0110","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1111\/jphp.12496","article-title":"In vivo wound healing activity of gels containing Cecropia pachystachya leaves","volume":"68","author":"Duque","year":"2016","journal-title":"J. Pharm. Pharmacol."},{"key":"10.1016\/j.neulet.2024.138038_b0115","doi-asserted-by":"crossref","unstructured":"A. Molleman, Whole-cell protocols and data analysis. in: patch clamping an introd. guid. to patch clamp electrophysiol., John Wiley & Sons, Ltd, Chichester, UK, 2003, pp. 115-139.","DOI":"10.1002\/0470856521.ch5"},{"issue":"3","key":"10.1016\/j.neulet.2024.138038_b0120","doi-asserted-by":"crossref","first-page":"156","DOI":"10.3109\/10799893.2012.672993","article-title":"Modulation of action potential and calcium signaling by levetiracetam in rat sensory neurons","volume":"32","author":"Ozcan","year":"2012","journal-title":"J. Recep. Signal Transduc."},{"issue":"2","key":"10.1016\/j.neulet.2024.138038_b0125","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/S0006-8993(01)02915-8","article-title":"Activation of melatonin receptor increases a delayed rectifier K+ current in rat cerebellar granule cells","volume":"917","author":"Huan","year":"2001","journal-title":"Brain Res."},{"issue":"1\u20132","key":"10.1016\/j.neulet.2024.138038_b0130","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0006-8993(02)02549-0","article-title":"Evaluation of \u03b2-amyloid peptide 25\u201335 on calcium homeostasis in cultured rat dorsal root ganglion neurons","volume":"939","author":"He","year":"2002","journal-title":"Brain Res."},{"key":"10.1016\/j.neulet.2024.138038_b0135","series-title":"Encyclopedia of Computational Neuroscience","article-title":"Delayed rectifier and A-type potassium channels","author":"Manis","year":"2015"},{"key":"10.1016\/j.neulet.2024.138038_b0140","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2022.977440","article-title":"Apamin structure and pharmacology revisited","volume":"13","author":"Kuzmenkov","year":"2022","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.neulet.2024.138038_b0145","first-page":"111","article-title":"Extracellular and intracellular magnesium-calcium interactions","volume":"15","author":"Vormann","year":"1993","journal-title":"Magnesium Bull."},{"issue":"4","key":"10.1016\/j.neulet.2024.138038_b0150","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1021\/acsearthspacechem.9b00320","article-title":"Ternary complex formation of phosphate with Ca and Mg ions binding to ferrihydrite: Experiments and mechanisms","volume":"4","author":"Mendez","year":"2020","journal-title":"ACS Earth Space Chem."},{"key":"10.1016\/j.neulet.2024.138038_b0155","series-title":"Calcium chelators and calcium ionophores, In Calcium in Drug Actions","first-page":"491","author":"Bers","year":"1988"}],"container-title":["Neuroscience Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304394024004178?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304394024004178?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T03:14:22Z","timestamp":1732850062000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304394024004178"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":31,"alternative-id":["S0304394024004178"],"URL":"https:\/\/doi.org\/10.1016\/j.neulet.2024.138038","relation":{},"ISSN":["0304-3940"],"issn-type":[{"value":"0304-3940","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Optimized primary dorsal root ganglion cell culture protocol for reliable K+ current patch-clamp recordings","name":"articletitle","label":"Article Title"},{"value":"Neuroscience Letters","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.neulet.2024.138038","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"138038"}}