{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T10:26:37Z","timestamp":1772792797885,"version":"3.50.1"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100018556","name":"Science and Technology Program of Suzhou","doi-asserted-by":"publisher","award":["SKJY2021017"],"award-info":[{"award-number":["SKJY2021017"]}],"id":[{"id":"10.13039\/501100018556","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012154","name":"Graduate Research and Innovation Projects of Jiangsu Province","doi-asserted-by":"publisher","award":["KYCX23_3280"],"award-info":[{"award-number":["KYCX23_3280"]}],"id":[{"id":"10.13039\/501100012154","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013088","name":"Qinglan Project of Jiangsu Province of China","doi-asserted-by":"publisher","award":["2022 NO.29"],"award-info":[{"award-number":["2022 NO.29"]}],"id":[{"id":"10.13039\/501100013088","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82373863"],"award-info":[{"award-number":["82373863"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Neuroscience Letters"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.neulet.2025.138333","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T16:12:25Z","timestamp":1753805545000},"page":"138333","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Delayed administration of rapamycin inhibits glial scar formation through upregulating matrilin-3 in an autophagy-dependent manner in ischemic stroke"],"prefix":"10.1016","volume":"865","author":[{"given":"Yi","family":"Guo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiping","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinzhi","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuping","family":"Shan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tongxin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqi","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigang","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.neulet.2025.138333_b0005","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1177\/17474930211065917","article-title":"World Stroke Organization (WSO): global stroke fact sheet 2022","volume":"17","author":"Feigin","year":"2022","journal-title":"Int. J. Stroke"},{"key":"10.1016\/j.neulet.2025.138333_b0010","doi-asserted-by":"crossref","first-page":"e56","DOI":"10.1161\/CIR.0000000000000659","article-title":"Heart disease and stroke statistics\u20142019 update: a report from the American Heart Association","volume":"139","author":"Benjamin","year":"2019","journal-title":"Circulation"},{"key":"10.1016\/j.neulet.2025.138333_b0015","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.neuroscience.2024.02.017","article-title":"New perspectives in neuroprotection for ischemic stroke","volume":"550","author":"P\u00e9rez-Mato","year":"2024","journal-title":"Neuroscience"},{"key":"10.1016\/j.neulet.2025.138333_b0020","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1038\/s41582-021-00605-6","article-title":"Pharmacological brain cytoprotection in acute ischaemic stroke \u2014 renewed hope in the reperfusion era","volume":"18","author":"Fisher","year":"2022","journal-title":"Nat. Rev. Neurol."},{"key":"10.1016\/j.neulet.2025.138333_b0025","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1038\/nrn3898","article-title":"Astrocyte barriers to neurotoxic inflammation","volume":"16","author":"Sofroniew","year":"2015","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1016\/j.neulet.2025.138333_b0030","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.7150\/thno.48761","article-title":"M2 microglial small extracellular vesicles reduce glial scar formation via the miR-124\/STAT3 pathway after ischemic stroke in mice","volume":"11","author":"Li","year":"2021","journal-title":"Theranostics"},{"key":"10.1016\/j.neulet.2025.138333_b0035","doi-asserted-by":"crossref","first-page":"241","DOI":"10.5607\/en.2016.25.5.241","article-title":"Inflammation after ischemic stroke: the role of leukocytes and glial cells","volume":"25","author":"Kim","year":"2016","journal-title":"Exp. Neurobiol."},{"key":"10.1016\/j.neulet.2025.138333_b0040","article-title":"Inhibition of GSK3\u03b2 and RIP1K attenuates glial scar formation induced by ischemic stroke via reduction of inflammatory cytokine production","volume":"11","author":"Liu","year":"2020","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.neulet.2025.138333_b0045","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41593-017-0033-9","article-title":"The diversity and disparity of the glial scar","volume":"21","author":"Adams","year":"2018","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.neulet.2025.138333_b0050","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1007\/s12031-019-01417-6","article-title":"Glial scar-a promising target for improving outcomes after CNS injury","volume":"70","author":"He","year":"2020","journal-title":"J. Mol. Neurosci."},{"key":"10.1016\/j.neulet.2025.138333_b0055","doi-asserted-by":"crossref","DOI":"10.3390\/ijms22084280","article-title":"Astrocyte activation in neurovascular damage and repair following ischaemic stroke","volume":"22","author":"Patabendige","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.neulet.2025.138333_b0060","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.nbd.2015.05.003","article-title":"Reactive astrocytes and therapeutic potential in focal ischemic stroke","volume":"85","author":"Choudhury","year":"2016","journal-title":"Neurobiol. Dis."},{"key":"10.1016\/j.neulet.2025.138333_b0065","doi-asserted-by":"crossref","DOI":"10.1016\/j.expneurol.2021.113684","article-title":"An updated review of autophagy in ischemic stroke: from mechanisms to therapies","volume":"340","author":"Wang","year":"2021","journal-title":"Exp. Neurol."},{"key":"10.1016\/j.neulet.2025.138333_b0070","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines11030973","article-title":"PPAR\u03b1 inhibits astrocyte inflammation activation by restoring autophagic flux after transient brain ischemia","volume":"11","author":"Luo","year":"2023","journal-title":"Biomedicines"},{"key":"10.1016\/j.neulet.2025.138333_b0075","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1038\/nrd3531","article-title":"Rapamycin passes the torch: a new generation of mTOR inhibitors","volume":"10","author":"Benjamin","year":"2011","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.neulet.2025.138333_b0080","doi-asserted-by":"crossref","DOI":"10.1186\/s40643-022-00554-y","article-title":"Rapamycin golden jubilee and still the miraculous drug: a potent immunosuppressant, antitumor, rejuvenative agent, and potential contributor in COVID-19 treatment","volume":"9","author":"Mohamed","year":"2022","journal-title":"Bioresour. Bioprocess."},{"key":"10.1016\/j.neulet.2025.138333_b0085","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1002\/acn3.507","article-title":"Rapamycin prevents cerebral stroke by modulating apoptosis and autophagy in penumbra in rats","volume":"5","author":"Wu","year":"2018","journal-title":"Ann. Clin. Transl. Neurol."},{"key":"10.1016\/j.neulet.2025.138333_b0090","doi-asserted-by":"crossref","DOI":"10.3390\/ijms24010676","article-title":"Therapeutic effect of rapamycin on TDP-43-related pathogenesis in ischemic stroke","volume":"24","author":"Tsou","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.neulet.2025.138333_b0095","doi-asserted-by":"crossref","DOI":"10.3389\/fnmol.2020.574041","article-title":"Rapamycin preserves neural tissue, promotes schwann cell myelination and reduces glial scar formation after hemi-contusion spinal cord injury in mice","volume":"13","author":"Liu","year":"2021","journal-title":"Front. Mol. Neurosci."},{"key":"10.1016\/j.neulet.2025.138333_b0100","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.neulet.2018.05.002","article-title":"Effects of long-term rapamycin treatment on glial scar formation after cryogenic traumatic brain injury in mice","volume":"678","author":"Fan","year":"2018","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.neulet.2025.138333_b0105","doi-asserted-by":"crossref","DOI":"10.3390\/ijms17040590","article-title":"Matrilin-3 role in cartilage development and osteoarthritis","volume":"17","author":"Muttigi","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.neulet.2025.138333_b0110","article-title":"Mice lacking the matrilin family of extracellular matrix proteins develop mild skeletal abnormalities and are susceptible to age-associated osteoarthritis","volume":"21","author":"Li","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.neulet.2025.138333_b0115","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2024.113980","article-title":"Matrilin-3 supports neuroprotection in ischemic stroke by suppressing astrocyte-mediated neuroinflammation","volume":"43","author":"Zhou","year":"2024","journal-title":"Cell Rep."},{"key":"10.1016\/j.neulet.2025.138333_b0120","article-title":"VEGFD\/VEGFR3 signaling contributes to the dysfunction of the astrocyte IL-3\/microglia IL-3R\u03b1 cross-talk and drives neuroinflammation in mouse ischemic stroke","author":"Wang","year":"2024","journal-title":"Acta Pharmacol. Sin."},{"key":"10.1016\/j.neulet.2025.138333_b0125","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1038\/s41401-023-01069-8","article-title":"RIPK1 inhibition contributes to lysosomal membrane stabilization in ischemic astrocytes via a lysosomal Hsp70.1B-dependent mechanism","volume":"44","author":"Du","year":"2023","journal-title":"Acta Pharmacol. Sin."},{"key":"10.1016\/j.neulet.2025.138333_b0130","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1038\/aps.2013.5","article-title":"Application and interpretation of current autophagy inhibitors and activators","volume":"34","author":"Yang","year":"2013","journal-title":"Acta Pharmacol. Sin."},{"key":"10.1016\/j.neulet.2025.138333_b0135","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1038\/s41467-021-22111-5","article-title":"Molecular basis of V-ATPase inhibition by bafilomycin A1","volume":"12","author":"Wang","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.neulet.2025.138333_b0140","doi-asserted-by":"crossref","first-page":"21545","DOI":"10.1074\/jbc.M109.016568","article-title":"Proteolytic processing causes extensive heterogeneity of tissue matrilin forms","volume":"284","author":"Ehlen","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.neulet.2025.138333_b0145","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1136\/annrheumdis-2011-200557","article-title":"Autophagy activation by rapamycin reduces severity of experimental osteoarthritis","volume":"71","author":"Caram\u00e9s","year":"2012","journal-title":"Ann. Rheum. Dis."},{"key":"10.1016\/j.neulet.2025.138333_b0150","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.neuint.2015.03.001","article-title":"Inhibition of mTOR pathway restrains astrocyte proliferation, migration and production of inflammatory mediators after oxygen\u2013glucose deprivation and reoxygenation","volume":"83\u201384","author":"Li","year":"2015","journal-title":"Neurochem. Int."},{"key":"10.1016\/j.neulet.2025.138333_b0155","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1111\/j.1755-5949.2012.00295.x","article-title":"Excessive autophagy contributes to neuron death in cerebral ischemia","volume":"18","author":"Shi","year":"2012","journal-title":"CNS Neurosci. Ther."},{"key":"10.1016\/j.neulet.2025.138333_b0160","doi-asserted-by":"crossref","DOI":"10.1016\/j.pharmthera.2021.107848","article-title":"Targeting autophagy in ischemic stroke: from molecular mechanisms to clinical therapeutics","volume":"225","author":"Ajoolabady","year":"2021","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.neulet.2025.138333_b0165","doi-asserted-by":"crossref","first-page":"738","DOI":"10.4161\/auto.6.6.12573","article-title":"Autophagy was activated in injured astrocytes and mildly decreased cell survival following glucose and oxygen deprivation and focal cerebral ischemia","volume":"6","author":"Qin","year":"2010","journal-title":"Autophagy"},{"key":"10.1016\/j.neulet.2025.138333_b0170","doi-asserted-by":"crossref","first-page":"e2618","DOI":"10.1038\/cddis.2017.34","article-title":"Inhibition of autophagy blocks cathepsins\u2013tBid\u2013mitochondrial apoptotic signaling pathway via stabilization of lysosomal membrane in ischemic astrocytes","volume":"8","author":"Zhou","year":"2017","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.neulet.2025.138333_b0175","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2024.1360014","article-title":"Role of autophagy in ischemic stroke: insights from animal models and preliminary evidence in the human disease","volume":"12","author":"Stanzione","year":"2024","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.neulet.2025.138333_b0180","doi-asserted-by":"crossref","first-page":"11101","DOI":"10.1074\/jbc.M611588200","article-title":"Identification of an ADAMTS-4 cleavage motif using phage display leads to the development of fluorogenic peptide substrates and reveals matrilin-3 as a novel substrate","volume":"282","author":"Hills","year":"2007","journal-title":"J. Biol. Chem."}],"container-title":["Neuroscience Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304394025002216?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304394025002216?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T08:00:27Z","timestamp":1772784027000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304394025002216"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":36,"alternative-id":["S0304394025002216"],"URL":"https:\/\/doi.org\/10.1016\/j.neulet.2025.138333","relation":{},"ISSN":["0304-3940"],"issn-type":[{"value":"0304-3940","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Delayed administration of rapamycin inhibits glial scar formation through upregulating matrilin-3 in an autophagy-dependent manner in ischemic stroke","name":"articletitle","label":"Article Title"},{"value":"Neuroscience Letters","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.neulet.2025.138333","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"138333"}}