{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T23:02:48Z","timestamp":1762210968439,"version":"3.44.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004263","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007395","name":"Instituto Nacional de Ci\u00eancia e Tecnologia para Excitotoxicidade e Neuroprote\u00e7\u00e3o","doi-asserted-by":"publisher","award":["EN 465671\/2014-4"],"award-info":[{"award-number":["EN 465671\/2014-4"]}],"id":[{"id":"10.13039\/501100007395","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["NeuroToxicology"],"published-print":{"date-parts":[[2020,3]]},"DOI":"10.1016\/j.neuro.2019.12.013","type":"journal-article","created":{"date-parts":[[2019,12,25]],"date-time":"2019-12-25T09:32:35Z","timestamp":1577266355000},"page":"60-70","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Methionine and methionine sulfoxide induces neurochemical and morphological changes in cultured astrocytes: Involvement of Na+, K+-ATPase activity, oxidative status, and cholinergic and purinergic signaling"],"prefix":"10.1016","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2181-3591","authenticated-orcid":false,"given":"Mayara Sandrielly Pereira","family":"Soares","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalia Stark","family":"Pedra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia Pontes","family":"Bona","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita \u00c1vila","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Cardoso","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Hofstatter","family":"Azambuja","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela TS","family":"Wyse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizandra","family":"Braganhol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francieli Moro","family":"Stefanello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roselia Maria","family":"Spanevello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.neuro.2019.12.013_bib0005","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0076-6879(84)05016-3","article-title":"Catalase in vitro","volume":"105","author":"Aebi","year":"1984","journal-title":"Method Enzymol."},{"key":"10.1016\/j.neuro.2019.12.013_bib0010","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0304-3940(01)01636-6","article-title":"Changes in thiol content and expression of glutathione redox system genes in the hippocampus and cerebellum in Alzheimer\u2019s disease","volume":"302","author":"Aksenov","year":"2001","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.neuro.2019.12.013_bib0015","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.neulet.2013.12.030","article-title":"Heterogeneity of reactive astrocytes","volume":"565","author":"Anderson","year":"2014","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.neuro.2019.12.013_bib0020","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1159\/000355835","article-title":"The non-neuronal cholinergic system in health and disease","volume":"92","author":"Beckmann","year":"2013","journal-title":"Pharmacology"},{"key":"10.1016\/j.neuro.2019.12.013_bib0025","doi-asserted-by":"crossref","first-page":"87","DOI":"10.3389\/fnmol.2019.00087","article-title":"Astrocytes do not forfeit their neuroprotective roles after surviving intense oxidative stress","volume":"12","author":"Bhatia","year":"2019","journal-title":"Front. Mol. Neurosci."},{"key":"10.1016\/j.neuro.2019.12.013_bib0030","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.neuro.2019.12.013_bib0035","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1152\/physrev.00043.2006","article-title":"Physiology and pathophysiology of purinergic neurotransmission","volume":"87","author":"Burnstock","year":"2007","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.neuro.2019.12.013_bib0040","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-94-007-4719-7_1","article-title":"Introduction to purinergic signalling in the brain","volume":"986","author":"Burnstock","year":"2013","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.neuro.2019.12.013_bib0045","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1046\/j.1471-4159.2003.01985.x","article-title":"Acetylcholinesterase induces neuronal cell loss, astrocyte hypertrophy and behavioral deficits in mammalian hippocampus","volume":"87","author":"Chac\u00f3n","year":"2003","journal-title":"J. Neurochem."},{"key":"10.1016\/j.neuro.2019.12.013_bib0050","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1016\/0003-2697(86)90640-8","article-title":"A direct colorimetric assay for Ca2+\u2013ATPase activity","volume":"157","author":"Chan","year":"1986","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.neuro.2019.12.013_bib0055","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1007\/BF02977347","article-title":"Adenosine and purine nucleosides prevent the disruption of mitochondrial transmembrane potential by peroxynitrite in rat primary astrocytes","volume":"28","author":"Choi","year":"2005","journal-title":"Arch. Pharm. Res."},{"key":"10.1016\/j.neuro.2019.12.013_bib0060","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10571-014-0092-3","article-title":"ATP signaling in brain: release, excitotoxicity and potential therapeutic targets","volume":"35","author":"Cisneros-Mejorado","year":"2015","journal-title":"Cell. Mol. Neurobiol."},{"key":"10.1016\/j.neuro.2019.12.013_bib0065","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s11010-013-1777-5","article-title":"Methionine and methionine sulfoxide alter parameters of oxidative stress in the liver of young rats: in vitro and in vivo studies","volume":"384","author":"Costa","year":"2013","journal-title":"Mol. Cell. Biochem."},{"key":"10.1016\/j.neuro.2019.12.013_bib0070","first-page":"233","article-title":"Hypermethioninemia due to methionine adenosyltransferase I\/III (MAT I\/III) deficiency: diagnosis in an expanded neonatal screening programme","volume":"31","author":"Couce","year":"2008","journal-title":"JIMD Rep."},{"key":"10.1016\/j.neuro.2019.12.013_bib0075","doi-asserted-by":"crossref","first-page":"85","DOI":"10.59566\/IJBS.2014.10085","article-title":"Brain Na(+), K(+)-ATPase activity in aging and disease","volume":"10","author":"de Lores Arnaiz","year":"2014","journal-title":"Int. J. Biomed. Sci."},{"key":"10.1016\/j.neuro.2019.12.013_bib0080","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s11010-016-2843-6","article-title":"Methionine and methionine sulfoxide treatment induces M1\/classical macrophage polarization and modulates oxidative stress and purinergic signaling parameters","volume":"424","author":"Dos Santos","year":"2016","journal-title":"Mol. Cell. Biochem."},{"key":"10.1016\/j.neuro.2019.12.013_bib0085","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/0006-2952(61)90145-9","article-title":"A new and rapid colorimetric determination of acetylcholinesterase activity","volume":"17","author":"Ellman","year":"1961","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.neuro.2019.12.013_bib0090","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/0076-6879(90)86134-H","article-title":"Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal","volume":"186","author":"Esterbauer","year":"1990","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.neuro.2019.12.013_bib0095","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.neulet.2013.09.056","article-title":"Nucleotide signaling in astrogliosis","volume":"17","author":"Franke","year":"2014","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.neuro.2019.12.013_bib0100","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1002\/glia.20749","article-title":"P2Y1 receptor signaling enhances neuroprotection by astrocytes against oxidative stress via IL-6 release in hippocampal cultures","volume":"57","author":"Fujita","year":"2009","journal-title":"Glia"},{"key":"10.1016\/j.neuro.2019.12.013_bib0105","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/S0006-8993(99)01503-6","article-title":"Regulation of protein phosphorylation in astrocyte cultures by external calcium ions: specific effects on the phosphorylation of glial fibrillary acidic protein (GFAP), vimentin and heat shock protein 27 (HSP27)","volume":"833","author":"Gottfried","year":"1999","journal-title":"Brain Res."},{"key":"10.1016\/j.neuro.2019.12.013_bib0110","doi-asserted-by":"crossref","first-page":"7130","DOI":"10.1016\/S0021-9258(19)42083-8","article-title":"Glutathione S-transferases. The first enzymatic step in mercapturic acid formation","volume":"249","author":"Habig","year":"1974","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.neuro.2019.12.013_bib0115","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.neuropharm.2015.08.005","article-title":"Purinergic neurone-glia signalling in cognitive-related pathologies","volume":"104","author":"Illes","year":"2016","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.neuro.2019.12.013_bib0120","doi-asserted-by":"crossref","first-page":"1966","DOI":"10.1007\/s12035-017-0463-0","article-title":"Homocysteine induces glial reactivity in adult rat astrocyte cultures","volume":"55","author":"Longoni","year":"2017","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.neuro.2019.12.013_bib0125","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.neuro.2019.12.013_bib0130","doi-asserted-by":"crossref","first-page":"3170","DOI":"10.1016\/S0021-9258(19)45228-9","article-title":"The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase","volume":"247","author":"Misra","year":"1972","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.neuro.2019.12.013_bib0135","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1523\/JNEUROSCI.4464-06.2007","article-title":"Deficiency in Na,K-ATPase alpha isoform genes alters spatial learning, motor activity, and anxiety in mice","volume":"27","author":"Moseley","year":"2007","journal-title":"J. Neurosci."},{"key":"10.1016\/j.neuro.2019.12.013_bib0140","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/ajmg.c.30293","article-title":"Hypermethioninemias of genetic and non-genetic origin: a review","volume":"157","author":"Mudd","year":"2011","journal-title":"Am. J. Med. Genet. C Semin. Med. Genet."},{"key":"10.1016\/j.neuro.2019.12.013_bib0145","series-title":"The Metabolic and Molecular Bases of Inherited Disease","first-page":"2007","article-title":"Disorders of transsulfuration","author":"Mudd","year":"2001"},{"key":"10.1016\/j.neuro.2019.12.013_bib0150","doi-asserted-by":"crossref","first-page":"225","DOI":"10.2147\/TCRM.S151732","article-title":"Methionine adenosyltransferase I\/III deficiency: beyond the central nervous system manifestations","volume":"14","author":"Nashabat","year":"2018","journal-title":"Ther. Clin. Risk Manage."},{"key":"10.1016\/j.neuro.2019.12.013_bib0155","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.neuron.2012.08.036","article-title":"Acetylcholine as a neuromodulator: cholinergic signaling shapes nervous system function and behavior","volume":"76","author":"Picciotto","year":"2012","journal-title":"Neuron"},{"key":"10.1016\/j.neuro.2019.12.013_bib0160","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1007\/s12035-017-0383-z","article-title":"Maternal hypermethioninemia affects neurons number, neurotrophins levels, energy metabolism, and Na+,K+-ATPase Expression\/Content in brain of rat offspring","volume":"55","author":"Schweinberger","year":"2018","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.neuro.2019.12.013_bib0165","doi-asserted-by":"crossref","first-page":"2479","DOI":"10.1007\/s00726-016-2302-4","article-title":"Mechanistic basis of hypermethioninemia","volume":"48","author":"Schweinberger","year":"2016","journal-title":"Amino Acids"},{"key":"10.1016\/j.neuro.2019.12.013_bib0170","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1007\/s12035-017-0383-z","article-title":"Maternal hypermethioninemia affects neurons number, neurotrophins levels, energy metabolism, and Na+,K+-ATPase expression\/content in brain of rat offspring","volume":"55","author":"Schweinberger","year":"2018","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.neuro.2019.12.013_bib0175","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1590\/0004-282X20160101","article-title":"Are astrocytes executive cells within the central nervous system?","volume":"74","author":"Sica","year":"2016","journal-title":"Arq. Neuropsiquiatr."},{"key":"10.1016\/j.neuro.2019.12.013_bib0180","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s12013-017-0815-4","article-title":"Methionine and\/or methionine sulfoxide alter ectoenzymes activities in lymphocytes and inflammatory parameters in serum from young rats: acute and chronic effects","volume":"76","author":"Soares","year":"2018","journal-title":"Cell Biochem. Biophys."},{"key":"10.1016\/j.neuro.2019.12.013_bib0185","doi-asserted-by":"crossref","first-page":"2289","DOI":"10.1002\/jcb.27554","article-title":"High levels of methionine and methionine sulfoxide: impact on adenine nucleotide hydrolysis and redox status in platelets and serum of young rats","volume":"120","author":"Soares","year":"2019","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/j.neuro.2019.12.013_bib0190","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1007\/s11011-017-0054-9","article-title":"Acute administration of methionine and\/or methionine sulfoxide impairs redox status and induces apoptosis in rat cerebral cortex","volume":"32","author":"Soares","year":"2017","journal-title":"Metab. Brain Dis."},{"key":"10.1016\/j.neuro.2019.12.013_bib0195","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s00401-009-0619-8","article-title":"Astrocytes: biology and pathology","volume":"119","author":"Sofroniew","year":"2010","journal-title":"Acta Neuropathol."},{"key":"10.1016\/j.neuro.2019.12.013_bib0200","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1007\/s11064-007-9464-0","article-title":"Hypermethioninemia increases cerebral acetylcholinesterase activity and impairs memory in rats","volume":"32","author":"Stefanello","year":"2007","journal-title":"Neurochem. Res."},{"key":"10.1016\/j.neuro.2019.12.013_bib0205","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1084\/jem.169.5.1543","article-title":"Nitric oxide. A macrophage product responsible for cytostasis and respiratory inhibition in tumor target cells","volume":"169","author":"Stuehr","year":"1989","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.neuro.2019.12.013_bib0210","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.1007\/s00429-017-1383-5","article-title":"Human astrocytes: structure and functions in the healthy brain","volume":"222","author":"Vasile","year":"2017","journal-title":"Brain Struct. Funct."},{"key":"10.1016\/j.neuro.2019.12.013_bib0215","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1007\/s12035-014-8983-3","article-title":"Methionine exposure alters glutamate uptake and adenine nucleotide hydrolysis in the zebrafish brain","volume":"53","author":"Vuaden","year":"2016","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.neuro.2019.12.013_bib0220","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1097\/00001756-200007140-00052","article-title":"Methylmalonate administration decreases Na+, K+-ATPase activity in cerebral cortex of rats","volume":"11","author":"Wyse","year":"2007","journal-title":"Neuroreport"},{"key":"10.1016\/j.neuro.2019.12.013_bib0225","doi-asserted-by":"crossref","first-page":"2641","DOI":"10.1002\/jnr.21718","article-title":"Astrocytes affect the profile of purines released from cultured cortical neurons","volume":"86","author":"Zamzow","year":"2008","journal-title":"J. Neurosci. Res."}],"container-title":["NeuroToxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0161813X19301500?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0161813X19301500?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T14:20:20Z","timestamp":1758982820000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0161813X19301500"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3]]},"references-count":45,"alternative-id":["S0161813X19301500"],"URL":"https:\/\/doi.org\/10.1016\/j.neuro.2019.12.013","relation":{},"ISSN":["0161-813X"],"issn-type":[{"type":"print","value":"0161-813X"}],"subject":[],"published":{"date-parts":[[2020,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Methionine and methionine sulfoxide induces neurochemical and morphological changes in cultured astrocytes: Involvement of Na+, K+-ATPase activity, oxidative status, and cholinergic and purinergic signaling","name":"articletitle","label":"Article Title"},{"value":"NeuroToxicology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.neuro.2019.12.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}