{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T00:22:00Z","timestamp":1794356520978,"version":"4.9.0"},"reference-count":77,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100008206","name":"Australian Nuclear Science and Technology Organisation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008206","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001164","name":"Australian Synchrotron","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001164","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["FT190100017"],"award-info":[{"award-number":["FT190100017"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP220103091"],"award-info":[{"award-number":["DP220103091"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["NeuroToxicology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.neuro.2026.103578","type":"journal-article","created":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T23:29:21Z","timestamp":1789860561000},"page":"103578","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["XANES spectroscopic analysis of frozen-hydrated brain tissue using a N2 cryo-stream best preserves In Vivo Fe and Cu speciation"],"prefix":"10.1016","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6882-934X","authenticated-orcid":false,"given":"Meg","family":"Willans","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5382-4519","authenticated-orcid":false,"given":"Gaewyn","family":"Ellison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1685-8751","authenticated-orcid":false,"given":"Kieran","family":"Reeve","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn S.","family":"Innes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7919-9977","authenticated-orcid":false,"given":"Rhiannon E.","family":"Boseley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daryl L.","family":"Howard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9369-3288","authenticated-orcid":false,"given":"Simon A.","family":"James","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3296-7270","authenticated-orcid":false,"given":"Mark J.","family":"Hackett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"8","key":"10.1016\/j.neuro.2026.103578_bib1","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1007\/s00775-019-01731-9","article-title":"Redox active metals in neurodegenerative diseases","volume":"24","author":"Acevedo","year":"2019","journal-title":"JBIC J. Biol. Inorg. Chem."},{"issue":"4","key":"10.1016\/j.neuro.2026.103578_bib2","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1007\/s12975-011-0123-z","article-title":"Decoding hippocampal signaling deficits after traumatic brain injury","volume":"2","author":"Atkins","year":"2011","journal-title":"Transl. Stroke Res."},{"issue":"19","key":"10.1016\/j.neuro.2026.103578_bib3","doi-asserted-by":"crossref","first-page":"7353","DOI":"10.1021\/ac0711135","article-title":"Subcellular speciation analysis of trace element oxidation states using synchrotron radiation micro-X-ray absorption near-edge structure","volume":"79","author":"Bacquart","year":"2007","journal-title":"Anal. Chem."},{"key":"10.1016\/j.neuro.2026.103578_bib4","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1038\/nrd1330","article-title":"Neurodegenerative diseases and oxidative stress","volume":"3","author":"Barnham","year":"2004","journal-title":"Nat. Rev. Drug. Discov."},{"key":"10.1016\/j.neuro.2026.103578_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.jtemb.2020.126555","article-title":"Analysis of neuronal iron deposits in Parkinson's disease brain tissue by synchrotron x-ray spectromicroscopy","volume":"62","author":"Brooks","year":"2020","journal-title":"J. Trace Elem. Med. Biol."},{"issue":"2","key":"10.1016\/j.neuro.2026.103578_bib6","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/S1367-5931(99)00073-3","article-title":"Metals and neuroscience","volume":"4","author":"Bush","year":"2000","journal-title":"Curr. Opin. Chem. Biol."},{"issue":"2","key":"10.1016\/j.neuro.2026.103578_bib7","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1021\/jacs.1c03984","article-title":"Evidence for a long-lived, cu-coupled and oxygen-inert disulfide radical anion in the assembly of metallothionein-3 Cu(I)(4)-thiolate cluster","volume":"144","author":"Calvo","year":"2022","journal-title":"J. Am. Chem. Soc."},{"issue":"2","key":"10.1016\/j.neuro.2026.103578_bib8","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1007\/s00775-006-0179-5","article-title":"Investigations of differences in iron oxidation state inside single neurons from substantia nigra of Parkinson's disease and control patients using the micro-XANES technique","volume":"12","author":"Chwiej","year":"2007","journal-title":"JBIC J. Biol. Inorg. Chem."},{"issue":"2","key":"10.1016\/j.neuro.2026.103578_bib9","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s00775-010-0724-0","article-title":"X-ray fluorescence analysis of long-term changes in the levels and distributions of trace elements in the rat brain following mechanical injury","volume":"16","author":"Chwiej","year":"2011","journal-title":"JBIC J. Biol. Inorg. Chem."},{"issue":"12","key":"10.1016\/j.neuro.2026.103578_bib10","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1016\/j.sab.2005.10.002","article-title":"Preparation of tissue samples for X-ray fluorescence microscopy","volume":"60","author":"Chwiej","year":"2005","journal-title":"Spectrochim. Acta Part. B-At. Spectrosc."},{"key":"10.1016\/j.neuro.2026.103578_bib11","doi-asserted-by":"crossref","first-page":"1894","DOI":"10.1039\/C4MT00176A","article-title":"Quantitation and localization of intracellular redox active metals by X-ray fluorescence microscopy in cortical neurons derived from APP and APLP2 knockout tissue","volume":"6","author":"Ciccotosto","year":"2014","journal-title":"Metallomics"},{"issue":"4","key":"10.1016\/j.neuro.2026.103578_bib12","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.neurobiolaging.2013.09.034","article-title":"Copper pathology in vulnerable brain regions in Parkinson's disease","volume":"35","author":"Davies","year":"2014","journal-title":"Neurobiol. Aging"},{"issue":"7","key":"10.1016\/j.neuro.2026.103578_bib13","doi-asserted-by":"crossref","DOI":"10.1093\/mtomcs\/mfaf022","article-title":"Rust and redemption: iron\u2013sulfur clusters and oxygen in human disease and health","volume":"17","author":"Egozi","year":"2025","journal-title":"Metallomics"},{"issue":"3","key":"10.1016\/j.neuro.2026.103578_bib14","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1021\/cbmi.5c00059","article-title":"Integrating Microscopy Methods to Study Gene and Protein Expression alongside Metal Ion Distribution and Speciation: A Case Study of Iron within Pyramidal Neurons from Distinct Hippocampal CA1 Subregions","volume":"4","author":"Ellison","year":"2026","journal-title":"Chem. & Biomed. Imaging"},{"issue":"1","key":"10.1016\/j.neuro.2026.103578_bib15","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-67117-z","article-title":"Iron stored in ferritin is chemically reduced in the presence of aggregating Abeta(1-42)","volume":"10","author":"Everett","year":"2020","journal-title":"Sci. Rep."},{"issue":"25","key":"10.1016\/j.neuro.2026.103578_bib16","doi-asserted-by":"crossref","first-page":"11782","DOI":"10.1039\/C7NR06794A","article-title":"Nanoscale synchrotron X-ray speciation of iron and calcium compounds in amyloid plaque cores from Alzheimer's disease subjects","volume":"10","author":"Everett","year":"2018","journal-title":"Nanoscale"},{"issue":"24","key":"10.1016\/j.neuro.2026.103578_bib17","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abf6707","article-title":"Biogenic metallic elements in the human brain?","volume":"7","author":"Everett","year":"2021","journal-title":"Sci. Adv."},{"issue":"10","key":"10.1016\/j.neuro.2026.103578_bib18","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1039\/C7MT00244K","article-title":"Subcellular compartmentalisation of copper, iron, manganese, and zinc in the Parkinson's disease brain","volume":"9","author":"Genoud","year":"2017","journal-title":"Metallomics"},{"issue":"6","key":"10.1016\/j.neuro.2026.103578_bib19","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1107\/S090904951203943X","article-title":"X-ray-induced photo-chemistry and X-ray absorption spectroscopy of biological samples","volume":"19","author":"George","year":"2012","journal-title":"J. Synchrotron Radiat."},{"key":"10.1016\/j.neuro.2026.103578_bib20","unstructured":"George, G.N.; Pickering, I.J. EXAFSPAK: A suite of computer programs for analysis of X-ray absorption spectra. http:\/\/ssrl.slac.stanford.edu\/exafspak.html 1995."},{"issue":"6","key":"10.1016\/j.neuro.2026.103578_bib21","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.4155\/fmc.09.84","article-title":"Human copper transporters: mechanism, role in human diseases and therapeutic potential","volume":"1","author":"Gupta","year":"2009","journal-title":"Future Med. Chem."},{"key":"10.1016\/j.neuro.2026.103578_bib22","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1021\/cn500157j","article-title":"situ Bio-Spectroscopic Investigation of Rapid Ischemic and Post-mortem Induced Biochemical Alterations in the Rat Brain","volume":"6","author":"Hackett","year":"2015","journal-title":"ACS Chem. Neurosci."},{"key":"10.1016\/j.neuro.2026.103578_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.clispe.2021.100017","article-title":"A spectroscopic picture paints 1000 words\u201d mapping iron speciation in brain tissue with \u201cfull spectrum per pixel\u201d X-ray absorption near-edge structure spectroscopy","volume":"3","author":"Hackett","year":"2021","journal-title":"Clin. Spectrosc."},{"issue":"1","key":"10.1016\/j.neuro.2026.103578_bib24","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1039\/C8MT00230D","article-title":"Elemental characterisation of the pyramidal neuron layer within the rat and mouse hippocampus","volume":"11","author":"Hackett","year":"2019","journal-title":"Metallomics"},{"issue":"14","key":"10.1016\/j.neuro.2026.103578_bib25","doi-asserted-by":"crossref","first-page":"2941","DOI":"10.1039\/c0an00269k","article-title":"Chemical alterations to murine brain tissue induced by formalin fixation: implications for biospectroscopic imaging and mapping studies of disease pathogenesis","volume":"136","author":"Hackett","year":"2011","journal-title":"Analyst"},{"issue":"3","key":"10.1016\/j.neuro.2026.103578_bib26","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1021\/cn200097s","article-title":"X-ray Absorption Spectroscopy at the Sulfur K-Edge: A New Tool to Investigate the Biochemical Mechanisms of Neurodegeneration","volume":"3","author":"Hackett","year":"2012","journal-title":"ACS Chem. Neurosci."},{"key":"10.1016\/j.neuro.2026.103578_bib27","doi-asserted-by":"crossref","DOI":"10.3389\/fnagi.2013.00034","article-title":"A delicate balance: Iron metabolism and diseases of the brain","volume":"5","author":"Hare","year":"2013","journal-title":"Front. Aging Neurosci."},{"issue":"11","key":"10.1016\/j.neuro.2026.103578_bib28","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1039\/c0mt00039f","article-title":"Three-dimensional elemental bio-imaging of Fe, Zn, Cu, Mn and P in a 6-hydroxydopamine lesioned mouse brain","volume":"2","author":"Hare","year":"2010","journal-title":"Metallomics"},{"issue":"1","key":"10.1016\/j.neuro.2026.103578_bib29","doi-asserted-by":"crossref","DOI":"10.1093\/mtomcs\/mfad072","article-title":"Evidence for decreased copper associated with demyelination in the corpus callosum of cuprizone-treated mice","volume":"16","author":"Hilton","year":"2024","journal-title":"Metallomics"},{"issue":"3","key":"10.1016\/j.neuro.2026.103578_bib30","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1021\/acschemneuro.4c00320","article-title":"Subventricular Accumulation of Cu in the Aging Mouse Brain Does Not Associate with Anticipated Increases in Markers of Oxidative Stress","volume":"16","author":"Hollings","year":"2025","journal-title":"ACS Chem. Neurosci."},{"issue":"12","key":"10.1016\/j.neuro.2026.103578_bib31","doi-asserted-by":"crossref","first-page":"2134","DOI":"10.1039\/d0mt00198h","article-title":"Revealing differences in the chemical form of zinc in brain tissue using K-edge X-ray absorption near-edge structure spectroscopy","volume":"12","author":"Hollings","year":"2020","journal-title":"Metallomics"},{"issue":"1","key":"10.1016\/j.neuro.2026.103578_bib32","doi-asserted-by":"crossref","DOI":"10.1093\/mtomcs\/mfaf045","article-title":"Imaging zinc speciation in the mouse hippocampus with \u00b5XANES Spectroscopic mapping","volume":"18","author":"Hollings","year":"2026","journal-title":"Metallomics"},{"issue":"Pt 5","key":"10.1016\/j.neuro.2026.103578_bib33","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1107\/S1600577520010152","article-title":"The XFM beamline at the Australian Synchrotron","volume":"27","author":"Howard","year":"2020","journal-title":"J. Synchrotron Radiat."},{"issue":"1","key":"10.1016\/j.neuro.2026.103578_bib34","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1196\/annals.1306.012","article-title":"Redox-Active Metals, Oxidative Stress, and Alzheimer's Disease Pathology","volume":"1012","author":"Huang","year":"2004","journal-title":"Ann. New. Y. Acad. Sci."},{"issue":"9","key":"10.1016\/j.neuro.2026.103578_bib35","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1042\/BC20110015","article-title":"The future is cold: Cryo-preparation methods for transmission electron microscopy of cells","volume":"103","author":"Hurbain","year":"2011","journal-title":"Biol. Cell."},{"issue":"4","key":"10.1016\/j.neuro.2026.103578_bib36","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1111\/j.1365-2958.2006.05028.x","article-title":"Iron-sulphur clusters and the problem with oxygen","volume":"59","author":"Imlay","year":"2006","journal-title":"Mol. Microbiol."},{"issue":"79","key":"10.1016\/j.neuro.2026.103578_bib37","doi-asserted-by":"crossref","first-page":"11834","DOI":"10.1039\/C6CC06747F","article-title":"Visualising coordination chemistry: fluorescence X-ray absorption near edge structure tomography","volume":"52","author":"James","year":"2016","journal-title":"Chem. Commun. (Camb. )"},{"key":"10.1016\/j.neuro.2026.103578_bib38","doi-asserted-by":"crossref","first-page":"20350","DOI":"10.1038\/srep20350","article-title":"XANES: In vivo imaging of metal-protein coordination environments","volume":"6","author":"James","year":"2016","journal-title":"Sci. Rep."},{"issue":"5","key":"10.1016\/j.neuro.2026.103578_bib39","doi-asserted-by":"crossref","first-page":"2952","DOI":"10.1039\/C5SC00233H","article-title":"Direct in vivo imaging of ferrous iron dyshomeostasis in ageing Caenorhabditis elegans","volume":"6","author":"James","year":"2015","journal-title":"Chem. Sci."},{"issue":"2","key":"10.1016\/j.neuro.2026.103578_bib40","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.freeradbiomed.2011.10.446","article-title":"Elevated labile Cu is associated with oxidative pathology in Alzheimer disease","volume":"52","author":"James","year":"2012","journal-title":"Free. Radic. Biol. Med."},{"key":"10.1016\/j.neuro.2026.103578_bib41","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.56580","article-title":"Changes in ferrous iron and glutathione promote ferroptosis and frailty in aging Caenorhabditis elegans","volume":"9","author":"Jenkins","year":"2020","journal-title":"Elife"},{"key":"10.1016\/j.neuro.2026.103578_bib42","doi-asserted-by":"crossref","DOI":"10.1093\/mtomcs\/mfac092","article-title":"Characterisation of the spatial distribution of metals and profile of metalloprotein complexes in a mouse model of repetitive mild traumatic brain injury","author":"Juan","year":"2022","journal-title":"Metallomics"},{"issue":"9","key":"10.1016\/j.neuro.2026.103578_bib43","doi-asserted-by":"crossref","first-page":"7401","DOI":"10.1021\/acsnano.5c10637","article-title":"Cryo-correlative light and X-ray microscopies: expanding the intracellular chemical map","volume":"20","author":"Karpov","year":"2026","journal-title":"ACS nano"},{"issue":"18","key":"10.1016\/j.neuro.2026.103578_bib44","doi-asserted-by":"crossref","first-page":"11098","DOI":"10.1016\/S0021-9258(17)44390-0","article-title":"The role of iron in the activation-inactivation of aconitase","volume":"258","author":"Kennedy","year":"1983","journal-title":"J. Biol. Chem."},{"issue":"3","key":"10.1016\/j.neuro.2026.103578_bib45","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1073\/pnas.97.3.1050","article-title":"A mitochondrial ferredoxin is essential for biogenesis of cellular iron-sulfur proteins","volume":"97","author":"Lange","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"1","key":"10.1016\/j.neuro.2026.103578_bib46","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.neuroimage.2010.11.073","article-title":"Increased brain iron coincides with early plaque formation in a mouse model of Alzheimer's disease","volume":"55","author":"Leskovjan","year":"2011","journal-title":"NeuroImage"},{"issue":"6","key":"10.1016\/j.neuro.2026.103578_bib47","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/j.cell.2004.11.003","article-title":"The Importance of Dendritic Mitochondria in the Morphogenesis and Plasticity of Spines and Synapses","volume":"119","author":"Li","year":"2004","journal-title":"Cell"},{"issue":"6","key":"10.1016\/j.neuro.2026.103578_bib48","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0158152","article-title":"Mapping Alterations to the Endogenous Elemental Distribution within the Lateral Ventricles and Choroid Plexus in Brain Disorders Using X-Ray Fluorescence Imaging","volume":"11","author":"Lins","year":"2016","journal-title":"PLoS. One"},{"issue":"4","key":"10.1016\/j.neuro.2026.103578_bib49","doi-asserted-by":"crossref","DOI":"10.1093\/mtomcs\/mfaf009","article-title":"Correlative analysis of metallomic gene expression and metal ion content within the mouse hippocampus","volume":"17","author":"Mamsa","year":"2025","journal-title":"Metallomics"},{"issue":"5","key":"10.1016\/j.neuro.2026.103578_bib50","doi-asserted-by":"crossref","DOI":"10.1093\/braincomms\/fcac215","article-title":"Hippocampal glutathione depletion with enhanced iron level in patients with mild cognitive impairment and Alzheimer's disease compared with healthy elderly participants","volume":"4","author":"Mandal","year":"2022","journal-title":"Brain Commun."},{"issue":"4","key":"10.1016\/j.neuro.2026.103578_bib51","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1002\/xrs.1256","article-title":"Elemental mapping of frozen-hydrated cells with cryo-scanning X-ray fluorescence microscopy","volume":"39","author":"Matsuyama","year":"2010","journal-title":"X-Ray Spectrom."},{"issue":"1","key":"10.1016\/j.neuro.2026.103578_bib52","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1146\/annurev.biophys.27.1.329","article-title":"Cytochrome c oxidase: structure and spectroscopy","volume":"27","author":"Michel","year":"1998","journal-title":"Annu. Rev. Biophys. Biomol. Struct."},{"issue":"2","key":"10.1016\/j.neuro.2026.103578_bib53","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.neuron.2014.12.032","article-title":"Blood-Brain Barrier Breakdown in the Aging Human Hippocampus","volume":"85","author":"Montagne","year":"2015","journal-title":"Neuron"},{"issue":"11","key":"10.1016\/j.neuro.2026.103578_bib54","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1021\/cbmi.4c00038","article-title":"Native cryo-correlative light and synchrotron X-ray fluorescence imaging of proteins and essential metals in subcellular neuronal compartments","volume":"2","author":"Ortega","year":"2024","journal-title":"Chem. & Biomed. Imaging"},{"issue":"1-2","key":"10.1016\/j.neuro.2026.103578_bib55","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.ccr.2004.03.011","article-title":"Characterization of \"spectroscopically quiet\" metals in biology","volume":"249","author":"Penner-Hahn","year":"2005","journal-title":"Coord. Chem. Rev."},{"key":"10.1016\/j.neuro.2026.103578_bib56","first-page":"1101","article-title":"Penner-Hahn, JE. X-ray absorption spectroscopy in coordination chemistry","volume":"190","year":"1999","journal-title":"Coord. Chem. Rev."},{"issue":"1","key":"10.1016\/j.neuro.2026.103578_bib57","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s00401-017-1696-8","article-title":"Pathogenic implications of distinct patterns of iron and zinc in chronic MS lesions","volume":"134","author":"Popescu","year":"2017","journal-title":"Acta Neuropathol."},{"issue":"3","key":"10.1016\/j.neuro.2026.103578_bib58","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1088\/0031-9155\/54\/3\/012","article-title":"Mapping metals in Parkinson's and normal brain using rapid-scanning x-ray fluorescence","volume":"54","author":"Popescu","year":"2009","journal-title":"Phys. Med. Biol."},{"issue":"2","key":"10.1016\/j.neuro.2026.103578_bib59","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1039\/C5MT00234F","article-title":"A time-course analysis of changes in cerebral metal levels following a controlled cortical impact","volume":"8","author":"Portbury","year":"2016","journal-title":"Metallomics"},{"issue":"11","key":"10.1016\/j.neuro.2026.103578_bib60","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1039\/D0JA00323A","article-title":"Sample preparation with sucrose cryoprotection dramatically alters Zn distribution in the rodent hippocampus, as revealed by elemental mapping","volume":"35","author":"Pushie","year":"2020","journal-title":"J. Anal. At. Spectrom."},{"issue":"1","key":"10.1016\/j.neuro.2026.103578_bib61","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-74698-2","article-title":"Tracking elemental changes in an ischemic stroke model with X-ray fluorescence imaging","volume":"10","author":"Pushie","year":"2020","journal-title":"Sci. Rep."},{"issue":"17","key":"10.1016\/j.neuro.2026.103578_bib62","doi-asserted-by":"crossref","first-page":"8499","DOI":"10.1021\/cr4007297","article-title":"Elemental and chemically specific X-ray fluorescence imaging of biological systems","volume":"114","author":"Pushie","year":"2014","journal-title":"Chem. Rev."},{"issue":"2","key":"10.1016\/j.neuro.2026.103578_bib63","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1039\/c0mt00037j","article-title":"Prion protein expression level alters regional copper, iron and zinc content in the mouse brain","volume":"3","author":"Pushie","year":"2011","journal-title":"Metallomics"},{"issue":"6","key":"10.1016\/j.neuro.2026.103578_bib64","doi-asserted-by":"crossref","DOI":"10.1093\/mtomcs\/mfac032","article-title":"X-ray fluorescence microscopy methods for biological tissues","volume":"14","author":"Pushie","year":"2022","journal-title":"Metallomics"},{"issue":"5","key":"10.1016\/j.neuro.2026.103578_bib65","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1111\/acel.12112","article-title":"Aging results in copper accumulations in glial fibrillary acidic protein-positive cells in the subventricular zone","volume":"12","author":"Pushkar","year":"2013","journal-title":"Aging Cell."},{"issue":"5415","key":"10.1016\/j.neuro.2026.103578_bib66","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1126\/science.284.5415.805","article-title":"Undetectable intracellular free copper: the requirement of a copper chaperone for superoxide dismutase","volume":"284","author":"Rae","year":"1999","journal-title":"Science"},{"issue":"3","key":"10.1016\/j.neuro.2026.103578_bib67","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23031634","article-title":"High Iron and Iron Household Protein Contents in Perineuronal Net-Ensheathed Neurons Ensure Energy Metabolism with Safe Iron Handling","volume":"23","author":"Reinert","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"7","key":"10.1016\/j.neuro.2026.103578_bib68","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1038\/s41589-018-0089-1","article-title":"Copper on the brain","volume":"14","author":"Rihel","year":"2018","journal-title":"Nat. Chem. Biol."},{"issue":"1","key":"10.1016\/j.neuro.2026.103578_bib69","article-title":"MAIA X-ray fluorescence imaging: capturing detail in complex natural samples","volume":"499","author":"Ryan","year":"2014","journal-title":"J. Phys. Conf. Ser."},{"key":"10.1016\/j.neuro.2026.103578_bib70","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.redox.2016.12.007","article-title":"On the nature of the Cu-rich aggregates in brain astrocytes","volume":"11","author":"Sullivan","year":"2017","journal-title":"Redox Biol."},{"key":"10.1016\/j.neuro.2026.103578_bib71","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.jtemb.2016.06.011","article-title":"Copper accumulation in rodent brain astrocytes: A species difference","volume":"39","author":"Sullivan","year":"2017","journal-title":"J. Trace Elem. Med. Biol."},{"key":"10.1016\/j.neuro.2026.103578_bib72","series-title":"Postsynaptic mitochondria are positioned to support functional diversity of dendritic spines","author":"Thomas","year":"2023"},{"issue":"5","key":"10.1016\/j.neuro.2026.103578_bib73","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1002\/glia.20784","article-title":"Oligodendrocytes and myelination: the role of iron","volume":"57","author":"Todorich","year":"2009","journal-title":"Glia"},{"key":"10.1016\/j.neuro.2026.103578_bib74","doi-asserted-by":"crossref","DOI":"10.1177\/13872877261435561","article-title":"Characterizing ferrous versus ferric iron in Alzheimer's disease using X-ray fluorescence imaging and XANES spectroscopy","author":"Tran","year":"2026","journal-title":"J. Alzheimer\u2019S. Dis."},{"issue":"2","key":"10.1016\/j.neuro.2026.103578_bib75","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/S1053-8119(03)00361-6","article-title":"Changes in hippocampal volume and shape across time distinguish dementia of the Alzheimer type from healthy aging\u2606","volume":"20","author":"Wang","year":"2003","journal-title":"NeuroImage"},{"issue":"9","key":"10.1016\/j.neuro.2026.103578_bib76","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1039\/C4MT00088A","article-title":"XAS and XFM studies of selenium and copper speciation and distribution in the kidneys of selenite-supplemented rats","volume":"6","author":"Weekley","year":"2014","journal-title":"Metallomics"},{"key":"10.1016\/j.neuro.2026.103578_bib77","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1161\/STROKEAHA.116.015404","article-title":"Rehabilitation Augments Hematoma Clearance and Attenuates Oxidative Injury and Ion Dyshomeostasis After Brain Hemorrhage","volume":"48","author":"Williamson","year":"2016","journal-title":"Stroke"}],"container-title":["NeuroToxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0161813X26001993?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0161813X26001993?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T23:39:03Z","timestamp":1794353943000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0161813X26001993"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":77,"alternative-id":["S0161813X26001993"],"URL":"https:\/\/doi.org\/10.1016\/j.neuro.2026.103578","relation":{},"ISSN":["0161-813X"],"issn-type":[{"value":"0161-813X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"XANES spectroscopic analysis of frozen-hydrated brain tissue using a N2 cryo-stream best preserves In Vivo Fe and Cu speciation","name":"articletitle","label":"Article Title"},{"value":"NeuroToxicology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.neuro.2026.103578","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"103578"}}