{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:03:16Z","timestamp":1790683396294,"version":"4.1.0"},"reference-count":146,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100010767","name":"Innovative Medicines Initiative","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010767","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UK Medical Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["106713\/Z\/14\/Z"],"award-info":[{"award-number":["106713\/Z\/14\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Neurobiology of Aging"],"published-print":{"date-parts":[[2019,6]]},"DOI":"10.1016\/j.neurobiolaging.2019.02.008","type":"journal-article","created":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T06:44:59Z","timestamp":1550731499000},"page":"130-141","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":51,"special_numbering":"C","title":["Using stem cell\u2013derived neurons in drug screening for neurological diseases"],"prefix":"10.1016","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8806-3484","authenticated-orcid":false,"given":"Daniel","family":"Little","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Ketteler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9712-6122","authenticated-orcid":false,"given":"Paul","family":"Gissen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Devine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib1","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.neuron.2017.03.042","article-title":"iPSC-derived human microglia-like cells to study neurological diseases","volume":"94","author":"Abud","year":"2017","journal-title":"Neuron"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib2","doi-asserted-by":"crossref","first-page":"2062","DOI":"10.1002\/sctm.17-0150","article-title":"Concise review: induced pluripotent stem cell models for neuropsychiatric diseases","volume":"6","author":"Adegbola","year":"2017","journal-title":"Stem Cell. Transl. Med."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib3","doi-asserted-by":"crossref","first-page":"300","DOI":"10.3389\/fgene.2015.00300","article-title":"A\u00a0new age in functional genomics using CRISPR\/Cas9 in arrayed library screening","volume":"6","author":"Agrotis","year":"2015","journal-title":"Front. Genet."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib4","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.copbio.2017.02.006","article-title":"Optogenetic methods in drug screening: technologies and applications","volume":"48","author":"Agus","year":"2017","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib5","series-title":"Bringing the Light to High Throughput Screening: Use of Optogenetic Tools for the Development of Recombinant Cellular Assays","author":"Agus","year":"2015"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib6","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.ymeth.2015.06.004","article-title":"PALM and STORM: into large fields and high-throughput microscopy with sCMOS detectors","volume":"88","author":"Almada","year":"2015","journal-title":"Methods"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib7","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s13195-017-0268-4","article-title":"Stem cell models of Alzheimer's disease: progress and challenges","volume":"9","author":"Arber","year":"2017","journal-title":"Alzheimers Res. Ther."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib8","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1007\/s00449-016-1659-9","article-title":"Comparability of automated human induced pluripotent stem cell culture: a pilot study","volume":"39","author":"Archibald","year":"2016","journal-title":"Bioproc. Biosyst. Eng."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib9","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1203\/PDR.0b013e31822e1825","article-title":"Kinetin improves IKBKAP mRNA splicing in patients with familial dysautonomia","volume":"70","author":"Axelrod","year":"2011","journal-title":"Pediatr. Res."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib10","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.pneurobio.2013.04.003","article-title":"Scientific and ethical issues related to stem cell research and interventions in neurodegenerative disorders of the brain","volume":"110","author":"Barker","year":"2013","journal-title":"Prog. Neurobiol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib11","doi-asserted-by":"crossref","first-page":"78","DOI":"10.3389\/fnmol.2016.00078","article-title":"Utility of induced pluripotent stem cells for the study and treatment of genetic diseases: focus on childhood neurological disorders","volume":"9","author":"Barral","year":"2016","journal-title":"Front. Mol. Neurosci."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib12","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1038\/s41586-018-0409-3","article-title":"Genetic and transcriptional evolution alters cancer cell line drug response","volume":"560","author":"Ben-David","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib13","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1038\/nature09915","article-title":"Modelling schizophrenia using human induced pluripotent stem cells","volume":"473","author":"Brennand","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib14","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1016\/j.stemcr.2017.02.006","article-title":"Phenotypic screening identifies modulators of amyloid precursor protein processing in human stem cell models of Alzheimer's disease","volume":"8","author":"Brownjohn","year":"2017","journal-title":"Stem Cell. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib15","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.mcn.2013.07.007","article-title":"A\u00a0cellular model for sporadic ALS using patient-derived induced pluripotent stem cells","volume":"56","author":"Burkhardt","year":"2013","journal-title":"Mol. Cell. Neurosci"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib16","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.gpb.2017.07.003","article-title":"Deep learning and its applications in biomedicine","volume":"16","author":"Cao","year":"2018","journal-title":"Genom. Proteom. Bioinform."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib17","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1089\/scd.2007.0022","article-title":"Identification of small molecules from human embryonic stem cells using metabolomics","volume":"16","author":"Cezar","year":"2007","journal-title":"Stem Cell. Dev."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib18","first-page":"21.8.1","article-title":"Highly expandable human iPS cell-derived neural progenitor cells (NPC) and neurons for central nervous system disease modeling and high-throughput screening","volume":"92","author":"Cheng","year":"2017","journal-title":"Curr. Protoc. Hum. Genet."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib19","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s00441-017-2749-y","article-title":"iPS cells in the study of PD molecular pathogenesis","volume":"373","author":"Cobb","year":"2018","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib20","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.stem.2012.04.027","article-title":"Induced pluripotent stem cells from patients with Huntington's disease show CAG-repeat-expansion-associated phenotypes","volume":"11","author":"Consortium","year":"2012","journal-title":"Cell. Stem Cell."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib21","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.brainres.2015.11.013","article-title":"Back and forth in time: directing age in iPSC-derived lineages","volume":"1656","author":"Cornacchia","year":"2015","journal-title":"Brain Res."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib22","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.biocel.2018.05.014","article-title":"SRRF: universal live-cell super-resolution microscopy","volume":"101","author":"Culley","year":"2018","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib23","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1177\/1087057116670068","article-title":"One-step seeding of neural stem cells with vitronectin-supplemented medium for high-throughput screening assays","volume":"21","author":"Dai","year":"2016","journal-title":"J.\u00a0Biomol. Screen."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib24","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1177\/2472630317712220","article-title":"Automated cell culture systems and their applications to human pluripotent stem cell studies","volume":"23","author":"Daniszewski","year":"2018","journal-title":"SLAS Technol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib25","first-page":"270","article-title":"IGF1 neuronal response in the absence of MECP2 is dependent on TRalpha 3","volume":"26","author":"de Souza","year":"2017","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib26","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S1016-8478(23)10761-8","article-title":"Genome-wide examination of chromosomal aberrations in neuroblastoma SH-SY5Y cells by array-based comparative genomic hybridization","volume":"24","author":"Do","year":"2007","journal-title":"Mol. Cell"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib27","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.stemcr.2014.01.013","article-title":"Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation","volume":"2","author":"Doi","year":"2014","journal-title":"Stem Cell. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib28","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1016\/j.stemcr.2017.04.023","article-title":"Directed differentiation of human pluripotent stem cells to microglia","volume":"8","author":"Douvaras","year":"2017","journal-title":"Stem Cell. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib29","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.stemcr.2017.12.021","article-title":"Astrocytes attenuate mitochondrial dysfunctions in human dopaminergic neurons derived from iPSC","volume":"10","author":"Du","year":"2018","journal-title":"Stem Cell. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib30","doi-asserted-by":"crossref","first-page":"437","DOI":"10.5966\/sctm.2014-0137","article-title":"Human pluripotent stem cell-derived radial glia recapitulate developmental events and provide real-time access to cortical neurons and astrocytes","volume":"4","author":"Duan","year":"2015","journal-title":"Stem Cell. Transl. Med."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib31","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1038\/nature07677","article-title":"Induced pluripotent stem cells from a spinal muscular atrophy patient","volume":"457","author":"Ebert","year":"2008","journal-title":"Nature"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib32","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1177\/1087057113501869","article-title":"Functional screening assays with neurons generated from pluripotent stem cell-derived neural stem cells","volume":"19","author":"Efthymiou","year":"2014","journal-title":"J.\u00a0Biomol. Screen."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib33","doi-asserted-by":"crossref","first-page":"560","DOI":"10.2174\/138620708785204144","article-title":"The use of immortalized cell lines in GPCR screening: the good, bad and ugly","volume":"11","author":"Eglen","year":"2008","journal-title":"Comb. Chem. High Throughput Screen."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib34","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1089\/scd.2010.0560","article-title":"Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes","volume":"21","author":"Emdad","year":"2012","journal-title":"Stem Cell. Dev."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib35","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.neuron.2018.10.033","article-title":"Best practices for translational disease modeling using human iPSC-derived neurons","volume":"100","author":"Engle","year":"2018","journal-title":"Neuron"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib36","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.stem.2018.05.019","article-title":"Toward precision medicine for neurological and neuropsychiatric disorders","volume":"23","author":"Gibbs","year":"2018","journal-title":"Cell. Stem Cell."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib37","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.pediatrneurol.2017.07.002","article-title":"A\u00a0double-blind, randomized, placebo-controlled clinical study of Trofinetide in the treatment of rett syndrome","volume":"76","author":"Glaze","year":"2017","journal-title":"Pediatr. Neurol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nrd3577","article-title":"Induced pluripotent stem cells \u2014 opportunities for disease modelling and drug discovery","volume":"10","author":"Grskovic","year":"2011","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib39","doi-asserted-by":"crossref","first-page":"671","DOI":"10.3389\/fnins.2017.00671","article-title":"Current advances and limitations in modeling ALS\/FTD in a dish using induced pluripotent stem cells","volume":"11","author":"Guo","year":"2017","journal-title":"Front. Neurosci."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib40","doi-asserted-by":"crossref","first-page":"12471","DOI":"10.1038\/ncomms12471","article-title":"Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations","volume":"7","author":"Gustafsson","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib41","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1016\/j.stemcr.2017.05.017","article-title":"A\u00a0highly efficient human pluripotent stem cell microglia model displays a neuronal-co-culture-specific expression profile and inflammatory response","volume":"8","author":"Haenseler","year":"2017","journal-title":"Stem Cell. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib42","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1038\/nbt.1957","article-title":"Astrocytes from familial and sporadic ALS patients are toxic to motor neurons","volume":"29","author":"Haidet-Phillips","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib43","doi-asserted-by":"crossref","first-page":"e7155","DOI":"10.1371\/journal.pone.0007155","article-title":"Identification by automated screening of a small molecule that selectively eliminates neural stem cells derived from hESCs but not dopamine neurons","volume":"4","author":"Han","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib44","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1093\/hmg\/ddv637","article-title":"Assessing similarity to primary tissue and cortical layer identity in induced pluripotent stem cell-derived cortical neurons through single-cell transcriptomics","volume":"25","author":"Handel","year":"2016","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib45","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1038\/nbt.2786","article-title":"Clinical development success rates for investigational drugs","volume":"32","author":"Hay","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib46","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.tiv.2018.06.002","article-title":"Culture of human neurospheres in 3D scaffolds for developmental neurotoxicity testing","volume":"52","author":"Hellwig","year":"2018","journal-title":"Toxicol. Vitro"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib47","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/j.stem.2012.07.005","article-title":"Discovery of inhibitors of microglial neurotoxicity acting through multiple mechanisms using a stem-cell-based phenotypic assay","volume":"11","author":"Hoing","year":"2012","journal-title":"Cell. Stem Cell."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib48","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1038\/mt.2012.255","article-title":"Assessing the risks of genotoxicity in the therapeutic development of induced pluripotent stem cells","volume":"21","author":"Hong","year":"2013","journal-title":"Mol. Ther."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib49","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/nrd.2016.175","article-title":"Screening out irrelevant cell-based models of disease","volume":"15","author":"Horvath","year":"2016","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib50","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jneumeth.2016.03.017","article-title":"Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells","volume":"266","author":"Hu","year":"2016","journal-title":"J.\u00a0Neurosci. Methods"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib51","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1002\/stem.2637","article-title":"Concise review: induced pluripotent stem cell-based drug discovery for mitochondrial disease","volume":"35","author":"Inak","year":"2017","journal-title":"Stem Cell."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib52","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.bej.2015.09.024","article-title":"Patient-specific hiPSC bioprocessing for drug screening: bioprocess economics and optimisation","volume":"108","author":"Jenkins","year":"2016","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib53","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/1756-6606-5-17","article-title":"Astrocytes generated from patient induced pluripotent stem cells recapitulate features of Huntington's disease patient cells","volume":"5","author":"Juopperi","year":"2012","journal-title":"Mol. Brain"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib54","doi-asserted-by":"crossref","first-page":"2551","DOI":"10.1039\/C6LC00489J","article-title":"Microfluidic systems for stem cell-based neural tissue engineering","volume":"16","author":"Karimi","year":"2016","journal-title":"Lab Chip."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib55","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1177\/1087057115588287","article-title":"High-throughput screening using iPSC-derived neuronal progenitors to identify compounds counteracting epigenetic gene silencing in fragile X syndrome","volume":"20","author":"Kaufmann","year":"2015","journal-title":"J.\u00a0Biomol. Screen."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib56","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.stemcr.2015.11.013","article-title":"Grafted human iPS cell-derived oligodendrocyte precursor cells contribute to robust remyelination of demyelinated axons after spinal cord injury","volume":"6","author":"Kawabata","year":"2016","journal-title":"Stem Cell. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib57","series-title":"Encyclopedia of Cell Biology","first-page":"234","article-title":"High-content screening in cell biology","volume":"4","author":"Ketteler","year":"2016"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib58","doi-asserted-by":"crossref","first-page":"e39715","DOI":"10.1371\/journal.pone.0039715","article-title":"Highly pure and expandable PSA-NCAM-positive neural precursors from human ESC and iPSC-derived neural rosettes","volume":"7","author":"Kim","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib59","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1038\/nprot.2015.065","article-title":"A\u00a03D human neural cell culture system for modeling Alzheimer's disease","volume":"10","author":"Kim","year":"2015","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib60","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.celrep.2012.04.009","article-title":"Generation of regionally specified neural progenitors and functional neurons from human embryonic stem cells under defined conditions","volume":"1","author":"Kirkeby","year":"2012","journal-title":"Cell Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib61","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.stem.2016.09.004","article-title":"Predictive markers guide differentiation to improve graft outcome in clinical translation of hESC-based therapy for Parkinson\u2019s disease","volume":"20","author":"Kirkeby","year":"2017","journal-title":"Cell. Stem Cell."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib62","doi-asserted-by":"crossref","first-page":"e21296","DOI":"10.1371\/journal.pone.0021296","article-title":"SMA CARNIVAL TRIAL PART II: a prospective, single-armed trial of L-carnitine and valproic acid in ambulatory children with spinal muscular atrophy","volume":"6","author":"Kissel","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib63","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1002\/mus.23904","article-title":"SMA valiant trial: a prospective, double-blind, placebo-controlled trial of valproic acid in ambulatory adults with spinal muscular atrophy","volume":"49","author":"Kissel","year":"2014","journal-title":"Muscle Nerve."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib64","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1177\/2472555216683650","article-title":"Enabling 1536-well high-throughput cell-based screening through the application of novel centrifugal plate washing","volume":"22","author":"Knight","year":"2017","journal-title":"SLAS Discov."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib65","doi-asserted-by":"crossref","first-page":"16647","DOI":"10.1038\/srep16647","article-title":"Long-term maintenance of human induced pluripotent stem cells by automated cell culture system","volume":"5","author":"Konagaya","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib66","first-page":"487","article-title":"Modeling Alzheimer's disease with iPSCs reveals stress phenotypes associated with intracellular A\u03b2 and differential drug responsiveness","volume":"12","author":"Kondo","year":"2013","journal-title":"Stem Cell."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib67","doi-asserted-by":"crossref","first-page":"2304","DOI":"10.1016\/j.celrep.2017.10.109","article-title":"iPSC-based compound screening and in\u00a0vitro trials identify a synergistic anti-amyloid beta combination for Alzheimer's disease","volume":"21","author":"Kondo","year":"2017","journal-title":"Cell Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib68","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.neuint.2018.08.012","article-title":"3D human brain cell models: new frontiers in disease understanding and drug discovery for neurodegenerative diseases","volume":"120","author":"Korhonen","year":"2018","journal-title":"Neurochem. Int."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib69","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1016\/j.stemcr.2017.10.026","article-title":"Systematic three-dimensional coculture rapidly recapitulates interactions between human neurons and astrocytes","volume":"9","author":"Krencik","year":"2017","journal-title":"Stem Cell. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib70","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1038\/nature10648","article-title":"Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson's disease","volume":"480","author":"Kriks","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib71","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1002\/cyto.a.22907","article-title":"Getting the whole picture: high content screening using three-dimensional cellular model systems and whole animal assays","volume":"91","author":"Kriston-Vizi","year":"2017","journal-title":"Cytometry A"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib72","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1039\/c3ib40022k","article-title":"Astrocyte-neuron co-culture on microchips based on the model of SOD mutation to mimic ALS","volume":"5","author":"Kunze","year":"2013","journal-title":"Integr. Biol. (Camb)"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib73","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/978-1-61779-108-6_3","article-title":"Estimation of cell number based on metabolic activity: the MTT reduction assay","volume":"740","author":"Kupcsik","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib74","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1038\/nature08320","article-title":"Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCs","volume":"461","author":"Lee","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib75","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1192\/bjp.bp.110.081513","article-title":"Rapid acute treatment of agitation in individuals with schizophrenia: multicentre, randomised, placebo-controlled study of inhaled loxapine","volume":"198","author":"Lesem","year":"2011","journal-title":"Br. J. Psychiatry"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib76","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.expneurol.2015.07.020","article-title":"Human iPS cell-derived astrocyte transplants preserve respiratory function after spinal cord injury","volume":"271","author":"Li","year":"2015","journal-title":"Exp. Neurol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib77","doi-asserted-by":"crossref","first-page":"9033","DOI":"10.1038\/s41598-018-27058-0","article-title":"A\u00a0single cell high content assay detects mitochondrial dysfunction in iPSC-derived neurons with mutations in SNCA","volume":"8","author":"Little","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib78","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.stem.2016.12.013","article-title":"Human iPSC-derived neural progenitors are an effective drug discovery model for neurological mtDNA disorders","volume":"20","author":"Lorenz","year":"2017","journal-title":"Cell. Stem Cell."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib79","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1126\/science.1259110","article-title":"Cell Biology. Fixing problems with cell lines","volume":"346","author":"Lorsch","year":"2014","journal-title":"Science"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib80","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nbt.3435","article-title":"Generation of serotonin neurons from human pluripotent stem cells","volume":"34","author":"Lu","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib81","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1126\/science.1232033","article-title":"RNA-guided human genome engineering via Cas9","volume":"339","author":"Mali","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib82","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.neuro.2014.10.007","article-title":"Compounds with species and cell type specific toxicity identified in a 2000 compound drug screen of neural stem cells and rat mixed cortical neurons","volume":"45","author":"Malik","year":"2014","journal-title":"Neurotoxicology"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib83","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.cell.2010.10.016","article-title":"A\u00a0model for neural development and treatment of Rett syndrome using human induced pluripotent stem cells","volume":"143","author":"Marchetto","year":"2010","journal-title":"Cell"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib84","first-page":"559","article-title":"Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells","volume":"12","author":"Maroof","year":"2013","journal-title":"Stem Cell."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib85","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.4103\/1673-5374.211180","article-title":"Using induced pluripotent stem cells derived neurons to model brain diseases","volume":"12","author":"McKinney","year":"2017","journal-title":"Neural Regen. Res."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib86","doi-asserted-by":"crossref","first-page":"17209","DOI":"10.1074\/jbc.M109.098814","article-title":"High-throughput screening in embryonic stem cell-derived neurons identifies potentiators of alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate-type glutamate receptors","volume":"285","author":"McNeish","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib87","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1242\/dev.117978","article-title":"Generation of neuropeptidergic hypothalamic neurons from human pluripotent stem cells","volume":"142","author":"Merkle","year":"2015","journal-title":"Development"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib88","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/j.stem.2013.11.006","article-title":"Human iPSC-based modeling of late-onset disease via progerin-induced aging","volume":"13","author":"Miller","year":"2013","journal-title":"Cell. Stem Cell."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib89","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s12975-012-0250-1","article-title":"Differentiation of oligodendrocytes from mouse induced pluripotent stem cells without serum","volume":"4","author":"Misumi","year":"2013","journal-title":"Transl. Stroke Res."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib90","doi-asserted-by":"crossref","first-page":"11573","DOI":"10.1523\/JNEUROSCI.1748-16.2016","article-title":"Compartmentalized microfluidic platforms: the unrivaled breakthrough of in\u00a0vitro tools for neurobiological research","volume":"36","author":"Neto","year":"2016","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib91","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.stem.2011.01.013","article-title":"LRRK2 mutant iPSC-derived DA neurons demonstrate increased susceptibility to oxidative stress","volume":"8","author":"Nguyen","year":"2011","journal-title":"Cell. Stem Cell."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib92","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/j.stem.2013.04.005","article-title":"Functional maturation of hPSC-derived forebrain interneurons requires an extended timeline and mimics human neural development","volume":"12","author":"Nicholas","year":"2013","journal-title":"Cell. Stem Cell."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib93","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1002\/bit.26839","article-title":"High-throughput identification of factors promoting neuronal differentiation of human neural progenitor cells in microscale 3D cell culture","volume":"116","author":"Nierode","year":"2018","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib94","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1002\/glia.20127","article-title":"Human embryonic stem cells differentiate into oligodendrocytes in high purity and myelinate after spinal cord transplantation","volume":"49","author":"Nistor","year":"2005","journal-title":"Glia"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib95","doi-asserted-by":"crossref","first-page":"3837","DOI":"10.1007\/s00018-010-0463-y","article-title":"Human motor neuron generation from embryonic stem cells and induced pluripotent stem cells","volume":"67","author":"Nizzardo","year":"2010","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib96","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ymeth.2017.02.007","article-title":"Analysis of live cell images: methods, tools and opportunities","volume":"115","author":"Nketia","year":"2017","journal-title":"Methods"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib97","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.jpba.2018.07.011","article-title":"Microfluidics contribution to pharmaceutical sciences: from drug discovery to post marketing product management","volume":"159","author":"Nys","year":"2018","journal-title":"J.\u00a0Pharm. Biomed. Anal."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib98","article-title":"Adapting tissue-engineered in\u00a0vitro CNS models for high-throughput study of neurodegeneration","volume":"8","author":"O'Rourke","year":"2017","journal-title":"J.\u00a0Tissue Eng."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib99","doi-asserted-by":"crossref","first-page":"5168","DOI":"10.1038\/s41598-018-23512-1","article-title":"Engineered 3D vascular and neuronal networks in a microfluidic platform","volume":"8","author":"Osaki","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib100","doi-asserted-by":"crossref","first-page":"205","DOI":"10.3727\/000000006783991827","article-title":"Multiregional gene expression profiling identifies MRPS6 as a possible candidate gene for Parkinson's disease","volume":"13","author":"Papapetropoulos","year":"2006","journal-title":"Gene Expr."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib101","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1038\/nature25032","article-title":"The rise of three-dimensional human brain cultures","volume":"553","author":"Pasca","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib102","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1038\/cr.2011.133","article-title":"Defining the nature of human pluripotent stem cell progeny","volume":"22","author":"Patterson","year":"2012","journal-title":"Cell Res."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib103","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1038\/nmeth.3507","article-title":"Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells","volume":"12","author":"Paull","year":"2015","journal-title":"Nat. Methods"},{"issue":"Pt A","key":"10.1016\/j.neurobiolaging.2019.02.008_bib104","first-page":"57","article-title":"Comparative neurotoxicity screening in human iPSC-derived neural stem cells, neurons and astrocytes","volume":"1638","author":"Pei","year":"2015","journal-title":"Brain Res."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib105","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1177\/1087057112474468","article-title":"Using human pluripotent stem cell-derived dopaminergic neurons to evaluate candidate Parkinson's disease therapeutic agents in MPP+ and rotenone models","volume":"18","author":"Peng","year":"2013","journal-title":"J.\u00a0Biomol. Screen."},{"issue":"Pt B","key":"10.1016\/j.neurobiolaging.2019.02.008_bib106","first-page":"190","article-title":"Modeling neurodegenerative diseases with patient-derived induced pluripotent cells: possibilities and challenges","volume":"39","author":"Poon","year":"2017","journal-title":"N.\u00a0Biotechnol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib107","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1111\/nan.12334","article-title":"Review: induced pluripotent stem cell models of frontotemporal dementia","volume":"42","author":"Preza","year":"2016","journal-title":"Neuropathol. Appl. Neurobiol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib108","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/nbt.3777","article-title":"Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells","volume":"35","author":"Qi","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib109","doi-asserted-by":"crossref","DOI":"10.3390\/ht7020018","article-title":"Microfluidic devices for drug assays","volume":"7","author":"Regnault","year":"2018","journal-title":"High Throughput."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib110","doi-asserted-by":"crossref","first-page":"e59252","DOI":"10.1371\/journal.pone.0059252","article-title":"Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling","volume":"8","author":"Reinhardt","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib111","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1089\/adt.2006.4.143","article-title":"A\u00a0morphology- and kinetics-based cascade for human neural cell high content screening","volume":"4","author":"Richards","year":"2006","journal-title":"Assay Drug Dev. Technol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib112","doi-asserted-by":"crossref","first-page":"2587","DOI":"10.1017\/S0033291717002124","article-title":"Human induced pluripotent stem cells as a research tool in Alzheimer's disease","volume":"47","author":"Robbins","year":"2017","journal-title":"Psychol. Med."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib113","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/j.1748-720X.2010.00479.x","article-title":"Embryo stem cell research: ten years of controversy","volume":"38","author":"Robertson","year":"2010","journal-title":"J.\u00a0Law Med. Ethics"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib114","doi-asserted-by":"crossref","first-page":"2354","DOI":"10.1038\/nprot.2014.159","article-title":"Monolayer culturing and cloning of human pluripotent stem cells on laminin-521-based matrices under xeno-free and chemically defined conditions","volume":"9","author":"Rodin","year":"2014","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib115","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1016\/j.cell.2013.11.009","article-title":"Isogenic human iPSC Parkinson's model shows nitrosative stress-induced dysfunction in MEF2-PGC1alpha transcription","volume":"155","author":"Ryan","year":"2013","journal-title":"Cell"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib116","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.neuro.2016.02.003","article-title":"Neurite outgrowth in human induced pluripotent stem cell-derived neurons as a high-throughput screen for developmental neurotoxicity or neurotoxicity","volume":"53","author":"Ryan","year":"2016","journal-title":"Neurotoxicology"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib117","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1002\/emmm.201200215","article-title":"Disease-specific phenotypes in dopamine neurons from human iPS-based models of genetic and sporadic Parkinson's disease","volume":"4","author":"Sanchez-Danes","year":"2012","journal-title":"EMBO Mol. Med."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib118","doi-asserted-by":"crossref","DOI":"10.3390\/ijms18040888","article-title":"Induced pluripotent stem cell modeling of Gaucher's disease: what have we learned?","volume":"18","author":"Santos","year":"2017","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib119","doi-asserted-by":"crossref","first-page":"4028","DOI":"10.1038\/ncomms5028","article-title":"iPSC-derived neurons from GBA1-associated Parkinson's disease patients show autophagic defects and impaired calcium homeostasis","volume":"5","author":"Schondorf","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib120","doi-asserted-by":"crossref","DOI":"10.1242\/dmm.031906","article-title":"High-throughput screen for compounds that modulate neurite growth of human induced pluripotent stem cell-derived neurons","volume":"11","author":"Sherman","year":"2018","journal-title":"Dis. Model Mech."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib121","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1038\/nprot.2012.116","article-title":"Directed differentiation of human pluripotent stem cells to cerebral cortex neurons and neural networks","volume":"7","author":"Shi","year":"2012","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib122","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1111\/bpa.12526","article-title":"Modeling Parkinson's disease with induced pluripotent stem cells harboring alpha-synuclein mutations","volume":"27","author":"Singh Dolt","year":"2017","journal-title":"Brain Pathol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib123","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1093\/toxsci\/kfy218","article-title":"Functional and mechanistic neurotoxicity profiling using human iPSC-derived neural 3D cultures","volume":"167","author":"Sirenko","year":"2018","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib124","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1111\/bpa.12517","article-title":"Understanding neurodevelopmental disorders using human pluripotent stem cell-derived neurons","volume":"27","author":"Tamburini","year":"2017","journal-title":"Brain Pathol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib125","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/j.scr.2013.05.002","article-title":"Astroglial cells regulate the developmental timeline of human neurons differentiated from induced pluripotent stem cells","volume":"11","author":"Tang","year":"2013","journal-title":"Stem Cell. Res."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib126","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/s41551-018-0219-9","article-title":"Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons","volume":"2","author":"Tekin","year":"2018","journal-title":"Nat. Biomed. Eng."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib127","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1243\/09544119JEIM375","article-title":"Automated surgical screwdriver: automated screw placement","volume":"222","author":"Thomas","year":"2008","journal-title":"Proc. Inst. Mech. Eng. H"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib128","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.nbd.2015.06.011","article-title":"Modeling amyotrophic lateral sclerosis in pure human iPSc-derived motor neurons isolated by a novel FACS double selection technique","volume":"82","author":"Toli","year":"2015","journal-title":"Neurobiol. Dis."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib129","doi-asserted-by":"crossref","first-page":"99","DOI":"10.3233\/JHD-160199","article-title":"Induced pluripotent stem cells in Huntington's disease research: progress and opportunity","volume":"5","author":"Tousley","year":"2016","journal-title":"J.\u00a0Huntingtons Dis."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib130","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/j.stemcr.2017.06.008","article-title":"Reactive astrocytes promote ALS-like degeneration and intracellular protein aggregation in human motor neurons by disrupting autophagy through TGF-beta1","volume":"9","author":"Tripathi","year":"2017","journal-title":"Stem Cell. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib131","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.scr.2015.05.002","article-title":"A\u00a0chemically defined substrate for the expansion and neuronal differentiation of human pluripotent stem cell-derived neural progenitor cells","volume":"15","author":"Tsai","year":"2015","journal-title":"Stem Cell. Res."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib132","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/mp.2015.161","article-title":"Generation of functional human serotonergic neurons from fibroblasts","volume":"21","author":"Vadodaria","year":"2016","journal-title":"Mol. Psychiatry"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib133","doi-asserted-by":"crossref","first-page":"e6722","DOI":"10.1371\/journal.pone.0006722","article-title":"Highly efficient differentiation and enrichment of spinal motor neurons derived from human and monkey embryonic stem cells","volume":"4","author":"Wada","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib134","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/nrd1063","article-title":"Designing screens: how to make your hits a hit","volume":"2","author":"Walters","year":"2003","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib135","doi-asserted-by":"crossref","first-page":"9232","DOI":"10.1038\/srep09232","article-title":"Differentiation of human induced pluripotent stem cells to mature functional Purkinje neurons","volume":"5","author":"Wang","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib136","doi-asserted-by":"crossref","first-page":"471","DOI":"10.2217\/pgs-2016-0187","article-title":"Stem cell-derived neurons in the development of targeted treatment for schizophrenia and bipolar disorder","volume":"18","author":"Watmuff","year":"2017","journal-title":"Pharmacogenomics"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib137","series-title":"Neurological Disorders: Public Health Challenges","author":"WHO","year":"2006"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib138","doi-asserted-by":"crossref","first-page":"20270","DOI":"10.1038\/srep20270","article-title":"Transcriptional comparison of human induced and primary midbrain dopaminergic neurons","volume":"6","author":"Xia","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib139","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.scr.2012.11.005","article-title":"Prevention of \u03b2-amyloid induced toxicity in human iPS cell-derived neurons by inhibition of Cyclin-dependent kinases and associated cell cycle events","volume":"10","author":"Xu","year":"2013","journal-title":"Stem Cell. Res."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib140","doi-asserted-by":"crossref","first-page":"4530","DOI":"10.1093\/hmg\/ddr394","article-title":"Modeling familial Alzheimer's disease with induced pluripotent stem cells","volume":"20","author":"Yagi","year":"2011","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib141","first-page":"713","article-title":"A\u00a0small molecule screen in stem-cell-derived motor neurons identifies a kinase inhibitor as a candidate therapeutic for ALS","volume":"12","author":"Yang","year":"2013","journal-title":"Stem Cell."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib142","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/nmeth.4291","article-title":"Generation of pure GABAergic neurons by transcription factor programming","volume":"14","author":"Yang","year":"2017","journal-title":"Nat. Methods"},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib143","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/nchembio.1809","article-title":"Application guide for omics approaches to cell signaling","volume":"11","author":"Yao","year":"2015","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib144","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.stemcr.2014.01.009","article-title":"Modeling hippocampal neurogenesis using human pluripotent stem cells","volume":"2","author":"Yu","year":"2014","journal-title":"Stem Cell. Rep."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib145","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.tibtech.2017.04.002","article-title":"Optogenetic approaches to drug discovery in neuroscience and beyond","volume":"35","author":"Zhang","year":"2017","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.neurobiolaging.2019.02.008_bib146","doi-asserted-by":"crossref","first-page":"239","DOI":"10.3389\/fncel.2018.00239","article-title":"Differentiation of glial cells from hiPSCs: potential applications in neurological diseases and cell replacement therapy","volume":"12","author":"Zheng","year":"2018","journal-title":"Front. Cell. Neurosci."}],"container-title":["Neurobiology of Aging"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0197458019300557?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0197458019300557?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T11:29:23Z","timestamp":1759318163000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0197458019300557"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6]]},"references-count":146,"alternative-id":["S0197458019300557"],"URL":"https:\/\/doi.org\/10.1016\/j.neurobiolaging.2019.02.008","relation":{},"ISSN":["0197-4580"],"issn-type":[{"value":"0197-4580","type":"print"}],"subject":[],"published":{"date-parts":[[2019,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Using stem cell\u2013derived neurons in drug screening for neurological diseases","name":"articletitle","label":"Article Title"},{"value":"Neurobiology of Aging","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.neurobiolaging.2019.02.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}