{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:47:35Z","timestamp":1790477255078,"version":"4.1.0"},"reference-count":38,"publisher":"Elsevier BV","issue":"7-8","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,9,10]],"date-time":"2020-09-10T00:00:00Z","timestamp":1599696000000},"content-version":"am","delay-in-days":375,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Revue Neurologique"],"published-print":{"date-parts":[[2019,9]]},"DOI":"10.1016\/j.neurol.2019.06.002","type":"journal-article","created":{"date-parts":[[2019,7,8]],"date-time":"2019-07-08T07:39:09Z","timestamp":1562571549000},"page":"458-463","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"title":["Amplification techniques and diagnosis of prion diseases"],"prefix":"10.1016","volume":"175","author":[{"given":"J.-P.","family":"Brandel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Culeux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Grznarova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Levavasseur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Lamy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Privat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Welaratne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Denouel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.-L.","family":"Laplanche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Haik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.neurol.2019.06.002_bib0195","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1126\/science.6801762","article-title":"Novel proteinaceous infectious particles cause scrapie","volume":"216","author":"Prusiner","year":"1982","journal-title":"Science"},{"key":"10.1016\/j.neurol.2019.06.002_bib0200","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1002\/ana.20261","article-title":"Diagnostic value of periodic complexes in Creutzfeldt\u2013Jakob disease","volume":"56","author":"Steinhoff","year":"2004","journal-title":"Ann Neurol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0205","doi-asserted-by":"crossref","first-page":"1994","DOI":"10.1212\/WNL.0b013e3181a96e5d","article-title":"MRI lesion profiles in sporadic Creutzfeldt\u2013Jakob disease","volume":"72","author":"Meissner","year":"2009","journal-title":"Neurology"},{"key":"10.1016\/j.neurol.2019.06.002_bib0210","first-page":"1459","article-title":"Creutzfeldt\u2013Jakob disease: comparative analysis of MR imaging sequences","volume":"27","author":"Kallenberg","year":"2006","journal-title":"AJNR Am J Neuroradiol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0215","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1016\/S0140-6736(00)02140-1","article-title":"The pulvinar sign on magnetic resonance imaging in variant Creutzfeldt\u2013Jakob disease","volume":"355","author":"Zeidler","year":"2000","journal-title":"Lancet"},{"key":"10.1016\/j.neurol.2019.06.002_bib0220","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1212\/WNL.0b013e31826d5fc3","article-title":"Evidence-based guideline: diagnostic accuracy of CSF 14-3-3 protein in sporadic Creutzfeldt- Jakob disease: report of the guideline development subcommittee of the American Academy of Neurology","volume":"79","author":"Muayqil","year":"2012","journal-title":"Neurology"},{"key":"10.1016\/j.neurol.2019.06.002_bib0225","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1002\/1531-8249(20010201)49:2<257::AID-ANA48>3.0.CO;2-X","article-title":"14-3-3 protein cerebrospinal fluid detection in human growth hormone-treated Creutzfeldt\u2013Jakob disease patients","volume":"49","author":"Brandel","year":"2001","journal-title":"Ann Neurol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0230","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1007\/s00439-005-0020-1","article-title":"EUROCJD. Genetic prion disease: the EUROCJD experience","volume":"118","author":"Kov\u00e1cs","year":"2005","journal-title":"Hum Genet"},{"key":"10.1016\/j.neurol.2019.06.002_bib0235","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1136\/jnnp.70.6.744","article-title":"Use of 14-3-3 and other brain-specific proteins in CSF in the diagnosis of variant Creutzfeldt\u2013Jakob disease","volume":"70","author":"Green","year":"2001","journal-title":"J Neurol Neurosurg Psychiatry"},{"key":"10.1016\/j.neurol.2019.06.002_bib0240","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1002\/ana.21583","article-title":"Variant Creutzfeldt\u2013Jakob disease in France and the United Kingdom: evidence for the same agent strain","volume":"65","author":"Brandel","year":"2009","journal-title":"Ann Neurol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0245","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1136\/jnnp.2009.197962","article-title":"The role of cerebrospinal fluid 14-3-3 and other proteins in the diagnosis of sporadic Creutzfeldt\u2013Jakob disease in the UK: a 10-year review","volume":"81","author":"Chohan","year":"2010","journal-title":"J Neurol Neurosurg Psychiatry"},{"key":"10.1016\/j.neurol.2019.06.002_bib0250","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/S0166-2236(02)02195-1","article-title":"Cyclic amplification of protein misfolding: application to prion-related disorders and beyond","volume":"25","author":"Soto","year":"2002","journal-title":"Trends Neurosci"},{"key":"10.1016\/j.neurol.2019.06.002_bib0255","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1038\/nmeth1066","article-title":"Ultrasensitive detection of scrapie prion protein using seeded conversion of recombinant prion protein","volume":"4","author":"Atarashi","year":"2007","journal-title":"Nat Methods"},{"key":"10.1016\/j.neurol.2019.06.002_bib0260","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1038\/35081095","article-title":"Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding","volume":"411","author":"Saborio","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.neurol.2019.06.002_bib0265","doi-asserted-by":"crossref","first-page":"e66352","DOI":"10.1371\/journal.pone.0066352","article-title":"Re-assessment of PrP (Sc) distribution in sporadic and variant CJD","volume":"8","author":"Rubenstein","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.neurol.2019.06.002_bib0270","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1056\/NEJMoa1404401","article-title":"Prions in the urine of patients with variant Creutzfeldt\u2013Jakob disease","volume":"371","author":"Moda","year":"2014","journal-title":"N Engl J Med"},{"key":"10.1016\/j.neurol.2019.06.002_bib0275","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1126\/scitranslmed.aag1257","article-title":"Detection of prions in the plasma of presymptomatic and symptomatic patients with variant Creutzfeldt\u2013Jakob disease","volume":"8","author":"Bougard","year":"2016","journal-title":"Sci Transl Med"},{"key":"10.1016\/j.neurol.2019.06.002_bib0280","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1126\/scitranslmed.aaf6188","article-title":"Detection of prions in blood from patients with variant Creutzfeldt\u2013Jakob disease","volume":"8","author":"Concha-Marambio","year":"2016","journal-title":"Sci Transl Med"},{"key":"10.1016\/j.neurol.2019.06.002_bib0285","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.3201\/eid2407.172105","article-title":"Diagnosis of Methionine\/Valine Variant Creutzfeldt-Jakob Disease by Protein Misfolding Cyclic Amplification","volume":"24","author":"Bougard","year":"2018","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.neurol.2019.06.002_bib0290","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.neulet.2011.04.072","article-title":"Heparin enhances the cell-protein misfolding cyclic amplification efficiency of variant Creutzfeldt-Jakob disease","volume":"498","author":"Yokoyama","year":"2011","journal-title":"Neurosci Lett"},{"key":"10.1016\/j.neurol.2019.06.002_bib0295","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1038\/nm1286","article-title":"Detection of prions in blood","volume":"11","author":"Castilla","year":"2005","journal-title":"Nat Med"},{"key":"10.1016\/j.neurol.2019.06.002_bib0300","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.vetmic.2007.04.004","article-title":"Generation of genuine prion infectivity by serial PMCA","volume":"123","author":"Weber","year":"2007","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0305","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/j.cell.2008.07.030","article-title":"Crossing the species barrier by PrP (Sc) replication in vitro generates unique infectious prions","volume":"134","author":"Castilla","year":"2008","journal-title":"Cell"},{"key":"10.1016\/j.neurol.2019.06.002_bib0310","doi-asserted-by":"crossref","first-page":"e1001217","DOI":"10.1371\/journal.ppat.1001217","article-title":"Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays","volume":"6","author":"Wilham","year":"2010","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.neurol.2019.06.002_bib0315","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1038\/nm.2294","article-title":"Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion","volume":"17","author":"Atarashi","year":"2011","journal-title":"Nat Med"},{"issue":"1","key":"10.1016\/j.neurol.2019.06.002_bib0320","doi-asserted-by":"crossref","first-page":"e02451","DOI":"10.1128\/mBio.02451-14","article-title":"Rapid and sensitive RT QuIC detection of human Creutzfeldt\u2013Jakob disease using cerebrospinal fluid","volume":"6","author":"Orru","year":"2015","journal-title":"MBio"},{"key":"10.1016\/j.neurol.2019.06.002_bib0325","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1002\/path.2204","article-title":"In vitro amplification and detection of variant Creutzfeldt-Jakob disease PrPSc","volume":"213","author":"Jones","year":"2007","journal-title":"J Pathol."},{"key":"10.1016\/j.neurol.2019.06.002_bib0330","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1002\/ana.23589","article-title":"Real time quaking-induced conversion analysis of cerebrospinal fluid in sporadic Creutzfeldt\u2013Jakob disease","volume":"72","author":"McGuire","year":"2012","journal-title":"Ann Neurol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0335","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.1007\/s12035-015-9133-2","article-title":"Stability and reproducibility underscore utility of RT-QuIC for diagnosis of Creutzfeldt\u2013Jakob disease","volume":"53","author":"Cramm","year":"2016","journal-title":"Mol Neurobiol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0340","doi-asserted-by":"crossref","first-page":"101","DOI":"10.3988\/jcn.2016.12.1.101","article-title":"Real-Time quaking-induced conversion analysis for the diagnosis of sporadic Creutzfeldt\u2013Jakob disease in Korea","volume":"12","author":"Park","year":"2016","journal-title":"J Clin Neurol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0345","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1002\/acn3.378","article-title":"Extended and direct evaluation of RT-QuIC assays for Creutzfeldt\u2013Jakob disease diagnosis","volume":"4","author":"Groveman","year":"2017","journal-title":"Ann Clin Transl Neurol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0350","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1007\/s00401-017-1683-0","article-title":"Prion-specific and surrogate CSF biomarkers in Creutzfeldt-Jakob disease: diagnostic accuracy in relation to molecular subtypes and analysis of neuropathological correlates of p-tau and A\u03b242 levels","volume":"133","author":"Lattanzio","year":"2017","journal-title":"Acta Neuropathol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0355","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1002\/ana.24833","article-title":"Diagnostic and prognostic value of human prion detection in cerebrospinal fluid","volume":"81","author":"Foutz","year":"2017","journal-title":"Ann Neurol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0360","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1056\/NEJMoa1315200","article-title":"A test for Creutzfeldt-Jakob disease using nasal brushings","volume":"371","author":"Orr\u00fa","year":"2014","journal-title":"N Engl J Med"},{"issue":"417.","key":"10.1016\/j.neurol.2019.06.002_bib0365","article-title":"Prion seeding activity and infectivity in skin samples from patients with sporadic Creutzfeldt-Jakob disease","volume":"9","author":"Orr\u00fa","year":"2017","journal-title":"Sci Transl Med"},{"key":"10.1016\/j.neurol.2019.06.002_bib0370","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/B978-0-444-63945-5.00019-2","article-title":"Prion protein amplification techniques","volume":"153","author":"Green","year":"2018","journal-title":"Handb Clin Neurol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0375","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1001\/jamaneurol.2016.4614","article-title":"Diagnosis of human prion disease using real-time quaking-induced conversion testing of olfactory mucosa and cerebrospinal fluid samples","volume":"74","author":"Bongianni","year":"2017","journal-title":"JAMA Neurol"},{"key":"10.1016\/j.neurol.2019.06.002_bib0380","doi-asserted-by":"crossref","first-page":"10655","DOI":"10.1038\/s41598-017-10922-w","article-title":"High diagnostic value of second generation CSF RT-QuIC across the wide spectrum of CJD prions","volume":"7","author":"Franceschini","year":"2017","journal-title":"Sci Rep"}],"container-title":["Revue Neurologique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0035378719306575?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0035378719306575?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T05:39:54Z","timestamp":1758519594000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0035378719306575"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":38,"journal-issue":{"issue":"7-8","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["S0035378719306575"],"URL":"https:\/\/doi.org\/10.1016\/j.neurol.2019.06.002","relation":{},"ISSN":["0035-3787"],"issn-type":[{"value":"0035-3787","type":"print"}],"subject":[],"published":{"date-parts":[[2019,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Amplification techniques and diagnosis of prion diseases","name":"articletitle","label":"Article Title"},{"value":"Revue Neurologique","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.neurol.2019.06.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Masson SAS. All rights reserved.","name":"copyright","label":"Copyright"}]}}