{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T03:15:16Z","timestamp":1773890116020,"version":"3.50.1"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100005693","name":"Hainan University","doi-asserted-by":"publisher","award":["202510"],"award-info":[{"award-number":["202510"]}],"id":[{"id":"10.13039\/501100005693","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008111","name":"Hainan Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["GHYF2023003"],"award-info":[{"award-number":["GHYF2023003"]}],"id":[{"id":"10.13039\/501100008111","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008111","name":"Hainan Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["WSJK2024MS228"],"award-info":[{"award-number":["WSJK2024MS228"]}],"id":[{"id":"10.13039\/501100008111","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22578089"],"award-info":[{"award-number":["22578089"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22268018"],"award-info":[{"award-number":["22268018"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22508074"],"award-info":[{"award-number":["22508074"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Nitric Oxide"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.niox.2025.11.007","type":"journal-article","created":{"date-parts":[[2025,11,23]],"date-time":"2025-11-23T07:06:58Z","timestamp":1763881618000},"page":"47-55","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Rapid fabrication of nitric oxide releasing hydroxyapatite microspheres using liquid nitrogen as an antibiotic-free alternative"],"prefix":"10.1016","volume":"160","author":[{"given":"Peixuan","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhendong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeyu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingle","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tawfik","family":"Khattab","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1934-102X","authenticated-orcid":false,"given":"Yuanyuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.niox.2025.11.007_bib1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/s41779-020-00514-9","article-title":"Batch preparation of homogeneous size hydroxyapatite based composite ceramic microspheres","volume":"57","author":"Zhou","year":"2021","journal-title":"Journal of the Australian Ceramic Society"},{"key":"10.1016\/j.niox.2025.11.007_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmbbm.2024.106661","article-title":"Dual-functional Hydroxyapatite scaffolds for bone regeneration and precision drug delivery","volume":"157","author":"Farazin","year":"2024","journal-title":"J. Mech. Behav. Biomed. Mater."},{"issue":"7","key":"10.1016\/j.niox.2025.11.007_bib3","doi-asserted-by":"crossref","first-page":"11369","DOI":"10.1016\/j.ceramint.2014.02.107","article-title":"Zinc alginate\u2013hydroxyapatite composite microspheres for bone repair","volume":"40","author":"Costa Cuozzo","year":"2014","journal-title":"Ceram. Int."},{"key":"10.1016\/j.niox.2025.11.007_bib4","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.matlet.2017.02.056","article-title":"Controlled synthesis of hydroxyapatite microspheres with hierarchical structure and high cell viability","volume":"195","author":"Zheng","year":"2017","journal-title":"Mater. Lett."},{"issue":"2","key":"10.1016\/j.niox.2025.11.007_bib5","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1046\/j.1525-1594.2003.06953.x","article-title":"Collagen-hydroxyapatite microspheres as carriers for bone morphogenic protein-4","volume":"27","author":"Wang","year":"2003","journal-title":"Artif. Organs"},{"issue":"4","key":"10.1016\/j.niox.2025.11.007_bib6","article-title":"Light-controlled smart materials: supramolecular regulation and applications","volume":"2","author":"Liao","year":"2024","journal-title":"Smart Mol."},{"issue":"1","key":"10.1016\/j.niox.2025.11.007_bib7","article-title":"Smart molecules in ophthalmology: hydrogels as responsive systems for ophthalmic applications","volume":"2","author":"Kulbay","year":"2024","journal-title":"Smart Mol."},{"issue":"3","key":"10.1016\/j.niox.2025.11.007_bib8","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0144-8617(01)00331-9","article-title":"Preparation, characterization and in-vitro release of gentamicin from coralline hydroxyapatite\u2013chitosan composite microspheres","volume":"49","author":"Sivakumar","year":"2002","journal-title":"Carbohydr. Polym."},{"key":"10.1016\/j.niox.2025.11.007_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.cis.2023.103013","article-title":"Hydroxyapatite: a journey from biomaterials to advanced functional materials","volume":"321","author":"Mondal","year":"2023","journal-title":"Adv. Colloid Interface Sci."},{"key":"10.1016\/j.niox.2025.11.007_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.porgcoat.2020.106056","article-title":"Synthesis, biological and environmental applications of hydroxyapatite and its composites with organic and inorganic coatings","volume":"151","author":"Varadavenkatesan","year":"2021","journal-title":"Prog. Org. Coating"},{"key":"10.1016\/j.niox.2025.11.007_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.mser.2024.100870","article-title":"Exploring the potential of hydroxyapatite-based materials in biomedicine: a comprehensive review","volume":"161","author":"Liu","year":"2024","journal-title":"Mater. Sci. Eng. R Rep."},{"issue":"1","key":"10.1016\/j.niox.2025.11.007_bib12","article-title":"Photodynamic and photothermal therapies for bacterial infection treatment","volume":"1","author":"Kim","year":"2023","journal-title":"Smart Mol."},{"issue":"1","key":"10.1016\/j.niox.2025.11.007_bib13","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-76088-4","article-title":"Enhanced antimicrobial efficacy of hydroxyapatite-based composites for healthcare applications","volume":"14","author":"Hassanain","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.niox.2025.11.007_bib14","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.msec.2018.04.076","article-title":"Synthesis of monophasic Ag doped hydroxyapatite and evaluation of antibacterial activity","volume":"90","author":"Riaz","year":"2018","journal-title":"Mater. Sci. Eng. C"},{"key":"10.1016\/j.niox.2025.11.007_bib15","article-title":"A review on the antimicrobial and antibiofilm activity of doped hydroxyapatite and its composites for biomedical applications","volume":"31","author":"Ghosh","year":"2022","journal-title":"Mater. Today Commun."},{"issue":"1","key":"10.1016\/j.niox.2025.11.007_bib16","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-80303-7","article-title":"Effect of silver ion and silicate group on the antibacterial and antifungal properties of nanosized hydroxyapatite","volume":"14","author":"Pinchuk","year":"2024","journal-title":"Sci. Rep."},{"issue":"4","key":"10.1016\/j.niox.2025.11.007_bib17","first-page":"113","article-title":"Synthesis and antibacterial property of silver doped hydroxyapatite by precipitation and hydrothermal method","volume":"45","author":"Cheng","year":"2017","journal-title":"J. Mater. Eng."},{"key":"10.1016\/j.niox.2025.11.007_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2021.130133","article-title":"Polymer brush grafted antimicrobial peptide on hydroxyapatite nanorods for highly effective antibacterial performance","volume":"423","author":"Li","year":"2021","journal-title":"Chem. Eng. J."},{"issue":"9","key":"10.1016\/j.niox.2025.11.007_bib19","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0075846","article-title":"Platelet activating factor-induced ceramide micro-domains drive endothelial NOS activation and contribute to barrier dysfunction","volume":"8","author":"Predescu","year":"2013","journal-title":"PLoS One"},{"issue":"7","key":"10.1016\/j.niox.2025.11.007_bib20","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0130286","article-title":"Clinical implications of iNOS levels in triple-negative breast cancer responding to neoadjuvant chemotherapy","volume":"10","author":"Jin","year":"2015","journal-title":"PLoS One"},{"issue":"3","key":"10.1016\/j.niox.2025.11.007_bib21","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.tplants.2010.11.007","article-title":"On the origins of nitric oxide","volume":"16","author":"Gupta","year":"2011","journal-title":"Trends Plant Sci."},{"issue":"3","key":"10.1016\/j.niox.2025.11.007_bib22","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/j.1745-7254.2005.00058.x","article-title":"Nitric oxide: a newly discovered function on wound healing","volume":"26","author":"Luo","year":"2005","journal-title":"Acta Pharmacol. Sin."},{"key":"10.1016\/j.niox.2025.11.007_bib23","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.redox.2013.12.027","article-title":"Endothelial nitric oxide synthase in red blood cells: key to a new erythrocrine function?","volume":"2","author":"Cortese-Krott","year":"2014","journal-title":"Redox Biol."},{"issue":"5","key":"10.1016\/j.niox.2025.11.007_bib24","doi-asserted-by":"crossref","first-page":"2993","DOI":"10.1021\/acsabm.4c00051","article-title":"Engineering nitric oxide-releasing antimicrobial dental coating for targeted gingival therapy","volume":"7","author":"Chug","year":"2024","journal-title":"ACS Appl. Bio Mater."},{"issue":"2","key":"10.1016\/j.niox.2025.11.007_bib25","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1021\/acsmaterialsau.1c00056","article-title":"Antimicrobial nitric oxide-releasing electrospun dressings for wound healing applications","volume":"2","author":"Li","year":"2022","journal-title":"ACS Mater. Au"},{"key":"10.1016\/j.niox.2025.11.007_bib26","article-title":"A photothermal responsive system accelerating nitric oxide release to enhance bone repair by promoting osteogenesis and angiogenesis","volume":"28","author":"Cheng","year":"2024","journal-title":"Mater. Today Bio"},{"key":"10.1016\/j.niox.2025.11.007_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2024.151566","article-title":"Nickel nitride-mediated nitric oxide generation for combating implant-associated infections","volume":"490","author":"Wei","year":"2024","journal-title":"Chem. Eng. J."},{"issue":"10","key":"10.1016\/j.niox.2025.11.007_bib28","doi-asserted-by":"crossref","first-page":"2950","DOI":"10.1021\/mp300139y","article-title":"Liposome encapsulation of a photochemical NO precursor for controlled nitric oxide release and simultaneous fluorescence imaging","volume":"9","author":"Ostrowski","year":"2012","journal-title":"Mol. Pharm."},{"issue":"13","key":"10.1016\/j.niox.2025.11.007_bib29","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.201800155","article-title":"Nitric oxide-releasing macromolecular scaffolds for antibacterial applications","volume":"7","author":"Yang","year":"2018","journal-title":"Adv. Healthcare Mater."},{"issue":"3","key":"10.1016\/j.niox.2025.11.007_bib30","doi-asserted-by":"crossref","first-page":"217","DOI":"10.4161\/viru.2.3.16161","article-title":"Susceptibility of Gram-positive and -negative bacteria to novel nitric oxide-releasing nanoparticle technology","volume":"2","author":"Friedman","year":"2011","journal-title":"Virulence"},{"key":"10.1016\/j.niox.2025.11.007_bib31","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1016\/j.ijbiomac.2022.10.210","article-title":"Nitric oxide releasing alginate microspheres for antimicrobial application","volume":"224","author":"Zhuang","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"issue":"4","key":"10.1016\/j.niox.2025.11.007_bib32","article-title":"Metallo-supramolecular nanofibers based on type-I photosensitizer for synergistic antibacterial therapy","volume":"2","author":"Lyu","year":"2024","journal-title":"Smart Mol."},{"issue":"n\/a","key":"10.1016\/j.niox.2025.11.007_bib33","article-title":"A mitochondria-targeted aggregation-induced emission photosensitizer for eradication Candida biofilms and treating oral ulcer","author":"Liu","year":"2025","journal-title":"Smart Mol."},{"key":"10.1016\/j.niox.2025.11.007_bib34","doi-asserted-by":"crossref","DOI":"10.1002\/smo.20240067","article-title":"Microneedle delivery systems for vaccines and immunotherapy","author":"Jia","year":"2025","journal-title":"Smart Mol."},{"issue":"n\/a","key":"10.1016\/j.niox.2025.11.007_bib35","article-title":"Acute kidney injury and iron metabolism: a narrative review focusing on pathophysiology and therapy","author":"Tan","year":"2025","journal-title":"Smart Mol."},{"issue":"10","key":"10.1016\/j.niox.2025.11.007_bib36","doi-asserted-by":"crossref","first-page":"3731","DOI":"10.1039\/c2cs15272j","article-title":"Nitric oxide release: part I. Macromolecular scaffolds","volume":"41","author":"Riccio","year":"2012","journal-title":"Chem. Soc. Rev."},{"issue":"24","key":"10.1016\/j.niox.2025.11.007_bib37","doi-asserted-by":"crossref","first-page":"2579","DOI":"10.2174\/0929867323666160729104647","article-title":"Nitric oxide-releasing biomaterials for biomedical applications","volume":"23","author":"Zhou","year":"2016","journal-title":"Curr. Med. Chem."},{"key":"10.1016\/j.niox.2025.11.007_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.polymer.2021.123943","article-title":"Delivering nitric oxide with poly(n-butyl methacrylate) films doped with S-nitroso-N-acetylpenicillamine","volume":"228","author":"Zhou","year":"2021","journal-title":"Polymer"},{"key":"10.1016\/j.niox.2025.11.007_bib39","article-title":"Synthesis, spectroscopy, and stability of the nitric oxide donor S-nitroso-N-acetylpenicillamine: an undergraduate laboratory experiment","author":"Ketchum","year":"2017","journal-title":"Journal of Laboratory Chemical Education"},{"issue":"7","key":"10.1016\/j.niox.2025.11.007_bib40","doi-asserted-by":"crossref","first-page":"7727","DOI":"10.1016\/j.ceramint.2013.03.029","article-title":"Characterization by Fourier transform infrared spectroscopy of hydroxyapatite co-doped with zinc and fluoride","volume":"39","author":"Uysal","year":"2013","journal-title":"Ceram. Int."},{"issue":"2","key":"10.1016\/j.niox.2025.11.007_bib41","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.actbio.2009.07.001","article-title":"In situ generation of sodium alginate\/hydroxyapatite nanocomposite beads as drug-controlled release matrices","volume":"6","author":"Zhang","year":"2010","journal-title":"Acta Biomater."},{"issue":"28","key":"10.1016\/j.niox.2025.11.007_bib42","doi-asserted-by":"crossref","first-page":"6805","DOI":"10.1039\/D4TB00551A","article-title":"Surface functionalization of hydroxyapatite nanoparticles for biomedical applications","volume":"12","author":"Kataoka","year":"2024","journal-title":"J. Mater. Chem. B"}],"container-title":["Nitric Oxide"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1089860325001028?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1089860325001028?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T23:48:05Z","timestamp":1773877685000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1089860325001028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":42,"alternative-id":["S1089860325001028"],"URL":"https:\/\/doi.org\/10.1016\/j.niox.2025.11.007","relation":{},"ISSN":["1089-8603"],"issn-type":[{"value":"1089-8603","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rapid fabrication of nitric oxide releasing hydroxyapatite microspheres using liquid nitrogen as an antibiotic-free alternative","name":"articletitle","label":"Article Title"},{"value":"Nitric Oxide","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.niox.2025.11.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}