{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T22:36:07Z","timestamp":1773959767448,"version":"3.50.1"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:00:00Z","timestamp":1767139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006458","name":"The Children's Hospital of Philadelphia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006458","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Nitric Oxide"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1016\/j.niox.2025.12.002","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T07:21:26Z","timestamp":1766992886000},"page":"1-8","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Analytic performance characteristics of the chemical-reduction gas phase-chemiluminescence assay for the quantification of nitric oxide metabolites"],"prefix":"10.1016","volume":"161","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8588-4834","authenticated-orcid":false,"given":"Michelle Menon","family":"Miyake","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ipsita","family":"Mohanty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia N.","family":"Michki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edwin","family":"Tamashiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noam A.","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harry","family":"Ischiropoulos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.niox.2025.12.002_bib1","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1001\/jama.1996.03540140074032","article-title":"The 1996 albert lasker medical research awards. The discovery of endothelium-derived relaxing factor and its importance in the identification of nitric oxide","volume":"276","author":"Furchgott","year":"1996","journal-title":"JAMA"},{"key":"10.1016\/j.niox.2025.12.002_bib2","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1056\/NEJMsa063904","article-title":"Shattuck lecture. Nitric oxide and cyclic GMP in cell signaling and drug development","volume":"355","author":"Murad","year":"2006","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.niox.2025.12.002_bib3","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1023\/A:1020150124721","article-title":"Nitric oxide: a unique endogenous signaling molecule in vascular biology","volume":"19","author":"Ignarro","year":"1999","journal-title":"Biosci. Rep."},{"key":"10.1016\/j.niox.2025.12.002_bib4","doi-asserted-by":"crossref","first-page":"S193","DOI":"10.1038\/sj.bjp.0706458","article-title":"The discovery of nitric oxide and its role in vascular biology","volume":"147","author":"Moncada","year":"2006","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.niox.2025.12.002_bib5","doi-asserted-by":"crossref","first-page":"4064","DOI":"10.1021\/ac9913255","article-title":"Simultaneous derivatization and quantification of the nitric oxide metabolites nitrite and nitrate in biological fluids by gas chromatography\/mass spectrometry","volume":"72","author":"Tsikas","year":"2000","journal-title":"Anal Chem."},{"issue":"13","key":"10.1016\/j.niox.2025.12.002_bib6","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1096\/fj.02-0363com","article-title":"N-, and heme-nitros(yl)ation in biological tissues and fluids: implications for the fate of NO in vivo","volume":"16","author":"Feelisch","year":"2002","journal-title":"FASEB J."},{"key":"10.1016\/j.niox.2025.12.002_bib7","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1016\/S0891-5849(02)01183-8","article-title":"Concomitant presence of N-nitroso and S-nitroso proteins in human plasma","volume":"33","author":"Rassaf","year":"2002","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.niox.2025.12.002_bib8","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0090-4295(97)00257-4","article-title":"Urinary nitrite: more than a marker of infection","volume":"50","author":"Lundberg","year":"1997","journal-title":"Urology"},{"key":"10.1016\/j.niox.2025.12.002_bib9","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1172\/JCI19019","article-title":"Nitrite in saliva increases gastric mucosal flow and mucus thickness","volume":"113","author":"Bjorne","year":"2004","journal-title":"J. Clin. Investig."},{"key":"10.1016\/j.niox.2025.12.002_bib10","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1073\/pnas.89.1.444","article-title":"S-nitrosylation of proteins with nitric oxide: synthesis and characterization of biologically active compounds","volume":"89","author":"Stamler","year":"1992","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1016\/j.niox.2025.12.002_bib11","doi-asserted-by":"crossref","first-page":"11482","DOI":"10.1073\/pnas.97.21.11482","article-title":"Role of circulating nitrite and S-nitrosohemoglobin in the regulation of the regional blood flow in humans","volume":"97","author":"Gladwin","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"issue":"1\u20132","key":"10.1016\/j.niox.2025.12.002_bib12","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jchromb.2006.07.054","article-title":"Analysis of nitrite and nitrate in biological fluids by assays based on the griess reaction: appraisal of the griess reaction in the L-arginine\/nitric oxide area of research","volume":"851","author":"Tsikas","year":"2007","journal-title":"J. Chromatogr., B: Anal. Technol. Biomed. Life Sci."},{"issue":"1\u20132","key":"10.1016\/j.niox.2025.12.002_bib13","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.jchromb.2007.02.003","article-title":"Comparison of nitrite\/nitrate concentration in human plasma and serum samples measured by the enzymatic batch griess assay, ion-pairing HPLC and ion-trap GC-MS: the importance of a correct removal of proteins in the griess assay","volume":"851","author":"Romitelli","year":"2007","journal-title":"J. Chromatogr. B"},{"issue":"1\u20132","key":"10.1016\/j.niox.2025.12.002_bib14","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jchromb.2006.07.018","article-title":"Analysis of nitrite and nitrate in biological samples using high-performance liquid chromatography","volume":"851","author":"Jobgen","year":"2007","journal-title":"J. Chromatogr. B"},{"issue":"1","key":"10.1016\/j.niox.2025.12.002_bib15","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.ab.2009.09.047","article-title":"Evidence by gas chromatography-mass spectrometry of ex vivo nitrite and nitrate formation from air nitrogen oxides in human plasma, serum, and urine samples","volume":"397","author":"Tsikas","year":"2010","journal-title":"Anal. Biochem."},{"issue":"7","key":"10.1016\/j.niox.2025.12.002_bib16","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1093\/chromsci\/bmu212","article-title":"The determination of nitrate and nitrite in human urine and blood by high-performance liquid chromatography and cloud-point extraction","volume":"53","author":"Zhao","year":"2015","journal-title":"J. Chromatogr. Sci."},{"issue":"17\u201318","key":"10.1016\/j.niox.2025.12.002_bib17","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1016\/j.jchromb.2010.05.011","article-title":"Stable-isotope dilution GC-MS approach for nitrite quantification in human whole blood, erythrocytes, and plasma using pentafluorobenzyl bromide derivatization: nitrite distribution in human blood","volume":"879","author":"Schwarz","year":"2011","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.niox.2025.12.002_bib18","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.jchromb.2016.03.034","article-title":"Simultaneous GC-ECNICI-MS measurement of nitrite, nitrate and creatinine in human urine and plasma in clinical settings","volume":"1047","author":"Hanff","year":"2017","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.niox.2025.12.002_bib19","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.freeradbiomed.2021.08.237","article-title":"Serum nitrite and nitrate: a potential biomarker for post-covid-19 complications?","volume":"175","author":"Wang","year":"2021","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.niox.2025.12.002_bib20","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1165\/rcmb.2005-0182OC","article-title":"Pulmonary and systemic nitric oxide metabolites in a baboon model of neonatal chronic lung disease","volume":"33","author":"Munson","year":"2005","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"issue":"11","key":"10.1016\/j.niox.2025.12.002_bib21","doi-asserted-by":"crossref","DOI":"10.1161\/JAHA.117.006224","article-title":"Plasma nitrate and incidence of cardiovascular disease and all-cause mortality in the community: the framingham offspring study","volume":"6","author":"Maas","year":"2017","journal-title":"J. Am. Heart Assoc."},{"issue":"Suppl 1","key":"10.1016\/j.niox.2025.12.002_bib22","first-page":"S67","article-title":"The quality control system","volume":"29","author":"Badrick","year":"2008","journal-title":"Clin. Biochem. Rev."},{"key":"10.1016\/j.niox.2025.12.002_bib23","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/clinchem\/27.3.493","article-title":"A multi-rule shewhart chart for quality control in clinical chemistry","volume":"27","author":"Westgard","year":"1981","journal-title":"Clin. Chem."},{"issue":"6","key":"10.1016\/j.niox.2025.12.002_bib24","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1208\/s12248-014-9644-1","article-title":"Repeat analysis and incurred sample reanalysis: recommendation for best practices and harmonization from the global bioanalysis consortium harmonization team","volume":"16","author":"Fluhler","year":"2014","journal-title":"AAPS J."},{"key":"10.1016\/j.niox.2025.12.002_bib25","article-title":"Guidance for industry: bioanalytical method validation. Draft guidance. US department of health and human services","year":"2018","journal-title":"Food Drug Admin."},{"issue":"3","key":"10.1016\/j.niox.2025.12.002_bib26","first-page":"1","article-title":"Better way to Do R&R studies. The evaluating the measurement process approach","volume":"39","author":"Wheeler","year":"2006","journal-title":"Qual. Prog."},{"issue":"9","key":"10.1016\/j.niox.2025.12.002_bib27","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1016\/j.theriogenology.2010.01.003","article-title":"Method agreement analysis: a review of correct methodology","volume":"73","author":"Watson","year":"2010","journal-title":"Theriogenology"},{"issue":"3","key":"10.1016\/j.niox.2025.12.002_bib28","doi-asserted-by":"crossref","first-page":"317","DOI":"10.21037\/cdt.2017.03.12","article-title":"Assessing observer variability: a users guide","volume":"7","author":"Popovi\u0107","year":"2017","journal-title":"Cardiovasc. Diagn. Ther."},{"issue":"7","key":"10.1016\/j.niox.2025.12.002_bib29","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1161\/CIRCRESAHA.116.309832","article-title":"Pharmacokinetics and pharmacodynamics of inorganic nitrate in heart failure with preserved ejection fraction","volume":"120","author":"Zamani","year":"2017","journal-title":"Circ. Res."},{"issue":"12","key":"10.1016\/j.niox.2025.12.002_bib30","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1016\/j.amjcard.2016.08.077","article-title":"Effect of heart failure with preserved ejection fraction on nitric oxide metabolites","volume":"118","author":"Zamani","year":"2016","journal-title":"Am. J. Cardiol."},{"key":"10.1016\/j.niox.2025.12.002_bib31","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.freeradbiomed.2024.11.010","article-title":"Nitrate and nitrite bioavailability in plasma and saliva: their association with blood pressure \u2013 a systematic review and meta-analysis","volume":"226","author":"Bescos","year":"2025","journal-title":"Free Radic. Biol. Med."},{"issue":"5","key":"10.1016\/j.niox.2025.12.002_bib32","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1002\/med.20151","article-title":"Nitrite as regulator of hypoxic signaling in mammalian physiology","volume":"29","author":"van Faassen","year":"2009","journal-title":"Med. Res. Rev."},{"key":"10.1016\/j.niox.2025.12.002_bib33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.niox.2015.01.001","article-title":"Circulating and excretory nitrite and nitrate: their value as measures of nitric oxide synthesis, bioavailability and activity is inherently limited","volume":"45","author":"Tsikas","year":"2015","journal-title":"Nitric Oxide"},{"key":"10.1016\/j.niox.2025.12.002_bib34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.niox.2014.10.003","article-title":"Pitfalls in measuring NO bioavailability using Nox","volume":"44","author":"Kim-Shapiro","year":"2015","journal-title":"Nitric Oxide"},{"key":"10.1016\/j.niox.2025.12.002_bib35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3945\/ajcn.2008.27131","article-title":"Food sources of nitrates and nitrites: the physiologic context for potential health benefits","volume":"90","author":"Hord","year":"2009","journal-title":"Am. J. Clin. Nutr."},{"key":"10.1016\/j.niox.2025.12.002_bib36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.niox.2022.06.007","article-title":"Reduced nitric oxide synthesis in winter: a potential contributing factor to increased cardiovascular risk","volume":"127","author":"Liddle","year":"2022","journal-title":"Nitric Oxide"},{"key":"10.1016\/j.niox.2025.12.002_bib37","doi-asserted-by":"crossref","first-page":"17593","DOI":"10.1073\/pnas.0707462104","article-title":"Higher blood flow and circulating NO products offset high-altitude hypoxia among tibetans","volume":"104","author":"Erzurum","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U.S.A"}],"container-title":["Nitric Oxide"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1089860325001053?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1089860325001053?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T19:16:55Z","timestamp":1773947815000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1089860325001053"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":37,"alternative-id":["S1089860325001053"],"URL":"https:\/\/doi.org\/10.1016\/j.niox.2025.12.002","relation":{},"ISSN":["1089-8603"],"issn-type":[{"value":"1089-8603","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Analytic performance characteristics of the chemical-reduction gas phase-chemiluminescence assay for the quantification of nitric oxide metabolites","name":"articletitle","label":"Article Title"},{"value":"Nitric Oxide","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.niox.2025.12.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}