{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T03:13:52Z","timestamp":1788146032210,"version":"build-2803163510"},"reference-count":25,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.es","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Archivos de la Sociedad Espa\u00f1ola de Oftalmolog\u00eda (English Edition)"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.oftale.2026.502582","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T15:46:57Z","timestamp":1781279217000},"page":"502582","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic applications of gene editing using CRISPR-Cas9 in the posterior segment: review of the literature"],"prefix":"10.1016","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2801-7988","authenticated-orcid":false,"given":"J.A.","family":"Leon Agudelo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Mart\u00ednez Vallejo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.oftale.2026.502582_bib0005","first-page":"321","article-title":"Gene therapy for inherited retinal diseases","volume":"130","author":"Bennett","year":"2023","journal-title":"Ophthalmology."},{"issue":"6213","key":"10.1016\/j.oftale.2026.502582_bib0010","doi-asserted-by":"crossref","DOI":"10.1126\/science.1258096","article-title":"The new frontier of genome engineering with CRISPR-Cas9","volume":"346","author":"Doudna","year":"2014","journal-title":"Science."},{"issue":"6","key":"10.1016\/j.oftale.2026.502582_bib0015","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1016\/j.cell.2014.05.010","article-title":"Development and applications of CRISPR-Cas9 for genome engineering","volume":"157","author":"Hsu","year":"2014","journal-title":"Cell."},{"issue":"1-2","key":"10.1016\/j.oftale.2026.502582_bib0020","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.cell.2016.10.044","article-title":"CRISPR-based technologies for the manipulation of eukaryotic genomes","volume":"168","author":"Komor","year":"2017","journal-title":"Cell."},{"issue":"12","key":"10.1016\/j.oftale.2026.502582_bib0025","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1038\/s41576-018-0059-1","article-title":"Base editing: precision chemistry on the genome and transcriptome of living cells","volume":"19","author":"Rees","year":"2018","journal-title":"Nat Rev Genet."},{"issue":"5","key":"10.1016\/j.oftale.2026.502582_bib0030","first-page":"301","article-title":"Adeno-associated virus gene therapy in retinal disorders","volume":"33","author":"Bennett","year":"2022","journal-title":"Hum Gene Ther."},{"issue":"8","key":"10.1016\/j.oftale.2026.502582_bib0035","first-page":"1235","article-title":"In vivo delivery of base editors to the retina using engineered AAV capsids","volume":"40","author":"Tornabene","year":"2022","journal-title":"Nat Biotechnol."},{"key":"10.1016\/j.oftale.2026.502582_bib0040","article-title":"CRISPR\/Cas9-based gene therapy for inherited retinal diseases","volume":"94","author":"J\u00fcttner","year":"2024","journal-title":"Prog Retin Eye Res."},{"key":"10.1016\/j.oftale.2026.502582_bib0045","article-title":"Correction of the CEP290 IVS26 mutation using CRISPR-Cas9","volume":"8","author":"Yin","year":"2017","journal-title":"Nat Commun."},{"issue":"2","key":"10.1016\/j.oftale.2026.502582_bib0050","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/s41591-018-0327-9","article-title":"In vivo editing of the human CEP290 gene in the retina","volume":"25","author":"Maeder","year":"2019","journal-title":"Nat Med."},{"issue":"3","key":"10.1016\/j.oftale.2026.502582_bib0055","first-page":"456","article-title":"In vivo base editing restores visual function in mouse models of retinitis pigmentosa","volume":"41","author":"Yeh","year":"2023","journal-title":"Nat Biotechnol."},{"issue":"11","key":"10.1016\/j.oftale.2026.502582_bib0060","first-page":"1310","article-title":"Investigation of Cas9-induced large deletions and rearrangements","volume":"38","author":"Zuo","year":"2020","journal-title":"Nat Biotechnol."},{"issue":"5","key":"10.1016\/j.oftale.2026.502582_bib0065","first-page":"1420","article-title":"RNA-targeting CRISPR-Cas13 therapy for neovascular eye disease","volume":"31","author":"Li","year":"2023","journal-title":"Mol Ther."},{"issue":"2","key":"10.1016\/j.oftale.2026.502582_bib0070","article-title":"Long-term VEGFA silencing using Cas13-mediated RNA editing","volume":"3","author":"Hou","year":"2024","journal-title":"Ophthalmol Sci."},{"issue":"9","key":"10.1016\/j.oftale.2026.502582_bib0075","first-page":"1558","article-title":"BRILLIANCE trial results for EDIT-101","volume":"29","year":"2023","journal-title":"Nat Med."},{"issue":"7","key":"10.1016\/j.oftale.2026.502582_bib0080","first-page":"561","article-title":"Dual AAV-mediated knock-out and replacement of RHO gene","volume":"35","author":"Gootkin","year":"2024","journal-title":"Hum Gene Ther."},{"key":"10.1016\/j.oftale.2026.502582_bib0085","series-title":"Clinical trial HG202: NCT06031727","year":"2024"},{"issue":"7587","key":"10.1016\/j.oftale.2026.502582_bib0090","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1038\/nature16526","article-title":"High-fidelity CRISPR\u2013Cas9 nucleases with no detectable genome-wide off-target effects","volume":"529","author":"Kleinstiver","year":"2016","journal-title":"Nature."},{"issue":"2","key":"10.1016\/j.oftale.2026.502582_bib0095","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1038\/s41591-018-0326-x","article-title":"Identification of pre-existing adaptive immunity to Cas9 proteins","volume":"25","author":"Charlesworth","year":"2019","journal-title":"Nat Med."},{"issue":"7546","key":"10.1016\/j.oftale.2026.502582_bib0100","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1038\/nature14299","article-title":"In vivo genome editing using Staphylococcus aureus Cas9","volume":"520","author":"Ran","year":"2015","journal-title":"Nature."},{"issue":"1","key":"10.1016\/j.oftale.2026.502582_bib0105","first-page":"81","article-title":"Prime editing in postmitotic neurons and retina","volume":"42","author":"Yeh","year":"2024","journal-title":"Nat Biotechnol."},{"issue":"6","key":"10.1016\/j.oftale.2026.502582_bib0110","first-page":"367","article-title":"Ethical challenges of somatic and germline genome editing","volume":"24","author":"Baylis","year":"2023","journal-title":"Nat Rev Genet."},{"issue":"7785","key":"10.1016\/j.oftale.2026.502582_bib0115","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/s41586-019-1711-4","article-title":"Search-and-replace genome editing without double-strand breaks","volume":"576","author":"Anzalone","year":"2019","journal-title":"Nature."},{"key":"10.1016\/j.oftale.2026.502582_bib0120","article-title":"Lipid nanoparticle delivery of CRISPR components to the eye","volume":"203","author":"Wang","year":"2024","journal-title":"Adv Drug Deliv Rev."},{"key":"10.1016\/j.oftale.2026.502582_bib0125","article-title":"Integration of CRISPR editing and stem cell therapy in retinal regeneration","volume":"95","author":"Li","year":"2025","journal-title":"Prog Retin Eye Res."}],"container-title":["Archivos de la Sociedad Espa\u00f1ola de Oftalmolog\u00eda (English Edition)"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2173579426001441?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2173579426001441?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T02:20:37Z","timestamp":1788142837000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2173579426001441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":25,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["S2173579426001441"],"URL":"https:\/\/doi.org\/10.1016\/j.oftale.2026.502582","relation":{},"ISSN":["2173-5794"],"issn-type":[{"value":"2173-5794","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Therapeutic applications of gene editing using CRISPR-Cas9 in the posterior segment: review of the literature","name":"articletitle","label":"Article Title"},{"value":"Archivos de la Sociedad Espa\u00f1ola de Oftalmolog\u00eda (English Edition)","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.oftale.2026.502582","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.oftal.2026.502582","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Sociedad Espa\u00f1ola de Oftalmolog\u00eda. Published by Elsevier Espa\u00f1a, S.L.U. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"502582"}}