{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T16:06:56Z","timestamp":1789056416854,"version":"build-2803163510"},"reference-count":28,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004337","name":"F Hoffmann-La Roche AG","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004337","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy Advances"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.omta.2026.201833","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T06:47:11Z","timestamp":1786085231000},"page":"201833","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["RNA-TITER\u2014An ADAR-based transcript detection assay for accelerated functional titer assessment of adeno-associated viruses"],"prefix":"10.1016","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7799-2413","authenticated-orcid":false,"given":"Tanja","family":"Rothgangl","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Carullo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Ohland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2001-9406","authenticated-orcid":false,"given":"Sandra","family":"Kemptner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Phichith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluigi","family":"Lichinchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.omta.2026.201833_bib1","doi-asserted-by":"crossref","DOI":"10.3390\/biom14070854","article-title":"Recent Advances in Gene Therapy for Hemophilia: Projecting the Perspectives","volume":"14","author":"Chernyi","year":"2024","journal-title":"Biomolecules"},{"key":"10.1016\/j.omta.2026.201833_bib2","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.1016\/j.ymthe.2025.03.026","article-title":"Recent advances in therapeutic gene-editing technologies","volume":"33","author":"Liu","year":"2025","journal-title":"Mol. Ther."},{"key":"10.1016\/j.omta.2026.201833_bib3","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1038\/nprot.2006.236","article-title":"Quantification of mRNA using real-time RT-PCR","volume":"1","author":"Nolan","year":"2006","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.omta.2026.201833_bib4","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/s41587-022-01617-3","article-title":"Author Correction: Modular, programmable RNA sensing using ADAR editing in living cells","volume":"41","author":"Kaseniit","year":"2023","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.omta.2026.201833_bib5","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/s41587-022-01534-5","article-title":"Programmable eukaryotic protein synthesis with RNA sensors by harnessing ADAR","volume":"41","author":"Jiang","year":"2023","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.omta.2026.201833_bib6","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/bs.mie.2024.11.027","article-title":"CellREADR: An ADAR-based RNA sensor-actuator device","volume":"710","author":"Yang","year":"2025","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.omta.2026.201833_bib7","doi-asserted-by":"crossref","first-page":"2860","DOI":"10.1002\/1873-3468.13093","article-title":"All I\u2019s on the RADAR: role of ADAR in gene regulation","volume":"592","author":"Shevchenko","year":"2018","journal-title":"FEBS Lett."},{"key":"10.1016\/j.omta.2026.201833_bib8","doi-asserted-by":"crossref","first-page":"3165","DOI":"10.1021\/acs.accounts.3c00433","article-title":"Adenosine Deaminase Acting on RNA (ADAR) Enzymes: A Journey from Weird to Wondrous","volume":"56","author":"Keegan","year":"2023","journal-title":"Acc. Chem. Res."},{"key":"10.1016\/j.omta.2026.201833_bib9","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21124197","article-title":"Recombinant Adeno-Associated Viral Vectors (rAAV)-Vector Elements in Ocular Gene Therapy Clinical Trials and Transgene Expression and Bioactivity Assays","volume":"21","author":"Buck","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.omta.2026.201833_bib10","doi-asserted-by":"crossref","DOI":"10.1101\/pdb.prot5421","article-title":"Luciferase assay","volume":"2010","author":"Smale","year":"2010","journal-title":"Cold Spring Harb. Protoc."},{"key":"10.1016\/j.omta.2026.201833_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2025.108720","article-title":"Gene expression and protein abundance: Just how associated are these molecular traits?","volume":"86","author":"Samih","year":"2026","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.omta.2026.201833_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.xphs.2026.104257","article-title":"Comprehensive stress study on recombinant adeno-associated virus vectors: Evaluating the capabilities of established analytical techniques","volume":"115","author":"Heckel","year":"2026","journal-title":"J. Pharm. Sci."},{"key":"10.1016\/j.omta.2026.201833_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtm.2025.101416","article-title":"Development of cell lines with increased susceptibility to diverse adeno-associated viral vectors to enable potency assays","volume":"33","author":"Zengel","year":"2025","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"10.1016\/j.omta.2026.201833_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtm.2024.101401","article-title":"A versatile cell line for establishing potency of cell type-specific AAV transgenes","volume":"33","author":"Martinez","year":"2025","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"10.1016\/j.omta.2026.201833_bib15","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.omtm.2019.03.004","article-title":"CRISPR Activation Enhances Potency of AAV Vectors Driven by Tissue-Specific Promoters","volume":"13","author":"McDougald","year":"2019","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"10.1016\/j.omta.2026.201833_bib16","doi-asserted-by":"crossref","DOI":"10.3791\/68646","article-title":"Scaling Down for Big Impact: Streamlined High-throughput Recombinant Adeno-associated Virus Production","author":"Ohland","year":"2025","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.omta.2026.201833_bib17","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.3389\/fmicb.2019.01570","article-title":"Accurate Quantification and Characterization of Adeno-Associated Viral Vectors","volume":"10","author":"Dobnik","year":"2019","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.omta.2026.201833_bib18","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1089\/hgtb.2011.034","article-title":"Universal Real-Time PCR for the Detection and Quantification of Adeno-Associated Virus Serotype 2-Derived Inverted Terminal Repeat Sequences","volume":"23","author":"Aurnhammer","year":"2012","journal-title":"Hum. Gene Ther. Methods"},{"key":"10.1016\/j.omta.2026.201833_bib19","unstructured":"ICH Q2(R2) Guideline on validation of analytical procedures https:\/\/www.ema.europa.eu\/en\/documents\/scientific-guideline\/ich-q2r2-guideline-validation-analytical-procedures-step-5-revision-2_en.pdf."},{"key":"10.1016\/j.omta.2026.201833_bib20","doi-asserted-by":"crossref","first-page":"e24","DOI":"10.1093\/nar\/gnh020","article-title":"Determination of detection and quantification limits for SNP allele frequency estimation in DNA pools using real time PCR","volume":"32","author":"Schwarz","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.omta.2026.201833_bib21","unstructured":"ICH guideline M10 on bioanalytical method validation and study sample analysis https:\/\/www.ema.europa.eu\/en\/documents\/scientific-guideline\/ich-guideline-m10-bioanalytical-method-validation-step-5_en.pdf."},{"key":"10.1016\/j.omta.2026.201833_bib22","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1038\/s41587-019-0032-3","article-title":"CRISPResso2 provides accurate and rapid genome editing sequence analysis","volume":"37","author":"Clement","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.omta.2026.201833_bib23","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"10.1016\/j.omta.2026.201833_bib24","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.omta.2026.201833_bib25","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: an efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Liao","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.omta.2026.201833_bib26","doi-asserted-by":"crossref","DOI":"10.1093\/gigascience\/giab008","article-title":"Twelve years of SAMtools and BCFtools","volume":"10","author":"Danecek","year":"2021","journal-title":"GigaScience"},{"key":"10.1016\/j.omta.2026.201833_bib27","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1093\/bioinformatics\/bts356","article-title":"RSeQC: quality control of RNA-seq experiments","volume":"28","author":"Wang","year":"2012","journal-title":"Bioinformatics"},{"key":"10.1016\/j.omta.2026.201833_bib28","doi-asserted-by":"crossref","first-page":"3047","DOI":"10.1093\/bioinformatics\/btw354","article-title":"MultiQC: summarize analysis results for multiple tools and samples in a single report","volume":"32","author":"Ewels","year":"2016","journal-title":"Bioinformatics"}],"container-title":["Molecular Therapy Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S3117387X26001680?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S3117387X26001680?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T15:14:51Z","timestamp":1789053291000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S3117387X26001680"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["S3117387X26001680"],"URL":"https:\/\/doi.org\/10.1016\/j.omta.2026.201833","relation":{},"ISSN":["3117-387X"],"issn-type":[{"value":"3117-387X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"RNA-TITER\u2014An ADAR-based transcript detection assay for accelerated functional titer assessment of adeno-associated viruses","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy Advances","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.omta.2026.201833","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc. on behalf of The American Society of Gene and Cell Therapy.","name":"copyright","label":"Copyright"}],"article-number":"201833"}}