{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T05:42:48Z","timestamp":1776750168557,"version":"3.51.2"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,6,26]],"date-time":"2020-06-26T00:00:00Z","timestamp":1593129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy - Methods & Clinical Development"],"published-print":{"date-parts":[[2020,9]]},"DOI":"10.1016\/j.omtm.2020.06.023","type":"journal-article","created":{"date-parts":[[2020,6,30]],"date-time":"2020-06-30T19:56:29Z","timestamp":1593546989000},"page":"511-519","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Direct Functional Protein Delivery with a Peptide into Neonatal and Adult Mammalian Inner Ear In\u00a0Vivo"],"prefix":"10.1016","volume":"18","author":[{"given":"Kun","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoting","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingqian","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Tao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongbo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica M.","family":"Rosenholm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng-Yi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yilai","family":"Shu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.omtm.2020.06.023_bib1","doi-asserted-by":"crossref","first-page":"2503","DOI":"10.1016\/S0140-6736(17)31073-5","article-title":"Global hearing health care: new findings and perspectives","volume":"390","author":"Wilson","year":"2017","journal-title":"Lancet"},{"key":"10.1016\/j.omtm.2020.06.023_bib2","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1056\/NEJMra1616601","article-title":"Hearing Loss in Adults","volume":"377","author":"Cunningham","year":"2017","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.omtm.2020.06.023_bib3","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1038\/nrd4533","article-title":"New treatment options for hearing loss","volume":"14","author":"M\u00fcller","year":"2015","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.omtm.2020.06.023_bib4","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1038\/nm.3343","article-title":"Toward better representations of sound with cochlear implants","volume":"19","author":"Wilson","year":"2013","journal-title":"Nat. Med."},{"key":"10.1016\/j.omtm.2020.06.023_bib5","doi-asserted-by":"crossref","first-page":"8","DOI":"10.3389\/fncel.2019.00008","article-title":"Transduction of Adeno-Associated Virus Vectors Targeting Hair Cells and Supporting Cells in the Neonatal Mouse Cochlea","volume":"13","author":"Gu","year":"2019","journal-title":"Front. Cell. Neurosci."},{"key":"10.1016\/j.omtm.2020.06.023_bib6","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1038\/s41467-018-08264-w","article-title":"Improved TMC1 gene therapy restores hearing and balance in mice with genetic inner ear disorders","volume":"10","author":"Nist-Lund","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omtm.2020.06.023_bib7","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/nbt.3081","article-title":"Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in\u00a0vitro and in\u00a0vivo","volume":"33","author":"Zuris","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.omtm.2020.06.023_bib8","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1038\/s41467-018-03779-8","article-title":"The fluorination effect of fluoroamphiphiles in cytosolic protein delivery","volume":"9","author":"Zhang","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omtm.2020.06.023_bib9","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/nchem.2779","article-title":"Cytosolic antibody delivery by lipid-sensitive endosomolytic peptide","volume":"9","author":"Akishiba","year":"2017","journal-title":"Nat. Chem."},{"key":"10.1016\/j.omtm.2020.06.023_bib10","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/nmat4269","article-title":"In\u00a0vivo delivery of transcription factors with multifunctional oligonucleotides","volume":"14","author":"Lee","year":"2015","journal-title":"Nat. Mater."},{"key":"10.1016\/j.omtm.2020.06.023_bib11","doi-asserted-by":"crossref","first-page":"10583","DOI":"10.1002\/anie.201505437","article-title":"Protein Delivery System Containing a Nickel-Immobilized Polymer for Multimerization of Affinity-Purified His-Tagged Proteins Enhances Cytosolic Transfer","volume":"54","author":"Postupalenko","year":"2015","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.omtm.2020.06.023_bib12","doi-asserted-by":"crossref","first-page":"2756","DOI":"10.1039\/C9BM01872G","article-title":"Core-shell particles for drug-delivery, bioimaging, sensing, and tissue engineering","volume":"8","author":"Jenjob","year":"2020","journal-title":"Biomater. Sci."},{"key":"10.1016\/j.omtm.2020.06.023_bib13","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1615\/CritRevTherDrugCarrierSyst.2013006955","article-title":"Peptide and protein delivery using new drug delivery systems","volume":"30","author":"Jain","year":"2013","journal-title":"Crit. Rev. Ther. Drug Carrier Syst."},{"key":"10.1016\/j.omtm.2020.06.023_bib14","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.biomaterials.2017.04.036","article-title":"Protein based therapeutic delivery agents: Contemporary developments and challenges","volume":"134","author":"Yin","year":"2017","journal-title":"Biomaterials"},{"key":"10.1016\/j.omtm.2020.06.023_bib15","doi-asserted-by":"crossref","first-page":"13194","DOI":"10.1038\/s41598-017-13469-y","article-title":"A quantitative comparison of cytosolic delivery via different protein uptake systems","volume":"7","author":"Verdurmen","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.omtm.2020.06.023_bib16","doi-asserted-by":"crossref","first-page":"14084","DOI":"10.1021\/jacs.5b05694","article-title":"Discovery and characterization of a peptide that enhances endosomal escape of delivered proteins in\u00a0vitro and in\u00a0vivo","volume":"137","author":"Li","year":"2015","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.omtm.2020.06.023_bib17","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/415389a","article-title":"Antimicrobial peptides of multicellular organisms","volume":"415","author":"Zasloff","year":"2002","journal-title":"Nature"},{"key":"10.1016\/j.omtm.2020.06.023_bib18","doi-asserted-by":"crossref","first-page":"49","DOI":"10.3390\/ph2020049","article-title":"Fusion of a Short HA2-Derived Peptide Sequence to Cell-Penetrating Peptides Improves Cytosolic Uptake, but Enhances Cytotoxic Activity","volume":"2","author":"Neundorf","year":"2009","journal-title":"Pharmaceuticals (Basel)"},{"key":"10.1016\/j.omtm.2020.06.023_bib19","doi-asserted-by":"crossref","first-page":"241","DOI":"10.2174\/1386207053764576","article-title":"Molecular mechanisms of membrane perturbation by antimicrobial peptides and the use of biophysical studies in the design of novel peptide antibiotics","volume":"8","author":"Lohner","year":"2005","journal-title":"Comb. Chem. High Throughput Screen."},{"key":"10.1016\/j.omtm.2020.06.023_bib20","first-page":"324","article-title":"Form and function of the mammalian inner ear","volume":"228","author":"Ekdale","year":"2016","journal-title":"J.\u00a0Anat."},{"key":"10.1016\/j.omtm.2020.06.023_bib21","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1111\/j.1749-6632.2009.04484.x","article-title":"Development and regeneration of the inner ear","volume":"1170","author":"Kwan","year":"2009","journal-title":"Ann. N Y Acad. Sci."},{"key":"10.1016\/j.omtm.2020.06.023_bib22","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.addr.2016.01.004","article-title":"Advances in nano-based inner ear delivery systems for the treatment of sensorineural hearing loss","volume":"108","author":"Li","year":"2017","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.omtm.2020.06.023_bib23","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1038\/nm996","article-title":"Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis","volume":"10","author":"Wadia","year":"2004","journal-title":"Nat. Med."},{"key":"10.1016\/j.omtm.2020.06.023_bib24","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.tips.2016.10.005","article-title":"Emerging Strategies for Developing Next-Generation Protein Therapeutics for Cancer Treatment","volume":"37","author":"Kintzing","year":"2016","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1016\/j.omtm.2020.06.023_bib25","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1038\/sj.bjp.0707678","article-title":"Comparative study on transduction and toxicity of protein transduction domains","volume":"153","author":"Sugita","year":"2008","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.omtm.2020.06.023_bib26","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1097\/WNR.0b013e32834da8f8","article-title":"Protein transduction into the mouse otocyst using arginine-rich cell-penetrating peptides","volume":"22","author":"Miwa","year":"2011","journal-title":"Neuroreport"},{"key":"10.1016\/j.omtm.2020.06.023_bib27","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.biomaterials.2015.09.025","article-title":"Intratympanic delivery of oligoarginine-conjugated nanoparticles as a gene (or drug) carrier to the inner ear","volume":"73","author":"Yoon","year":"2015","journal-title":"Biomaterials"},{"key":"10.1016\/j.omtm.2020.06.023_bib28","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1038\/nature04849","article-title":"Mammalian cochlear supporting cells can divide and trans-differentiate into hair cells","volume":"441","author":"White","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.omtm.2020.06.023_bib29","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1002\/lary.25995","article-title":"Peripheral vestibular pathology in Mondini dysplasia","volume":"127","author":"Kaya","year":"2017","journal-title":"Laryngoscope"},{"key":"10.1016\/j.omtm.2020.06.023_bib30","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1126\/scitranslmed.aao0935","article-title":"Delivery of therapeutics to the inner ear: The challenge of the blood-labyrinth barrier","volume":"11","author":"Nyberg","year":"2019","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.omtm.2020.06.023_bib31","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1517\/17425247.2015.974548","article-title":"The role of intracochlear drug delivery devices in the management of inner ear disease","volume":"12","author":"Ayoob","year":"2015","journal-title":"Expert Opin. Drug Deliv."},{"key":"10.1016\/j.omtm.2020.06.023_bib32","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1007\/s13346-017-0384-5","article-title":"Innovative pharmaceutical approaches for the management of inner ear disorders","volume":"8","author":"Musazzi","year":"2018","journal-title":"Drug Deliv. Transl. Res."},{"key":"10.1016\/j.omtm.2020.06.023_bib33","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.ejps.2018.05.020","article-title":"Inner ear drug delivery: Recent advances, challenges, and perspective","volume":"126","author":"Hao","year":"2019","journal-title":"Eur. J. Pharm. Sci."},{"key":"10.1016\/j.omtm.2020.06.023_bib34","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.heares.2018.06.017","article-title":"Anatomical basis of drug delivery to the inner ear","volume":"368","author":"Glueckert","year":"2018","journal-title":"Hear. Res."},{"key":"10.1016\/j.omtm.2020.06.023_bib35","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1089\/hum.2016.053","article-title":"Identification of Adeno-Associated Viral Vectors That Target Neonatal and Adult Mammalian Inner Ear Cell Subtypes","volume":"27","author":"Shu","year":"2016","journal-title":"Hum. Gene Ther."}],"container-title":["Molecular Therapy - Methods & Clinical Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2329050120301479?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2329050120301479?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T09:32:14Z","timestamp":1762421534000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2329050120301479"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9]]},"references-count":35,"alternative-id":["S2329050120301479"],"URL":"https:\/\/doi.org\/10.1016\/j.omtm.2020.06.023","relation":{},"ISSN":["2329-0501"],"issn-type":[{"value":"2329-0501","type":"print"}],"subject":[],"published":{"date-parts":[[2020,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Direct Functional Protein Delivery with a Peptide into Neonatal and Adult Mammalian Inner Ear In\u00a0Vivo","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy - Methods & Clinical Development","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.omtm.2020.06.023","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Author(s).","name":"copyright","label":"Copyright"}]}}