{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:05:17Z","timestamp":1788455117309,"version":"build-2803163510"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T00:00:00Z","timestamp":1679011200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy - Methods &amp; Clinical Development"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1016\/j.omtm.2023.03.009","type":"journal-article","created":{"date-parts":[[2023,3,21]],"date-time":"2023-03-21T11:06:28Z","timestamp":1679396788000},"page":"202-212","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"special_numbering":"C","title":["Channelrhodopsin fluorescent tag replacement for clinical translation of optogenetic hearing restoration"],"prefix":"10.1016","volume":"29","author":[{"given":"Maria","family":"Zerche","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Wrobel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"Kusch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Moser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9571-7033","authenticated-orcid":false,"given":"Thomas","family":"Mager","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.omtm.2023.03.009_bib1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.neuron.2006.02.026","article-title":"Ectopic expression of a microbial-type rhodopsin restores visual responses in mice with photoreceptor degeneration","volume":"50","author":"Bi","year":"2006","journal-title":"Neuron"},{"key":"10.1016\/j.omtm.2023.03.009_bib2","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1038\/s41591-021-01351-4","article-title":"Partial recovery of visual function in a blind patient after optogenetic therapy","volume":"27","author":"Sahel","year":"2021","journal-title":"Nat. Med."},{"key":"10.1016\/j.omtm.2023.03.009_bib3","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aao0540","article-title":"Optogenetic stimulation of cochlear neurons activates the auditory pathway and restores auditory-driven behavior in deaf adult gerbils","volume":"10","author":"Wrobel","year":"2018","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.omtm.2023.03.009_bib4","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1172\/JCI69050","article-title":"Optogenetic stimulation of the auditory pathway","volume":"124","author":"Hernandez","year":"2014","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.omtm.2023.03.009_bib5","doi-asserted-by":"crossref","first-page":"2395","DOI":"10.1126\/science.1072068","article-title":"Channelrhodopsin-1: a light-gated proton channel in green algae","volume":"296","author":"Nagel","year":"2002","journal-title":"Science"},{"key":"10.1016\/j.omtm.2023.03.009_bib6","doi-asserted-by":"crossref","first-page":"13940","DOI":"10.1073\/pnas.1936192100","article-title":"Channelrhodopsin-2, a directly light-gated cation-selective membrane channel","volume":"100","author":"Nagel","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.omtm.2023.03.009_bib7","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0378-5955(98)00061-6","article-title":"Spatial resolution of cochlear implants: the electrical field and excitation of auditory afferents","volume":"121","author":"Kral","year":"1998","journal-title":"Hear. Res."},{"key":"10.1016\/j.omtm.2023.03.009_bib8","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1121\/1.1381538","article-title":"Speech recognition in noise as a function of the number of spectral channels: comparison of acoustic hearing and cochlear implants","volume":"110","author":"Friesen","year":"2001","journal-title":"J.\u00a0Acoust. Soc. Am."},{"key":"10.1016\/j.omtm.2023.03.009_bib9","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1038\/s41467-018-04146-3","article-title":"High frequency neural spiking and auditory signaling by ultrafast red-shifted optogenetics","volume":"9","author":"Mager","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omtm.2023.03.009_bib10","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.abb8086","article-title":"Multichannel optogenetic stimulation of the auditory pathway using microfabricated LED cochlear implants in rodents","volume":"12","author":"Keppeler","year":"2020","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.omtm.2023.03.009_bib11","doi-asserted-by":"crossref","first-page":"1962","DOI":"10.1038\/s41467-019-09980-7","article-title":"Near physiological spectral selectivity of cochlear optogenetics","volume":"10","author":"Dieter","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omtm.2023.03.009_bib12","doi-asserted-by":"crossref","DOI":"10.15252\/emmm.202012387","article-title":"muLED-based optical cochlear implants for spectrally selective activation of the auditory nerve","volume":"12","author":"Dieter","year":"2020","journal-title":"EMBO Mol. Med."},{"key":"10.1016\/j.omtm.2023.03.009_bib13","doi-asserted-by":"crossref","first-page":"3621","DOI":"10.1016\/j.csbj.2022.06.061","article-title":"Model-based prediction of optogenetic sound encoding in the human cochlea by future optical cochlear implants","volume":"20","author":"Khurana","year":"2022","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"10.1016\/j.omtm.2023.03.009_bib14","doi-asserted-by":"crossref","DOI":"10.15252\/emmm.202013391","article-title":"Utility of red-light ultrafast optogenetic stimulation of the auditory pathway","volume":"13","author":"Bali","year":"2021","journal-title":"EMBO Mol. Med."},{"key":"10.1016\/j.omtm.2023.03.009_bib15","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1038\/nmeth.2836","article-title":"Independent optical excitation of distinct neural populations","volume":"11","author":"Klapoetke","year":"2014","journal-title":"Nat. Methods"},{"key":"10.1016\/j.omtm.2023.03.009_bib16","doi-asserted-by":"crossref","DOI":"10.15252\/embj.201899649","article-title":"Ultrafast optogenetic stimulation of the auditory pathway by targeting-optimized Chronos","volume":"37","author":"Keppeler","year":"2018","journal-title":"EMBO J."},{"key":"10.1016\/j.omtm.2023.03.009_bib17","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/s42003-020-01594-w","article-title":"Optogenetic therapy: high spatiotemporal resolution and pattern discrimination compatible with vision restoration in non-human primates","volume":"4","author":"Gauvain","year":"2021","journal-title":"Commun. Biol."},{"key":"10.1016\/j.omtm.2023.03.009_bib18","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.cell.2010.02.037","article-title":"Molecular and cellular approaches for diversifying and extending optogenetics","volume":"141","author":"Gradinaru","year":"2010","journal-title":"Cell"},{"key":"10.1016\/j.omtm.2023.03.009_bib19","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.1603187","article-title":"Inward H+ pump xenorhodopsin: Mechanism and alternative optogenetic approach","volume":"3","author":"Shevchenko","year":"2017","journal-title":"Sci. Adv."},{"key":"10.1016\/j.omtm.2023.03.009_bib20","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/j.bbamem.2006.09.008","article-title":"Regulation of G protein-coupled receptor export trafficking","volume":"1768","author":"Dong","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.omtm.2023.03.009_bib21","doi-asserted-by":"crossref","first-page":"30741","DOI":"10.1074\/jbc.M313881200","article-title":"A conserved motif for the transport of G protein-coupled receptors from the endoplasmic reticulum to the cell surface","volume":"279","author":"Duvernay","year":"2004","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.omtm.2023.03.009_bib22","doi-asserted-by":"crossref","DOI":"10.26508\/lsa.202101338","article-title":"Analyzing efficacy, stability, and safety of AAV-mediated optogenetic hearing restoration in mice","volume":"5","author":"Bali","year":"2022","journal-title":"Life Sci. Alliance"},{"key":"10.1016\/j.omtm.2023.03.009_bib23","doi-asserted-by":"crossref","first-page":"4910","DOI":"10.1016\/j.cub.2020.09.056","article-title":"Lateral gene transfer of anion-conducting channelrhodopsins between green algae and giant viruses","volume":"30","author":"Rozenberg","year":"2020","journal-title":"Curr. Biol."},{"key":"10.1016\/j.omtm.2023.03.009_bib24","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1121\/1.381736","article-title":"Auditory-nerve response from cats raised in a low-noise chamber","volume":"63","author":"Liberman","year":"1978","journal-title":"J.\u00a0Acoust. Soc. Am."},{"key":"10.1016\/j.omtm.2023.03.009_bib25","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1109\/86.372900","article-title":"Electronic design of a cochlear implant for multichannel high-rate pulsatile stimulation strategies","volume":"3","author":"Zierhofer","year":"1995","journal-title":"IEEE Trans. Rehabil. Eng."},{"key":"10.1016\/j.omtm.2023.03.009_bib26","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1016\/j.ymthe.2018.05.023","article-title":"Ancestral adeno-associated virus vector delivery of opsins to spiral ganglion neurons: implications for optogenetic cochlear implants","volume":"26","author":"Duarte","year":"2018","journal-title":"Mol. Ther."},{"key":"10.1016\/j.omtm.2023.03.009_bib27","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/nn.3078","article-title":"A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing","volume":"15","author":"Madisen","year":"2012","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.omtm.2023.03.009_bib28","doi-asserted-by":"crossref","first-page":"E2624","DOI":"10.1073\/pnas.1700269114","article-title":"Structure-guided SCHEMA recombination generates diverse chimeric channelrhodopsins","volume":"114","author":"Bedbrook","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.omtm.2023.03.009_bib29","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.ymthe.2005.10.008","article-title":"Efficient neuronal gene transfer with AAV8 leads to neurotoxic levels of tau or green fluorescent proteins","volume":"13","author":"Klein","year":"2006","journal-title":"Mol. Ther."},{"key":"10.1016\/j.omtm.2023.03.009_bib30","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1006\/bbrc.1999.0954","article-title":"Is green fluorescent protein toxic to the living cells?","volume":"260","author":"Liu","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.omtm.2023.03.009_bib31","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-32075-0","article-title":"Optogenetic peripheral nerve immunogenicity","volume":"8","author":"Maimon","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.omtm.2023.03.009_bib32","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1038\/nn.3502","article-title":"ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation","volume":"16","author":"Lin","year":"2013","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.omtm.2023.03.009_bib33","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/978-1-0716-0830-2_16","article-title":"Application of targeting-optimized chronos for stimulation of the auditory pathway","volume":"2191","author":"Huet","year":"2021","journal-title":"Methods Mol. Biol."}],"container-title":["Molecular Therapy - Methods &amp; Clinical Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2329050123000451?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2329050123000451?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T13:37:55Z","timestamp":1715953075000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2329050123000451"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":33,"alternative-id":["S2329050123000451"],"URL":"https:\/\/doi.org\/10.1016\/j.omtm.2023.03.009","relation":{},"ISSN":["2329-0501"],"issn-type":[{"value":"2329-0501","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Channelrhodopsin fluorescent tag replacement for clinical translation of optogenetic hearing restoration","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy - Methods & Clinical Development","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.omtm.2023.03.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors.","name":"copyright","label":"Copyright"}]}}