{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:34:32Z","timestamp":1786995272541,"version":"build-2736575974"},"reference-count":46,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T00:00:00Z","timestamp":1705536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007698","name":"University of Florida","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007698","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy - Methods &amp; Clinical Development"],"published-print":{"date-parts":[[2024,3]]},"DOI":"10.1016\/j.omtm.2024.101192","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T11:55:06Z","timestamp":1705924506000},"page":"101192","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Expanded specific T\u00a0cells to hypomutated regions of the SARS-CoV-2 using mRNA electroporated antigen-presenting cells"],"prefix":"10.1016","volume":"32","author":[{"given":"Elizabeth","family":"Ogando-Rivas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Castillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changlin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vrunda","family":"Trivedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingpeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Pohl-Guimar\u00e3es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnav","family":"Barpujari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate M.","family":"Candelario","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hector","family":"Mendez-Gomez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elias J.","family":"Sayour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duane A.","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.omtm.2024.101192_bib1","series-title":"Center for Systems Science and Engineering (CSSE) at the","year":"2020"},{"key":"10.1016\/j.omtm.2024.101192_bib2","doi-asserted-by":"crossref","DOI":"10.1001\/jamanetworkopen.2021.11417","article-title":"Assessment of the Frequency and Variety of Persistent Symptoms Among Patients With COVID-19: A Systematic Review","volume":"4","author":"Nasserie","year":"2021","journal-title":"JAMA Netw. Open"},{"key":"10.1016\/j.omtm.2024.101192_bib3","doi-asserted-by":"crossref","DOI":"10.1001\/jamanetworkopen.2022.38804","article-title":"Prevalence and Correlates of Long COVID Symptoms Among US Adults","volume":"5","author":"Perlis","year":"2022","journal-title":"JAMA Netw. Open"},{"key":"10.1016\/j.omtm.2024.101192_bib4","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.dsx.2020.04.020","article-title":"Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): An overview of viral structure and host response","volume":"14","author":"Astuti","year":"2020","journal-title":"Diabetes Metab. Syndr."},{"key":"10.1016\/j.omtm.2024.101192_bib6","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1038\/s41586-021-04342-0","article-title":"Enhanced fitness of SARS-CoV-2 variant of concern Alpha but not Beta","volume":"602","author":"Ulrich","year":"2022","journal-title":"Nature"},{"key":"10.1016\/j.omtm.2024.101192_bib7","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/s43856-022-00108-5","article-title":"Impact of SARS-CoV-2 Gamma lineage introduction and COVID-19 vaccination on the epidemiological landscape of a Brazilian city","volume":"2","author":"Banho","year":"2022","journal-title":"Commun. Med."},{"key":"10.1016\/j.omtm.2024.101192_bib8","article-title":"Impact of SARS-CoV-2 variants on the total CD4","volume":"2","author":"Tarke","year":"2021","journal-title":"Cell Rep. Med."},{"key":"10.1016\/j.omtm.2024.101192_bib10","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/s41392-022-00997-x","article-title":"SARS-CoV-2 Omicron variant: recent progress and future perspectives","volume":"7","author":"Fan","year":"2022","journal-title":"Signal Transduct. Target. Ther."},{"key":"10.1016\/j.omtm.2024.101192_bib11","doi-asserted-by":"crossref","first-page":"12522","DOI":"10.1021\/acsnano.0c07197","article-title":"COVID-19 Vaccine Frontrunners and Their Nanotechnology Design","volume":"14","author":"Chung","year":"2020","journal-title":"ACS Nano"},{"key":"10.1016\/j.omtm.2024.101192_bib12","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1056\/NEJMoa2101544","article-title":"Safety and Efficacy of Single-Dose Ad26.COV2.S Vaccine against Covid-19","volume":"384","author":"Sadoff","year":"2021","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.omtm.2024.101192_bib13","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/S0140-6736(20)31866-3","article-title":"Safety and immunogenicity of an rAd26 and rAd5 vector-based heterologous prime-boost COVID-19 vaccine in two formulations: two open, non-randomised phase 1\/2 studies from Russia","volume":"396","author":"Logunov","year":"2020","journal-title":"Lancet"},{"key":"10.1016\/j.omtm.2024.101192_bib14","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.1056\/NEJMoa2034577","article-title":"Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine","volume":"383","author":"Polack","year":"2020","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.omtm.2024.101192_bib15","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1056\/NEJMoa2035389","article-title":"Efficacy and Safety of the mRNA-1273 SARS-CoV-2 Vaccine","volume":"384","author":"Baden","year":"2021","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.omtm.2024.101192_bib16","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1038\/s41586-021-03681-2","article-title":"Immunogenicity of Ad26.COV2.S vaccine against SARS-CoV-2 variants in humans","volume":"596","author":"Alter","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.omtm.2024.101192_bib17","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/s41591-020-01179-4","article-title":"Phase 1\/2 trial of SARS-CoV-2 vaccine ChAdOx1 nCoV-19 with a booster dose induces multifunctional antibody responses","volume":"27","author":"Barrett","year":"2021","journal-title":"Nat. Med."},{"key":"10.1016\/j.omtm.2024.101192_bib18","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1182\/blood.2021012769","article-title":"Response to SARS-CoV-2 vaccination in patients after hematopoietic cell transplantation and CAR T-cell therapy","volume":"138","author":"Dhakal","year":"2021","journal-title":"Blood"},{"key":"10.1016\/j.omtm.2024.101192_bib19","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.ccell.2022.02.010","article-title":"Efficacy of a third SARS-CoV-2 mRNA vaccine dose among hematopoietic cell transplantation, CAR T\u00a0cell, and BiTE recipients","volume":"40","author":"Abid","year":"2022","journal-title":"Cancer Cell"},{"key":"10.1016\/j.omtm.2024.101192_bib20","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1038\/s41408-022-00684-8","article-title":"Effectiveness, immunogenicity, and safety of COVID-19 vaccines for individuals with hematological malignancies: a systematic review","volume":"12","author":"Piechotta","year":"2022","journal-title":"Blood Cancer J."},{"key":"10.1016\/j.omtm.2024.101192_bib21","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1007\/s10875-022-01223-7","article-title":"COVID-19 Vaccine Uptake and Efficacy in a National Immunodeficiency Cohort","volume":"42","author":"Ponsford","year":"2022","journal-title":"J.\u00a0Clin. Immunol."},{"key":"10.1016\/j.omtm.2024.101192_bib22","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.984376","article-title":"Impact of vaccination on hospitalization and mortality from COVID-19 in patients with primary and secondary immunodeficiency: The United Kingdom experience","volume":"13","author":"Shields","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.omtm.2024.101192_bib23","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s12943-021-01335-5","article-title":"mRNA vaccine for cancer immunotherapy","volume":"20","author":"Miao","year":"2021","journal-title":"Mol. Cancer"},{"key":"10.1016\/j.omtm.2024.101192_bib24","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1016\/j.bbmt.2019.02.024","article-title":"Excellent Outcomes of Allogeneic Hematopoietic Stem Cell Transplantation in Patients with Paroxysmal Nocturnal Hemoglobinuria: A Single-Center Study","volume":"25","author":"Liu","year":"2019","journal-title":"Biol. Blood Marrow Transplant."},{"key":"10.1016\/j.omtm.2024.101192_bib25","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1007\/s12017-021-08669-9","article-title":"Contemporary RNA Therapeutics for Glioblastoma","volume":"24","author":"Melnick","year":"2022","journal-title":"NeuroMolecular Med."},{"key":"10.1016\/j.omtm.2024.101192_bib26","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1097\/01.cji.0000211325.30525.84","article-title":"Contact-activated monocytes: efficient antigen presenting cells for the stimulation of antigen-specific T\u00a0cells","volume":"30","author":"Leen","year":"2007","journal-title":"J.\u00a0Immunother."},{"key":"10.1016\/j.omtm.2024.101192_bib27","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1007\/s13258-022-01261-w","article-title":"Comprehensive analysis of TCR repertoire of COVID-19 patients in different infected stage","volume":"44","author":"Wang","year":"2022","journal-title":"Genes Genomics"},{"key":"10.1016\/j.omtm.2024.101192_bib28","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/1348-0421.12967","article-title":"Effects of immune checkpoint blockade on antigen-specific CD8","volume":"66","author":"Ogando-Rivas","year":"2022","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.omtm.2024.101192_bib29","doi-asserted-by":"crossref","first-page":"5689","DOI":"10.1158\/1078-0432.CCR-20-1065","article-title":"Immune Escape After Adoptive T-cell Therapy for Malignant Gliomas","volume":"26","author":"Wildes","year":"2020","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.omtm.2024.101192_bib30","doi-asserted-by":"crossref","first-page":"1898","DOI":"10.1158\/1078-0432.CCR-16-2057","article-title":"Long-term Survival in Glioblastoma with Cytomegalovirus pp65-Targeted Vaccination","volume":"23","author":"Batich","year":"2017","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.omtm.2024.101192_bib31","doi-asserted-by":"crossref","first-page":"vi93","DOI":"10.1093\/neuonc\/now212.389","article-title":"Enhanced T\u00a0cell activation using dendritic cells pulsed with chimeric RNAs encoding full-length LAMP-1 fusion constructs","volume":"18","author":"Yang","year":"2016","journal-title":"Neuro Oncol."},{"key":"10.1016\/j.omtm.2024.101192_bib32","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1038\/nature14320","article-title":"Tetanus toxoid and CCL3 improve dendritic cell vaccines in mice and glioblastoma patients","volume":"519","author":"Mitchell","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.omtm.2024.101192_bib33","doi-asserted-by":"crossref","DOI":"10.3390\/cells11121974","article-title":"CAR T Cell Locomotion in Solid Tumor Microenvironment","volume":"11","author":"Nguyen","year":"2022","journal-title":"Cells"},{"key":"10.1016\/j.omtm.2024.101192_bib34","series-title":"mRNA Aggregates Harness Danger Response for Potent Cancer Immunotherapy","author":"Mendez-Gomez","year":"2023"},{"key":"10.1016\/j.omtm.2024.101192_bib35","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a041390","article-title":"The Evolution and Biology of SARS-CoV-2 Variants","volume":"12","author":"Telenti","year":"2022","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"10.1016\/j.omtm.2024.101192_bib36","doi-asserted-by":"crossref","first-page":"2905","DOI":"10.1182\/blood.2020008488","article-title":"SARS-CoV-2-specific T\u00a0cells are rapidly expanded for therapeutic use and target conserved regions of the membrane protein","volume":"136","author":"Keller","year":"2020","journal-title":"Blood"},{"key":"10.1016\/j.omtm.2024.101192_bib37","doi-asserted-by":"crossref","first-page":"1934","DOI":"10.3324\/haematol.2022.281947","article-title":"Longitudinal analysis of the evolution of cellular immunity to SARS-CoV-2 induced by infection and vaccination","volume":"108","author":"Vasileiou","year":"2023","journal-title":"Haematologica"},{"key":"10.1016\/j.omtm.2024.101192_bib38","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.3324\/haematol.2022.281946","article-title":"Allogeneic, Off-the-Shelf, SARS-CoV-2-specific T\u00a0cells (ALVR109) for the treatment of COVID-19 in high risk patients","volume":"108","author":"Vasileiou","year":"2023","journal-title":"Haematologica"},{"key":"10.1016\/j.omtm.2024.101192_bib39","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.omtm.2018.03.003","article-title":"A New Method for Reactivating and Expanding T Cells Specific for","volume":"9","author":"Castillo","year":"2018","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"10.1016\/j.omtm.2024.101192_bib40","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1038\/mt.2014.192","article-title":"Graft versus leukemia response without graft-versus-host disease elicited by adoptively transferred multivirus-specific T-cells","volume":"23","author":"Melenhorst","year":"2015","journal-title":"Mol. Ther."},{"key":"10.1016\/j.omtm.2024.101192_bib41","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1158\/1078-0432.CCR-13-3205","article-title":"Ultra low-dose IL-2 for GVHD prophylaxis after allogeneic hematopoietic stem cell transplantation mediates expansion of regulatory T\u00a0cells without diminishing antiviral and antileukemic activity","volume":"20","author":"Kennedy-Nasser","year":"2014","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.omtm.2024.101192_bib42","doi-asserted-by":"crossref","first-page":"eabe4782","DOI":"10.1126\/sciimmunol.abe4782","article-title":"Severely ill COVID-19 patients display impaired exhaustion features in SARS-CoV-2-reactive CD8","volume":"6","author":"Kusnadi","year":"2021","journal-title":"Sci. Immunol."},{"key":"10.1016\/j.omtm.2024.101192_bib43","article-title":"Magnitude and Dynamics of the T-Cell Response to SARS-CoV-2 Infection at Both Individual and Population Levels","author":"Snyder","year":"2020","journal-title":"medRxiv"},{"key":"10.1016\/j.omtm.2024.101192_bib44","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0141561","article-title":"Multiplex Identification of Antigen-Specific T Cell Receptors Using a Combination of Immune Assays and Immune Receptor Sequencing","volume":"10","author":"Klinger","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.omtm.2024.101192_bib45","doi-asserted-by":"crossref","first-page":"6525","DOI":"10.1002\/jmv.27191","article-title":"Updated SARS-CoV-2 single nucleotide variants and mortality association","volume":"93","author":"Fang","year":"2021","journal-title":"J.\u00a0Med. Virol."},{"key":"10.1016\/j.omtm.2024.101192_bib46","article-title":"Big data in the healthcare system: a synergy with artificial intelligence and blockchain technology","volume":"19","author":"Pablo","year":"2021","journal-title":"J.\u00a0Integr. Bioinform."},{"key":"10.1016\/j.omtm.2024.101192_bib47","article-title":"Slow-Cycling Cells in Glioblastoma: A Specific Population in the Cellular Mosaic of Cancer Stem Cells","volume":"14","author":"Yang","year":"2022","journal-title":"Cancers"},{"key":"10.1016\/j.omtm.2024.101192_bib48","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: a flexible suite of utilities for comparing genomic features","volume":"26","author":"Quinlan","year":"2010","journal-title":"Bioinformatics"}],"container-title":["Molecular Therapy - Methods &amp; Clinical Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2329050124000081?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2329050124000081?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T13:13:44Z","timestamp":1722086024000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2329050124000081"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["S2329050124000081"],"URL":"https:\/\/doi.org\/10.1016\/j.omtm.2024.101192","relation":{},"ISSN":["2329-0501"],"issn-type":[{"value":"2329-0501","type":"print"}],"subject":[],"published":{"date-parts":[[2024,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Expanded specific T\u00a0cells to hypomutated regions of the SARS-CoV-2 using mRNA electroporated antigen-presenting cells","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy - Methods & Clinical Development","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.omtm.2024.101192","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors.","name":"copyright","label":"Copyright"}],"article-number":"101192"}}