{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:19:45Z","timestamp":1792642785866,"version":"4.5.0"},"reference-count":55,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019522","name":"JCR Pharmaceuticals Co Ltd","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019522","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy Methods &amp; Clinical Development"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.omtm.2025.101474","type":"journal-article","created":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T11:15:25Z","timestamp":1745061325000},"page":"101474","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"title":["Evaluation of the loading capacity and patterns of packaged DNA in AAV genomes of different sizes using long-read sequencing"],"prefix":"10.1016","volume":"33","author":[{"given":"Mitsuki","family":"Kosaka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7474-9194","authenticated-orcid":false,"given":"Ai Fujita","family":"Sajiki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhisa","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenichi","family":"Kawano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiichi","family":"Hirazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuyoshi","family":"Matsuno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiko","family":"Takada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuhiro","family":"Shimozawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken-ichi","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenya","family":"Yuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji M.","family":"Nishiguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.omtm.2025.101474_bib1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1093\/bmb\/ldy005","article-title":"Retinal gene therapy","volume":"126","author":"Kumaran","year":"2018","journal-title":"Br. Med. Bull."},{"key":"10.1016\/j.omtm.2025.101474_bib2","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.84065","article-title":"Allele-specific gene-editing approach for vision loss restoration in RHO-associated retinitis pigmentosa","volume":"12","author":"Liu","year":"2023","journal-title":"Elife"},{"key":"10.1016\/j.omtm.2025.101474_bib3","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1007\/s10384-023-00975-y","article-title":"Genotype and phenotype characteristics of RHO-associated retinitis pigmentosa in the Japanese population","volume":"67","author":"Sakai","year":"2023","journal-title":"Jpn. J. Ophthalmol."},{"key":"10.1016\/j.omtm.2025.101474_bib4","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1038\/mt.2009.255","article-title":"Effect of genome size on AAV vector packaging","volume":"18","author":"Wu","year":"2010","journal-title":"Mol. Ther."},{"key":"10.1016\/j.omtm.2025.101474_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtm.2024.101188","article-title":"Recombinant AAV genome size effect on viral vector production, purification, and thermostability","volume":"32","author":"Ibreljic","year":"2024","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"10.1016\/j.omtm.2025.101474_bib6","doi-asserted-by":"crossref","first-page":"2101","DOI":"10.1089\/hum.1996.7.17-2101","article-title":"Quantitative analysis of the packaging capacity of recombinant adeno-associated virus","volume":"7","author":"Dong","year":"1996","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.omtm.2025.101474_bib7","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/s40259-017-0234-5","article-title":"Adeno-associated virus (AAV) as a vector for gene therapy","volume":"31","author":"Naso","year":"2017","journal-title":"BioDrugs"},{"key":"10.1016\/j.omtm.2025.101474_bib8","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1038\/s41467-019-14181-3","article-title":"Single AAV-mediated mutation replacement genome editing in limited number of photoreceptors restores vision in mice","volume":"11","author":"Nishiguchi","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omtm.2025.101474_bib9","doi-asserted-by":"crossref","first-page":"6267","DOI":"10.1038\/s41467-021-26518-y","article-title":"Self-inactivating, all-in-one AAV vectors for precision Cas9 genome editing via homology-directed repair in vivo","volume":"12","author":"Ibraheim","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omtm.2025.101474_bib10","doi-asserted-by":"crossref","first-page":"3075","DOI":"10.1038\/ncomms4075","article-title":"Drawing a high-resolution functional map of adeno-associated virus capsid by massively parallel sequencing","volume":"5","author":"Adachi","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omtm.2025.101474_bib11","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1089\/hgtb.2018.037","article-title":"Accurate and rapid sequence analysis of adeno-associated virus plasmids by Illumina next-generation sequencing","volume":"29","author":"Saveliev","year":"2018","journal-title":"Hum. Gene Ther. Methods"},{"key":"10.1016\/j.omtm.2025.101474_bib12","doi-asserted-by":"crossref","DOI":"10.1093\/gigascience\/giaa105","article-title":"NanoGalaxy: Nanopore long-read sequencing data analysis in Galaxy","volume":"9","author":"de Koning","year":"2020","journal-title":"GigaScience"},{"key":"10.1016\/j.omtm.2025.101474_bib13","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1038\/nbt.3423","article-title":"Three decades of nanopore sequencing","volume":"34","author":"Deamer","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.omtm.2025.101474_bib14","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1186\/s13059-016-1103-0","article-title":"The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community","volume":"17","author":"Jain","year":"2016","journal-title":"Genome Biol."},{"key":"10.1016\/j.omtm.2025.101474_bib15","first-page":"1187","article-title":"Direct ITR-to-ITR nanopore sequencing of AAV vector genomes","volume":"33","author":"Namkung","year":"2022","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.omtm.2025.101474_bib16","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s00408-023-00612-y","article-title":"Nanopore-based metagenomic sequencing in respiratory tract infection: A developing diagnostic platform","volume":"201","author":"Chapman","year":"2023","journal-title":"Lung"},{"key":"10.1016\/j.omtm.2025.101474_bib17","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1038\/s41587-021-01108-x","article-title":"Nanopore sequencing technology, bioinformatics and applications","volume":"39","author":"Wang","year":"2021","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.omtm.2025.101474_bib18","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1093\/jac\/dkw397","article-title":"Identification of bacterial pathogens and antimicrobial resistance directly from clinical urines by nanopore-based metagenomic sequencing","volume":"72","author":"Schmidt","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.omtm.2025.101474_bib19","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/s41587-019-0156-5","article-title":"Nanopore metagenomics enables rapid clinical diagnosis of bacterial lower respiratory infection","volume":"37","author":"Charalampous","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.omtm.2025.101474_bib20","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0259277","article-title":"Nanopore sequencing of SARS-CoV-2: Comparison of short and long PCR-tiling amplicon protocols","volume":"16","author":"Brejov\u00e1","year":"2021","journal-title":"PLoS One"},{"key":"10.1016\/j.omtm.2025.101474_bib21","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1186\/s12985-020-01454-3","article-title":"Evaluation on the use of Nanopore sequencing for direct characterization of coronaviruses from respiratory specimens, and a study on emerging missense mutations in partial RdRP gene of SARS-CoV-2","volume":"17","author":"Chan","year":"2020","journal-title":"Virol. J."},{"key":"10.1016\/j.omtm.2025.101474_bib22","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1038\/nbt.4060","article-title":"Nanopore sequencing and assembly of a human genome with ultra-long reads","volume":"36","author":"Jain","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.omtm.2025.101474_bib23","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.1093\/hmg\/8.11.2121","article-title":"Mutations in the RP1 gene causing autosomal dominant retinitis pigmentosa","volume":"8","author":"Bowne","year":"1999","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.omtm.2025.101474_bib24","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/10305","article-title":"Mutations in a gene encoding a new oxygen-regulated photoreceptor protein cause dominant retinitis pigmentosa","volume":"22","author":"Pierce","year":"1999","journal-title":"Nat. Genet."},{"key":"10.1016\/j.omtm.2025.101474_bib25","first-page":"4069","article-title":"RP1 protein truncating mutations predominate at the RP1 adRP locus","volume":"41","author":"Payne","year":"2000","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.omtm.2025.101474_bib26","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1093\/hmg\/8.8.1541","article-title":"A nonsense mutation in a novel gene is associated with retinitis pigmentosa in a family linked to the RP1 locus","volume":"8","author":"Guillonneau","year":"1999","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.omtm.2025.101474_bib27","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.3002103","article-title":"Dosage thresholds for AAV2 and AAV8 photoreceptor gene therapy in monkey","volume":"3","author":"Vandenberghe","year":"2011","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.omtm.2025.101474_bib28","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1089\/hum.2016.111","article-title":"Evaluation of dose and safety of AAV7m8 and AAV8BP2 in the non-human primate retina","volume":"28","author":"Ramachandran","year":"2017","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.omtm.2025.101474_bib29","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1089\/hum.2018.144","article-title":"Dosage thresholds and influence of transgene cassette in adeno-associated virus-related toxicity","volume":"29","author":"Khabou","year":"2018","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.omtm.2025.101474_bib30","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1182\/blood-2013-01-306647","article-title":"Immune responses to AAV vectors: overcoming barriers to successful gene therapy","volume":"122","author":"Mingozzi","year":"2013","journal-title":"Blood"},{"key":"10.1016\/j.omtm.2025.101474_bib31","doi-asserted-by":"crossref","DOI":"10.1038\/mtm.2015.9","article-title":"A comparison of AAV strategies distinguishes overlapping vectors for efficient systemic delivery of the 6.2 kb Dysferlin coding sequence","volume":"2","author":"Pryadkina","year":"2015","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"10.1016\/j.omtm.2025.101474_bib32","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1038\/mt.2009.258","article-title":"Characterization of genome integrity for oversized recombinant AAV vector","volume":"18","author":"Dong","year":"2010","journal-title":"Mol. Ther."},{"key":"10.1016\/j.omtm.2025.101474_bib33","doi-asserted-by":"crossref","first-page":"9451","DOI":"10.1128\/JVI.74.20.9451-9463.2000","article-title":"Recruitment of single-stranded recombinant adeno-associated virus vector genomes and intermolecular recombination are responsible for stable transduction of liver in vivo","volume":"74","author":"Nakai","year":"2000","journal-title":"J. Virol."},{"key":"10.1016\/j.omtm.2025.101474_bib34","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.1038\/mt.2013.184","article-title":"Oversized AAV transductifon is mediated via a DNA-PKcs-independent, Rad51C-dependent repair pathway","volume":"21","author":"Hirsch","year":"2013","journal-title":"Mol. Ther."},{"key":"10.1016\/j.omtm.2025.101474_bib35","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1038\/nbt0702-697","article-title":"Efficient mouse airway transduction following recombination between AAV vectors carrying parts of a larger gene","volume":"20","author":"Halbert","year":"2002","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.omtm.2025.101474_bib36","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/j.omtm.2020.07.007","article-title":"AAV-genome population sequencing of vectors packaging CRISPR components reveals design-influenced heterogeneity","volume":"18","author":"Tran","year":"2020","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"10.1016\/j.omtm.2025.101474_bib37","doi-asserted-by":"crossref","first-page":"9468","DOI":"10.1128\/JVI.73.11.9468-9477.1999","article-title":"Concatamerization of adeno-associated virus circular genomes occurs through intermolecular recombination","volume":"73","author":"Yang","year":"1999","journal-title":"J. Virol."},{"key":"10.1016\/j.omtm.2025.101474_bib38","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1089\/10430340360464697","article-title":"Efficient trans-splicing in the retina expands the utility of adeno-associated virus as a vector for gene therapy","volume":"14","author":"Reich","year":"2003","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.omtm.2025.101474_bib39","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1006\/mthe.2001.0456","article-title":"Expanding AAV packaging capacity with trans-splicing or overlapping vectors: a quantitative comparison","volume":"4","author":"Duan","year":"2001","journal-title":"Mol. Ther."},{"key":"10.1016\/j.omtm.2025.101474_bib40","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1002\/emmm.201302948","article-title":"Effective delivery of large genes to the retina by dual AAV vectors","volume":"6","author":"Trapani","year":"2014","journal-title":"EMBO Mol. Med."},{"key":"10.1016\/j.omtm.2025.101474_bib41","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1038\/75080","article-title":"A new dual-vector approach to enhance recombinant adeno-associated virus-mediated gene expression through intermolecular cis activation","volume":"6","author":"Duan","year":"2000","journal-title":"Nat. Med."},{"key":"10.1016\/j.omtm.2025.101474_bib42","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1038\/sj.mt.6300322","article-title":"A hybrid vector system expands adeno-associated viral vector packaging capacity in a transgene-independent manner","volume":"16","author":"Ghosh","year":"2008","journal-title":"Mol. Ther."},{"key":"10.1016\/j.omtm.2025.101474_bib43","doi-asserted-by":"crossref","first-page":"3781","DOI":"10.1016\/S0021-9258(18)53762-5","article-title":"Expression of the cystic fibrosis transmembrane conductance regulator from a novel adeno-associated virus promoter","volume":"268","author":"Flotte","year":"1993","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.omtm.2025.101474_bib44","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1089\/hum.2019.274","article-title":"Adeno-associated virus serotype-specific inverted terminal repeat sequence role in vector transgene expression","volume":"31","author":"Earley","year":"2020","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.omtm.2025.101474_bib45","doi-asserted-by":"crossref","DOI":"10.1038\/srep18141","article-title":"In vivo cellular imaging of various stress\/response pathways using AAV following axonal injury in mice","volume":"5","author":"Fujita","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.omtm.2025.101474_bib46","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1038\/nbt0102-87","article-title":"A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications","volume":"20","author":"Nagai","year":"2002","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.omtm.2025.101474_bib47","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.omtm.2017.04.003","article-title":"Spatially and temporally regulated NRF2 gene therapy using Mcp-1 promoter in retinal ganglion cell injury","volume":"5","author":"Fujita","year":"2017","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"10.1016\/j.omtm.2025.101474_bib48","doi-asserted-by":"crossref","first-page":"3094","DOI":"10.1093\/bioinformatics\/bty191","article-title":"Minimap2: pairwise alignment for nucleotide sequences","volume":"34","author":"Li","year":"2018","journal-title":"Bioinformatics"},{"key":"10.1016\/j.omtm.2025.101474_bib49","doi-asserted-by":"crossref","first-page":"4572","DOI":"10.1093\/bioinformatics\/btab705","article-title":"New strategies to improve minimap2 alignment accuracy","volume":"37","author":"Li","year":"2021","journal-title":"Bioinformatics"},{"key":"10.1016\/j.omtm.2025.101474_bib50","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/nbt.1754","article-title":"Integrative genomics viewer","volume":"29","author":"Robinson","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.omtm.2025.101474_bib51","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1093\/bib\/bbs017","article-title":"Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration","volume":"14","author":"Thorvaldsd\u00f3ttir","year":"2013","journal-title":"Brief. Bioinform."},{"key":"10.1016\/j.omtm.2025.101474_bib52","doi-asserted-by":"crossref","first-page":"e31","DOI":"10.1158\/0008-5472.CAN-17-0337","article-title":"Variant review with the Integrative Genomics Viewer","volume":"77","author":"Robinson","year":"2017","journal-title":"Cancer Res."},{"key":"10.1016\/j.omtm.2025.101474_bib53","doi-asserted-by":"crossref","DOI":"10.1093\/bioinformatics\/btac830","article-title":"igv.js: an embeddable JavaScript implementation of the Integrative Genomics Viewer (IGV)","volume":"39","author":"Robinson","year":"2023","journal-title":"Bioinformatics"},{"key":"10.1016\/j.omtm.2025.101474_bib54","doi-asserted-by":"crossref","DOI":"10.1016\/j.redox.2023.102890","article-title":"Retinal ferroptosis as a critical mechanism for the induction of retinochoroiditis during ocular toxoplasmosis","volume":"67","author":"Yamada","year":"2023","journal-title":"Redox Biol."},{"key":"10.1016\/j.omtm.2025.101474_bib55","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/s41591-018-0327-9","article-title":"Development of a gene-editing approach to restore vision loss in Leber congenital amaurosis type 10","volume":"25","author":"Maeder","year":"2019","journal-title":"Nat. Med."}],"container-title":["Molecular Therapy Methods &amp; Clinical Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2329050125000695?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2329050125000695?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T18:51:59Z","timestamp":1759863119000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2329050125000695"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":55,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["S2329050125000695"],"URL":"https:\/\/doi.org\/10.1016\/j.omtm.2025.101474","relation":{},"ISSN":["2329-0501"],"issn-type":[{"value":"2329-0501","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Evaluation of the loading capacity and patterns of packaged DNA in AAV genomes of different sizes using long-read sequencing","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy Methods & Clinical Development","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.omtm.2025.101474","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc. on behalf of The American Society of Gene and Cell Therapy.","name":"copyright","label":"Copyright"}],"article-number":"101474"}}