{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T21:07:15Z","timestamp":1781644035082,"version":"3.54.5"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T00:00:00Z","timestamp":1582588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005150","name":"Chinese Academy of Medical Sciences","doi-asserted-by":"publisher","award":["2019-RC-HL-019"],"award-info":[{"award-number":["2019-RC-HL-019"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470649"],"award-info":[{"award-number":["81470649"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670870"],"award-info":[{"award-number":["81670870"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2012CB945101"],"award-info":[{"award-number":["2012CB945101"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2011CB510200"],"award-info":[{"award-number":["2011CB510200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005090","name":"Beijing Nova Program","doi-asserted-by":"publisher","award":["Z161100004916058"],"award-info":[{"award-number":["Z161100004916058"]}],"id":[{"id":"10.13039\/501100005090","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy Nucleic Acids"],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.1016\/j.omtn.2020.02.010","type":"journal-article","created":{"date-parts":[[2020,2,27]],"date-time":"2020-02-27T21:41:07Z","timestamp":1582839667000},"page":"217-230","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["ube3d, a New Gene Associated with Age-Related Macular Degeneration, Induces Functional Changes in Both In\u00a0Vivo and In\u00a0Vitro Studies"],"prefix":"10.1016","volume":"20","author":[{"given":"Huika","family":"Xia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujing","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinrui","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lvzhen","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.omtn.2020.02.010_bib1","first-page":"844","article-title":"Global data on visual impairment in the year 2002","volume":"82","author":"Resnikoff","year":"2004","journal-title":"Bull. World Health Organ."},{"key":"10.1016\/j.omtn.2020.02.010_bib2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1076\/opep.11.2.67.28158","article-title":"2002 global update of available data on visual impairment: a compilation of population-based prevalence studies","volume":"11","author":"Pascolini","year":"2004","journal-title":"Ophthalmic Epidemiol."},{"key":"10.1016\/j.omtn.2020.02.010_bib3","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1056\/NEJMoa054481","article-title":"Ranibizumab for neovascular age-related macular degeneration","volume":"355","author":"Rosenfeld","year":"2006","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.omtn.2020.02.010_bib4","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1007\/s00417-003-0812-8","article-title":"Ageing of the retinal pigment epithelium: implications for transplantation","volume":"242","author":"Boulton","year":"2004","journal-title":"Graefes Arch. Clin. Exp. Ophthalmol."},{"key":"10.1016\/j.omtn.2020.02.010_bib5","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1001\/archopht.122.4.598","article-title":"Current concepts in the pathogenesis of age-related macular degeneration","volume":"122","author":"Zarbin","year":"2004","journal-title":"Arch. Ophthalmol."},{"key":"10.1016\/j.omtn.2020.02.010_bib6","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.exer.2004.11.009","article-title":"Mechanisms for the induction of HNE- MDA- and AGE-adducts, RAGE and VEGF in retinal pigment epithelial cells","volume":"80","author":"Zhou","year":"2005","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.omtn.2020.02.010_bib7","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1038\/nri3459","article-title":"Immunology of age-related macular degeneration","volume":"13","author":"Ambati","year":"2013","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.omtn.2020.02.010_bib8","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1042\/bse0380051","article-title":"The ubiquitin-proteasome pathway of intracellular proteolysis","volume":"38","author":"Doherty","year":"2002","journal-title":"Essays Biochem."},{"key":"10.1016\/j.omtn.2020.02.010_bib9","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1038\/77060","article-title":"Familial Parkinson disease gene product, parkin, is a ubiquitin-protein ligase","volume":"25","author":"Shimura","year":"2000","journal-title":"Nat. Genet."},{"key":"10.1016\/j.omtn.2020.02.010_bib10","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1096\/fasebj.11.14.9409544","article-title":"Pathways of ubiquitin conjugation","volume":"11","author":"Haas","year":"1997","journal-title":"FASEB J."},{"key":"10.1016\/j.omtn.2020.02.010_bib11","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1096\/fj.09-136259","article-title":"The family of ubiquitin-conjugating enzymes (E2s): deciding between life and death of proteins","volume":"24","author":"van Wijk","year":"2010","journal-title":"FASEB J."},{"key":"10.1016\/j.omtn.2020.02.010_bib12","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/0304-3940(88)90297-2","article-title":"Ubiquitin is a component of neurofibrillary tangles in a variety of neurodegenerative diseases","volume":"94","author":"Lennox","year":"1988","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.omtn.2020.02.010_bib13","doi-asserted-by":"crossref","first-page":"9902","DOI":"10.1073\/pnas.170173897","article-title":"Inhibition of the ubiquitin-proteasome system in Alzheimer\u2019s disease","volume":"97","author":"Lam","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.omtn.2020.02.010_bib14","doi-asserted-by":"crossref","first-page":"6687","DOI":"10.1038\/ncomms7687","article-title":"Whole-exome sequencing implicates UBE3D in age-related macular degeneration in East Asian populations","volume":"6","author":"Huang","year":"2015","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omtn.2020.02.010_bib15","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1002\/(SICI)1096-9861(19990222)404:4<515::AID-CNE8>3.0.CO;2-A","article-title":"Early retinal development in the zebrafish, Danio rerio: light and electron microscopic analyses","volume":"404","author":"Schmitt","year":"1999","journal-title":"J.\u00a0Comp. Neurol."},{"key":"10.1016\/j.omtn.2020.02.010_bib16","doi-asserted-by":"crossref","first-page":"14682","DOI":"10.1073\/pnas.222551899","article-title":"Drusen proteome analysis: an approach to the etiology of age-related macular degeneration","volume":"99","author":"Crabb","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.omtn.2020.02.010_bib17","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.mam.2012.04.001","article-title":"Roles for the ubiquitin-proteasome pathway in protein quality control and signaling in the retina: implications in the pathogenesis of age-related macular degeneration","volume":"33","author":"Shang","year":"2012","journal-title":"Mol. Aspects Med."},{"key":"10.1016\/j.omtn.2020.02.010_bib18","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.neuron.2010.01.017","article-title":"E3 ligase Nedd4 promotes axon branching by downregulating PTEN","volume":"65","author":"Drinjakovic","year":"2010","journal-title":"Neuron"},{"key":"10.1016\/j.omtn.2020.02.010_bib19","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1006\/dbio.2002.0736","article-title":"Activated MAPK\/ERK kinase (MEK-1) induces transdifferentiation of pigmented epithelium into neural retina","volume":"248","author":"Galy","year":"2002","journal-title":"Dev. Biol."},{"key":"10.1016\/j.omtn.2020.02.010_bib20","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1006\/excr.2000.4803","article-title":"Regulation of microphthalmia-associated transcription factor MITF protein levels by association with the ubiquitin-conjugating enzyme hUBC9","volume":"255","author":"Xu","year":"2000","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.omtn.2020.02.010_bib21","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/0896-6273(93)90182-Q","article-title":"The Drosophila bendless gene encodes a neural protein related to ubiquitin-conjugating enzymes","volume":"11","author":"Muralidhar","year":"1993","journal-title":"Neuron"},{"key":"10.1016\/j.omtn.2020.02.010_bib22","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1038\/ncb799","article-title":"The DIAP1 RING finger mediates ubiquitination of Dronc and is indispensable for regulating apoptosis","volume":"4","author":"Wilson","year":"2002","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.omtn.2020.02.010_bib23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1749-6632.1998.tb10102.x","article-title":"Morphogenesis of the retinal pigment epithelium: toward understanding retinal degenerative diseases","volume":"857","author":"Marmorstein","year":"1998","journal-title":"Ann. N Y Acad. Sci."},{"key":"10.1016\/j.omtn.2020.02.010_bib24","first-page":"1057","article-title":"Polarity and the development of the outer blood-retinal barrier","volume":"12","author":"Rizzolo","year":"1997","journal-title":"Histol. Histopathol."},{"key":"10.1016\/j.omtn.2020.02.010_bib25","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1038\/nrn2283","article-title":"Evolution of the vertebrate eye: opsins, photoreceptors, retina and eye cup","volume":"8","author":"Lamb","year":"2007","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1016\/j.omtn.2020.02.010_bib26","first-page":"397","article-title":"Outer segment disc shedding and phagocytosis in the outer retina","volume":"103","author":"LaVail","year":"1983","journal-title":"Trans. Ophthalmol. Soc. U. K."},{"key":"10.1016\/j.omtn.2020.02.010_bib27","first-page":"8","article-title":"Phagocytosis of retinal rod and cone photoreceptors","volume":"25","author":"Kevany","year":"2010","journal-title":"Physiology (Bethesda)"},{"key":"10.1016\/j.omtn.2020.02.010_bib28","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/978-1-4614-3209-8_31","article-title":"Impairment of the ubiquitin-proteasome pathway in RPE alters the expression of inflammation related genes","volume":"801","author":"Liu","year":"2014","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.omtn.2020.02.010_bib29","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/j.1600-0749.2004.00138.x","article-title":"The melanosome as a model to study organelle motility in mammals","volume":"17","author":"Barral","year":"2004","journal-title":"Pigment Cell Res."},{"key":"10.1016\/j.omtn.2020.02.010_bib30","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1042\/BST0391191","article-title":"Melanosomes on the move: a model to understand organelle dynamics","volume":"39","author":"Hume","year":"2011","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.omtn.2020.02.010_bib31","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1034\/j.1600-0749.2003.00034.x","article-title":"The Seiji memorial lecture: the melanosome: an ideal model to study cellular differentiation","volume":"16","author":"Kushimoto","year":"2003","journal-title":"Pigment Cell Res."},{"key":"10.1016\/j.omtn.2020.02.010_bib32","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1016\/j.ophtha.2012.05.016","article-title":"Bevacizumab for neovascular age-related macular degeneration in China","volume":"119","author":"Li","year":"2012","journal-title":"Ophthalmology"},{"key":"10.1016\/j.omtn.2020.02.010_bib33","first-page":"147","article-title":"Safety of ranibizumab therapy in wet AMD and the role of vascular endothelial growth factors in physiological angiogenesis","volume":"112","author":"Figurska","year":"2010","journal-title":"Klin. Oczna"},{"key":"10.1016\/j.omtn.2020.02.010_bib34","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.arr.2009.06.002","article-title":"Regulatory role of HIF-1alpha in the pathogenesis of age-related macular degeneration (AMD)","volume":"8","author":"Arjamaa","year":"2009","journal-title":"Ageing Res. Rev."},{"key":"10.1016\/j.omtn.2020.02.010_bib35","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.1016\/j.exer.2006.07.024","article-title":"Proteasome-dependent regulation of signal transduction in retinal pigment epithelial cells","volume":"83","author":"Fernandes","year":"2006","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.omtn.2020.02.010_bib36","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1161\/CIRCRESAHA.1134303145","article-title":"Fli1 acts downstream of Etv2 to govern cell survival and vascular homeostasis via positive autoregulation","volume":"114","author":"Abedin","year":"2014","journal-title":"Circ. Res."},{"key":"10.1016\/j.omtn.2020.02.010_bib37","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1096\/fasebj.13.1.9","article-title":"Vascular endothelial growth factor (VEGF) and its receptors","volume":"13","author":"Neufeld","year":"1999","journal-title":"FASEB J."},{"key":"10.1016\/j.omtn.2020.02.010_bib38","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1038\/sj.onc.1207973","article-title":"The anaphase-promoting complex: a key factor in the regulation of cell cycle","volume":"24","author":"Castro","year":"2005","journal-title":"Oncogene"},{"key":"10.1016\/j.omtn.2020.02.010_bib39","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S1350-9462(99)00009-9","article-title":"Oxidative damage and protection of the RPE","volume":"19","author":"Cai","year":"2000","journal-title":"Prog. Retin. Eye Res."},{"key":"10.1016\/j.omtn.2020.02.010_bib40","first-page":"32","article-title":"Oxidative damage and age-related macular degeneration","volume":"5","author":"Winkler","year":"1999","journal-title":"Mol. Vis."},{"key":"10.1016\/j.omtn.2020.02.010_bib41","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1007\/s12035-012-8391-5","article-title":"The ubiquitin-proteasome system in retinal health and disease","volume":"47","author":"Campello","year":"2013","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.omtn.2020.02.010_bib42","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1006\/exer.1996.0176","article-title":"Age-related decline in ubiquitin conjugation in response to oxidative stress in the lens","volume":"64","author":"Shang","year":"1997","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.omtn.2020.02.010_bib43","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.2741\/e198","article-title":"Mechanisms of protein aggregation in the retinal pigment epithelial cells","volume":"2","author":"Kaarniranta","year":"2010","journal-title":"Front. Biosci. (Elite Ed.)"},{"key":"10.1016\/j.omtn.2020.02.010_bib44","first-page":"1399","article-title":"p62\/sequestosome 1 as a regulator of proteasome inhibitor-induced autophagy in human retinal pigment epithelial cells","volume":"16","author":"Viiri","year":"2010","journal-title":"Mol. Vis."},{"key":"10.1016\/j.omtn.2020.02.010_bib45","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1038\/ncb2012","article-title":"PINK1\/Parkin-mediated mitophagy is dependent on VDAC1 and p62\/SQSTM1","volume":"12","author":"Geisler","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.omtn.2020.02.010_bib46","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1242\/dev.119.4.1203","article-title":"Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos","volume":"119","author":"Thisse","year":"1993","journal-title":"Development"},{"key":"10.1016\/j.omtn.2020.02.010_bib47","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1093\/hmg\/ddi468","article-title":"Bardet-Biedl syndrome genes are important in retrograde intracellular trafficking and Kupffer\u2019s vesicle cilia function","volume":"15","author":"Yen","year":"2006","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.omtn.2020.02.010_bib48","doi-asserted-by":"crossref","first-page":"6628","DOI":"10.1167\/iovs.13-12625","article-title":"Effects of semaphorin 3A on retinal pigment epithelial cell activity","volume":"54","author":"Bai","year":"2013","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.omtn.2020.02.010_bib49","doi-asserted-by":"crossref","first-page":"3193","DOI":"10.1167\/iovs.09-3779","article-title":"Effect of Robo1 on retinal pigment epithelial cells and experimental proliferative vitreoretinopathy","volume":"51","author":"Huang","year":"2010","journal-title":"Invest. Ophthalmol. Vis. Sci."}],"container-title":["Molecular Therapy Nucleic Acids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2162253120300871?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2162253120300871?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T09:52:47Z","timestamp":1778838767000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2162253120300871"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":49,"alternative-id":["S2162253120300871"],"URL":"https:\/\/doi.org\/10.1016\/j.omtn.2020.02.010","relation":{},"ISSN":["2162-2531"],"issn-type":[{"value":"2162-2531","type":"print"}],"subject":[],"published":{"date-parts":[[2020,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"ube3d, a New Gene Associated with Age-Related Macular Degeneration, Induces Functional Changes in Both In\u00a0Vivo and In\u00a0Vitro Studies","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy Nucleic Acids","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.omtn.2020.02.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Author(s).","name":"copyright","label":"Copyright"}]}}