{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T02:19:42Z","timestamp":1793413182247,"version":"4.5.0"},"reference-count":78,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T00:00:00Z","timestamp":1641772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010040","name":"Taishan Scholar Project of Shandong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010040","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015507","name":"Shandong First Medical University","doi-asserted-by":"publisher","award":["2019RC008"],"award-info":[{"award-number":["2019RC008"]}],"id":[{"id":"10.13039\/501100015507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015507","name":"Shandong First Medical University","doi-asserted-by":"publisher","award":["2019ZL001"],"award-info":[{"award-number":["2019ZL001"]}],"id":[{"id":"10.13039\/501100015507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81800876"],"award-info":[{"award-number":["81800876"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101092"],"award-info":[{"award-number":["82101092"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010029","name":"Taishan Scholar Foundation of Shandong Province","doi-asserted-by":"publisher","award":["20150215"],"award-info":[{"award-number":["20150215"]}],"id":[{"id":"10.13039\/501100010029","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010029","name":"Taishan Scholar Foundation of Shandong Province","doi-asserted-by":"publisher","award":["20161059"],"award-info":[{"award-number":["20161059"]}],"id":[{"id":"10.13039\/501100010029","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2019BH078"],"award-info":[{"award-number":["ZR2019BH078"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2019ZD37"],"award-info":[{"award-number":["ZR2019ZD37"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy Nucleic Acids"],"published-print":{"date-parts":[[2022,3]]},"DOI":"10.1016\/j.omtn.2022.01.005","type":"journal-article","created":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T12:13:46Z","timestamp":1641816826000},"page":"880-893","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":42,"special_numbering":"C","title":["Heterogeneity of human corneal endothelium implicates lncRNA NEAT1 in Fuchs endothelial corneal dystrophy"],"prefix":"10.1016","volume":"27","author":[{"given":"Qun","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengqian","family":"Dou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyun","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxiao","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bi Ning","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixin","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihai","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingjun","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiyun","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.omtn.2022.01.005_bib1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.exer.2003.08.002","article-title":"Clinical responses of the corneal endothelium","volume":"78","author":"Bourne","year":"2004","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.omtn.2022.01.005_bib2","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S1350-9462(02)00065-4","article-title":"Proliferative capacity of the corneal endothelium","volume":"22","author":"Joyce","year":"2003","journal-title":"Prog. Retin. Eye Res."},{"key":"10.1016\/j.omtn.2022.01.005_bib3","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.exer.2011.08.014","article-title":"Proliferative capacity of corneal endothelial cells","volume":"95","author":"Joyce","year":"2012","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.omtn.2022.01.005_bib4","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1016\/S0140-6736(12)60437-1","article-title":"Corneal transplantation","volume":"379","author":"Tan","year":"2012","journal-title":"Lancet (London, England)"},{"key":"10.1016\/j.omtn.2022.01.005_bib5","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1038\/nmeth.1315","article-title":"mRNA-Seq whole-transcriptome analysis of a single cell","volume":"6","author":"Tang","year":"2009","journal-title":"Nat. Methods"},{"key":"10.1016\/j.omtn.2022.01.005_bib6","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1016\/j.cell.2016.07.054","article-title":"Comprehensive classification of retinal bipolar neurons by single-cell transcriptomics","volume":"166","author":"Shekhar","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.omtn.2022.01.005_bib7","doi-asserted-by":"crossref","first-page":"e3000365","DOI":"10.1371\/journal.pbio.3000365","article-title":"Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis","volume":"17","author":"Hu","year":"2019","journal-title":"PLoS Biol."},{"key":"10.1016\/j.omtn.2022.01.005_bib8","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1016\/j.cell.2019.01.004","article-title":"Molecular classification and comparative taxonomics of foveal and peripheral cells in primate retina","volume":"176","author":"Peng","year":"2019","journal-title":"Cell"},{"key":"10.1016\/j.omtn.2022.01.005_bib9","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1016\/j.neuron.2019.04.010","article-title":"Single-cell RNA-seq analysis of retinal development identifies NFI factors as regulating mitotic exit and late-born cell specification","volume":"102","author":"Clark","year":"2019","journal-title":"Neuron"},{"key":"10.1016\/j.omtn.2022.01.005_bib10","doi-asserted-by":"crossref","first-page":"e100811","DOI":"10.15252\/embj.2018100811","article-title":"A single-cell transcriptome atlas of the adult human retina","volume":"38","author":"Lukowski","year":"2019","journal-title":"EMBO J."},{"key":"10.1016\/j.omtn.2022.01.005_bib11","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1038\/s41467-018-05134-3","article-title":"Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes","volume":"9","author":"Rheaume","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omtn.2022.01.005_bib12","doi-asserted-by":"crossref","first-page":"4902","DOI":"10.1038\/s41467-019-12780-8","article-title":"Single-cell transcriptomic atlas of the human retina identifies cell types associated with age-related macular degeneration","volume":"10","author":"Menon","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omtn.2022.01.005_bib13","doi-asserted-by":"crossref","first-page":"5743","DOI":"10.1038\/s41467-019-12917-9","article-title":"Single-nuclei RNA-seq on human retinal tissue provides improved transcriptome profiling","volume":"10","author":"Liang","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omtn.2022.01.005_bib14","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.1721443115","article-title":"Scleral hypoxia is a target for myopia control","volume":"115","author":"Wu","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.omtn.2022.01.005_bib15","doi-asserted-by":"crossref","first-page":"10339","DOI":"10.1073\/pnas.2001250117","article-title":"Cell atlas of aqueous humor outflow pathways in eyes of humans and four model species provides insight into glaucoma pathogenesis","volume":"117","author":"van Zyl","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.omtn.2022.01.005_bib16","doi-asserted-by":"crossref","first-page":"12856","DOI":"10.1073\/pnas.2001896117","article-title":"Molecular taxonomy of human ocular outflow tissues defined by single-cell transcriptomics","volume":"117","author":"Patel","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.omtn.2022.01.005_bib17","doi-asserted-by":"crossref","first-page":"3570","DOI":"10.1167\/iovs.19-27656","article-title":"Single-cell RNA transcriptome helps define the limbal\/corneal epithelial stem\/early transit amplifying cells and how autophagy affects this population","volume":"60","author":"Kaplan","year":"2019","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.omtn.2022.01.005_bib18","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1016\/j.stem.2021.04.003","article-title":"Discrete limbal epithelial stem cell populations mediate corneal homeostasis and wound healing","volume":"28","author":"Altshuler","year":"2021","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.omtn.2022.01.005_bib19","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.jtos.2020.12.004","article-title":"Single-cell transcriptomics identifies limbal stem cell population and cell types mapping its differentiation trajectory in limbal basal epithelium of human cornea","volume":"20","author":"Li","year":"2021","journal-title":"Ocul. Surf."},{"key":"10.1016\/j.omtn.2022.01.005_bib20","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1681\/ASN.2019080832","article-title":"Single-cell RNA sequencing reveals renal endothelium heterogeneity and metabolic adaptation to water deprivation","volume":"31","author":"Dumas","year":"2020","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.omtn.2022.01.005_bib21","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1016\/j.cell.2020.01.015","article-title":"Single-cell transcriptome atlas of murine endothelial cells","volume":"180","author":"Kalucka","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.omtn.2022.01.005_bib22","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1073\/pnas.1912546116","article-title":"Ultraviolet A light induces DNA damage and estrogen-DNA adducts in Fuchs endothelial corneal dystrophy causing females to be more affected","volume":"117","author":"Liu","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.omtn.2022.01.005_bib23","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/nbt.3192","article-title":"Spatial reconstruction of single-cell gene expression data","volume":"33","author":"Satija","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.omtn.2022.01.005_bib24","doi-asserted-by":"crossref","first-page":"4385","DOI":"10.1167\/iovs.16-19771","article-title":"Cultured human corneal endothelial cell aneuploidy dependence on the presence of heterogeneous subpopulations with distinct differentiation phenotypes","volume":"57","author":"Hamuro","year":"2016","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.omtn.2022.01.005_bib25","doi-asserted-by":"crossref","first-page":"4749","DOI":"10.1167\/iovs.16-19770","article-title":"Cell homogeneity indispensable for regenerative medicine by cultured human corneal endothelial cells","volume":"57","author":"Hamuro","year":"2016","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.omtn.2022.01.005_bib26","doi-asserted-by":"crossref","first-page":"52904","DOI":"10.1074\/jbc.M406868200","article-title":"Matrix GLA protein stimulates VEGF expression through increased transforming growth factor-beta1 activity in endothelial cells","volume":"279","author":"Bostr\u00f6m","year":"2004","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.omtn.2022.01.005_bib27","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1113\/JP276245","article-title":"Novel mechanisms regulating endothelial barrier function in the pulmonary microcirculation","volume":"597","author":"Simmons","year":"2019","journal-title":"J.\u00a0Physiol."},{"key":"10.1016\/j.omtn.2022.01.005_bib28","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1002\/ana.22318","article-title":"Glut1\/SLC2A1 is crucial for the development of the blood-brain barrier in\u00a0vivo","volume":"68","author":"Zheng","year":"2010","journal-title":"Ann. Neurol."},{"key":"10.1016\/j.omtn.2022.01.005_bib29","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.freeradbiomed.2019.12.014","article-title":"Loss of NQO1 generates genotoxic estrogen-DNA adducts in fuchs endothelial corneal dystrophy","volume":"147","author":"Miyajima","year":"2020","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.omtn.2022.01.005_bib30","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/j.exer.2005.06.012","article-title":"Cell cycle status in human corneal endothelium","volume":"81","author":"Joyce","year":"2005","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.omtn.2022.01.005_bib31","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1007\/s004030050431","article-title":"Immunohistochemical staining of proliferating cell nuclear antigen (PCNA) in malignant and nonmalignant skin diseases","volume":"291","author":"Kawahira","year":"1999","journal-title":"Arch. Dermatol. Res."},{"key":"10.1016\/j.omtn.2022.01.005_bib32","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/375421a0","article-title":"MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells","volume":"375","author":"Madine","year":"1995","journal-title":"Nature"},{"key":"10.1016\/j.omtn.2022.01.005_bib33","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.gde.2003.11.002","article-title":"MCM proteins: DNA damage, mutagenesis and repair","volume":"14","author":"Bailis","year":"2004","journal-title":"Curr. Opin. Genet. Dev."},{"key":"10.1016\/j.omtn.2022.01.005_bib34","doi-asserted-by":"crossref","first-page":"9350","DOI":"10.1128\/MCB.25.21.9350-9359.2005","article-title":"Nuclear dynamics of PCNA in DNA replication and repair","volume":"25","author":"Essers","year":"2005","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.omtn.2022.01.005_bib35","doi-asserted-by":"crossref","first-page":"3826","DOI":"10.1167\/iovs.02-0601","article-title":"Increased endothelial survival of organ-cultured corneas stored in FGF-2-supplemented serum-free medium","volume":"44","author":"Rieck","year":"2003","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.omtn.2022.01.005_bib36","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/0014-4835(77)90248-2","article-title":"Stimulation of corneal endothelial cell proliferations in\u00a0vitro by fibroblast and epidermal growth factors","volume":"25","author":"Gospodarowicz","year":"1977","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.omtn.2022.01.005_bib37","doi-asserted-by":"crossref","first-page":"e46742","DOI":"10.1371\/journal.pone.0046742","article-title":"Differing roles for TCF4 and COL8A2 in central corneal thickness and fuchs endothelial corneal dystrophy","volume":"7","author":"Igo","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.omtn.2022.01.005_bib38","doi-asserted-by":"crossref","first-page":"4304","DOI":"10.1093\/hmg\/ddq349","article-title":"New loci associated with central cornea thickness include COL5A1, AKAP13 and AVGR8","volume":"19","author":"Vitart","year":"2010","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.omtn.2022.01.005_bib39","doi-asserted-by":"crossref","first-page":"100863","DOI":"10.1016\/j.preteyeres.2020.100863","article-title":"Fuchs endothelial corneal dystrophy: the vicious cycle of Fuchs pathogenesis","volume":"80","author":"Ong Tone","year":"2021","journal-title":"Prog. Retin. Eye Res."},{"key":"10.1016\/j.omtn.2022.01.005_bib40","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.molcel.2014.01.009","article-title":"Long noncoding RNA NEAT1-dependent SFPQ relocation from promoter region to paraspeckle mediates IL8 expression upon immune stimuli","volume":"53","author":"Imamura","year":"2014","journal-title":"Mol. Cell"},{"key":"10.1016\/j.omtn.2022.01.005_bib41","doi-asserted-by":"crossref","first-page":"5383","DOI":"10.1038\/ncomms6383","article-title":"The oestrogen receptor alpha-regulated lncRNA NEAT1 is a critical modulator of prostate cancer","volume":"5","author":"Chakravarty","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omtn.2022.01.005_bib42","doi-asserted-by":"crossref","first-page":"4482","DOI":"10.1038\/onc.2014.378","article-title":"Tumor hypoxia induces nuclear paraspeckle formation through HIF-2\u03b1 dependent transcriptional activation of NEAT1 leading to cancer cell survival","volume":"34","author":"Choudhry","year":"2015","journal-title":"Oncogene"},{"key":"10.1016\/j.omtn.2022.01.005_bib43","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.1803725115","article-title":"Long noncoding RNA NEAT1 (nuclear paraspeckle assembly transcript 1) is critical for phenotypic switching of vascular smooth muscle cells","volume":"115","author":"Ahmed","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.omtn.2022.01.005_bib44","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.freeradbiomed.2020.12.445","article-title":"Cell cycle re-entry and arrest in G2\/M phase induces senescence and fibrosis in Fuchs Endothelial Corneal Dystrophy","volume":"164","author":"White","year":"2021","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.omtn.2022.01.005_bib45","doi-asserted-by":"crossref","first-page":"9291","DOI":"10.1167\/iovs.11-8312","article-title":"p53-regulated increase in oxidative-stress--induced apoptosis in Fuchs endothelial corneal dystrophy: a native tissue model","volume":"52","author":"Azizi","year":"2011","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"issue":"Suppl 1","key":"10.1016\/j.omtn.2022.01.005_bib46","doi-asserted-by":"crossref","first-page":"S50","DOI":"10.1097\/ICO.0000000000001775","article-title":"Fuchs endothelial corneal dystrophy through the prism of oxidative stress","volume":"37","author":"Jurkunas","year":"2018","journal-title":"Cornea"},{"key":"10.1016\/j.omtn.2022.01.005_bib47","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1111\/j.1474-9726.2011.00723.x","article-title":"Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri","volume":"10","author":"Hartmann","year":"2011","journal-title":"Aging Cell"},{"key":"10.1016\/j.omtn.2022.01.005_bib48","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.omtn.2019.04.015","article-title":"Targeted transgene activation in the brain tissue by systemic delivery of engineered AAV1 expressing CRISPRa. Molecular therapy","volume":"16","author":"Lau","year":"2019","journal-title":"Nucleic Acids"},{"key":"10.1016\/j.omtn.2022.01.005_bib49","doi-asserted-by":"crossref","first-page":"109","DOI":"10.4161\/cbt.9.2.10583","article-title":"N-acetylcysteine, reactive oxygen species and beyond","volume":"9","author":"Sun","year":"2010","journal-title":"Cancer Biol. Ther."},{"key":"10.1016\/j.omtn.2022.01.005_bib50","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1093\/hmg\/ddr473","article-title":"An alpha 2 collagen VIII transgenic knock-in mouse model of Fuchs endothelial corneal dystrophy shows early endothelial cell unfolded protein response and apoptosis","volume":"21","author":"Jun","year":"2012","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.omtn.2022.01.005_bib51","doi-asserted-by":"crossref","first-page":"7245","DOI":"10.1073\/pnas.94.14.7245","article-title":"Distinct roles for E2F proteins in cell growth control and apoptosis","volume":"94","author":"DeGregori","year":"1997","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.omtn.2022.01.005_bib52","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1128\/MCB.16.3.1047","article-title":"Deregulated expression of E2F family members induces S-phase entry and overcomes p16INK4A-mediated growth suppression","volume":"16","author":"Lukas","year":"1996","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.omtn.2022.01.005_bib53","doi-asserted-by":"crossref","first-page":"705","DOI":"10.7150\/ijms.19018","article-title":"Characterization and prospective of human corneal endothelial progenitors","volume":"14","author":"Liu","year":"2017","journal-title":"Int. J. Med. Sci."},{"key":"10.1016\/j.omtn.2022.01.005_bib54","doi-asserted-by":"crossref","first-page":"3185","DOI":"10.1038\/emboj.2009.258","article-title":"E2F1 mediates DNA damage and apoptosis through HCF-1 and the MLL family of histone methyltransferases","volume":"28","author":"Tyagi","year":"2009","journal-title":"EMBO J."},{"key":"10.1016\/j.omtn.2022.01.005_bib55","doi-asserted-by":"crossref","first-page":"456","DOI":"10.4161\/rna.23547","article-title":"Paraspeckle formation during the biogenesis of long non-coding RNAs","volume":"10","author":"Naganuma","year":"2013","journal-title":"RNA Biol."},{"key":"10.1016\/j.omtn.2022.01.005_bib56","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/j.molcel.2009.01.026","article-title":"An architectural role for a nuclear noncoding RNA: NEAT1 RNA is essential for the structure of paraspeckles","volume":"33","author":"Clemson","year":"2009","journal-title":"Mol. Cell"},{"key":"10.1016\/j.omtn.2022.01.005_bib57","doi-asserted-by":"crossref","first-page":"11223","DOI":"10.1096\/fj.201900830R","article-title":"NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress","volume":"33","author":"Simchovitz","year":"2019","journal-title":"FASEB J."},{"key":"10.1016\/j.omtn.2022.01.005_bib58","doi-asserted-by":"crossref","first-page":"3129","DOI":"10.1080\/21691401.2019.1646264","article-title":"Long non-coding RNA NEAT1 inhibits oxidative stress-induced vascular endothelial cell injury by activating the miR-181d-5p\/CDKN3 axis","volume":"47","author":"Zhang","year":"2019","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"10.1016\/j.omtn.2022.01.005_bib59","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/02713680590927515","article-title":"Highly efficient ex\u00a0vivo gene delivery into human corneal endothelial cells by recombinant adeno-associated virus","volume":"30","author":"Lai","year":"2005","journal-title":"Curr. Eye Res."},{"key":"10.1016\/j.omtn.2022.01.005_bib60","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.brainresbull.2009.07.005","article-title":"Transduction efficiency of AAV 2\/6, 2\/8 and 2\/9 vectors for delivering genes in human corneal fibroblasts","volume":"81","author":"Sharma","year":"2010","journal-title":"Brain Res. Bull."},{"key":"10.1016\/j.omtn.2022.01.005_bib61","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1093\/hmg\/ddx028","article-title":"Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma","volume":"26","author":"O'Callaghan","year":"2017","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.omtn.2022.01.005_bib62","first-page":"2087","article-title":"Different tropism of adenoviruses and adeno-associated viruses to corneal cells: implications for corneal gene therapy","volume":"14","author":"Liu","year":"2008","journal-title":"Mol. Vis."},{"key":"10.1016\/j.omtn.2022.01.005_bib63","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1038\/nprot.2017.016","article-title":"Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening","volume":"12","author":"Joung","year":"2017","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.omtn.2022.01.005_bib64","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/nature14136","article-title":"Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex","volume":"517","author":"Konermann","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.omtn.2022.01.005_bib65","doi-asserted-by":"crossref","first-page":"107814","DOI":"10.1016\/j.exer.2019.107814","article-title":"Passaging capability of human corneal endothelial cells derived from old donors with and without accelerating cell attachment","volume":"189","author":"Parekh","year":"2019","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.omtn.2022.01.005_bib66","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1167\/iovs.06-1126","article-title":"A novel method of isolation, preservation, and expansion of human corneal endothelial cells","volume":"48","author":"Li","year":"2007","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.omtn.2022.01.005_bib67","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.cels.2019.03.003","article-title":"DoubletFinder: doublet detection in single-cell RNA sequencing data using artificial nearest neighbors","volume":"8","author":"McGinnis","year":"2019","journal-title":"Cell Syst."},{"key":"10.1016\/j.omtn.2022.01.005_bib68","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1038\/nmeth.4437","article-title":"Single-cell sequencing reveals dissociation-induced gene expression in tissue subpopulations","volume":"14","author":"van den Brink","year":"2017","journal-title":"Nat. Methods"},{"key":"10.1016\/j.omtn.2022.01.005_bib69","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1016\/j.cell.2019.05.031","article-title":"Comprehensive integration of single-cell data","volume":"177","author":"Stuart","year":"2019","journal-title":"Cell"},{"key":"10.1016\/j.omtn.2022.01.005_bib70","doi-asserted-by":"crossref","first-page":"W305","DOI":"10.1093\/nar\/gkp427","article-title":"ToppGene Suite for gene list enrichment analysis and candidate gene prioritization","volume":"37","author":"Chen","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.omtn.2022.01.005_bib71","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.1101\/gr.192237.115","article-title":"Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells","volume":"25","author":"Kowalczyk","year":"2015","journal-title":"Genome Res."},{"key":"10.1016\/j.omtn.2022.01.005_bib72","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/s41586-018-0698-6","article-title":"Single-cell reconstruction of the early maternal-fetal interface in humans","volume":"563","author":"Vento-Tormo","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.omtn.2022.01.005_bib73","doi-asserted-by":"crossref","first-page":"e0200005","DOI":"10.1371\/journal.pone.0200005","article-title":"Gene expression in the corneal endothelium of Fuchs endothelial corneal dystrophy patients with and without expansion of a trinucleotide repeat in TCF4","volume":"13","author":"Wieben","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.omtn.2022.01.005_bib74","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.omtn.2022.01.005_bib75","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1093\/bib\/bbs017","article-title":"Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration","volume":"14","author":"Thorvaldsdottir","year":"2013","journal-title":"Brief Bioinform."},{"key":"10.1016\/j.omtn.2022.01.005_bib76","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.omtn.2022.01.005_bib77","doi-asserted-by":"crossref","first-page":"e34309","DOI":"10.1371\/journal.pone.0034309","article-title":"Cochlin, intraocular pressure regulation and mechanosensing","volume":"7","author":"Goel","year":"2012","journal-title":"PLoS one"},{"key":"10.1016\/j.omtn.2022.01.005_bib78","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1038\/sj.gt.3301574","article-title":"In\u00a0vivo gene transfer to the mouse eye using an HIV-based lentiviral vector; efficient long-term transduction of corneal endothelium and retinal pigment epithelium","volume":"8","author":"Bainbridge","year":"2001","journal-title":"Gene Ther."}],"container-title":["Molecular Therapy Nucleic Acids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2162253122000105?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2162253122000105?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T05:58:03Z","timestamp":1750139883000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2162253122000105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3]]},"references-count":78,"alternative-id":["S2162253122000105"],"URL":"https:\/\/doi.org\/10.1016\/j.omtn.2022.01.005","relation":{},"ISSN":["2162-2531"],"issn-type":[{"value":"2162-2531","type":"print"}],"subject":[],"published":{"date-parts":[[2022,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Heterogeneity of human corneal endothelium implicates lncRNA NEAT1 in Fuchs endothelial corneal dystrophy","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy Nucleic Acids","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.omtn.2022.01.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors.","name":"copyright","label":"Copyright"}]}}