{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:04:47Z","timestamp":1790255087104,"version":"4.1.0"},"reference-count":31,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019470","name":"Lazio Innova","doi-asserted-by":"publisher","award":["CUP F89J23001120007"],"award-info":[{"award-number":["CUP F89J23001120007"]}],"id":[{"id":"10.13039\/100019470","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy Oncology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.omton.2026.201248","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T15:12:36Z","timestamp":1779981156000},"page":"201248","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intramuscular plasmid DNA electroporation sequesters neoantigen-specific CD8+ T cells in treated muscle and limits tumor infiltration"],"prefix":"10.1016","volume":"34","author":[{"given":"Andrea","family":"Bianchi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Esposito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiziano","family":"Giacomelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Esposito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Tonini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Aurisicchio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0473-4901","authenticated-orcid":false,"given":"Fabio","family":"Palombo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.omton.2026.201248_bib1","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.cell.2020.08.053","article-title":"Article A Phase Ib Trial of Personalized Neoantigen Therapy Plus Anti-PD-1 in Patients with Advanced Melanoma , Non-small Cell Lung Cancer , or Bladder Cancer","volume":"183","author":"Ott","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.omton.2026.201248_bib2","doi-asserted-by":"crossref","first-page":"LBA9512","DOI":"10.1200\/JCO.2024.42.17_suppl.LBA9512","article-title":"Individualized neoantigen therapy mRNA-4157 (V940) plus pembrolizumab in resected melanoma: 3-year update from the mRNA-4157-P201 (KEYNOTE-942) trial","volume":"42","author":"Weber","year":"2024","journal-title":"J. Clin. Oncol."},{"key":"10.1016\/j.omton.2026.201248_bib3","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.122857","article-title":"Combining STING-based neoantigen-targeted vaccine with checkpoint modulators enhances antitumor immunity in murine pancreatic cancer","volume":"3","author":"Kinkead","year":"2018","journal-title":"JCI Insight"},{"key":"10.1016\/j.omton.2026.201248_bib4","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/bs.adgen.2014.10.003","article-title":"Electroporation-mediated gene delivery","volume":"89","author":"Young","year":"2015","journal-title":"Adv. Genet."},{"key":"10.1016\/j.omton.2026.201248_bib5","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/s41467-024-55223-9","article-title":"Aptamer-conjugated gold nanoparticles enable oligonucleotide delivery into muscle stem cells to promote regeneration of dystrophic muscles","volume":"16","author":"Millozzi","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.omton.2026.201248_bib6","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1016\/S0140-6736(15)00239-1","article-title":"Safety , e ffi cacy , and immunogenicity of VGX-3100 , a therapeutic synthetic DNA vaccine targeting human papillomavirus 16 and 18 E6 and E7 proteins for cervical placebo-controlled phase 2b trial","volume":"386","author":"Trimble","year":"2015","journal-title":"Lancet"},{"key":"10.1016\/j.omton.2026.201248_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioelechem.2024.108802","article-title":"Long-term changes in transmembrane voltage after electroporation are governed by the interplay between nonselective leak current and ion channel activation","volume":"161","author":"Bla\u017ei\u010d","year":"2025","journal-title":"Bioelectrochemistry"},{"key":"10.1016\/j.omton.2026.201248_bib8","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1006\/mthe.2001.0483","article-title":"Electroporation-facilitated delivery of plasmid DNA in skeletal muscle: plasmid dependence of muscle damage and effect of poloxamer 188","volume":"4","author":"Hartikka","year":"2001","journal-title":"Mol. Ther."},{"key":"10.1016\/j.omton.2026.201248_bib9","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-68135-7","article-title":"Induction of a local muscular dystrophy using electroporation in vivo: an easy tool for screening therapeutics","volume":"10","author":"Derenne","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.omton.2026.201248_bib10","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1002\/jgm.416","article-title":"DNA transfection of mononuclear cells in muscle tissue","volume":"5","author":"Gr\u00f8nevik","year":"2003","journal-title":"J. Gene Med."},{"key":"10.1016\/j.omton.2026.201248_bib11","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1038\/gt.2011.198","article-title":"Molecular signature of the immune and tissue response to non-coding plasmid DNA in skeletal muscle after electrotransfer","volume":"19","author":"Mann","year":"2012","journal-title":"Gene Ther."},{"key":"10.1016\/j.omton.2026.201248_bib12","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1517\/14712598.8.11.1645","article-title":"Electroporation of skeletal muscle induces danger signal release and antigen-presenting cell recruitment independently of DNA vaccine administration","volume":"8","author":"Chiarella","year":"2008","journal-title":"Expert Opin. Biol. Ther."},{"key":"10.1016\/j.omton.2026.201248_bib13","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.3390\/vaccines10122037","article-title":"Transcriptomic Analysis of the Acute Skeletal Muscle Effects after Intramuscular DNA Electroporation Reveals Inflammatory Signaling","volume":"10","author":"Sales Conniff","year":"2022","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.omton.2026.201248_bib14","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/s41590-020-00810-3","article-title":"Intravenous nanoparticle vaccination generates stem-like TCF1+ neoantigen-specific CD8+ T cells","volume":"22","author":"Baharom","year":"2021","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.omton.2026.201248_bib15","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1172\/JCI93303","article-title":"Cancer vaccine formulation dictates synergy with CTLA-4 and PD-L1 checkpoint blockade therapy","volume":"128","author":"Hailemichael","year":"2018","journal-title":"J. Clin. Investig."},{"key":"10.1016\/j.omton.2026.201248_bib16","first-page":"1","article-title":"Poly-specific neoantigen-targeted cancer vaccines delay patient derived tumor growth","volume":"4","author":"Aurisicchio","year":"2019","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"10.1016\/j.omton.2026.201248_bib17","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1038\/s41541-022-00433-9","article-title":"Neoantigen cancer vaccine augments anti-CTLA-4 efficacy","volume":"7","author":"Salvatori","year":"2022","journal-title":"NPJ Vaccines"},{"key":"10.1016\/j.omton.2026.201248_bib18","doi-asserted-by":"crossref","first-page":"37","DOI":"10.4161\/onci.28540","article-title":"Dual therapeutic benefit of electroporation-mediated DNA vaccination in vivo: Enhanced gene transfer and adjuvant activity","volume":"3","author":"Calvet","year":"2014","journal-title":"Oncoimmunology"},{"key":"10.1016\/j.omton.2026.201248_bib19","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.ymthe.2021.09.011","article-title":"COVID-eVax, an electroporated DNA vaccine candidate encoding the SARS-CoV-2 RBD, elicits protective responses in animal models","volume":"30","author":"Conforti","year":"2022","journal-title":"Mol. Ther."},{"key":"10.1016\/j.omton.2026.201248_bib20","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/nm.3105","article-title":"Persistent antigen at vaccination sites induces tumor-specific CD8+ T cell sequestration, dysfunction and deletion","volume":"19","author":"Hailemichael","year":"2013","journal-title":"Nat. Med."},{"key":"10.1016\/j.omton.2026.201248_bib21","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1038\/s43018-022-00352-7","article-title":"Concurrent delivery of immune checkpoint blockade modulates T cell dynamics to enhance neoantigen vaccine-generated antitumor immunity","volume":"3","author":"Liu","year":"2022","journal-title":"Nat. Cancer"},{"key":"10.1016\/j.omton.2026.201248_bib22","doi-asserted-by":"crossref","first-page":"2408","DOI":"10.1172\/JCI128161","article-title":"Dysfunctional polycomb transcriptional repression contributes to lamin A\/C \u2013 dependent muscular dystrophy","volume":"130","author":"Bianchi","year":"2020","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.omton.2026.201248_bib23","doi-asserted-by":"crossref","DOI":"10.1080\/2162402X.2021.1898832","article-title":"Antitumor efficacy of a neoantigen cancer vaccine delivered by electroporation is influenced by microbiota composition","volume":"10","author":"Lione","year":"2021","journal-title":"Oncoimmunology"},{"key":"10.1016\/j.omton.2026.201248_bib26","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.omton.2026.201248_bib27","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.omton.2026.201248_bib28","article-title":"ComBat-seq: batch effect adjustment for RNA-seq count data","volume":"2","author":"Zhang","year":"2020","journal-title":"NAR Genom. Bioinf."},{"key":"10.1016\/j.omton.2026.201248_bib29","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1093\/bioinformatics\/bts034","article-title":"The sva package for removing batch effects and other unwanted variation in high-throughput experiments","volume":"28","author":"Leek","year":"2012","journal-title":"Bioinformatics"},{"key":"10.1016\/j.omton.2026.201248_bib30","article-title":"Fast gene set enrichment analysis","author":"Korotkevich","year":"2016","journal-title":"Preprint at bioRxiv"},{"key":"10.1016\/j.omton.2026.201248_bib31","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.3389\/fimmu.2018.01286","article-title":"seq-ImmuCC: cell-centric view of tissue transcriptome measuring cellular compositions of immune microenvironment from mouse RNA-Seq data","volume":"9","author":"Chen","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.omton.2026.201248_bib24","series-title":"FastQC: A Quality Control Tool for High Throughput Sequence Data","author":"Bittencourt","year":"2010"},{"key":"10.1016\/j.omton.2026.201248_bib25","doi-asserted-by":"crossref","first-page":"D493","DOI":"10.1093\/nar\/gkh103","article-title":"The UCSC Table Browser data retrieval tool","volume":"32","author":"Karolchik","year":"2004","journal-title":"Nucleic Acids Res."}],"updated-by":[{"DOI":"10.1016\/j.omton.2026.201313","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000}}],"container-title":["Molecular Therapy Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2950329926001244?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2950329926001244?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:34:36Z","timestamp":1790253276000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2950329926001244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["S2950329926001244"],"URL":"https:\/\/doi.org\/10.1016\/j.omton.2026.201248","relation":{},"ISSN":["2950-3299"],"issn-type":[{"value":"2950-3299","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Intramuscular plasmid DNA electroporation sequesters neoantigen-specific CD8+ T cells in treated muscle and limits tumor infiltration","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy Oncology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.omton.2026.201248","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc. on behalf of The American Society of Gene and Cell Therapy.","name":"copyright","label":"Copyright"}],"article-number":"201248"}}