{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T19:19:43Z","timestamp":1792351183732,"version":"4.4.0"},"reference-count":35,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","oooojournal.net","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology"],"published-print":{"date-parts":[[2012,12]]},"DOI":"10.1016\/j.oooo.2012.08.444","type":"journal-article","created":{"date-parts":[[2012,11,15]],"date-time":"2012-11-15T05:00:56Z","timestamp":1352955656000},"page":"764-770","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":34,"title":["Metagenomic investigation of microbes and viruses in patients with jaw osteonecrosis associated with bisphosphonate therapy"],"prefix":"10.1016","volume":"114","author":[{"given":"Parish P.","family":"Sedghizadeh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shibu","family":"Yooseph","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas W.","family":"Fadrosh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Zeigler-Allen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathangi","family":"Thiagarajan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Salek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farid","family":"Farahnik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon J.","family":"Williamson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.oooo.2012.08.444_bib1","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1345\/aph.1E357","article-title":"Discovery, clinical development, and therapeutic uses of bisphosphonates","volume":"39","author":"Licata","year":"2005","journal-title":"Ann Pharmacother"},{"key":"10.1016\/j.oooo.2012.08.444_bib2","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1016\/S0140-6736(10)62349-5","article-title":"Osteoporosis: now and the future","volume":"377","author":"Rachner","year":"2011","journal-title":"Lancet"},{"key":"10.1016\/j.oooo.2012.08.444_bib3","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.tripleo.2006.06.004","article-title":"Bisphosphonate-related osteonecrosis of the jaw: background and guidelines for diagnosis, staging and management","volume":"102","author":"Ruggiero","year":"2006","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"10.1016\/j.oooo.2012.08.444_bib4","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1053\/sarh.2002.33724b","article-title":"Pathogenesis and natural history of osteonecrosis","volume":"32","author":"Assouline-Dayan","year":"2002","journal-title":"Semin Arthritis Rheum"},{"key":"10.1016\/j.oooo.2012.08.444_bib5","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/nrendo.2010.195","article-title":"Osteonecrosis of the jaw and bisphosphonates in cancer: a narrative review","volume":"7","author":"Migliorati","year":"2011","journal-title":"Nat Rev Endocrinol"},{"key":"10.1016\/j.oooo.2012.08.444_bib6","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/j.joms.2007.11.035","article-title":"Identification of microbial biofilms in osteonecrosis of the jaws secondary to bisphosphonate therapy","volume":"66","author":"Sedghizadeh","year":"2008","journal-title":"J Oral Maxillofac Surg"},{"key":"10.1016\/j.oooo.2012.08.444_bib7","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.1600-0714.2006.00391.x","article-title":"Osteonecrosis of the jaws in patients treated with bisphosphonates\u2014histomorphologic analysis in comparison with infected osteoradionecrosis","volume":"35","author":"Hansen","year":"2006","journal-title":"J Oral Pathol Med"},{"key":"10.1016\/j.oooo.2012.08.444_bib8","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1007\/s00428-007-0516-2","article-title":"Actinomycosis of the jaws\u2014histopathological study of 45 patients shows significant involvement in bisphosphonate-associated osteonecrosis and infected osteoradionecrosis","volume":"451","author":"Hansen","year":"2007","journal-title":"Virchows Arch"},{"key":"10.1016\/j.oooo.2012.08.444_bib9","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.mehy.2011.04.015","article-title":"Bisphosphonates promote jaw osteonecrosis through facilitating bacterial colonisation","volume":"77","author":"Kos","year":"2011","journal-title":"Med Hypotheses"},{"key":"10.1016\/j.oooo.2012.08.444_bib10","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/s10856-005-0625-x","article-title":"Bacterial adhesion to bisphosphonate coated hydroxyapatite","volume":"16","author":"Ganguli","year":"2005","journal-title":"J Mater Sci Mater Med"},{"key":"10.1016\/j.oooo.2012.08.444_bib11","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.14219\/jada.archive.2009.0049","article-title":"Microbial biofilms in osteomyelitis of the jaw and osteonecrosis of the jaws secondary to bisphosphonate therapy","volume":"140","author":"Sedghizadeh","year":"2009","journal-title":"J Am Dent Assoc"},{"key":"10.1016\/j.oooo.2012.08.444_bib12","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1007\/s11914-010-0008-1","article-title":"The role of microbial biofilms in osteonecrosis of the jaw associated with bisphosphonate therapy","volume":"8","author":"Kumar","year":"2010","journal-title":"Curr Osteoporos Rep"},{"key":"10.1016\/j.oooo.2012.08.444_bib13","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.bone.2008.09.012","article-title":"Osteonecrosis of the jaw: who gets it, and why?","volume":"44","author":"Reid","year":"2009","journal-title":"Bone"},{"key":"10.1016\/j.oooo.2012.08.444_bib14","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1111\/j.1462-5822.2009.01323.x","article-title":"Evolving concepts in biofilm infections","volume":"11","author":"Hall-Stoodley","year":"2009","journal-title":"Cell Microbiol"},{"key":"10.1016\/j.oooo.2012.08.444_bib15","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1128\/CMR.15.2.167-193.2002","article-title":"Biofilms: survival mechanisms of clinically relevant microorganisms","volume":"15","author":"Donlan","year":"2002","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.oooo.2012.08.444_bib16","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1097\/01.blo.0000175714.68624.74","article-title":"Biofilms and antimicrobial resistance","volume":"437","author":"Patel","year":"2005","journal-title":"Clin Orthop Relat Res"},{"key":"10.1016\/j.oooo.2012.08.444_bib17","doi-asserted-by":"crossref","first-page":"613","DOI":"10.14219\/jada.archive.2003.0229","article-title":"How can oral health care providers determine if patients have dry mouth?","volume":"134","author":"Navazesh","year":"2003","journal-title":"J Am Dent Assoc"},{"key":"10.1016\/j.oooo.2012.08.444_bib18","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1177\/00220345870660S207","article-title":"Physiological factors affecting salivary flow rate, oral sugar clearance, and the sensation of dry mouth in man","volume":"66","author":"Dawes","year":"1987","journal-title":"J Dent Res"},{"key":"10.1016\/j.oooo.2012.08.444_bib19","doi-asserted-by":"crossref","first-page":"35S","DOI":"10.14219\/jada.archive.2008.0353","article-title":"Measuring salivary flow: challenges and opportunities","volume":"139","author":"Navazesh","year":"2008","journal-title":"J Am Dent Assoc"},{"key":"10.1016\/j.oooo.2012.08.444_bib20","doi-asserted-by":"crossref","first-page":"e17722","DOI":"10.1371\/journal.pone.0017722","article-title":"Single virus genomics: a new tool for virus discovery","volume":"23","author":"Allen","year":"2011","journal-title":"PLoS ONE"},{"key":"10.1016\/j.oooo.2012.08.444_bib21","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1126\/science.1093857","article-title":"Environmental genome shotgun sequencing of the Sargasso Sea","volume":"304","author":"Venter","year":"2004","journal-title":"Science"},{"key":"10.1016\/j.oooo.2012.08.444_bib22","doi-asserted-by":"crossref","first-page":"113","DOI":"10.3354\/ame014113","article-title":"Use of Sybr Green I for rapid epifluorescence counts of marine viruses and bacteria","volume":"14","author":"Noble","year":"1998","journal-title":"Aquat Microb Ecol"},{"key":"10.1016\/j.oooo.2012.08.444_bib23","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1101\/gr.112730.110","article-title":"Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons","volume":"21","author":"Haas","year":"2011","journal-title":"Genome Res"},{"key":"10.1016\/j.oooo.2012.08.444_bib24","doi-asserted-by":"crossref","first-page":"5261","DOI":"10.1128\/AEM.00062-07","article-title":"Naive bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy","volume":"73","author":"Wang","year":"2007","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.oooo.2012.08.444_bib25","doi-asserted-by":"crossref","first-page":"5002","DOI":"10.1128\/JB.00542-10","article-title":"The human oral microbiome","volume":"192","author":"Dewhirst","year":"2010","journal-title":"J Bacteriol"},{"key":"10.1016\/j.oooo.2012.08.444_bib26","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1186\/1471-2180-9-259","article-title":"Defining the healthy \u201ccore microbiome\u201d of oral microbial communities","volume":"9","author":"Zaura","year":"2009","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.oooo.2012.08.444_bib27","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1399-302X.2005.00254.x","article-title":"Characterization of Serratia marcescens isolates from subgingival biofilm, extraoral infections and environment by prodigiosin production, serotyping, and genotyping","volume":"21","author":"Barbosa","year":"2006","journal-title":"Oral Microbiol Immunol"},{"key":"10.1016\/j.oooo.2012.08.444_bib28","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1111\/j.1600-0757.2010.00361.x","article-title":"Bacterial and viral pathogens in saliva: disease relationship and infectious risk","volume":"55","author":"Slots","year":"2011","journal-title":"Periodontol 2000"},{"key":"10.1016\/j.oooo.2012.08.444_bib29","doi-asserted-by":"crossref","first-page":"2771","DOI":"10.1111\/j.1462-2920.2009.02101.x","article-title":"Roles of viruses in the environment","volume":"11","author":"Rohwer","year":"2009","journal-title":"Environ Microbiol"},{"key":"10.1016\/j.oooo.2012.08.444_bib30","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1111\/j.1601-0825.2012.01916.x","article-title":"Molecular profiling of oral microbiota in jawbone samples of bisphosphonate-related osteonecrosis of the jaw","volume":"18","author":"Wei","year":"2012","journal-title":"Oral Dis"},{"key":"10.1016\/j.oooo.2012.08.444_bib31","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1111\/j.1601-0825.2011.01848.x","article-title":"Antibiotic effects on bacterial profile in osteonecrosis of the jaw","volume":"18","author":"Ji","year":"2011","journal-title":"Oral Dis"},{"key":"10.1016\/j.oooo.2012.08.444_bib32","unstructured":"Saber MH, Alonaizan FA, Schwarzberg K, Kelley ST, Sedghizadeh PP, Levy TA, et al. Bacterial flora of dental periradicular lesions analyzed by the 454-pyrosequencing technology. J Endod. In press."},{"key":"10.1016\/j.oooo.2012.08.444_bib33","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1101\/gr.085464.108","article-title":"Microbial community profiling for human microbiome projects: tools, techniques, and challenges","volume":"19","author":"Hamady","year":"2009","journal-title":"Genome Res"},{"key":"10.1016\/j.oooo.2012.08.444_bib34","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1016\/j.joen.2007.09.002","article-title":"Bacterial DNA persists for extended periods after cell death","volume":"33","author":"Young","year":"2007","journal-title":"J Endod"},{"key":"10.1016\/j.oooo.2012.08.444_bib35","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.joms.2009.01.011","article-title":"Bisphosphonates-what the dentist needs to know: practical considerations","volume":"67","author":"Fantasia","year":"2009","journal-title":"J Oral Maxillofac Surg"}],"container-title":["Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2212440312014976?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2212440312014976?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T21:58:02Z","timestamp":1761947882000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2212440312014976"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["S2212440312014976"],"URL":"https:\/\/doi.org\/10.1016\/j.oooo.2012.08.444","relation":{},"ISSN":["2212-4403"],"issn-type":[{"value":"2212-4403","type":"print"}],"subject":[],"published":{"date-parts":[[2012,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Metagenomic investigation of microbes and viruses in patients with jaw osteonecrosis associated with bisphosphonate therapy","name":"articletitle","label":"Article Title"},{"value":"Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.oooo.2012.08.444","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2012 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}