{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T19:16:36Z","timestamp":1780427796152,"version":"3.54.1"},"reference-count":36,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T00:00:00Z","timestamp":1730937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007214","name":"Velux Foundation","doi-asserted-by":"publisher","award":["1465"],"award-info":[{"award-number":["1465"]}],"id":[{"id":"10.13039\/100007214","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ophthalmology Retina"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1016\/j.oret.2024.11.003","type":"journal-article","created":{"date-parts":[[2024,11,9]],"date-time":"2024-11-09T01:04:29Z","timestamp":1731114269000},"page":"444-452","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["Clinical Evaluation of a Novel CRISPR-Cas12a-Based RID-MyC Assay for the Diagnosis of Fungal Endophthalmitis"],"prefix":"10.1016","volume":"9","author":[{"given":"Hanith Raj","family":"Deivarajan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prabhakara Sethupathy","family":"R","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vignesh","family":"Elamurugan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akshayaa","family":"VS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reega","family":"P","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dharani","family":"Chelliah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hari Vignesh","family":"S","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elakkiya","family":"Nandhini GR","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanmani","family":"M","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dharsini","family":"Nandhakumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karvannan","family":"Sevugamurthi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saravanan","family":"VR","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuradha","family":"K","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parag K.","family":"Shah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram","family":"Rammohan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishek","family":"Nair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krutin","family":"Shah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anju","family":"Jose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaishree","family":"Pandian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lalitha","family":"Prajna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkatesh N.","family":"Prajna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9220-8049","authenticated-orcid":false,"given":"Siddharth","family":"Narendran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.oret.2024.11.003_bib1","first-page":"57","article-title":"Global and multi-national prevalence of fungal diseases-estimate precision","volume":"3","author":"Bongomin","year":"2017","journal-title":"J\u00a0Fungi (Basel)"},{"key":"10.1016\/j.oret.2024.11.003_bib2","first-page":"5075","article-title":"Misdiagnosis of invasive pulmonary aspergillosis: a clinical analysis of 26 immunocompetent patients","volume":"7","author":"Zhang","year":"2014","journal-title":"Int J Clin Exp Med"},{"key":"10.1016\/j.oret.2024.11.003_bib3","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-4939-6515-1_1","article-title":"Current challenges in the diagnosis of fungal infections","volume":"1508","author":"Lass-Fl\u00f6rl","year":"2017","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.oret.2024.11.003_bib4","doi-asserted-by":"crossref","first-page":"2643","DOI":"10.1128\/jcm.33.10.2643-2646.1995","article-title":"Inhibition of PCR by aqueous and vitreous fluids","volume":"33","author":"Wiedbrauk","year":"1995","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.oret.2024.11.003_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.idc.2015.10.004","article-title":"The global burden of fungal diseases","volume":"30","author":"Vallabhaneni","year":"2016","journal-title":"Infect Dis Clin North Am"},{"key":"10.1016\/j.oret.2024.11.003_bib6","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/S1473-3099(22)00656-9","article-title":"Diagnostic capacity for invasive fungal infections in advanced HIV disease in Africa: a continent-wide survey","volume":"23","author":"Lakoh","year":"2023","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.oret.2024.11.003_bib7","first-page":"996","article-title":"Fungal endophthalmitis: a comprehensive review","volume":"7","author":"Haseeb","year":"2021","journal-title":"J\u00a0Fungi (Basel)"},{"key":"10.1016\/j.oret.2024.11.003_bib8","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.oret.2021.09.006","article-title":"Fungal endophthalmitis: analysis of 730 consecutive eyes from 7 tertiary eye care centers in India","volume":"6","author":"Das","year":"2022","journal-title":"Ophthalmol Retina"},{"key":"10.1016\/j.oret.2024.11.003_bib9","doi-asserted-by":"crossref","first-page":"411","DOI":"10.3390\/microorganisms7100411","article-title":"Targeted high-throughput sequencing identifies predominantly fungal pathogens in patients with clinically infectious, culture-negative endophthalmitis in South India","volume":"7","author":"Gandhi","year":"2019","journal-title":"Microorganisms"},{"key":"10.1016\/j.oret.2024.11.003_bib10","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.oret.2024.11.003_bib11","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1038\/s41551-021-00760-7","article-title":"CRISPR-based diagnostics","volume":"5","author":"Kaminski","year":"2021","journal-title":"Nat Biomed Eng"},{"key":"10.1016\/j.oret.2024.11.003_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.xops.2024.100522","article-title":"Development and clinical evaluation of a CRISPR\/Cas12a-based nucleic acid detection platform for the diagnosis of keratomycoses","volume":"4","author":"Deivarajan","year":"2024","journal-title":"Ophthalmol Sci"},{"key":"10.1016\/j.oret.2024.11.003_bib13","doi-asserted-by":"crossref","first-page":"679","DOI":"10.3390\/diagnostics12030679","article-title":"The diagnosis and treatment of fungal endophthalmitis: an update","volume":"12","author":"Danielescu","year":"2022","journal-title":"Diagnostics (Basel)"},{"key":"10.1016\/j.oret.2024.11.003_bib14","doi-asserted-by":"crossref","first-page":"2913","DOI":"10.1128\/jcm.33.11.2913-2919.1995","article-title":"Molecular probes for diagnosis of fungal infections","volume":"33","author":"Sandhu","year":"1995","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.oret.2024.11.003_bib15","first-page":"YE01","article-title":"Requirements for minimum sample size for sensitivity and specificity analysis","volume":"10","author":"Bujang","year":"2016","journal-title":"J\u00a0Clin Diagn Res"},{"key":"10.1016\/j.oret.2024.11.003_bib16","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.2147\/OPTH.S67589","article-title":"Bacterial endogenous endophthalmitis in Vietnam: a randomized controlled trial comparing vitrectomy with silicone oil versus vitrectomy alone","volume":"8","author":"Do","year":"2014","journal-title":"Clin Ophthalmol"},{"key":"10.1016\/j.oret.2024.11.003_bib17","doi-asserted-by":"crossref","first-page":"177","DOI":"10.3201\/eid2302.152042","article-title":"Delivering on antimicrobial resistance agenda not possible without improving fungal diagnostic capabilities","volume":"23","author":"Denning","year":"2017","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.oret.2024.11.003_bib18","author":"Mosbergen"},{"key":"10.1016\/j.oret.2024.11.003_bib19","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.jcjo.2022.04.007","article-title":"Misdiagnosis of fungal infections of the orbit","volume":"58","author":"Simmons","year":"2023","journal-title":"Can J Ophthalmol"},{"key":"10.1016\/j.oret.2024.11.003_bib20","first-page":"460","article-title":"Pulmonary and extrapulmonary manifestations of fungal infections misdiagnosed as tuberculosis: the need for prompt diagnosis and management","volume":"8","author":"Ekeng","year":"2022","journal-title":"J\u00a0Fungi (Basel)"},{"issue":"suppl 2","key":"10.1016\/j.oret.2024.11.003_bib21","doi-asserted-by":"crossref","first-page":"S22","DOI":"10.1038\/leusup.2012.14","article-title":"Fungal infections: diagnostic problems and choice of therapy","volume":"1","author":"de Pauw","year":"2012","journal-title":"Leuk Suppl"},{"key":"10.1016\/j.oret.2024.11.003_bib22","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1111\/myc.12822","article-title":"Fungal keratitis: an overview of clinical and laboratory aspects","volume":"61","author":"Mahmoudi","year":"2018","journal-title":"Mycoses"},{"key":"10.1016\/j.oret.2024.11.003_bib23","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1167\/iovs.11-8478","article-title":"The clinical differentiation of bacterial and fungal keratitis: a photographic survey","volume":"53","author":"Dalmon","year":"2012","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.oret.2024.11.003_bib24","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1080\/17469899.2020.1820322","article-title":"Diagnosis and management of fungal endophthalmitis: India perspective","volume":"15","author":"Das","year":"2020","journal-title":"Expert Rev Ophthalmol"},{"key":"10.1016\/j.oret.2024.11.003_bib25","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1038\/s41433-020-01339-3","article-title":"Infectious keratitis: an update on epidemiology, causative microorganisms, risk factors, and antimicrobial resistance","volume":"35","author":"Ting","year":"2021","journal-title":"Eye (Lond)"},{"key":"10.1016\/j.oret.2024.11.003_bib26","doi-asserted-by":"crossref","first-page":"912","DOI":"10.4103\/ijo.IJO_821_17","article-title":"Fungal keratitis: the Aravind experience","volume":"65","author":"Prajna","year":"2017","journal-title":"Indian J Ophthalmol"},{"key":"10.1016\/j.oret.2024.11.003_bib27","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/S0161-6420(00)00517-0","article-title":"Use of polymerase chain reaction in the diagnosis of fungal endophthalmitis","volume":"108","author":"Anand","year":"2001","journal-title":"Ophthalmology"},{"key":"10.1016\/j.oret.2024.11.003_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2021.111772","article-title":"CRISPR is a useful biological tool for detecting nucleic acid of SARS-CoV-2 in human clinical samples","volume":"140","author":"Rahman","year":"2021","journal-title":"Biomed Pharmacother"},{"issue":"7","key":"10.1016\/j.oret.2024.11.003_bib29","doi-asserted-by":"crossref","first-page":"e48","DOI":"10.1016\/j.ophtha.2013.02.026","article-title":"Keratitis-Induced Endophthalmitis","volume":"120","author":"Chen","year":"2013","journal-title":"Ophthalmology"},{"key":"10.1016\/j.oret.2024.11.003_bib30","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1099\/00222615-46-3-188","article-title":"The possibilities and limitations of nucleic acid amplification technology in diagnostic\u00a0microbiology","volume":"46","author":"Vaneechoutte","year":"1997","journal-title":"J\u00a0Med Microbiol"},{"key":"10.1016\/j.oret.2024.11.003_bib31","doi-asserted-by":"crossref","first-page":"768","DOI":"10.4103\/ijo.IJO_2329_21","article-title":"Understanding the science of fungal endophthalmitis - AIOS 2021 Sengamedu Srinivas Badrinath Endowment Lecture","volume":"70","author":"Das","year":"2022","journal-title":"Indian J Ophthalmol"},{"key":"10.1016\/j.oret.2024.11.003_bib32","series-title":"Fungal endophthalmitis. Treasure Island, FL","author":"Ly","year":"2024"},{"key":"10.1016\/j.oret.2024.11.003_bib33","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1016\/j.ophtha.2013.07.002","article-title":"Toward first-line molecular diagnosis of ocular infectious disease","volume":"120","author":"Van Gelder","year":"2013","journal-title":"Ophthalmology"},{"key":"10.1016\/j.oret.2024.11.003_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2021.e06780","article-title":"Targeted metagenomics using next generation sequencing in laboratory diagnosis of culture negative endophthalmitis","volume":"7","author":"Mishra","year":"2021","journal-title":"Heliyon"},{"key":"10.1016\/j.oret.2024.11.003_bib35","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1038\/s41591-022-01734-1","article-title":"Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants","volume":"28","author":"Welch","year":"2022","journal-title":"Nat Med"},{"key":"10.1016\/j.oret.2024.11.003_bib36","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1471-2415-8-7","article-title":"Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis","volume":"8","author":"Embong","year":"2008","journal-title":"BMC Ophthalmol"}],"container-title":["Ophthalmology Retina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2468653024005335?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2468653024005335?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T19:07:05Z","timestamp":1762628825000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2468653024005335"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":36,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["S2468653024005335"],"URL":"https:\/\/doi.org\/10.1016\/j.oret.2024.11.003","relation":{},"ISSN":["2468-6530"],"issn-type":[{"value":"2468-6530","type":"print"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Clinical Evaluation of a Novel CRISPR-Cas12a-Based RID-MyC Assay for the Diagnosis of Fungal Endophthalmitis","name":"articletitle","label":"Article Title"},{"value":"Ophthalmology Retina","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.oret.2024.11.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"name":"copyright","label":"Copyright"}]}}