{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T03:02:17Z","timestamp":1778295737957,"version":"3.51.4"},"reference-count":8,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Forty years ago, Marshall Urist described a partially purified extract of demineralized bone matrix which induced the formation of ectopic bone. This substance, bone morphogenetic protein\/non\u2010collagenous protein (BMP\/NCP), was never purified to homogeneity but other investigators used similar starting materials to clone a number of recombinant BMPs. Urist recognized that his material probably contained the BMPs which had been cloned by others but always contended that it contained another, more potent, bone inducing material which differed significantly in its physical and chemical properties from the known BMPs. We have used Urist's protocol to isolate a protein that has the chemical and physical properties of Urist's \u201cBMP\u201d. It is an 18.5 kD fragment of the bone matrix protein, SPP\u201024. This fragment contains the cystatin\u2010like domain of SPP\u201024. We have located a 19 amino acid region which is similar to the TGF\u2010\u03b2\/BMP\u2010binding region of fetuin, a member of the cystatin family of protease inhibitors. A cyclic peptide, which we call BMP binding peptide (BBP) was generated using this sequence. The peptide avidly bound rhBMP\u20102 with a <jats:italic>K<\/jats:italic><jats:sub>D<\/jats:sub> of 3 \u00d7 10<jats:sup>\u22125<\/jats:sup> M. When implanted alone in mouse muscle, the peptide frequently induced dystrophic calcification. When implanted with rhBMP\u20102, the peptide enhanced the osteogenic activity of the recombinant molecule. We hypothesize that Urist's \u201cBMP\u201d was a fragment of SPP\u201024 which influenced bone induction by binding to bone morphogenetic proteins. BBP may be clinically useful because of its effects on other bone\u2010inducing substances. Published by Elsevier Ltd. on behalf of Orthopaedic Research Society. \u00a9 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.<\/jats:p>","DOI":"10.1016\/j.orthres.2004.05.001","type":"journal-article","created":{"date-parts":[[2004,8,5]],"date-time":"2004-08-05T04:27:27Z","timestamp":1091680047000},"page":"175-180","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["BMP binding peptide: A BMP\u20102 enhancing factor deduced from the sequence of native bovine bone morphogenetic protein\/non\u2010collagenous protein"],"prefix":"10.1002","volume":"23","author":[{"given":"Keyvan","family":"Behnam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin L.","family":"Phillips","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose Denison Prado","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elsa J.","family":"Brochmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Eugenia Leite","family":"Duarte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel S.","family":"Murray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560060102"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.22.12755"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.1.431"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4042"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.150.3698.893"},{"key":"e_1_2_1_7_2","volume-title":"Methods in enzymology","author":"Urist MR","year":"1987"},{"key":"e_1_2_1_8_2","volume-title":"Development and diseases of cartilage and bone matrix","author":"Urist MR","year":"1987"},{"key":"e_1_2_1_9_2","volume-title":"Fundamentals of bone growth: methodology and applications","author":"Urist MR","year":"1991"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.orthres.2004.05.001","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.orthres.2004.05.001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T19:15:59Z","timestamp":1697138159000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1016\/j.orthres.2004.05.001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1]]},"references-count":8,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,1]]}},"alternative-id":["10.1016\/j.orthres.2004.05.001"],"URL":"https:\/\/doi.org\/10.1016\/j.orthres.2004.05.001","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,1]]},"assertion":[{"value":"2006-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}