{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:28:54Z","timestamp":1761006534830,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T00:00:00Z","timestamp":1463443200000},"content-version":"vor","delay-in-days":4215,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:sec><jats:title>OBJECTIVES<\/jats:title><jats:p>To investigate the possible role of eosinophil peroxidase (EPO) in 3\u2010nitrotyrosine (3NT) formation.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND SETTING<\/jats:title><jats:p>Observational study employing immunocytochemistry to assess the presence of 3NT, inducible nitric oxide synthase (iNOS), eosinophils, mast cells, neutrophils, and lymphocytes in ethmoid sinus mucosal biopsies from normal controls and subjects with allergic and nonallergic chronic sinusitis and nasal polyposis.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>3NT was more evident in biopsies from sinusitis patients (2.67 \u00b1 0.14, n = 21) than in healthy mucosa (0.43 \u00b1 0.2, n = 7, <jats:italic>P<\/jats:italic> &lt; 0.01), but scores in atopic and nonatopic subjects were similar. Colocalization studies confirmed that 3NT was largely confined to eosinophils. No relationship was found between 3NT and other immune cells. 3NT detection was not correlated with the amount of immunostaining for iNOS.<\/jats:p><\/jats:sec><jats:sec><jats:title>SIGNIFICANCE<\/jats:title><jats:p>Chronic sinusitis is accompanied by 3NT formation, which is largely restricted to the eosinophils, and likely driven by the action of eosinophil peroxidase, rather than by nitric oxide levels. EBM rating: B\u20102.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.otohns.2004.05.013","type":"journal-article","created":{"date-parts":[[2004,10,30]],"date-time":"2004-10-30T14:25:44Z","timestamp":1099146344000},"page":"696-703","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Protein Nitration in Chronic Sinusitis and Nasal Polyposis: Role of Eosinophils"],"prefix":"10.1002","volume":"131","author":[{"given":"Julio Freire","family":"Bernardes","sequence":"first","affiliation":[{"name":"Meakins\u2010Christie Laboratories, Respiratory Division Department of Medicine McGill University Health Centre Montreal Quebec Canada"},{"name":"Department of Otolaryngology SMDB\u2010Jewish General Hospital Montreal Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jichuan","family":"Shan","sequence":"additional","affiliation":[{"name":"Meakins\u2010Christie Laboratories, Respiratory Division Department of Medicine McGill University Health Centre Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Tewfik","sequence":"additional","affiliation":[{"name":"Meakins\u2010Christie Laboratories, Respiratory Division Department of Medicine McGill University Health Centre Montreal Quebec Canada"},{"name":"Department of Otolaryngology SMDB\u2010Jewish General Hospital Montreal Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qutayba","family":"Hamid","sequence":"additional","affiliation":[{"name":"Meakins\u2010Christie Laboratories, Respiratory Division Department of Medicine McGill University Health Centre Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saul","family":"Frenkiel","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology SMDB\u2010Jewish General Hospital Montreal Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David H.","family":"Eidelman","sequence":"additional","affiliation":[{"name":"Meakins\u2010Christie Laboratories, Respiratory Division Department of Medicine McGill University Health Centre Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,17]]},"reference":[{"issue":"6","key":"e_1_2_5_2_1","first-page":"S1","article-title":"Sinusitis: Bench to bedside. 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