{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:03:55Z","timestamp":1791569035103,"version":"4.3.4"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T00:00:00Z","timestamp":1463443200000},"content-version":"vor","delay-in-days":3638,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:sec><jats:title>OBJECTIVES<\/jats:title><jats:p>To determine whether biofilm\u2010forming capacity of bacteria demonstrated in chronic rhinosinusitis (CRS) has an impact on persistence of the disease following endoscopic sinus surgery (ESS).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHOD<\/jats:title><jats:p>Thirty\u2010one bacterial strains recovered from 19 patients with CRS at least 1 year post\u2010ESS. Evolution of disease was assessed by questionnaire and endoscopy as favorable or unfavorable. The bacteria were cultured on a 96\u2010well culture plaque and a semi\u2010quantitative method using crystal violet to quantify biofilm production was used.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Twenty\u2010two of 31 samples produced a biofilm thicker or equal to the positive control. Biofilm production was noted in 6\/10 <jats:italic>Pseudomonas aeruginosa<\/jats:italic> isolates, 8\/10 <jats:italic>Staphylococcus aureus<\/jats:italic>, and 8\/11 coagulase\u2010negative staphylococci. Biofilm formation was associated with a poor evolution for <jats:italic>Pseudomonas aeruginosa<\/jats:italic> and Staphylococcus aureus, but not coagulase\u2010negative staphylococcus.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>There is a correlation between in vitro biofilm\u2010producing capacity by <jats:italic>Pseudomonas aeruginosa<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic> and unfavorable evolution after ESS, suggesting a role for biofilm production in chronic sinusitis.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.otohns.2006.03.001","type":"journal-article","created":{"date-parts":[[2006,5,26]],"date-time":"2006-05-26T07:27:42Z","timestamp":1148628462000},"page":"991-996","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":245,"title":["Biofilm Formation by <i>Staphylococcus Aureus and Pseudomonas Aeruginosa<\/i> is Associated with an Unfavorable Evolution after Surgery 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