{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T10:14:31Z","timestamp":1790331271881,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T00:00:00Z","timestamp":1463443200000},"content-version":"vor","delay-in-days":3365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate if analysis of pepsin\/pepsinogen in middle ear effusions can be considered a diagnostic marker for laryngopharyngeal reflux (LPR) in children with otitis media with effusion (OME).<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Ambulatory 24\u2010hour dualprobe pH monitoring was carried out on 31 children with OME. Middle ear effusions were collected from 17 children during myringotomy. Total pepsin\/pepsinogen concentrations in effusions were measured by ELISA using antipepsin antibody.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Dual\u2010probe pH monitoring showed that 22\/31 (71%) of the studied children had significant LPR. The concentrations of pepsin\/pepsinogen in middle ear effusions, ranged from 0.085 to 5.02 \u03bcg\/ml, were found to be up to 4.5 to 231.44 times higher than the serum levels. There was a significant positive correlation between the level of pepsin\/pepsinogen assayed in the effusions of the 17 children and the number of pharyngeal reflux episodes measured by pH monitoring.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Control of LPR may be an essential component in the successful management of OME in pediatric patients. Pepsin\/pepsinogen analysis in effusions of children, using ELISA, can be considered a reliable marker for assessment of reflux in children with OME.<\/jats:p><jats:p>\u00a9 2007 American Academy of Otolaryngology\u2010Head and Neck Surgery Foundation. All rights reserved.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.otohns.2006.08.030","type":"journal-article","created":{"date-parts":[[2007,2,26]],"date-time":"2007-02-26T10:43:42Z","timestamp":1172486622000},"page":"464-470","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Pepsin assay: A marker for reflux in pediatric glue ear"],"prefix":"10.1002","volume":"136","author":[{"given":"Ahmed M.","family":"Abd El\u2010Fattah","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Faculty of Medicine\u2010Mansoura University Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gamal A.","family":"Abdul Maksoud","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Faculty of Medicine\u2010Zagazig University Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed S.","family":"Ramadan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Faculty of Medicine\u2010Zagazig University Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed F.","family":"Abdalla","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Faculty of Medicine\u2010Mansoura University Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed M.","family":"Abdel Aziz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Gastroenterology Center Faculty of Medicine\u2010Mansoura University Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2004.07.019"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940111001007"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200206000-00004"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200211000-00004"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2005.05.654"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003489400109S1003"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1536-4801.2001.tb07189.x"},{"key":"e_1_2_6_9_1","first-page":"14","article-title":"Laryngopharyngeal reflux testing.","volume":"81","author":"Postma GN","year":"2002","journal-title":"Ear Nose Throat J"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/000992289303200806"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/archpedi.154.2.150"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005176-199205000-00017"},{"key":"e_1_2_6_13_1","first-page":"25","article-title":"Normal and abnormal proximal esophageal acid exposure: results of ambulatory dual\u2010probe pH monitoring","volume":"88","author":"Dubhan R","year":"1993","journal-title":"Am J Gastroenterol"},{"key":"e_1_2_6_14_1","unstructured":"PanettiM RevilleJ ClyneS et alClinical implications of the functional stability of pepsin. 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