{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:45:55Z","timestamp":1761007555235,"version":"build-2065373602"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T00:00:00Z","timestamp":1463443200000},"content-version":"vor","delay-in-days":3243,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:sec><jats:title>OBJECTIVE<\/jats:title><jats:p>To determine if furosemide could have a role in inhibition of biofilm formation.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN<\/jats:title><jats:p>A prospective study of 72\u2010hour biofilms grown in the Calgary Biofilm Device (CBD) treated with furosemide and controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Bacteria were treated with furosemide and controls to determine its antiplanktonic properties. PA01 biofilms were treated with serial 2\u2010fold dilutions of furosemide and controls over a 48\u2010hour period at pH concentrations of 7.0, 8.0, and 9.0.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Furosemide had no effect on planktonic PA01. It had minimal effect at pH below 8.0. A pH of 8.0 and 9.0 reduced biofilms 25%. Furosemide 10\u00a0mg\/mL reduced biofilms 50% at pH 8.0 and 9.0. It inhibited biofilms to &lt;30% at concentrations of 0.6 to 0.16\u00a0mg\/mL at pH 8.0 and &lt;10% at concentrations of 1.25 to 0.3\u00a0mg\/mL at pH 9.0.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Furosemide was found to be nonantiplanktonic, but does appear to destabilize preformed PA01 biofilms at pH\u2010dependent concentrations.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.otohns.2007.02.022","type":"journal-article","created":{"date-parts":[[2007,6,26]],"date-time":"2007-06-26T07:18:32Z","timestamp":1182842312000},"page":"21-26","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The Impact of a Cation Channel Blocker (Furosemide) on <i>Pseudomonas aeruginosa<\/i> PA01 Biofilm Architecture"],"prefix":"10.1002","volume":"137","author":[{"given":"Jenny L.","family":"Cross","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Head\u2010Neck Surgery West Virginia University Morgantown WV"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hassan H.","family":"Ramadan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head\u2010Neck Surgery West Virginia University Morgantown WV"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John G.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head\u2010Neck Surgery West Virginia University Morgantown WV"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,17]]},"reference":[{"key":"e_1_2_20_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5409.1837"},{"key":"e_1_2_20_3_1","unstructured":"Edstrom Industries Inc.Steps in Biofilm Development 2004.Available at:http:\/\/www.edstrom.com\/Resources.cfm?doc_id=143. 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