{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T19:53:43Z","timestamp":1774295623743,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:sec><jats:title>OBJECTIVE<\/jats:title><jats:p>To assess immune\u2010based gene therapy in a murine floor of mouth (FOM) squamous cell carcinoma (SCC) model.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN<\/jats:title><jats:p>In vitro and in vivo testing of immune therapy for SCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Multiple SCC lines were infected by using advRSV\u2010interleukin\u201012 (IL\u201012) and advCMV\u2010interleukin\u201012\/granulocyte macrophage colony\u2010stimulating factor (IL\u201012\/GM\u2010CSF) and monitored for production of IL\u201012 and GM\u2010CSF. Intratumoral injections of viral vectors were administered with systemic Ig\u20104\u20101BB ligand in an orthotopic murine FOM SCC model and followed for tumor size and survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In vitro, all cell lines produced substantial levels of IL\u201012 and GM\u2010CSF. In vivo, tumors treated with advCMV\u2010IL\u201012\/GM\u2010CSF and Ig\u20104\u20101BBL showed a striking reduction in tumor volume (vs control <jats:italic>P<\/jats:italic> &lt; 0.0001) and improved median survival (38\u00a0days vs 19\u00a0days for control, <jats:italic>P<\/jats:italic> &lt; 0.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Combination immune\u2010based therapies effectively improve survival in mice bearing FOM SCC over single\u2010modality therapy.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.otohns.2008.05.001","type":"journal-article","created":{"date-parts":[[2008,8,23]],"date-time":"2008-08-23T07:18:53Z","timestamp":1219475933000},"page":"442-448","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The administration of IL\u201012\/GM\u2010CSF and Ig\u20104\u20101BB ligand markedly decreases murine floor of mouth squamous cell cancer"],"prefix":"10.1002","volume":"139","author":[{"given":"Nithin D.","family":"Adappa","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Mount Sinai School of Medicine New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi\u2010Kwang","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Mount Sinai School of Medicine New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Mount Sinai School of Medicine New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian\u2010Gui","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Gene and Cell Medicine Mount Sinai School of Medicine New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric M.","family":"Genden","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Mount Sinai School of Medicine New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward J.","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Mount Sinai School of Medicine New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1992.10.2.257"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-005-5050-6"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.2001.0402"},{"key":"e_1_2_8_5_1","first-page":"2488","article-title":"A genetic progression model for head and neck cancer: implications for field cancerization","volume":"56","author":"Califano J","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.11.6.667"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199903000-00009"},{"key":"e_1_2_8_8_1","first-page":"1737","article-title":"Combination gene therapy for oral cancer in a murine model","volume":"56","author":"O'Malley BW","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990531)81:5<813::AID-IJC24>3.0.CO;2-I"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11696"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302556"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.8.4779"},{"key":"e_1_2_8_13_1","unstructured":"ChenL HuangTG MeseckML et al.Rejection of metastic 4T1 breast cancer by attenuating Treg cells in combination with immune stimulation. 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