{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T07:30:53Z","timestamp":1769499053110,"version":"3.49.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To identify <jats:italic>Helicobacter pylori<\/jats:italic> and major virulance factor, <jats:italic>cag<\/jats:italic>A, in patients with laryngeal diseases and nasal polyps.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Cross\u2010sectional study with planned data collection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>The study was performed on fresh tissue samples from patients with 32\u00a0nasal polyps, 29\u00a0normal nasal mucosa, and 27 laryngeal diseases presenting to the Otolaryngology\u2013Head and Neck Surgery department of a major military hospital in Istanbul, Turkey.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Tissue specimens were evaluated by in\u2010house polymerase chain reaction (PCR) and real\u2010time PCR for bacterial DNA and by real\u2010time PCR for <jats:italic>cag<\/jats:italic>A. The impact of commercial and in\u2010house DNA extraction methods was also evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>H pylori<\/jats:italic> DNA was detected only by real\u2010time PCR in 59.4 percent of nasal polyps, 70.4 percent of nasal mucosa samples, and 58.6 percent of larynx samples. <jats:italic>cag<\/jats:italic>A was identified in 78.9, 89.5, and 82.4 percent of positive polyp, nasal mucosa, and larynx samples, respectively. No statistically significant differences were observed between groups. DNA purification methods were equally effective.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>H pylori<\/jats:italic> DNA is present in nasal polyp and larynx tissues as well as normal nasal mucosa, as detected by a sensitive real\u2010time PCR assay. <jats:italic>cag<\/jats:italic>A\u2010positive strains dominate in all groups.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.otohns.2009.04.005","type":"journal-article","created":{"date-parts":[[2009,6,26]],"date-time":"2009-06-26T09:44:15Z","timestamp":1246009455000},"page":"131-135","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Real\u2010time PCR detection of <i>Helicobacter pylori<\/i> and virulence\u2010associated <i>cag<\/i>A in nasal polyps and laryngeal disorders"],"prefix":"10.1002","volume":"141","author":[{"given":"Mustafa","family":"Ozyurt","sequence":"first","affiliation":[{"name":"Department of Microbiology and Clinical Microbiology Gulhane Military Medical Academy Haydarpasa Training Hospital Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atila","family":"Gungor","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Gulhane Military Medical Academy Haydarpasa Training Hospital Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koray","family":"Ergunay","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Clinical Microbiology Faculty of Medicine Hacettepe University Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Engin","family":"Cekin","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Gulhane Military Medical Academy Haydarpasa Training Hospital Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evren","family":"Erkul","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Gulhane Military Medical Academy Haydarpasa Training Hospital Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuncer","family":"Haznedaroglu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Clinical Microbiology Gulhane Military Medical Academy Haydarpasa Training Hospital Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra020542"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pathol.1.110304.100125"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00054-05"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2005.00334.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004836-199604000-00004"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1998.tb02081.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.48.7.662"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00033-06"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-004-0794-0"},{"key":"e_1_2_9_11_1","first-page":"183","article-title":"Staging in rhinosinusitis.","volume":"31","author":"Lund VJ","year":"1993","journal-title":"Rhinology"},{"key":"e_1_2_9_12_1","first-page":"241","volume-title":"Current protocols in molecular biology.","author":"Wilson K","year":"1990"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.976-980.2003"},{"key":"e_1_2_9_14_1","first-page":"1","article-title":"Schistosomes, liver flukes and Helicobacter pylori. 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